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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Exploring an Integrative Therapy for Treating COVID-19:A Randomized Controlled Trial显示文摘Objectives:To develop a new Chinese medicine(CM)-based drug and to evaluate its safety and effect for suppressing acute respiratory distress syndrome(ARDS)in COVID-19 patients.Methods:A putative ARDS-suppressing drug Keguan-1 was first developed and then evaluated by a randomized,controlled two-arm trial.The two arms of the trial consist of a control therapy(alpha interferon inhalation,50(x g twice daily;and lopinavir/ritonavir,400 and 100 mg twice daily,respectively)and a testing therapy(control therapy plus Keguan-119.4 g twice daily)by random number table at 1:1 ratio with 24 cases each group.After 2-week treatment,adverse events,time to fever resolution,ARDS development,and lung injury on newly diagnosed COVID-19 patients were assessed.Results:An analysis of the data from the first 30 participants showed that the control arm and the testing arm did not exhibit any significant differences in terms of adverse events.Based on this result,the study was expanded to include a total of 48 participants(24 cases each arm).The results show that compared with the control arm,the testing arm exhibited a significant improvement in time to fever resolution(P=0.035),and a significant reduction in the development of ARDS(P=0.048).Conclusions:Keguan-1-based integrative therapy was safe and superior to the standard therapy in suppressing the development of ARDS in COVID-19 patients.(Trial registration No.NCT 04251871 at www.clinicaltrials.gov). | WANG Jia-bo WANG Zhong-xia JING Jing ZHAO Peng DONG Jing-hui ZHOU Yong-feng YANG Guang NIU Ming ZHAO Xu JIANG Tian-jun Bl Jing-feng XU Zhe ZHANG Ping WU Dan BAI Zhao-fang GUO Yu-ming YU Si-miao SUN Yong-qiang ZHANG Zi-teng ZHAN Xiao-yan LI Peng-yan DING Jin-biao ZHAO Peng-fei SONG Xue-ai TANG Jian-yuan HE Dong-chu CHEN Zhu QIN En-qiang WANG Rui-lin XIAO Xiao-he | 2020 | Chinese Journal of Integrative Medicine2020，26，9： | 18 |
| 3 | Growth-inhibiting effects of taxol on human liver cancer in vitro and in nude mice显示文摘AIM To investigate the effects of taxol onSMMC-7721 human hepatoma and itsmechanisms.METHODS In vitro cell growth was assessedby trypan blue exclusion method.Experimentalhepatoma model was established by seedingSMMC-7721 cells subcutaneously into Balb/c(nu/nu)nude mice.In vivo tumor growth wasdetermined by measurement of tumor diameterwith Vernier calipers.The syntheses of DNA,RNA and protein were analyzed by incorporationof ~3H-thymidine,~3H-uridine and ~3H-leucinerespectively.Using light and electronmicroscopes to observe the morphologicalchanges of cells including mitosis andapoptosis.RESULTS Taxol was effective against SMMC-7721 human hepatoma cell growth in the rangesof 2.5 nmol/L-10 nmol/L with mitotic arrestand apoptosis in vitro.DNA,RNA and proteinsyntheses in cells were also obviouslysuppressed by in vitro treatment of taxol for72 h.Taxol at 2.5 nmol/L reduced ~3H-thymidineuptake to about 34% of the control value(P<0.05).Increasing the dose of taxol to20 nmol/L resulted in a greater decrease in ~3H-thymidine incorporation to 60% of the controlvalue(P<0.01).At a concentration of 20 nmol/L,the ~3H-uridine and ~3H-leucine uptakeswere reduced to 52%(P<0.05)and 63%(P<0.01),respectively.In vivo,taxolsignificantly inhibited SMMC-7721 tumor growthat 10 mg/kg,i.p.,once daily for 10 d.A morethan 90% decrease in tumor volume wasobserved by day 11(P<0.01)similarly withmitotic arrest and cell apoptosis.CONCLUSION Taxol has a marked anticanceractivity in SMMC-7721 human hepatoma both invitro and in nude mice.Its mechanisms might beassociated with mitotic arrest,subsequently,apoptosis of the hepatoma cells.No obvioustoxicity was observed with in vivoadministration of taxoi. | Jin Hui Yuan Ru Ping Zhang Ru Gang Zhang Li Xia Guo Xing Wang Wang Dan Luo Yong Xie Hong Xie | 2000 | World Journal of Gastroenterology2000，6，2： | 18 |
| 4 | A comprehensive review of integrative pharmacology-based investigation:A paradigm shift in traditional Chinese medicine显示文摘Over the past decade,traditional Chinese medicine(TCM) has widely embraced systems biology and its various data integration approaches to promote its modernization.Thus,integrative pharmacology-based traditional Chinese medicine(TCMIP) was proposed as a paradigm shift in TCM.This review focuses on the presentation of this novel concept and the main research contents,methodologies and applications of TCMIP.First,TCMIP is an interdisciplinary science that can establish qualitative and quantitative pharmacokinetics-pharmacodynamics(PK-PD) correlations through the integration of knowledge from multiple disciplines and techniques and from different PK-PD processes in vivo.Then,the main research contents of TCMIP are introduced as follows:chemical and ADME/PK profiles of TCM formulas;confirming the three forms of active substances and the three action modes;establishing the qualitative PK-PD correlation;and building the quantitative PK-PD correlations,etc.After that,we summarize the existing data resources,computational models and experimental methods of TCMIP and highlight the urgent establishment of mathematical modeling and experimental methods.Finally,we further discuss the applications of TCMIP for the improvement of TCM quality control,clarification of the molecular mechanisms underlying the actions of TCMs and discovery of potential new drugs,especially TCM-related combination drug disco very. | Haiyu Xu Yanqiong Zhang Ping Wang Junhong Zhang Hong Chen Luoqi Zhang Xia Du Chunhui Zhao Dan Wu Feng Liu Hongjun Yang Changxiao Liu | 2021 | Acta Pharmaceutica Sinica B2021，11，6： | 18 |
| 5 | Soil Bacterial Communities Under Different Long-Term Fertilization Regimes in Three Locations Across the Black Soil Region of Northeast China显示文摘Although soil bacteria play critical roles in agro-ecosystems, the knowledge of their response to long-term fertilization across the black soil region of Northeast China is limited. In this study, we sequenced 16 S rRNA genes to assess the effects of four long-term fertilization regimes—non-fertilization(NoF), chemical fertilizer(CF), manure(M), and chemical fertilizer plus manure(CFM)—on soil properties and bacterial communities in three locations, the northern, middle, and southern parts, across the black soil region. Results showed that the influence of fertilization regimes on soil properties varied significantly among the three locations. Manure fertilization significantly increased microbial biomass carbon and relative abundance of copiotrophic bacteria. Principal component analysis(PCA)showed that the total bacterial communities were separated into three groups according to the sampling location despite long-term fertilization, and that soil pH was the most important factor in shifting bacterial communities. In addition, similar fertilization regimes resulted in different influences on bacterial community composition, and the most influential soil properties varied among the three locations. Our results highlighted that geographical separation was a more dominant factor affecting bacterial communities than fertilization, and that long-term similar fertilization regimes did not induce consistent changes in bacterial community composition in the black soil region. | HU Xiaojing LIU Junjie WEI Dan ZHU Ping CUI Xi'an ZHOU Baoku CHEN Xueli JIN Jian LIU Xiaobing WANG Guanghua | 2018 | Pedosphere2018，28，5： | 17 |
| 6 | Effect and mechanism of Irbesartan on occurrence of ventricular arrhythmias in rats with myocardial ischemia through connexin43(cx43)显示文摘Objective: To explore the effect and mechanism of angiotensin Ⅱ receptor blockers-Irbesartan on occurrence of ventricular arrhythmias in rats with myocardial ischemia. Methods: Rats with embryonic cardiomyocytes-H9c2 were randomly divided into control group, ischemia group, Irbesartan group and Irbesartan+ischemia group. The cell viability of rats in each group was tested using MTT. Real-time PCR was employed to detect the expression of connexin43(Cx43) mR NA and western blot to detect the expression of Cx43 and phosphorylated Cx43. SD rats were randomly divided into the sham-operation group(SO), myocardial infarction group(MI), Irbesartan group and MI+ Irbesartan group, with 10 rats in each group. HE staining was employed to observe the change in the pathomorphology of left ventricular tissue and TUNEL method to analyze the cell apoptosis in the tissue. The immunofluorescence was adopted to observe the expression and distribution of Cx43 in the left ventricular myocardium and study the change in the expression of Cx43 in the cardiac muscular tissue at mR NA and protein level. Results: The intervention of Irbesartan in the condition of ischemia indicated the significant decrease in the number of necrotic cells. The expression of Cx43 was significantly decreased under the culture of ischemia(P<0.05), but in the presence of Irbesartan, the expression of Cx43 was increased compared with the ischemia group(P<0.01). The results of WB assay showed the similar trend of change at mRNA level. There was the significant difference in the score of ventricular arerythmia between MI group and SO group(P<0.01). The incidence of ventricular tachycardia or ventricular fibrillation was significantly increased compared with the one in SO group(P<0.05). There was the significant difference in the overall score between MI+Irbesartan group and MI group(P<0.05). The expression of Cx43 in the cardiac muscular tissue in MI group was significantly decreased(P<0.01 vs SO group). But the expression of Cx43 was increased after the treatment with Irbesartan. Conclusions: Irbesartan can inhibit the injury of H9c2 cardiomyocytes and the decreased expression of Cx43 that are induced by the ischemic myocardial infarction. Irbesartan can also improve the reconstruction of Cx43 in rats with ischemic myocardium to inhibit the myocardial infarction-induced arrhythmias. | Tao Wu Dan Wu Qinghua Wu Bing Zou Xiao Huang Xiaoshu Cheng Yanqing Wu Kui Hong Ping Li | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，10： | 15 |
| 7 | NK4基因转染对前列腺癌DU145细胞增殖、迁移、侵袭和凋亡的影响显示文摘研究HGF拮抗剂NK4在前列腺癌中的作用。将包含NK4cDNA的表达载体pBudCE4.1-EGFP-NK4转染到DU145细胞中。体外实验检测白分泌的NK4对肿瘤细胞增殖、迁移、转移及凋亡的影响。体内实验裸鼠分为三组,分别皮下种植DUl45、空质粒转染的Dul45和NK4转染的DUl45细胞,检测皮下肿瘤的大小、细胞凋亡及细胞增殖情况。体外实验结果显示转染NK4的DUl45细胞可以分泌NK4蛋白。自分泌的NK4抑制HGF诱导的肿瘤细胞增殖、迁移及转移,促进凋亡(P〈0.01)。NK4可以调节HGF受体c-Met及其下游ERKl与Akt1/2蛋白的活性。体内实验显示,NK4转染DUl45细胞组的肿瘤生长及细胞增殖受到抑制,同时肿瘤细胞凋亡增加。本实验显示包含NK4cDNA的表达载体转染前列腺癌细胞可以有效地调节肿瘤细胞的增殖、迁移、侵袭及凋亡。NK4作用于HGF/c-Met可以作为前列腺癌治疗的一个有效靶点。 | Dan Yue Yong Wang Ping Ma Yin-Yan Li Hong Chen Ping Wang Chang-Shan Ren | 2010 | Asian Journal of Andrology2010，12，3： | 14 |
| 8 | Prognostic evaluation of postoperative adjuvant therapy for operable cervical cancer:10 years'experience of National Cancer Center in China显示文摘Objective: The aim of this study was to investigate the prognostic factors and to evaluate the impact of adjuvant therapy on clinical outcome for early-stage cervical cancer.Methods: The clinical-pathological data of all 1,335 patients with the International Federation of Gynecology and Obstetrics(FIGO) Ib–IIa cervical cancer treated with primary radical surgery at the Chinese National Cancer Center between May 2007 and Dec 2013 were retrospectively reviewed.The median follow-up was 70 months.Results: Of all the patients,61.6% of the cases received adjuvant therapy,with 5-year disease-free survival(DFS)of 92.1% and 5-year overall survival(OS) of 95.0%.In multivariate analysis,differentiation of G3(P<0.05),lymph node metastasis(LNM,P<0.05) and lymphovascular space invasion(LVSI,P<0.05) were independent predictors for OS,while LNM(P<0.05),deep stroma invasion(DSI,P<0.05) and LVSI(P<0.05) were independent factors for DFS.The samples were stratified by histologic type,and cervical squamous cell carcinoma(SCC) was found to share the same independent factors except for differentiation of OS.As to patients with cervical adenocarcinoma/adenosquamous carcinoma(AC/ASC),differentiation was the independent predictor of OS(P<0.05); and LVSI of DFS(P<0.05).Of 236 patients with high-risk factors,there was no significant difference in survival between concurrent chemoradiotherapy(CCRT,n=195),radiotherapy(RT,n=24),and chemotherapy(CT,n=17).Among the 190 patients with LNM who underwent CCRT,124 cases showed improved DFS after sequential CT(P=0.118),with a recurrence rate decrease of 14%,though the difference was not statistically significant.Patients with single intermediate-risk factors like DSI or LVSI were found to partially benefit from adjuvant therapy,but the difference was not statistically significant.Conclusions: LNM,LVSI,DSI and differentiation were found to be independent prognostic factors for operable cervical cancer.Aggressive postoperative adjuvant therapy based on single risk factors in Chinese National Cancer Center could benefit survival.CCRT+CT outperformed CCRT in high-risk patients.For patients with single non-high-risk factor,the role of adjuvant therapy needs to be further discussed. | Tong Shu Dan Zhao Bin Li Yating Wang Shuanghuan Liu Pingping Li Jing Zuo Ping Ba i RongZhang Lingying Wu | 2017 | Chinese Journal of Cancer Research2017，29，6： | 12 |
| 9 | Associations of plasma homocysteine and high-sensitivity C-reactive protein levels with arterial stiffness in Chinese population: a community-based study显示文摘背景动脉的僵硬与年龄增加并且是也与传统的心血管的风险 factors.Little 联系了在学习是与动脉的僵硬调查血浆 homocysteine 和 hs-CRP 层次的协会在里面的礼品的中国 community.The 目的对与动脉的僵硬的 homocysteine 和高敏感的C反应的蛋白质( hs-CRP )的关系被知道我们联系了的基于社区的 cohort.Methods homocysteine 和 hs-CRP 铺平到动脉的 stiffne | FENG Sheng-qiang YE Ping LUO Lei-ming XIAO Wen-kai BAI Yong-yi FENG Dan LIU De-jun WU Hong-mei XU Ru-yi BAI Jie | 2012 | Chinese Medical Journal2012，，1： | 12 |
| 10 | Chinese herbal medicine reduces mortality in patients with severe and critical coronavirus disease 2019: a retrospective cohort study显示文摘This study aimed to evaluate the efficacy of Chinese herbal medicine(CHM)in patients with severe/critical coronavirus disease 2019(COVID-19).In this retrospective study,data were collected from 662 patients with severe/critical COVID-19 who were admitted to a designated hospital to treat patients with severe COVID-19 in Wuhan before March 20,2020.All patients were divided into an exposed group(CHM users)and a control group(non-users).After propensity score matching in a 1:1 ratio,156 CHM users were matched by propensity score to 156 non-users.No significant differences in seven baseline clinical variables were found between the two groups of patients.All-cause mortality was reported in 13 CHM users who died and 36 non-users who died.After multivariate adjustment,the mortality risk of CHM users was reduced by 82.2%(odds ratio 0.178,95%CI 0.076–0.418;P<0.001)compared with the non-users.Secondly,age(odds ratio 1.053,95%CI 1.023–1.084;P<0.001)and the proportion of severe/critical patients(odds ratio 0.063,95%CI 0.028–0.143;P<0.001)were the risk factors of mortality.These results show that the use of CHM may reduce the mortality of patients with severe/critical COVID-19. | Guohua Chen Wen Su Jiayao Yang Dan Luo Ping Xia Wen Jia Xiuyang Li Chuan Wang Suping Lang Qingbin Meng Ying Zhang Yuhe Ke An Fan Shuo Yang Yujiao Zheng Xuepeng Fan Jie Qiao Fengmei Lian Li Wei Xiaolin Tong | 2020 | Frontiers of Medicine2020，14，6： | 10 |
| 11 | A new iridoid glycoside from Gardenia jasminoides显示文摘新 iridoid glycoside, 6 ′ - O-sinapoylgeniposide,从栀子 jasminoides 埃利斯被孤立,它的结构根据 1D 和 2D NMR 被阐明, HR-ESI-MS 技术。 | Xiao Qin Zhou Zhi Ming Bi Ping Li Dan Tang Hal Xia Cai | 2007 | Chinese Chemical Letters2007，18，10： | 10 |
| 12 | The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells显示文摘Hepatocyte 生长因素(HGF ) 是导致前列腺癌症细胞增长,移植和侵略的 glycoprotein。短暂受体潜力的激活正规 6 (TRPC6 ) 隧道在支持前列腺癌症房间增长被认为重要。在这研究,我们在前列腺癌症的导致 HGF 的房间增长估计了内长的 TRPC6 隧道的角色。反向的 transcription-PCR 并且西方的弄污被用来调查 TRPC6 表示。Electrophysiological 技术(整个房间的补丁夹钳配置) 并且 Ca2+ 成像分析被用来在房间调查隧道活动。房间周期前进,房间 apoptosis 和房间生长上的 TRPC6 隧道的效果也被检验。TRPC6 并且遇见 c 在 DU145 和 PC3 房间被表示。另外,功能的 TRPC6 隧道在击倒的压制的象 TRPC 一样水流由 oleoyl-2-acetyl-sn-glycerol (OAG ) 唤起了的 DU145 和 PC3 房间,和 TRPC6 是在场的。在 DU145 和 PC3 房间的 TRPC6 隧道的抑制废除了导致 OAG 、导致 HGF 的 Ca2+ 入口。而且, TRPC6 隧道的抑制在 G2/M 阶段逮捕了 DU145 和 PC3 房间并且压制了导致 HGF 的房间增长。一起,我们的结果显示 TRPC6 在导致 HGF 的 DU145 和 PC3 房间增长有一个重要角色。 | Yong Wang Dan Yue Kai Li Yi-Li Liu Chang-Shah Ren Ping Wang | 2010 | Asian Journal of Andrology2010，12，6： | 10 |
| 13 | Fluoxetine ameliorates symptoms of refractory chronic prostatitis/chronic pelvic pain syndrome显示文摘背景范畴长期的 prostatitis/chronic 骨盆的疼痛症候群(CP/CPPS ) 是有生活的质量上的重要影响的不清楚的病原学的普通症候群。因为为 CP/CPPS 的多重治疗的结果远离同意,可能的心理因素被认为在这上在 CP/CPPS.Based 起一个重要作用,我们在这研究与倔强的 CP/CPPS.Methods 在人调查了抗抑郁剂药( fluoxetine )的角色,没有对标准治疗的反应,与倔强的 CP/CPPS 诊断的 42 个人(包括多重抗菌素功课和 a-blockers )为 fluoxetine 被提交治疗。所有病人收到了 fluoxetine (20 mg/d ) 三个月并且以前临床上被评估(基线) ,并且在治疗的 4, 8 和 12 个星期以后。评估包括了健康长期的前列腺炎症状索引(NIH-CPSI ) 和贝克萧条的研究所盘点的一个公民(BDI ) 问询表。而且,主观全球评价( SGA )在 therapy.Results 的 第4 , 第8 和 第12 星期被估计重要减少为全部的 NIH-CPSI ( 28.55~9.29 )被观察, NIH-CPSI 疼痛( 14.69~5.19 ), NIH-CPSI 尿( 4.95~1.88 ),生活( 8.83~2.20 )的 NIH-CPSI 质量,和 BDI ( 34.67~13.95 )与基线相比得分,所有 P 珍视 0.05 。(69.05%) 29 在主观全球评价以后报导了显著改进,(78.57%) 33 有一比在在治疗(第 12 星期) 的结束的 NIH-CPSI 的 50% 减少大。同时,皮尔森关联系数分析表明了在 BDI 分数和每个 CPSI 分数之间的积极关联。没有不利事件在这 study.Conclusions Fluoxetine 被报导看起来是在改善的安全、有效的治疗症状在,和生活的质量,有困难的 CP/CPPS 的人。而且,困难的 CP/CPPS-related 症状的改善能与在压抑的症状的减少有关。 | XIA Dan WANG Ping CHEN Jun WANG Shuo JIANG Hai | 2011 | Chinese Medical Journal2011，，14： | 10 |
| 14 | TRPC6在人良性与恶性前列腺组织中的表达显示文摘观察瞬时受体电位通道C6(TRPC6)在人良性与恶性前列腺组织及前列腺癌细胞系中的表达,进一步探讨TRPC6的表达与前列腺癌分期、分级及激素依赖性的关系。利用免疫组织化学技术,检测发现45.0%的前列腺增生和86.6%的前列腺癌病例表达TRPC6,两者比较有显著性差异沪〈0.01)。TRPC6的表达与前列腺癌分级和前列腺外转移有关(P〈0.01)。前列腺癌分期增高,TRPC6表达增多,但在T2、T3和DT4期肿瘤病例中,TRPC6表达无显著差异。此外TRPC6在激素依赖性前列腺癌与激素非依赖性前列腺癌中的表达也无显著差异。应用RT-PCR及Westernblot,检测到TRPC6在前列腺癌细胞系中的表达。本研究发现,TRPC6在良性与恶性前列腺组织及前列腺癌细胞系中表达。TRPC6的表达与前列腺癌的组织分级、Gleason评分及前列腺外转移有关。 | Dan Yue Yong Wang Jian-Ying Xiaot Ping Wang Chang-Shan Ren | 2009 | Asian Journal of Andrology2009，11，5： | 8 |
| 15 | Regulation of Survivin and CDK4 by Epstein-Barr virus encoded latent mem-brane protein 1 in nasopharyngeal carcinoma cell lines显示文摘Latent membrane protein 1 (LMP1), an important protein encoded by Epstein Barr virus (EBV), has been implied to link with the pathogenesis of nasopharyngeal carcinoma (NPC). Its dual effects of increasing cell proliferation and inhibiting cell apoptosis have been confirmed. In this study, we showed that the expression of Survivin and CDK4 protein in CNE-LMP1, a LMP1 positive NPC epithelial cell line, is higher than in LMP1 negative NPC epithelial cell line- CNE1, and the expression is LMP1 dosage-dependent. Although it was reported that Survivin specifically expressed in cell cycle G2/M phase, our studies suggested that LMP1 could promote the expression of Survivin in G0/G1, S and G2/ M phase. It also showed that Survivin and CDK4 could be accumulated more in the nuclei triggered by LMP1. More interestingly, Survivin and CDK4 could form a protein complex in the nuclei of CNE-LMP1 rather than in that of CNE1, which demonstrated that the interaction between these two proteins could be promoted by LMP1. These results strongly suggested that the role of LMP1 in the regulation of Survivin and CDK4 may also shed some light on the mechanism research of LMP1 in NPC. | Mi Dan AI Li Li LI Xiao Rong ZHAO Yong WU Jian Ping GONG Ya CAO | 2005 | Cell Research2005，15，10： | 8 |
| 16 | Relationship between Maternal PBMC HBV cccDNA and HBV Serological Markers and its Effect on HBV Intrauterine Transmission显示文摘Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and its effects on HBV intrauterine transmission. Methods We enrolled 290 newborns and their hepatitis B surface antigen(HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real‐time PCR‐TaqM an probe while HBV serological markers were detected with an electrochemiluminescence immunoassay. Results There was a positive correlation between the levels of PBMC HBV cccD NA and serum HBV DNA and HBeA g(r = 0.436 and 0.403, P < 0.001). The detection rate of pattern A [‘HBsA g(+), HBeA g(+), and anti‐HBc(+)’] was significantly higher in the PBMC HBV cccD NA positive group than in the control group(χ^2 = 48.48, P < 0.001). There was a significant association between HBV intrauterine transmission and PBMC HBV cccD NA(χ^2 = 9.28, P = 0.002). In the presence of serum HBV DNA, HBeA g, and PBMC HBV cccD NA, the risk of HBV intrauterine transmission was three times higher(OR = 3.69, 95% CI: 1.30‐10.42) than that observed in their absence. The risk of HBV intrauterine transmission was the greatest(OR = 5.89, 95% CI: 2.35‐14.72) when both PBMC HBV cccD NA and pattern A were present. A Bayesian network model showed that maternal PBMC HBV cccD NA was directly related to HBV intrauterine transmission. Conclusion PBMC HBV cccDNA may be a direct risk factor for HBV intrauterine transmission. Our study suggests that serological markers could be combined with PBMC‐related markers in prenatal testing. | WANG Dan Dan YI Lin Zhu WU Li Na YANG Zhi Qing HAO Hai Yun SHI Xiao Hong WANG Bo FENG Shu Ying FENG Yong Liang WANG Su Ping | 2019 | Biomedical and Environmental Sciences2019，32，5： | 8 |
| 17 | Detection of serum IgM and IgG for COVID-19 diagnosis显示文摘Dear Editor,Infection with the novel coronavirus(SARS-CoV-2,which is the virus responsible for the coronavirus disease 2019(COVID-19))was first reported in Wuhan,China on December 31,2019.The outbreak of COVID-19 remains ongoing and was linked to more than 80,000 infected patients and more than 3,000 deaths in China as of March 7,2020(Holshue et al.,2020). | Ling Zhong Junlan Chuan Bo Gong Ping Shuai Yu Zhou Yi Zhang Zhilin Jiang Dingding Zhang Xiaoqi Liu Shi Ma Yi Huang He Lin Qingwei Wang Lulin Huang Dan Jiang Fang Hao Juan Tang Chunqi Zheng Hua Yu Zhibin Wang Qi Jiang Tao Zeng Mei Luo Fanwei Zeng Fanxin Zeng Jianghai Liu Junxi Tian Yu Xu Tengxiang Long Kaiju Xu Xingxiang Yang Yuping Liu Yi Shi Li Jiang Zhenglin Yang | 2020 | Science China(Life Sciences)2020，63，5： | 8 |
| 18 | Simultaneous catalytic hydrolysis of carbonyl sulfide and carbon disulfide over Al_2O_3-K/CAC catalyst at low temperature显示文摘In this work, a series of coal-based active carbon(CAC) catalysts loaded by Al2O3were prepared by sol-gel method and used for the simultaneous catalytic hydrolysis of carbonyl sulfide(COS) and carbon disulfide(CS2) at relatively low temperatures of 30-70 ℃. The influences of calcinations temperatures and operation conditions such as: reaction temperature, O2concentration, gas hourly space velocity(GHSV) and relative humidity(RH) were also discussed respectively. The results showed that catalysts with 5.0 wt% Al2O3calcined at 300 ℃ had superior activity for the simultaneous catalytic hydrolysis of COS and CS2. When the reaction temperature was above 50 ℃, catalytic hydrolysis activity of COS could be enhanced but that of CS2was inhibited. Too high RH could make the catalytic hydrolysis activities of COS and CS2decrease. A small amount of O2introduction could enhance the simultaneous catalytic hydrolysis activities of COS and CS2. | Xin Sun Ping Ning Xiaolong Tang Honghong Yi Kai Li Dan He Xianmang Xu Bin Huang Ruiyun Lai | 2014 | Journal of Energy Chemistry2014，23，2： | 7 |
| 19 | Two new saponins from the aerial part of Astragalus membranaceus var. mongholicus显示文摘Two new saponins named mongholicoside A (1) and mongholicoside B (2) were isolated from the aerial part of Astragalus membranaceus var. mongholicus. Their structures were determined by 1D and 2D NMR, ESI-MS techniques and chemical methods. | Qing Tao Yu Ping Li Zhi Ming Bi Jun Luo Xiao Dan Gao | 2007 | Chinese Chemical Letters2007，18，5： | 7 |
| 20 | Central Obesity and Metabolic Risk Factors in Middle-aged Chinese显示文摘Objective Central obesity is considered to be a central component of metabolic syndrome. Waist circumference(WC) has been widely used as a simple indicator of central obesity. This study is aimed to evaluate the sensitivity of WC cut-off values for predicting metabolic risk factors in middle-aged Chinese. Methods The study involved 923 subjects aged 40-65 years. The metabolic risk factors were defined according to the Chinese Joint Committee for Developing Chinese Guidelines on Prevention and Treatment of Dyslipidemia in Adults. WC cut-off 85-90 cm and ≥90 cm were used as cut-off values of central pre-obesity and central obesity in males, respectively, while WC 80-85 cm and ≥85 cm were used as cut-off values of central pre-obesity and central obesity in females. Results First, WC values corresponding to body mass index(BMI) 24 kg/m2 and visceral fat area(VFA) 80 cm2 were 88.55 cm and 88.51 cm in males, and 81.46 cm and 82.51 cm in females respectively. Second, receiver operating characteristic curves showed that the optimal WC cut-off of value was 88.75 cm in males, higher than that in females(81.75 cm). Third, the subjects with higher WC values were more likely to have accumulating metabolic risk factors. The prevalence of metabolic risk factors increased linearly and significantly in relation to WC levels. Conclusion WC cut-off values of central pre-/central obesity are optimal to predict multiple metabolic risk factors. | YIN Xue Yao ZHENG Fen Ping ZHOU Jia Qiang DU Ying PAN Qian Qian ZHANG Sai Fei YU Dan LI Hong | 2014 | Biomedical and Environmental Sciences2014，27，5： | 6 |
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