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35篇 您的检索式：作者名="David Archer"

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	题名	作者	年代	出处	被引量
1	黑曲霉菌为宿主菌的重组表达性质粒的构建显示文摘目的建立以pyrG基因为选择标志 ,以黑曲霉菌为宿主菌的蛋白质表达系统。方法以PCR扩增获得的黑曲霉菌 pyrG基因的部分序列片段为探针作两次噬菌斑原位杂交 ,从黑曲霉菌ATCC 12 0 49基因文库中克隆获得长度为 9.8kb的DNA片段 ,将其中含 pyrG基因的 2 .3kb片段亚克隆至表达性载体PIGF中 ,获得重组质粒。对克隆基因进行了全基因测定和分析。以黑曲霉菌ATCC 12 0 49的 pyrG缺陷株M 5 4为受体菌 ,用聚乙二醇 /氯化钙方法作基因转化实验 ,将重组质粒导入该受体菌并在其中表达。结果从黑曲霉菌ATCC 12 0 49基因文库中克隆获得了pyrG基因 ,构建了含该基因的重组表达性载体 pYG1.2。序列分析表明该基因与黑曲霉菌L112的 pyrG基因编码序列的同源性为 93 .9% ,两者经推导的氨基酸的同源性为 98.9%。重组质粒 pYG1.2可使黑曲霉菌ATCC 12 0 49的 pyrG缺陷株M5 4发生基因转化 ,成为Pyr+ 。结论在克隆了 pyrG基因的基础上成功构建了以 pyrG基因为选择标志。	刘钟滨 David J Jeenes David B Archer	2001	同济大学学报(医学版)2001，22，3：	4
2	ACCF/AHA 2009 Expert Consensus Document on Pulmonary Hypertension显示文摘	Vallerie V. McLaughlin Stephen L. Archer David B. Badesch Robyn J. Barst Harrison W. Farber Jonathan R. Lindner Michael A. Mathier Michael D. McGoon Myung H. Park Robert S. Rosenson Lewis J. Rubin Victor F. Tapson John Varga	2009	Journal of the American College of Cardiology2009，，17：	2
3	ACCF/AHA 2009 Expert Consensus Document on Pulmonary Hypertension: A Report of the American College of Cardiology Foundation Task Force on Expert Consensus Documents and the American Heart Association显示文摘	Vallerie V. McLaughlin Stephen L. Archer David B. Badesch Robyn J. Barst Harrison W. Farber Jonathan R. Lindner Michael A. Mathier Michael D. McGoon Myung H. Park Robert S. Rosenson Lewis J. Rubin Victor F. Tapson John Varga	2009	Circulation2009，，16：	1
4	The risk ranking of projects : a methodology 显示文摘	DAVID Baccarini RICHARD Archer	2001	International Journal of Project Management2001，19，：	1
5	Biochar: A Synthesis of Its Agronomic Impact beyond Carbon Sequestration显示文摘	Spokas Kurt A Cantrell Keri B M Novak Jeffrey Archer David W Ippolito James A Collins Harold P Boateng Akwasi A Lima Isabel M Lamb Marshall C McAloon Andrew J Lentz Rodrick D Nichols Kristine A	2012	Journal of Environmental Quality2012，，4：	1
6	Influence of Sowing Date on Emergence Characteristics of Maize Seed Coated with a Temperature-activated Polymer显示文摘	Gesch Russ W Archer David W	2005	Agronomy Journal2005，97，6：	1
7	Hollow micro-/nanostructures: synthesis and applications 显示文摘	Lou Xiong Wen( David) Lynden A Archer Yang Zichao	2008	Adv Mate2008，20，21：	1
8	Bile Duct Injury During Laparoscopic Cholecystectomy: Results of a National Survey显示文摘	Stephen B. Archer David W. Brown C. Daniel Smith Gene D. Branum John G. Hunter	2001	Annals of Surgery2001，，4：	1
9	The Risk Ranking of Projects:A Thodology显示文摘	David Baccarini Richard Archer	2001	International Journal of Project Management2001，19，：	1
10	The risk ranking of projects:a methodology显示文摘	David Baccarini Richard Archer	2001	International Journal of Project Management2001，，19：	1
11	Functional MRI interactions between dysplastic nodules and overlying cortex in periventricular nodular heterotopia显示文摘	John S. Archer David F. Abbott Richard A.J. Masterton Susan M. Palmer Graeme D. Jackson	2010	Epilepsy and Behavior2010，，4：	1
12	The risk ranking of proj-ects:a methodology 显示文摘	David Baccarini and Richard Archer	2001	International Journal of Project Manage-ment2001，19，：	1
13	Postmenopausal skin and estrogen显示文摘	David F. Archer	2012	Gynecological Endocrinology2012，，2：	1
14	Visceral artery aneurysm rupture显示文摘	Sandra C. Carr David M. Mahvi John R. Hoch Charles W. Archer William D. Turnipseed	2001	Journal of Vascular Surgery2001，，4：	1
15	The risk ranking of projects:a methodology显示文摘	David Baccarini Richard Archer	2001	International Journal of Project Management2001，，19：	1
16	Bile Duct Injury During Laparoscopic Cholecystectomy: Results of a National Survey显示文摘	Stephen B. Archer David W. Brown C. Daniel Smith Gene D. Branum John G. Hunter	2001	Annals of Surgery2001，，4：	1
17	Broad dissemination of plasmid - mediated quinolone resistance genes in sediments of two urban coastal wet- lands 显示文摘	David E Cummings Karisa F Archer David J Arriola	2011	Environmental Science & Technology2011，45，2：	1
18	Bil Duct Injury During Laparoscopic cholcystectomy results of a national survey显示文摘	Sephen B Archer MD David W	2001	Ann Surg2001，234，4：	1
19	Model based experimental performance analysis of a microscale LiBr-H20 steam-driven doub- le-effect absorption chiller 显示文摘	Hongxi Yin Ming Qu David H Archer	2010	Applied Thermal Engineering2010，30，：	1
20	Progress Report of a Phase I Study of the Intracerebral Microinfusion of a Recombinant Chimeric Protein Composed of Transforming Growth Factor (TGF)-α and a Mutated form of the Pseudomonas Exotoxin Termed PE-38 (TP-38) for the Treatment of Malignant Brain显示文摘	John H. Sampson Gamal Akabani Gary E. Archer Darell D. Bigner Mitchel S. Berger Allan H. Friedman Henry S. Friedman James E. Herndon II Sandeep Kunwar Steve Marcus Roger E. McLendon Alison Paolino Kara Penne James Provenzale Jennifer Quinn David A. Reardo	2003	Journal of Neuro - Oncology2003，，1：	1

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