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| 1 | Pharmacopoeia of the People's Republic of China(2010 Edition):A Milestone in Development of China's Healthcare显示文摘Compilation of the Pharmacopoeia of the People's Republic of China (known as the Chinese Pharmacopoeia 2010) has been completed by the Ninth Chinese Pharmacopoeia Commission, which has been officially distributed in January 2010 and implemented on October 1st, 2010. | QIAN Zhong-zhi1,DAN Yang2,LIU Yan-ze2,PENG Yong2 1.Chinese Pharmacopoeia Commission,Beijing 100061,China 2.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China | 2010 | Chinese Herbal Medicines2010，2，2： | 55 |
| 2 | Bacterial Community Structure and Diversity in a Black Soil as Affected by Long-Term Fertilization显示文摘Black soil(Mollisol) is one of the main soil types in northeastern China. Biolog and polymerase chain reactiondenaturing gradient gel electrophoresis(PCR-DGGE) methods were used to examine the infiuence of various fertilizer combinations on the structure and function of the bacterial community in a black soil collected from Harbin,Heilongjiang Province. Biolog results showed that substrate richness and catabolic diversity of the soil bacterial community were the greatest in the chemical fertilizer and chemical fertilizer+manure treatments. The metabolic ability of the bacterial community in the manure treatment was similar to the control. DGGE fingerprinting indicated similarity in the distribution of most 16S rDNA bands among all treatments,suggesting that microorganisms with those bands were stable and not infiuenced by fertilization. However,chemical fertilizer increased the diversity of soil bacterial community. Principal component analysis of Biolog and DGGE data revealed that the structure and function of the bacterial community were similar in the control and manure treatments,suggesting that the application of manure increased the soil microbial population,but had no effect on the bacterial community structure. Catabolic function was similar in the chemical fertilizer and chemical fertilizer+manure treatments,but the composition structure of the soil microbes differed between them. The use of chemical fertilizers could result in a decline in the catabolic activity of fast-growing or eutrophic bacteria. | WEI Dan YANG Qian ZHANG Jun-Zheng WANG Shuang CHEN Xue-Li ZHANG Xi-Lin LI Wei-Qun | 2008 | Pedosphere2008，18，5： | 45 |
| 3 | Ten cities cross-sectional questionnaire survey of children asthma and other allergies in China显示文摘Asthma,rhinitis and eczema(allergic or non-allergic)have increased throughout the world during the last decades,especially among children.Changes in the indoor environment are suspected to be important causes.China has experienced a dramatic change in indoor environmental exposures during the past two decades.However,such changes and their associations with children’s asthma and other health aspects have not been thoroughly studied.China,Children,Homes,Health(CCHH),Phase I,was a cross-sectional questionnaire survey of 48219 children 1–8 years old in 10 Chinese cities during 2010–2012.The questionnaire includes the International Study of Asthma and Allergies in Childhood(ISAAC)core health questions and additional questions regarding housing,life habits and outdoor environment.In health analyses,children aged 3–6 years old were included.The prevalences of doctor diagnosed asthma varied from 1.7%to 9.8%(mean 6.8%),a large increase from 0.91%in 1999 and 1.50%in2000.The prevalence of wheeze,rhinitis and atopic eczema(last 12 months)varied from 13.9%to 23.7%,24.0%to 50.8%and4.8%to 15.8%,respectively.Taiyuan had the lowest prevalences of all illnesses and Shanghai the highest,except for wheezewhere the highest value was for Urumqi.We found(1)no obvious association between disease prevalences and ambient PM10concentrations and(2)higher prevalences of disease in humid climates with hot summers and cold winters,but with no centrally heated buildings.Associations between the diseases and economic status as indexed by Gross Domestic Product(GDP)requires further study. | ZHANG YinPing LI BaiZhan HUANG Chen YANG Xu QIAN Hua DENG QiHong ZHAO ZhuoHui LI AnGui ZHAO JiaNing ZHANG Xin QU Fang HU Yu YANG Qin WANG Juan ZHANG Ming WANG Fang ZHENG XiaoHong LU Chan LIU ZhiJian SUN YueXia MO JinHan ZHAO YiLi LIU Wei WANG TingTing NORBCK Dan BORNEHAG Carl-Gustaf SUNDELL Jan | 2013 | Chinese Science Bulletin2013，58，34： | 22 |
| 4 | Correlation Between the Clinical Severity, Bacterial Load, and Inflammatory Reaction in Children with Mycoplasma Pneumoniae Pneumonia显示文摘Given the lack of defining features in the clinical manifestations and radiographic findings for children with mycoplasma pneumoniae pneumonia(MPP),quantitative polymerase chain reaction(qPCR)has become a useful diagnostic method.This study was performed to explore the relationship between the qPCR findings,clinical symptoms,and inflammatory markers in children with MPP.Four hundred children with MPP have been enrolled in this retrospective analysis.All clinical and analytical information,including mycoplasma pneumoniae(MP)PCR results,has been collected.Based on the PCR results,the patients were divided into groups with load values(copy number)<105(54 cases),2105 and<106(71 cases),2106 and<107(112 cases),>107 and<108(114 cases),and>108(49 cases).The clinical features(including symptoms and signs)and inflammatory indicators were compared among the groups.The incidence of high fever(above 39℃),thermal peak during the entire hospitalization period,fever duration,days of hospitalization,and plasma lactate dehydrogenase(LDH)levels were statistically correlated with the MP PCR load value in children with MPP.The analysis of relevance degree showed the correlative order as a thermal peak of hospitalization>duration of fever>period of hospitalization>LDH value>C-reactive protein value.The host immune response was significantly greater in the complication group than in the non-complication group. | Chen ZHANG Qiao ZHANG Jie-lin DU Dan DENG Ye-lei GAO Cheng-lin WANG Hong-jie ZHAO Qian GUO Zhou FU Dai-yin TIAN | 2020 | Current Medical Science2020，40，5： | 19 |
| 5 | New Collection of Crude Drugs in Chinese Pharmacopoeia 2010 Ⅰ.Callicarpa Linn. and Related Items显示文摘Callicarpa Linn. (beautyberry) is one of the major genera in Verbenaceae, about 20 of which are medicinal plants. Beautyberry, called Zizhu in China, is a generic name of those species and largely used as hemostatic medicine. The Chinese Pharmacopoeia 2010 has admitted three new crude drugs from the genus of Callicarpa Linn. including Callicarpae Macrophyllae Folium, Callicarpae Caulis et Folium, and Callicarpae Formosanae Folium for the first time since the 1977 version of the Chinese Pharmacopoeia. In order to better understand these new crude drugs, we systematically described their bibliography, admission reasons, botanical identification, chemistry, and pharmacology. Several other species, out of national regulations but intensively studied and widely used, are also covered in this review. | DAN Yang1, QIAN Zhong-zhi2, LIU Yan-ze1, ZHOU Guo-ping3, PENG Yong1, XIAO Pei-gen1 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Beijing 100193, China 2. China Pharmacopoeia Committee, Beijing 100061, China 3. Jiangxi Provincial Institute for Food and Drug Control, Nanchang 330046, China | 2010 | Chinese Herbal Medicines2010，2，4： | 18 |
| 6 | Chinese Medicine Sequential Therapy Improves Pregnancy Outcomes after Surgery for Endometriosis-Associated Infertility: A Multicenter Randomized Double-blind Placebo Parallel Controlled Clinical Trial显示文摘Objective:To evaluate the efficacy and safety of Chinese medicine(CM)improving pregnancy outcomes after surgery for endometriosis-associated infertility.Methods:A multicenter,randomized,doubleblind placebo parallel controlled clinical trial was designed.A total of 202 patients who had laparoscopy for endometriosis-associated infertility with qi stagnation and blood stasis syndrome were included and randomly divided into the CM treatment group and placebo control group at a ratio of 1:1 using a central block randomization from May 2014 to September 2017,101 patients in each group.The two groups received continuous intervention at 1–5 days after surgery,for 6 menstrual cycles.Before ovulation,the CM group was treated Huoxue Xiaoyi Granule(活血消异颗粒);after ovulation,Bushen Zhuyun Granule(补肾助孕颗粒)was involved.The control group was treated with placebo.Transvaginal ultrasonography was performed every menstrual cycle during the treatment,and female hormone levels in the follicular and luteal phases were measured during the 1 st,3 rd and 6 th menstrual cycles.The analysis was continued until pregnancy.The primary outcomes were clinical pregnancy rate and pregnancy outcome,and the secondary outcomes were follicular development and endometrial receptivity.Safety evaluations were performed before and after treatment.Results:(1)Clinical pregnancy and live birth rates:the clinical pregnancy and live birth rates of the CM group were significantly higher than those of the placebo group[44.6%(45/101)vs.29.7%(30/101),34.7%(35/101)vs.20.8%(21/101),both P<0.05].(2)Follicle development:the incidence of dominant follicles,rate of cumulative cycle ovulation,and rate of cumulative cycle mature follicle ovulation were significantly higher in the CM group than those in the placebo group[93.8%(350/373)vs.89.5%(341/381),80.4%(275/342)vs.69.1%(253/366),65.8%(181/275)vs 56.1%(142/253),P<0.05 or P<0.01]).The incidence of cumulative cycle luteinized unruptured follicle syndrome was significantly lower in the CM group than in the placebo group[11.7%(40/342)vs.17.8%(65/366),P<0.05).(3)Endometrial receptivity:after treatment,both endometrial types and endometrial blood flow types in the CM group were mainly types A and B,while those in the placebo group were mainly types B and C,with a significant difference between the two groups(both P<0.05).(4)Adverse events:the incidence of adverse events between the two groups was not significantly different(P>0.05).Conclusion:Strategies for activating blood circulation-regulating Gan(Liver)-tonifying Shen(Kidney)sequential therapy can effectively improve the clinical pregnancy rate and live birth rate of endometriosis-associated infertility with qi stagnation and blood stasis after laparoscopy,improve follicular development,promote ovulation,improve endometrial receptivity,while being a safe treatment option.(Trial registration No.NCT02676713). | ZHAO Rui-hua LIU Yong LU Dan WU Ying WANG Xiao-yun LI Wei-li ZENG Cheng MENG Qing-wei LIAN Feng-mei ZHOU Jun SHI Yun SUN Wei-wei HAN Qian TANG Yi SHI Guang | 2020 | Chinese Journal of Integrative Medicine2020，26，2： | 13 |
| 7 | The prognostic value of serum C-reactive protein-bound serum amyloid A in early-stage lung cancer显示文摘Background:Elevated levels of serum C-reactive protein(CRP) have been reported to have prognostic significance in lung cancer patients.This study aimed to further identify CRP-bound components as prognostic markers for lung cancer and validate their prognostic value.Methods:CRP-bound components obtained from the serum samples from lung cancer patients or healthy controls were analyzed by differential proteomics analysis.CRP-bound serum amyloid A(CRP-SAA) was evaluated by coimmunoprecipitation(IP).Serum samples from two independent cohorts with lung cancer(retrospective cohort,242patients;prospective cohort,222 patients) and healthy controls(159 subjects) were used to evaluate the prognostic value of CRP-SAA by enzyme-linked immunosorbent assay.Results:CRP-SAA was identified specifically in serum samples from lung cancer patients by proteomic analysis.CRP binding to SAA was confirmed by co-IP in serum samples from lung cancer patients and cell culture media.The level of CRP-SAA was significantly higher in patients than in healthy controls(0.37 ± 0.58 vs.0.03 ± 0.04,P < 0.001).Elevated CRP-SAA levels were significantly associated with severe clinical features of lung cancer.The elevation of CRPSAA was associated with lower survival rates for both the retrospective(hazard ration[HR]= 2.181,95%confidence interval[CI]= 1.641-2.897,P < 0.001) and the prospective cohorts(HR = 2.744,95%CI = 1.810-4.161,P < 0.001).Multivariate Cox analysis showed that CRP-SAA was an independent prognostic marker for lung cancer.Remarkably,in stages l-ll patients,only CRP-SAA,not total SAA or CRP,showed significant association with overall survival in two cohorts.Moreover,univariate and multivariate Cox analyses also showed that only CRP-SAA could be used as an independent prognostic marker for early-stage lung cancer patients.Conclusion:CRP-SAA could be a better prognostic marker for lung cancer than total SAA or CRP,especially in earlystage patients. | Xue-Yan Zhang Ge Zhang Ying Jiang Dan Liu Man-Zhi Li Qian Zhong Shan-Qi Zeng Wan-Li Liu Mu-Sheng Zeng | 2015 | Chinese Journal of Cancer2015，34，8： | 10 |
| 8 | Development of the personalized criteria for microscopic review following four different series of hematology analyzer in a Chinese large scale hospital显示文摘 | CUI Wei WU Wei WANG Xin WANG Geng HAO Ying-ying CHEN Yu LUO Dan SHOU Wei-ling ZHANG Shuo XIANG Xue-fang SI Yong-zhen CHEN Qian CAI Hao LI Tan SHEN Han SHANG Kun ZHANG Yong-qiang | 2010 | Chinese Medical Journal2010，，22： | 10 |
| 9 | Improved design of special boundary elements for T-shaped reinforced concrete walls显示文摘This study examines the design provisions of the Chinese GB 50011-2010 code for seismic design of buildings for the special boundary elements of T-shaped reinforced concrete walls and proposes an improved design method.Comparison of the design provisions of the GB 50011-2010 code and those of the American code ACI 318-14 indicates a possible deficiency in the T-shaped wall design provisions in GB 50011-2010.A case study of a typical T-shaped wall designed in accordance with GB 50011-2010 also indicates the insufficient extent of the boundary element at the non-flange end and overly conservative design of the flange end boundary element.Improved designs for special boundary elements of T-shaped walls are developed using a displacement-based method.The proposed design formulas produce a longer boundary element at the non-flange end and a shorter boundary element at the flange end, relative to those of the GB 50011-2010 provisions.Extensive numerical analysis indicates that T-shaped walls designed using the proposed formulas develop inelastic drift of 0.01 for both cases of the flange in compression and in tension. | Ji Xiaodong Liu Dan Qian Jiaru | 2017 | Earthquake Engineering and Engineering Vibration2017，16，1： | 8 |
| 10 | Tumor microenvironments self-activated nanoscale metal-organic frameworks for ferroptosis based cancer chemodynamic/photothermal/chemo therapy显示文摘Ferroptosis,as a newly discovered cell death form,has become an attractive target for precision cancer therapy.Several ferroptosis therapy strategies based on nanotechnology have been reported by either increasing intracellular iron levels or by inhibition of glutathione(GSH)-dependent lipid hydroperoxidase glutathione peroxidase 4(GPX4).However,the strategy by simultaneous iron delivery and GPX4 inhibition has rarely been reported.Herein,novel tumor microenvironments(TME)-activated metal-organic frameworks involving Fe&Cu ions bridged by disulfide bonds with PEGylation(FCSP MOFs)were developed,which would be degraded specifically under the redox TME,simultaneously achieving GSH-depletion induced GPX4 inactivation and releasing Fe ions to produce ROS via Fenton reaction,therefore causing ferroptosis.More ROS could be generated by the acceleration of Fenton reaction due to the released Cu ions and the intrinsic photothermal capability of FCSP MOFs.The overexpressed GSH and H2O2 in TME could ensure the specific TME self-activated therapy.Better tumor therapeutic efficiency could be achieved by doxorubicin(DOX)loading since it can not only cause apoptosis,but also indirectly produce H2O2 to amplify Fenton reaction.Remarkable anti-tumor effect of obtained FCSP@DOX MOFs was verified via both in vitro and in vivo assays. | Yu Liang Li Zhang Chao Peng Shiyu Zhang Siwen Chen Xin Qian Wanxian Luo Qing Dan Yongyan Ren Yingjia Li Bingxia Zhao | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 7 |
| 11 | Molecular insights into the heterogeneity of telomere reprogramming in induced pluripotent stem cells显示文摘有各种各样的长度的 telomeres 的返老还童在导致的 pluripotent 干细胞(iPSCs ) 被发现了。telomere 长度规定的机制在 iPSCs 的正式就职和增长期间留下逃犯。我们证明 telomere 动力学在 reprogramming 和经过期间在鼠标 iPSCs 是可变的,并且建议这些差别多半源于多重潜在的因素,包括包括外长的 reprogramming 因素的表示的 telomerase 机械, telomerase 独立的机制和同种细胞的影响。用一个基因模型 telomerase 缺乏(为 iPSCs 的推导和段落的 Terc −/− 和 Terc +/−) 房间,我们发现 telomerase 在 iPSCs 的 reprogramming 和自强起一个关键作用。进一步,当由再结合的延伸和维护的其他的小径也被要求时, telomeres 的 telomerase 维护为真 pluripotency 的正式就职是必要的,然而并非足够。一起, telomere 生物学的几个方面可以在 iPSCs 说明可变 telomere 动力学。尤其是,采用在 iPSC reprogramming 期间维持 telomeres 的机制很类似于胚胎的干细胞的那些。这些调查结果可以也联系到这些机制能为在由体的房间的原子 reprogramming 以后的 telomere 异质负责的克隆的地原子转移。 | Fang Wang Yu Yin Xiaoying Ye Kai Liu Haiying Zhu Lingling Wang Maria Chiourea Maja Okuka Guangzhen Ji Jiameng Dan Bingfeng Zuo Minshu Li Qian Zhang Na Liu Lingyi Chen Xinghua Pan Sarantis Gagos David L Keefe Lin Liu | 2012 | Cell Research2012，22，4： | 7 |
| 12 | Garciesculenxanthone B induces PINK1-Parkin-mediated mitophagy and prevents ischemia-reperfusion brain injury in mice显示文摘Mitophagy is a selective form of autophagy involving the removal of damaged mitochondria via the autophagy-lysosome pathway.PINK1-Parkin-mediated mitophagy is one of the most important mechanisms in cardiovascular disease,cerebral ischemia-reperfusion(I/R)injury,and neurodegenerative diseases.In this study we conducted an image-based screening in YFP-Parkin HeLa cells to discover new mitophagy regulators from natural xanthone compounds.We found that garciesculenxanthone B(GeB),a new xanthone compound from Garcinia esculenta,induced the formation of YFP-Parkin puncta,a well known mitophagy marker.Furthermore,treatment with GeB dose-dependently promoted the degradation of mitochondrial proteins Tom20,Tim23,and MFN1 in YFP-Parkin HeLa cells and SH-SY5Y cells.We revealed that GeB stabilized PINK1 and triggered Parkin translocation to the impaired mitochondria to induce mitophagy,and these effects were abolished by knockdown of PINK1.Finally,in vivo experiments demonstrated that GeB partially rescued ischemia-reperfusion-induced brain injury in mice.Taken together,our findings demonstrate that the natural compound GeB can promote the PINK1-Parkin-mediated mitophagy pathway,which may be implicated in protection against I/R brain injury. | Man Wu Guang Lu Yuan-zhi Lao Hong Zhang Dan Zheng Zhao-qing Zheng Juan Yi Qian Xiang Li-ming Wang Hong-sheng Tan Hua Zhou Han-ming Shen Hong-xi Xu | 2021 | Acta Pharmacologica Sinica2021，42，2： | 7 |
| 13 | Adverse Effects of Exposure to Fine Particulate Matters and Ozone on Gestational Hypertension显示文摘Gestational hypertension(GH)is a common complication during pregnancy.GH is regarded as a potential public health challenge for pregnant women and infants.Limited evidence has linked ambient air pollution to an increased GH risk.However,most of the studies were conducted in developed countries,with inconsistent results obtained.The present study was performed to explore whether exposure to particulate matters with an aerodynamic diameter≤2.5(PM2.5)and ozone(O3)was related to elevated odds of GH in a Chinese population.This population-based cohort study involved 38115 pregnant women in Wuhan,China.All information was collected from the Wuhan Maternal and Child Health Management Information System,using standardized quality control.The daily air pollutant data for PM2.5 and O3 were obtained from the 20 monitoring stations of the Wuhan Environmental Monitoring Center during 2014.The nearest monitor approach was applied to individual exposure assessment of PM2.5 and O3 for each participant.After adjusting for major confounders and other air pollutants,a 10μg/m^3 increase in PM25 and O3 concentrations was found to correlate to a 1.14-fold[95%confidence interval(95%CI):1.09,1.20]and a 1.05-fold(95%CI:1.02,1.07)increase in GH risk,respectively.Additionally,stronger relationships between GH risk and PM25 and O3 exposure were observed in women who conceived in winter and summer,respectively.These findings suggest that air pollutants may contribute to the development of GH. | Rong YANG Dan LUO Yi-ming ZHANG Ke HU Zheng-min QIAN Li-qin HU Long-jiao SHEN Hong XIAN Juliet Iwelunmor Su-rong MEI | 2019 | Current Medical Science2019，39，6： | 7 |
| 14 | Aberrant promoter CpG methylation and its translational applications in breast cancer显示文摘Breast cancer is a complex disease driven by multiple factors including both genetic and epigenetic alterations.Recent studies revealed that abnormal gene expression induced by epigenetic changes,including aberrant promoter methylation and histone modification,plays a critical role in human breast carcinogenesis.Silencing of tumor suppressor genes(TSGs) by promoter CpG methylation facilitates cells growth and survival advantages and further results in tumor initiation and progression,thus directly contributing to breast tumorigenesis.Usually,aberrant promoter methylation of TSGs,which can be reversed by pharmacological reagents,occurs at the early stage of tumorigenesis and therefore may serve as a potential tumor marker for early diagnosis and therapeutic targeting of breast cancer.In this review,we summarize the epigenetic changes of multiple TSGs involved in breast pathogenesis and their potential clinical applications as tumor markers for early detection and treatment of breast cancer. | Ting-Xiu Xiang Ying Yuan Li-Li Li Zhao-Hui Wang Liang-Ying Dan Yan Chen Guo-Sheng Ren Qian Tao | 2013 | Chinese Journal of Cancer2013，32，1： | 7 |
| 15 | Central Obesity and Metabolic Risk Factors in Middle-aged Chinese显示文摘Objective Central obesity is considered to be a central component of metabolic syndrome. Waist circumference(WC) has been widely used as a simple indicator of central obesity. This study is aimed to evaluate the sensitivity of WC cut-off values for predicting metabolic risk factors in middle-aged Chinese. Methods The study involved 923 subjects aged 40-65 years. The metabolic risk factors were defined according to the Chinese Joint Committee for Developing Chinese Guidelines on Prevention and Treatment of Dyslipidemia in Adults. WC cut-off 85-90 cm and ≥90 cm were used as cut-off values of central pre-obesity and central obesity in males, respectively, while WC 80-85 cm and ≥85 cm were used as cut-off values of central pre-obesity and central obesity in females. Results First, WC values corresponding to body mass index(BMI) 24 kg/m2 and visceral fat area(VFA) 80 cm2 were 88.55 cm and 88.51 cm in males, and 81.46 cm and 82.51 cm in females respectively. Second, receiver operating characteristic curves showed that the optimal WC cut-off of value was 88.75 cm in males, higher than that in females(81.75 cm). Third, the subjects with higher WC values were more likely to have accumulating metabolic risk factors. The prevalence of metabolic risk factors increased linearly and significantly in relation to WC levels. Conclusion WC cut-off values of central pre-/central obesity are optimal to predict multiple metabolic risk factors. | YIN Xue Yao ZHENG Fen Ping ZHOU Jia Qiang DU Ying PAN Qian Qian ZHANG Sai Fei YU Dan LI Hong | 2014 | Biomedical and Environmental Sciences2014，27，5： | 6 |
| 16 | Food Frequency Questionnaire for Chinese Children Aged 12-17 Years: Validity and Reliability显示文摘Objective The primary objective of this study was to examine the validity and reliability of a semi-quantitative food frequency questionnaire(FFQ) among Chinese children aged 12-17 years. Methods A semi-quantitative 72-food item FFQ was developed for children aged 12-17 years. The reliability and validity of this FFQ were evaluated against 24-h dietary recalls(24 h DRs) to measure the consumption of foods and nutrients. We administered two FFQs and three DRs to children(N = 160) over a period of 1 month to evaluate the reliability and validity. Reliability was examined by quartile agreement and intraclass correlation coefficients(ICCs), and validity was examined by quartile agreement, Bland-Altman plots and correlation with DRs. Results For reliability, the ICCs between the two FFQs ranged from 0.21 to 0.76 for foods and nutrients, and the quartile agreement ranged from 70.0% to 95.0% in the same or adjacent quartiles. Spearman’s correlation coefficients of foods and nutrients between the second FFQ and the 24 h DRs ranged from-0.04 to 0.59. The Bland-Altman plots demonstrated good agreement across the range of intakes among nutrients. The quartile agreement ranged from 50.0% to 100.0%, with infrequent misclassification. Conclusion The FFQ assessment of dietary intakes demonstrated acceptable relative validity and high reproducibility for Chinese children aged 12-17 years. | LIU Dan JU La Hong YANG Zhen Yu ZHANG Qian GAO Jian Fen GONG Di Ping GUO Dan Dan LUO Shu Quan ZHAO Wen Hua | 2019 | Biomedical and Environmental Sciences2019，32，7： | 6 |
| 17 | Toxicity of Graphene Quantum Dots in Zebrafish Embryo显示文摘Objective To evaluate the bio-safety of graphene quantum dots(GQDs), we studied its effects on the embryonic development of zebrafish. Methods In vivo, biodistribution and the developmental toxicity of GQDs were investigated in embryonic zebrafish at exposure concentrations ranging from 12.5-200 μg/m L for 4-96 h post-fertilization(hpf). The mortality, hatch rate, malformation, heart rate, GQDs uptake, spontaneous movement, and larval behavior were examined. Results The fluorescence of GQDs was mainly localized in the intestines and heart. As the exposure concentration increased, the hatch and heart rate decreased, accompanied by an increase in mortality. Exposure to a high level of GQDs(200 μg/mL) resulted in various embryonic malformations including pericardial edema, vitelline cyst, bent spine, and bent tail. The spontaneous movement significantly decreased after exposure to GQDs at concentrations of 50, 100, and 200 μg/mL. The larval behavior testing(visible light test) showed that the total swimming distance and speed decreased dose-dependently. Embryos exposed to 12.5 μg/mL showed hyperactivity while exposure to higher concentrations(25, 50, 100, and 200 μg/mL) caused remarkable hypoactivity in the light-dark test. Conclusion Low concentrations of GQDs were relatively non-toxic. However, GQDs disrupt the progression of embryonic development at concentrations exceeding 50 μg/mL. | WANG Zhen Guo ZHOU Rong JIANG Dan SONG Jing E XU Qian SI Jing CHEN Yun Ping ZHOU Xin GAN Lu LI Jian Zhen ZHANG Hong LIU Bin | 2015 | Biomedical and Environmental Sciences2015，28，5： | 6 |
| 18 | Variation of Sweet Chemicals in Different Ripening Stages of Wolfberry Fruits显示文摘Objective To evaluate the variation of sweet chemicals(sugars and betaine) in fruits of six species in genus Lycium L. i.e. Lycium truncatum, L. cylindricum, L. dasystemum, L. dasystemum var. rubricaulium, L. chinense, and L. barbarum harvested at three different ripening stages. Methods A simple and effective method based on UPLC-ELSD was developed for the simultaneous determination of two monosaccharides(glucose and fructose), two disaccharides(sucrose and maltose), one sugar alcohol(xylitol), and betaine in wolfberry fruits(goji berries) of genus Lycium L. Results The six species of wolfberry fruits harvested at the three different ripening stages were evaluated in sugars and betaine contents. Fructose and glucose were the predominant sugars in mature wolfberry fruits. Fructose, glucose, and betaine, as well as total sugar contents, increased continually over the ripening process. L. truncatum and L. dasystemum had higher contents of sugars and betaine than the other species. Conclusion UPLC-ELSD is a simple, reliable and effective method for analysis of the sweet chemicals in wolfberries. Wolfberry fruits at the different ripening stages were significant different in sweetness. L. truncatum and L. dasystemum could be the potential sources of wolfberry fruit sources. | Dan Qian Jian Yang Li-ping Kang Rui-feng Ji Lu-qi Huang | 2017 | Chinese Herbal Medicines2017，9，4： | 6 |
| 19 | KLF4 initiates sustained YAP activation to promote renal fibrosis in mice after ischemia-reperfusion kidney injury显示文摘Acute renal injury(AKI)causes a long-term risk for progressing into chronic kidney disease(CKD)and interstitial fibrosis.Yes-associated protein(YAP),a key transcriptional cofactor in Hippo signaling pathway,shuttles between the cytoplasm and nucleus,which is required for the renal tubular epithelial cells repair in the acute phase of AKI.In this study we investigated the role of YAP during ischemia-reperfusion(IR)-induced AKI to CKD.Mice were subjected to left kidney IR followed by removal of the right kidney on the day before tissue harvests.Mouse shRNA expression adenovirus(Ad-shYAP or Ad-shKLF4)and mouse KLF4 expression adenovirus(Ad-KLF4)were delivered to mice by intrarenal injection on D7 after IR.We showed that the expression and nucleus distribution of YAP were persistently increased until the end of experiment(D21 after IR).The sustained activation of YAP in post-acute phase of AKI was accompanied by renal dysfunction and interstitial fibrosis.Knockdown of YAP significantly attenuated IR-induced renal dysfunction and decreased the expression of fibrogenic factors TGF-βand CTGF in the kidney.We showed that the expression of the transcription factor KLF4,lined on the upstream of YAP,was also persistently increased.Knockdown on KLF4 attenuated YAP increase and nuclear translocation as well as renal functional deterioration and interstitial fibrosis in IR mice,whereas KLF4 overexpression caused opposite effects.KLF4 increased the expression of ITCH,and ITCH facilitated YAP nuclear translocation via degrading LATS1.Furthermore,we demonstrated in primary cultured renal tubular cells that KLF4 bound to the promoter region of YAP and positively regulates YAP expression.In biopsy sample from CKD patients,we also observed increased expression and nuclear distribution of YAP.In conclusion,the activation of YAP in the post-acute phase of AKI is implicated in renal functional deterioration and fibrosis although it exhibits beneficial effect in acute phase.Reprogramming factor KLF4 is responsible for the persistent activation of YAP.Blocking the activation of KLF4-YAP pathway might be a way to prevent the transition of AKI into CKD. | Dan Xu Pan-pan Chen Pei-qing Zheng Fan Yin Qian Cheng Zhuan-li Zhou Hong-yan Xie Jing-yao Li Jia-yun Ni Yan-zhe Wang Si-jia Chen Li Zhou Xiao-xia Wang Jun Liu Wei Zhang Li-min Lu | 2021 | Acta Pharmacologica Sinica2021，42，3： | 6 |
| 20 | Hyperthyroid heart disease in pregnancy: Retrospective analysis of a case series and review of the literature显示文摘BACKGROUND Hyperthyroidism in pregnancy may pose a great threat to maternal and fetal health.The risk of hyperthyroid heart disease(HHD),even heart failure,is significantly elevated in pregnant women.AIM To investigate the clinical characteristics,prognosis,and therapy of HHD in pregnant women.METHODS We searched the patient registry data at West China Second University Hospital of Sichuan University in Chengdu,China,following the approval by the Ethics Committee.We retrospectively analyzed the clinical characteristics of pregnant women diagnosed with HHD.The medical records of women with HHD during pregnancy from January 2012 to December 2017 were obtained from the electronic medical records system.All the included patients were followed in outpatient clinics and by telephone interviews until October 2018.RESULTS A total of 155 patients were diagnosed with thyrotoxicosis,of whom six were diagnosed with HHD.Three of them had regular antenatal care.Two patients were complicated with acute heart failure attacks,and one of them had a stillbirth.Both of these patients had a long history of Graves’disease with poor treatment compliance.Treatments of precipitating factors such as the control of infection could relieve the symptoms and prolong gestation for a better prognosis.Hyperthyroid heart failure could be controlled with aggressive diuretics and management of the coexisting complications.Intense monitoring and timely anti-heart failure treatment were crucial in patients with severe cardiac damage.Our findings indicated the importance of regular antenatal care and treatment adherence in patients with hyperthyroidism.CONCLUSION The timely and accurate diagnosis of HHD and the implementation of effective management are important for a better prognosis in pregnant women with HHD.Improvement in patients’awareness of thyrotoxicosis is needed. | Dan Shan Yi Bai Qiu-He Chen Yu-Xia Wu Qian Chen Ya-Yi Hu | 2019 | World Journal of Clinical Cases2019，7，19： | 5 |
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