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| 1 | Grain refinement and macrosegregation behavior of direct chill cast Al-Zn-Mg-Cu alloy under combined electromagnetic fields显示文摘The combined electromagnetic fields were achieved by the application of an alternating magnetic field and a stationary magnetic field and were used during direct chill(DC) casting process to control the microstructure and macrosegregation of an Al-Zn-Mg-Cu alloy. Ingot microstructures were analyzed under an optical microscope(Leica DMR). The composition at different locations in the ingots was measured with inductively coupled plasma mass spectrometry(ICP) method. The results showed that the grain structure is transformed from dendrite to equiaxed structure and significantly refined with the application of combined electromagnetic fields. The uniformity of microstructure is also greatly improved. The combined electromagnetic fields show a significant effect on the distribution of elements. The negative macrosegregation in the centre area of the ingot is obviously reduced. | Yu-bo Zuo Xu-dong Liu Chao Sun Sen-sen Yuan Dan Mou Zhan-zhi Li Jian-zhong Cui | 2015 | China Foundry2015，12，5： | 1 |
| 2 | Long non-coding RNA H19 regulates neurogenesis of induced neural stem cells in a mouse model of closed head injury显示文摘Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury. | Mou Gao Qin Dong Zhijun Yang Dan Zou Yajuan Han Zhanfeng Chen Ruxiang Xu | 2024 | Neural Regeneration Research2024，19，4： | 1 |
| 3 | SOX2 promotes tumorigenesis and increases the anti-apoptotic property of human prostate cancer cell显示文摘SRY-related HMG-box gene 2(SOX2)is one of the key regulatory genes that maintain the pluripotency and self-renewal properties in embryonic stem cells.Here we used immunohistochemistry to analyze the expression of SOX2 in human prostate tissues and found it contributed to tumorigenesis and correlated with histologic grade and Gleason score.We further investigated SOX2’s function in cell growth and apoptosis process by using a human prostate cancer cell line DU145 with SOX2 overexpression or down-regulation.Cell cycle assay revealed that SOX2 promoted cell growth and increased the percentage of cells in S phase.In vitro and in vivo xenograft experiments in NOD/SCID mice further demonstrated that SOX2 increased the apoptosis-resistant properties of DU145 cells with decreased function of store-operated Ca21 entry and reduced expression of Orai1 at both mRNA and protein levels,suggesting a potential mechanism that contributes to the anti-apoptotic property of SOX2.To our knowledge,this study is the first to investigate SOX2’s function in tumorigenesis and apoptosis of human prostate cancer and to elucidate its regulatory effect on the activity of store-operated Ca21 channels.Our results support the concept that SOX2 has the potential to be a significant marker to evaluate the progression of prostate cancer and serve as a potentially useful target for prostate cancer therapy. | Xianpei Jia Xuefei Li Yingxi Xu Shu Zhang Wenjun Mou Yanhua Liu Yin Liu Dan Lv Cheng-Hu Liu Xiaoyue Tan Rong Xiang Na Li | 2011 | Journal of Molecular Cell Biology2011，3，4： | 0 |
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