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| 1 | Development of A Super-Sensitive Diagnostic Method for African Swine Fever Using CRISPR Techniques显示文摘African swine fever(ASF)is an infectious disease caused by African swine fever virus(ASFV)with clinical symptoms of high fever,hemorrhages and high mortality rate,posing a threat to the global swine industry and food security.Quarantine and control of ASFV is crucial for preventing swine industry from ASFV infection.In this study,a recombinase polymerase amplification(RPA)-CRISPR-based nucleic acid detection method was developed for diagnosing ASF.As a highly sensitive method,RPA-CRISPR can detect even a single copy of ASFV plasmid and genomic DNA by determining fluorescence signal induced by collateral cleavage of CRISPR-lw Cas13 a(previously known as C2c2)through quantitative real-time PCR(q PCR)and has the same or even higher sensitivity than the traditional q PCR method.A lateral flow strip was developed and used in combination with RPA-CRISPR for ASFV detection with the same level of sensitivity of Taq Man q PCR.Likewise,RPA-CRISPR is capable of distinguishing ASFV genomic DNA from viral DNA/RNA of other porcine viruses without any cross-reactivity.This diagnostic method is also available for diagnosing ASFV clinical DNA samples with coincidence rate of 100%for both ASFV positive and negative samples.RPA-CRISPR has great potential for clinical quarantine of ASFV in swine industry and food security. | Meishen Ren Hong Mei Ming Zhou Zhen F.Fu Heyou Han Dingren Bi Fuhu Peng Ling Zhao | 2021 | Virologica Sinica2021，36，2： | 5 |
| 2 | Genetic and Molecular Characterization of H9N2 Avian Influenza Viruses Isolated from Live Poultry Markets in Hubei Province, Central China, 2013–2017显示文摘H9N2 subtype avian influenza virus(AIV)is an influenza A virus that is widely spread throughout Asia,where it jeopardizes the poultry industry and provides genetic material for emerging human pathogens.To better understand the epidemicity and genetics of H9 subtype AIVs,we conducted active surveillance in live poultry markets(LPMs)in Hubei Province from 2013 to 2017.A total of 4798 samples were collected from apparent healthy poultry and environment.Realtime RT-PCR revealed that the positivity rate of influenza A was 26.6%(1275/4798),of which the H9 subtype accounted for 50.3%(641/1275)of the positive samples.Of the 132 H9N2 viral strains isolated,48 representative strains were subjected to evolutionary analysis and genotyping.Phylogenetic analysis revealed that all H9N2 viral genes had 91.1%–100%nucleotide homology,clustered with genotype 57,and had high homology with human H9N2 viruses isolated from2013 to 2017 in China.Using a nucleotide divergence cutoff of 95%,we identified ten distinct H9N2 genotypes that continued to change over time.Molecular analysis demonstrated that six H9N2 isolates had additional potential glycosylation sites at position 218 in the hemagglutinin protein,and all isolates had I155 T and Q226 L mutations.Moreover,44 strains had A558 V mutations in the PB2 protein and four had E627 V mutations,along with H9N2 human infection strains A/Beijing/1/2016 and A/Beijing/1/2017.These results emphasize that the H9N2 influenza virus in Hubei continues to mutate and undergo mammalian adaptation changes,indicating the necessity of strengthening the surveillance of the AIV H9N2 subtype in LPMs. | Zhibin Hu Fuhu Peng Zhenghui Xiong Wanpo Zhang Tingting Li Yuejun Shi Jun Xie Xin Jin Jingjing Huang Hongde Xiao Dingren Bi Nianhua Song Zili Li | 2021 | Virologica Sinica2021，36，2： | 4 |
| 3 | Recent Advances on Porous Materials for Synergetic Adsorption and Photocatalysis显示文摘Porous photocatalysts are promising materials capable of simultaneously adsorbing and oxidizing/reducing target species,showing great potentials in environmental remediation and energy generation.This review offered a comprehensive overview of the recent progress in design,fabrication,and applications of porous photocatalysts,including carbon-based semiconductors,metal oxides/sulfides,metal–organic frameworks,and adsorbent–photocatalyst hybrids.The fundamental understanding of the structure–performance relationships of porous materials together with the in-depth insights into the synergetic effects between adsorption and photocatalysis was presented.The strategies to further improve the photocatalytic activity of porous photocatalysts were proposed.This review would provide references and outlooks of constructing efficient porous materials for adsorptive and photocatalytic removal of pollutants and energy production. | Tianqi Wang Bingbing Tian Bin Han Dingren Ma Mingzhe Sun Aamir Hanif Dehua Xia Jin Shang | 2022 | Energy & Environmental Materials2022，5，3： | 2 |
| 4 | Development of a highly sensitive and specific monoclonal antibody-based enzyme-linked immunosorbent assay for determination of doxycycline in chicken muscle, liver and egg显示文摘 | Tao Le Zhengwu Zhao Wei Wei Dingren Bi | 2012 | Food Chemistry2012，，4： | 1 |
| 5 | Development and application of lateral flow test strip technology for detection of infectious agents and chemical contaminants: a review显示文摘 | Babacar Ngom Yancheng Guo Xiliang Wang Dingren Bi | 2010 | Analytical and Bioanalytical Chemistry2010，，3： | 1 |
| 6 | Haemophilus parasuis infection activates the NF-κB pathway in PK-15 cells through IκB degradation显示文摘 | Yushan Chen Hui Jin Ping Chen Zili Li Xianrong Meng Mei Liu Shaowen Li Deshi Shi Yuncai Xiao Xiliang Wang Zutao Zhou Dingren Bi Rui Zhou | 2012 | Veterinary Microbiology (-)2012，，1： | 1 |
| 7 | Influence of squalene feeding on plasma leptin, testosterone and blood pressure in rats显示文摘 | Yuxi Liu Xianhuan Xu Dingren Bi | 2009 | Indian J Med Res2009，，： | 1 |
| 8 | Resources of Vascular Plants in Guangxi Lagou Nature Reserve显示文摘Based on plant investigation of Guangxi Lagou Nature Reserve, the result indicated that the vascular plants are abundant, represented by 1,078 species(including varieties, subspecies), belonging to 657 genera in 186 families. Among these plants found, there are about 88 timber plants, 358 medicinal plants, 36 oil plants, 70 fi ber plants, 29 starch plants, 25 fruit plants, 28 aromatic plants, 39 tannic plants, 12 health beverage plants, 49 forage plants, 75 ornamental plants, 23 soil-conserving plants, 43 rare and endangered plants. It's urgent to protect the plant resources, so suggestions about conservation and sustainable utilization of the resources were put forward. | LUO Kaiwen PENG Dingren QIN Yonghua ZHANG Xianlai | 2015 | Journal of Landscape Research2015，7，2： | 1 |
| 9 | A NOTE ON CITY BLOCK DISTANCE显示文摘In this paper, problem of characterizing the city block distance between two lattice points in k-dimensional Euclidean space is discussed. | DINGREN XUCHANGQING LIYINGZI | 1998 | Applied Mathematics(A Journal of Chinese Universities)1998，13，3： | 0 |
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