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| 1 | Expression, characterization, and antimicrobial ability of T4 lysozyme from methylotrophic yeast Hansenula polymorpha A16显示文摘Lysozyme is an enzyme that is essential for protection against bacterial infections.In this study,a T4 lysozyme gene was cloned into the yeast expression vector pPIC9K under the control of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase promoter (pGAP).A Hansenula polymorpha-derived ribosomal DNA (rDNA)-targeting element was inserted into the expression vector and was critical for stable DNA integration into the H.polymorpha chromosome.Recombinant T4 lysozyme was successfully expressed in the yeast H.polymorpha A16;0.49 g L-1 secreted recombinant T4 lysozyme was obtained 72 h after incubation in culture broth that had an initial pH of 6.0.Recombinant T4 lysozyme showed lytic activity against the cell walls of the gram positive bacteria,Micrococcus lysodeikticus,and the gram negative bacteria Xanthomonas campestris pv.malvacearum and Xanthomonas oryzae pv.oryzae.The zone of inhibition assay was used to evaluate antimicrobial activity.Mass spectrometry showed the N-terminal sequence of recombinant T4 lysozyme was identical to that of the native enzyme.SDS-PAGE indicated that the molecular mass of recombinant T4 lysozyme was 18.7 kD which corresponds to a monomer of the native enzyme.SDS-PAGE without 0.2 mol L-1 dithiothreitol treatment detected two bands (15 and 31 kD) suggesting that some recombinant T4 lysozyme formed interand intra-molecular disulfide bonds which resulted in loss of enzyme activity. | WANG Nan WANG YueJu LI GangQiang SUN Ning LIU DeHu | 2011 | Science China(Life Sciences)2011，54，6： | 3 |
| 2 | High-fidelity and clean nanotransfer lithography using structure-embedded and electrostatic-adhesive carriers显示文摘Metallic nanostructures are becoming increasingly important for both fundamental research and practical devices.Many emerging applications employing metallic nanostructures often involve unconventional substrates that are flexible or nonplanar,making direct lithographic fabrication very difficult.An alternative approach is to transfer prefabricated structures from a conventional substrate;however,it is still challenging to maintain high fidelity and a high yield in the transfer process.In this paper,we propose a high-fidelity,clean nanotransfer lithography method that addresses the above challenges by employing a polyvinyl acetate(PVA)film as the transferring carrier and promoting electrostatic adhesion through triboelectric charging.The PVA film embeds the transferred metallic nanostructures and maintains their spacing with a remarkably low variation of<1%.When separating the PVA film from the donor substrate,electrostatic charges are generated due to triboelectric charging and facilitate adhesion to the receiver substrate,resulting in a high large-area transfer yield of up to 99.93%.We successfully transferred the metallic structures of a variety of materials(Au,Cu,Pd,etc.)with different geometries with a<50-nm spacing,high aspect ratio(>2),and complex 3D structures.Moreover,the thin and flexible carrier film enables transfer on highly curved surfaces,such as a single-mode optical fiber with a curvature radius of 62.5μm.With this strategy,we demonstrate the transfer of metallic nanostructures for a compact spectrometer with Cu nanogratings transferred on a convex lens and for surface-enhanced Raman spectroscopy(SERS)characterization on graphene with reliable responsiveness. | Zhuofei Gan Jingxuan Cai Zhao Sun Liyang Chen Chuying Sun Junyi Yu Zeyu Liang Siyi Min Fei Han Yu Liu Xing Cheng Shuhui Yu Dehu Cui Wen-Di Li | 2023 | Microsystems & Nanoengineering2023，9，1： | 1 |
| 3 | Expression, Characterization and Antimicrobial Ability of a Variant T4 Lysozyme in Pichia pastoris显示文摘T4 lysozyme was engineered with disulfide bonds and expressed in Pichia pastoris. The secreted proteins were purified and made into powder by lyophilization. Recombinant protein purity was more than 70% measured by HPLC. The lytic activity of variant T4-lysozyme was measured by the lysis of the cell wall of Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, Ralstonia solanacearum comb.nov, Clavibacter michiganensis subsp. michiganensis, X. campestris pv. malvacearum, Fusarium oxysporium sp. vasinfectum, Verticillium dahliae kleb. Inhibition zone assay showed that variant T4 lysozyme significantly inhibited X. o. oryzicola and X. c. malvacearum. The antifungal activities of this protein against F. o. vasinfectum and V. d. kleb were also analyzed. | Ning SUN Sanfeng CHEN Xiangming XIE Yueju WANG Gangqiang LI Nan WANG Dehu LIU | 2014 | Agricultural Science & Technology2014，15，3： | 0 |
| 4 | The effects of cement density and pipe dimension on casing waves in oil wells显示文摘当时,一个 cased 洞里的声学的地通过由联合实验测量的数字建模被学习第一并且 scond 接口被结合很好。casing 到达(ACA ) 的振幅上的钢管子的水泥,直径和厚度的密度的效果被调查,并且数字结果的部分与试验性的结果相比。这些结果证明 ACA 与水泥的增加的密度减少。在那里存在为低密度、正常密度的水泥的 ACA 之间的大差别。因此,不同标准应该与不同密度为水泥在结合的评估被拿。当当它的直径作为一个常数保留时,钢管子的厚度增加, casing 波浪的到达时间仍然保持不变,当 ACA 增加时。但是当有经常的厚度的管子的直径增加时,波浪被推迟的 casing 的到达时间,和 ACA 减少。至于通常在油矿使用的三种钢管子, casing 到达的相对振幅在大管子中是更大的。另外,总体上,有水泥的密度的 casing 到达的相对振幅的变化趋势的数字结果与试验性的一致。 | CHEN Dehu WANG Xiuming ZHANG Hailan WANG Dong WEI Tao LIU Liping | 2011 | Chinese Journal of Acoustics2011，30，1： | 0 |
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