|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Surface characteristics and wetting mechanism of respirable coal dust显示文摘In this study,FTIR and XPS spectroscopy,specific surface area and pore size analyses,wetting and other experimental means have been applied to investigate respirable coal dust surface properties and wetting mechanism.The research shows that coal dust surface structures,chemical and element compositions are changed in the refining process of coal dust.Compared with large particle coal dust,respirable coal dust is a weak hydrophilic and strong hydrophobic substance.The reason:the smaller the particle size of coal dust,the more unsaturated its surface.The absorption of air is strong and it is easy to form an air film on coal dust surfaces. Coal dust contact with water is actually a compound contact of liquid in contact with a solid and gas in contact with a solid. The smaller the particle size,the larger the pore volume and the higher the degree of surface roughness,the larger the area of gas in contact with a solid and the worse the coal dust wettability.Another reason is that the changes in the composition of atoms on the dust surface and structures affect coal dust wettability.The results of this study establish a theoretical basis for the invention of effective reduced-dust technology. | YANG Jing WU Xiukun GAO Jianguang LI Gaiping | 2010 | Mining Science and Technology2010，20，3： | 25 |
| 2 | In vitro and in vivo activity of D-serine in combination with β-lactam antibiotics against methicillin-resistant Staphylococcus aureus显示文摘As D-amino acids play important roles in the physiological metabolism of bacteria,combination of D-amino acids with antibiotics may provide synergistic antibacterial activity. The aim of the study was to evaluate in vitro and in vivo activity of D-serine alone and in combination withβ-lactams against methicillin-resistant Staphylococcus aureus(MRSA) strains, and to explore the possible sensitization mechanisms. The activity of D-serine, β-lactams alone and in combinations was evaluated both in vitro by standard MICs, time–kill curves and checkerboard assays, and in vivo by murine systemic infection model as well as neutropenic thigh infection model. An in vitro synergistic effect was demonstrated with the combination of D-serine and β-lactams against MRSA standard and clinical strains.Importantly, the combinations enhanced the therapeutic efficacy in the animal models as compared toβ-lactam alone groups. Initial mechanism study suggested possible revision of D-alanine-D-alanine residue to D-alanine-D-serine in peptidoglycan by adding of D-alanine in the medium, which may cause decreased affinity to PBPs during transpeptidation. In conclusion, D-serine had synergistic activity in combination with β-lactams against MRSA strains both in vitro and in vivo. Considering the relatively good safety of D-serine alone or in combination with β-lactams, D-serine is worth following up as new anti-MRSA infection strategies. | Qing Wang Yuemeng Lv Jing Pang Xue Li Xi Lu Xiukun Wang Xinxin Hu Tongying Nie Xinyi Yang Yan Q.Xiong Jiandong Jiang Congran Li Xuefu You | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 6 |
| 3 | Efficient generation of the mouse model with a defined point mutation through haploid cell-mediated gene editing显示文摘Generation of mouse models carrying a defined point mutation,especially disease-related point mutations,is of considerable interest for research in biology and medicine.The standard method based on embryonic stem cell(ESC)-mediated homologous recombination(HR)is time-and labor-consuming.CRISPR-Cas9 system has been rapidly developed recently and adopted for efficient gene-editing in different species(Mei et al.,2016).Remarkably。 | Leixin Wei Xiukun Wang Suming Yang Wen Yuan Jinsong Li | 2017 | Journal of Genetics and Genomics2017，44，9： | 6 |
| 4 | Genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii from Beijing,China显示文摘The objective of this study was to investigate the genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii clinical isolates from Beijing,China.173 A.baumannii clinical isolates from hospitals in Beijing from 2006 to 2009 were first subjected to high level aminoglycoside resistance(HLAR,MIC to gentamicin and amikacin>512 mg/mL)phenotype selection by broth microdilution method.The strains were then subjected to genetic basis analysis by PCR detection of the aminoglycoside modifying enzyme genes(aac(3)-Ⅰ,aac(3)-Ⅱc,aac(60)-Ⅰb,aac(60)-Ⅱ,aph(4)-Ⅰa,aph(30)-Ⅰ,aph(30)-Ⅱb,aph(30)-Ⅲa,aph(30)-Ⅵa,aph(2″)-Ⅰb,aph(2″)-Ⅰc,aph(2″)-Ⅰd,ant(2″)-Ⅰa,ant(3″)-Ⅰand ant(40)-Ⅰa)and the 16S rRNA methylase genes(armA,rmtB and rmtC).Correlation analysis between the presence of aminoglycoside resistance gene and HLAR phenotype were performed by SPSS.Totally 102(58.96%)HLAR isolates were selected.The HLAR rates for year 2006,2007,2008 and 2009 were 52.63%,65.22%,51.11%and 70.83%,respectively.Five modifying enzyme genes(aac(3)-Ⅰ,detection rate of 65.69%;aac(60)-Ⅰb,detection rate of 45.10%;aph(30)-Ⅰ,detection rate of 47.06%;aph(30)-Ⅱb,detection rate of 0.98%;ant(3″)-Ⅰ,detection rate of 95.10%)and one methylase gene(armA,detection rate of 98.04%)were detected in the 102 A.baumannii with aac(3)-Ⅰ+aac(60)-Ⅰ+þant(3″)-Ⅰ+armA(detection rate of 25.49%),aac(3)-Ⅰ+aph(30)-Ⅰ+ant(3″)-Ⅰ+armA(detection rate of 21.57%)and ant(3″)-Ⅰ+armA(detection rate of 12.75%)being the most prevalent gene profiles.The values of chi-square tests showed correlation of armA,ant(3″)-Ⅰ,aac(3)-Ⅰ,aph(30)-Ⅰand aac(60)-Ⅰb with HLAR.armA had significant correlation(contingency coefficient 0.685)and good contingency with HLAR(kappa 0.940).The high rates of HLAR may cause a serious problem for combination therapy of aminoglycoside with β-lactams against A.baumannii infections.As armA was reported to be able to cause high level aminoglycoside resistance to most of the clinical important aminoglycosides(gentamicin,amikacin,tobramycin,etc),the function of aminoglycoside modifying enzyme gene(s)in A.baumannii carrying armA deserves further investigation. | Lu Nie Yuemeng Lv Min Yuan Xinxin Hu Tongying Nie Xinyi Yang Guoqing Li Jing Pang Jingpu Zhang Congran Li Xiukun Wang Xuefu You | 2014 | Acta Pharmaceutica Sinica B2014，4，4： | 3 |
| 5 | NIR Chemical-Image Analysis Based on Adaptive Local Optimization PSO显示文摘Principal component analysis(PCA) combined with cluster analysis has become an effective approach for Near-infrared(NIR) chemical image analysis.Traditional cluster algorithms are sensitive to initial starting conditions and can be trapped into local optimal solutions. To overcome the drawbacks, we develop a new algorithm in this paper which improves Particle swarm optimization with Adaptive local optimization(ALO-PSO).Simulation experiments performed on NIR image of tablet verify the feasibility and effectiveness of the proposed algorithm. Experimental results of the clustering performances indicate that ALO-PSO algorithm offers an alternative approach for solving data clustering problems in NIR chemical image analysis. | YANG Xiukun ZHONG Mingliang JING Xiaojun WANG Jianli CHEN Xuqi | 2014 | Chinese Journal of Electronics2014，23，1： | 2 |
| 6 | New method for discretization of continuous attributes in rough set theory显示文摘A new method for discretization of continuous attributes is put forward to overcome the limitation of the traditional rough sets,which cannot deal with continuous attributes.The method is based on an improved algorithm to produce candidate cut points and an algorithm of reduction based on variable precision rough information entropy.With the guarantee of consistency of decision system,the method can reduce the number of cut points and improve efficiency of reduction.Adopting variable precision rough information entropy as measure criterion,it has a good tolerance to noise.Experiments show that the algorithm yields satisfying reduction results. | Rong Cong Xiukun Wang Kai Li Nanhai Yang | 2010 | Journal of Systems Engineering and Electronics2010，21，2： | 2 |
| 7 | An efficient papaya leaf distortion mosaic potyvirus vector for virus-induced gene silencing in papaya显示文摘Papaya(Carica papaya L.)is regarded as an excellent model for genomic studies of tropical trees because of its short generation time and its small genome that has been sequenced.However,functional genomic studies in papaya depend on laborious genetic transformations because no rapid tools exist for this species.Here,we developed a highly efficient virus-induced gene silencing(VIGS)vector for use in papaya by modifying an artificially attenuated infectious clone of papaya leaf distortion mosaic virus(PLDMV;genus:Potyvirus),PLDMV-E,into a stable Nimble Cloning(NC)-based PLDMV vector,pPLDMV-NC,in Escherichia coli.The target fragments for gene silencing can easily be cloned into pPLDMV-NC without multiple digestion and ligation steps.Using this PLDMV VIGS system,we silenced and characterized five endogenous genes in papaya,including two common VIGS marker genes,namely,phytoene desaturase,Mg-chelatase H subunit,putative GIBBERELLIN(GA)-INSENSITIVE DWARF1A and 1B encoding GA receptors;and the cytochrome P450 gene CYP83B1,which encodes a key enzyme involved in benzylglucosinolate biosynthesis.The results demonstrate that our newly developed PLDMV VIGS vector is a rapid and convenient tool for functional genomic studies in papaya. | Decai Tuo Pu Yan Guangyuan Zhao Hongguang Cui Guopeng Zhu Yang Liu Xiukun Yang He Wang Xiaoying Li Wentao Shen Peng Zhou | 2021 | Horticulture Research2021，8，1： | 2 |
| 8 | Polymyxin resistance caused by large-scale genomic inversion due to IS26 intramolecular translocation in Klebsiella pneumoniae显示文摘Polymyxin B and polymyxin E(colistin)are presently considered the last line of defense against human infections caused by multidrug-resistant Gram-negative organisms such as carbapenemase-producer Enterobacterales,Acinetobacter baumannii,and Klebsiella pneumoniae.Yet resistance to this last-line drugs is a major public health threat and is rapidly increasing.Polymyxin S2(S2)is a polymyxin B analogue previously synthesized in our institute with obviously high antibacterial activity and lower toxicity than polymyxin B and colistin.To predict the possible resistant mechanism of S2for wide clinical application,we experimentally induced bacterial resistant mutants and studied the preliminary resistance mechanisms.Mut-S,a resistant mutant of K.pneumoniae ATCC BAA-2146(Kpn2146)induced by S2,was analyzed by whole genome sequencing,transcriptomics,mass spectrometry and complementation experiment.Surprisingly,large-scale genomic inversion(LSGI)of approximately 1.1 Mbp in the chromosome caused by IS26mediated intramolecular transposition was found in Mut-S,which led to mgrB truncation,lipid A modification and hence S2resistance.The resistance can be complemented by plasmid carrying intact mgrB.The same mechanism was also found in polymyxin B and colistin induced drug-resistant mutants of Kpn2146(Mut-B and Mut-E,respectively).This is the first report of polymyxin resistance caused by IS26 intramolecular transposition mediated mgrB truncation in chromosome in K.pneumoniae.The findings broaden our scope of knowledge for polymyxin resistance and enriched our understanding of how bacteria can manage to survive in the presence of antibiotics. | Haibin Li Lang Sun Han Qiao Zongti Sun Penghe Wang Chunyang Xie Xinxin Hu Tongying Nie Xinyi Yang Guoqing Li Youwen Zhang Xiukun Wang Zhuorong Li Jiandong Jiang Congran Li Xuefu You | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 1 |
| 9 | Acoustic signal analysis of underwater elastic cylinder显示文摘The echoes of underwater elastic cylinder comprise two types of acoustic components: Geometrical scattering waves and elastic scattering waves. The transfer function is appropriate to characterize the echo of targets. And the discrete wavelet transform of amplitude spectrum is presented and used to identify the resonant components of underwater targets. | LI Xiukun,YANG Shi’e (Department of Underwater Acoustic Engineering, Harbin Engineering University Harbin 150001) | 2001 | Chinese Journal of Acoustics2001，20，1： | 1 |
| 10 | Ridge Distance Estimation in Fingerprint Images:Algorithm and Performance Evaluation显示文摘 | Jie Tian Xiukun Yang | 2004 | EURASIP Journal on Applied Signal Processing2004，2，4： | 1 |
| 11 | Extraction of Echo Characteristics of Underwater Target Based on Cepstrum Method显示文摘The analysis and characteristic extraction of target echo characteristics are important in underwater target detection and recognition. Rigid acoustic scattering components are generally used as major echo contributors with relatively stable characteristic information. Previous studies focus on echo characteristics from a single angle, thereby limiting the amount of extracted characteristic information. This paper aims to establish a full-angle rigid echo components model and overcome the difficulty of the extraction of time delay characteristics of narrow-band acoustic scattering echoes. On the basis of the analysis of the target echo highlight model, the echo characteristics of rigid acoustic scattering components are extracted in the cepstrum domain, and a wavelet process is proposed to enhance the effect of time delay estimation. Experimental data indicate that the extracted time delay characteristics accord with the rigid echo characteristics of underwater target, thereby validating the effectiveness of the cepstrum method. | Hongjian Jia Xiukun Li Xiangxia Meng Yang Yang | 2017 | Journal of Marine Science and Application2017，16，2： | 1 |
| 12 | Characteristic Analysis of Underwater Acoustic Scattering Echoes in the Wavelet Transform Domain显示文摘在水下的声学的散布回应时间空间组织并且及时是 aliasing 并且频率领域。当事件角度是未知的时,回响性质的不同系列没被识别。这篇文章在 monostatic 声纳的目标回响调查变化处理这个问题。有类似的结构的母亲小浪用匹配的过滤器,和在延期因素之间的理论表达式根据预处理信号波形被建议了,事件角度在小浪域被导出。在这个方法能有效地分开几何散布的免费地的水池表演的模拟数据和试验性的结果的分析目标回响的部件。在一个单个角度从几何回响获得的时间延期评价与一致指向几何特征,没有角度信息,它为目标识别提供一个基础。调查结果为分析在实际海洋环境散布回响的橡皮提供珍贵卓见。 | Mei Yang Xiukun Li Yang Yang Xiangxia Meng | 2017 | Journal of Marine Science and Application2017，16，1： | 1 |
| 13 | Blind Source Separation based on Time-Frequency Morphological Characteristics for Rigid Acoustic Scattering by Underwater Objects显示文摘僵硬声学的散布由的部件的分离在水下目标在获得如此的目标的结构的特征是必要的。克服看起来有一样的僵硬结构的问题光谱在时间领域的结构,时间频率窗帘来源分离(BSS ) 能与图象形态学在联合被使用分开不同目标的僵硬散布部件。基于一个热点模型,有时间频率分发的目标的僵硬散布结构的分离被推出。用一个词法过滤器,在 Wigner-Ville 分布(WVD ) 的不同特征观察到术语能被简化移开任何跨术语的干扰单个汽车术语和十字。由选择术语表明的汽车的时间和频率点, BSS 的精确性能被改进。试验性的模拟被使用了,与在播送信号,相对振幅和时间延期参数的脉搏宽度的变化,以便分析这个新方法的可行性。模拟结果证明新方法不仅能分开僵硬散布当有弹性的散布并且僵硬散布时,部件,而且罐头也分开部件同时存在。试验性的结果证实新方法能在分开僵硬散布结构被使用在水下目标。 | Yang Yang Xiukun Li | 2016 | Journal of Marine Science and Application2016，15，2： | 1 |
| 14 | A Security Defense Method Against Eavesdroppers in the Communication-Based Train Control System显示文摘The communication-based train control system is the safety guarantee for automatic train driving.Wireless communication brings network security risks to the communication-based train control system.The eavesdropping of the transmitted information by unauthorized third-party personnel will lead to the leakage of the estimated value of the system state,which will lead to major accidents.This paper focuses on solving the problem of defense against eavesdropping threats and proposes an eavesdropping defense architecture.This defense architecture includes a coding mechanism based on punishing eavesdroppers,an information upload trigger mechanism based on contribution,and a random information transmission strategy,and provides a guarantee for the privacy protection of information.This research makes three contributions.First,it is the first attempt to construct an information encoding mechanism with punishing eavesdroppers as the objective function;Second,for the first time,an information upload trigger mechanism based on contribution is proposed;Third,the strategy of random transmission of information is proposed.The proposed method in this paper is verified by taking the medium and low-speed maglev train as the object.The experimental results show that,compared with Gaussian noise and non-Gaussian noise mechanisms,the coding mechanism proposed in this paper can not only protect the security of information but also make the estimation error of eavesdroppers tend to be infinite.Using the state estimation error as a metric,the average growth rate of the state estimation error of the system using the trigger mechanism in this paper is less than 2%while improving the security of the system.The transmission strategy in this paper does not increase the system state estimation error while improving the security of the system. | YANG Li WEI Xiukun WEN Chenglin | 2023 | Chinese Journal of Electronics2023，32，5： | 0 |
| 15 | Detecting malignant patients via modified boosted tree显示文摘As one of the most effective measures to extract useful information from medical database and provide scientific decision-making for diagnosis and treatment of diseases, medical data mining has become an increasingly hot topic in the last few years. Some of the intrinsic characteristics of medical databases, such as the huge volume and imbalanced samples as well as stringent performance standards, make this mining process particularly challenging. By elaborating various challenges existing in Task 1 of KDD Cup 2008 competition, this paper analyzes some potential solutions to these problems and presents a modified boosted tree as the final classification model. This model ranked the fourth among all the solutions to Task 1. We hope that our analysis and solutions to these challenges would contribute to the development of medical data mining applications. | DONG CaiLing YIN YiLong YANG XiuKun | 2010 | Science China(Information Sciences)2010，53，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员