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| 1 | G protein-coupled receptor 183 facilitates endothelial-to- hematopoietic transition via Notch1 inhibition显示文摘 | Panpan Zhang Qiuping He Dongbo Chen Weixiao Liu Lu Wang Chunxia Zhang Dongyuan Ma Wei Li Bing Liu Feng Liu | 2015 | Cell Research2015，25，10： | 7 |
| 2 | Genome Editing and Its Applications in Model Organisms显示文摘Technological advances are important for innovative biological research. Development of molecular tools for DNA manipulation, such as zinc finger nucleases(ZFNs), transcription activator-like effector nucleases(TALENs), and the clustered regularly-interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas), has revolutionized genome editing. These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases. In the last few years, substantial progress has been made in CRISPR/Cas technology, including technical improvements and wide application in many model systems. This review describes recent advancements in genome editing with a particular focus on CRISPR/Cas, covering the underlying principles, technological optimization, and its application in zebrafish and other model organisms, disease modeling, and gene therapy used for personalized medicine. | Dongyuan Ma Feng Liu | 2015 | Genomics, Proteomics & Bioinformatics2015，13，6： | 4 |
| 3 | Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics显示文摘Limited knowledge of cellular and molecular mechanisms underlying hematopoietic stem cell and multipotent progenitor(HSC/MPP)expansion within their native niche has impeded the application of stem cell-based therapies for hematological malignancies.Here,we constructed a spatiotemporal transcriptome map of mouse fetal liver(FL)as a platform for hypothesis generation and subsequent experimental validation of novel regulatory mechanisms.Single-cell transcriptomics revealed three transcriptionally heterogeneous HSC/MPP subsets,among which a CD93-enriched subset exhibited enhanced stem cell properties.Moreover,by employing integrative analysis of single-cell and spatial transcriptomics,we identified novel HSC/MPP‘pocket-like’units(HSC PLUS),composed of niche cells(hepatoblasts,stromal cells,endothelial cells,and macrophages)and enriched with growth factors.Unexpectedly,macrophages showed an 11-fold enrichment in the HSC PLUS.Functionally,macrophage-HSC/MPP co-culture assay and candidate molecule testing,respectively,validated the supportive role of macrophages and growth factors(MDK,PTN,and IGFBP5)in HSC/MPP expansion.Finally,cross-species analysis and functional validation showed conserved cell-cell interactions and expansion mechanisms but divergent transcriptome signatures between mouse and human FL HSCs/MPPs.Taken together,these results provide an essential resource for understanding HSC/MPP development in FL,and novel insight into functional HSC/MPP expansion ex vivo. | Suwei Gao Qiang Shi Yifan Zhang Guixian Liang Zhixin Kang Baofeng Huang Dongyuan Ma Lu Wang Jianwei Jiao Xiangdong Fang Cheng-Ran Xu Longqi Liu Xun Xu Berthold Gottgens Cheng Li Feng Liu | 2022 | Cell Research2022，32，1： | 4 |
| 4 | Efficient conversion of benzene and syngas to toluene and xylene over ZnO-ZrO_(2)&H-ZSM-5 bifunctional catalysts显示文摘A series of ZnO-ZrO_(2) solid solutions with different Zn contents were synthesized by the urea coprecipitation method,which were coupled with H-ZSM-5 zeolite to form bifunctional catalysts.As a new benzene alkylation reagent,syngas was used instead of methanol to realize the efficient conversion of syngas and benzene into toluene and xylene.A suitable ratio of ZnO-ZrO_(2) led to the significant improvement in the catalytic performance,and a suitable amount of acid helped to increase the selectivity of toluene/xylene and reduce the selectivity of the by-products ethylbenzene and C^(9+) aromatics.The highest benzene conversion of 89.2%and toluene/xylene selectivity of 88.7%were achieved over 10%ZnO-ZrO_(2)&H-ZSM-5(Si/Al=23)at a pressure of 3 MPa and a temperature of 450℃.In addition,the effect of the zeolite framework structure on product distribution was examined.Similar to the molecular dynamics of aromatic hydrocarbons,H-ZSM-5 zeolites comprise 10-membered-ring pores,which are beneficial to the activation of benzene;hence,the conversion of benzene is higher.H-ZSM-35 and HMOR zeolites exhibited small eight-membered-ring channels,which were not conducive to the passage of benzene;hence,the by-product ethylbenzene exhibits a higher selectivity.The distance between the active centers of the bifunctional catalysts was the main factor affecting the catalytic performance,and the powder mixing method was more conducive to the conversion of syngas and benzene. | Xiao Zhao Xuan Shi Zhongshun Chen Long Xu Chengyi Dai Yazhou Zhang Xinwen Guo Dongyuan Yang Xiaoxun Ma | 2022 | Chinese Journal of Chemical Engineering2022，35，5： | 2 |
| 5 | Synthesis of core–shell structured zeolite-A@mesoporous silica composites for butyraldehyde adsorption显示文摘 | Haijun Yu Yingying Lv Kuoyan Ma Changguo Wang Zhaoteng Xue Yujuan Zhao Yonghui Deng Ya Dai Dongyuan Zhao | 2014 | Journal of Colloid And Interface Science2014，，： | 1 |
| 6 | The rising zebrafish research in China:Meeting report of the 3rd Chinese Zebrafish Principal Investigator Meeting & the Inaugural Meeting of China Zebrafish Society显示文摘The zebrafish has become a very important animal model,not only for developmental biology and genetics,but also for disease modeling and drug discovery.Since the year of 2000,zebrafish research has been rapidly growing in China,and the laboratories using zebrafish as the main subject have been increasing dramatically(Xie et al.,2015).For example,the number of participants | Dongyuan Ma Yuanyuan Xue Yifan Zhang Yonghua Sun Anming Meng Feng Liu | 2016 | Journal of Genetics and Genomics2016，43，10： | 1 |
| 7 | Comparison of viscoelasticity between normal human sciatic nerve and amniotic membrane显示文摘Sciatic nerve tissue was obtained from the gluteus maximus muscle segment of normal human cadavers and amniotic membrane tissue was obtained from healthy human puerperant placentas.Both tissues were analyzed for their stress relaxation and creep properties to determine suitability for transplantation applications.Human amniotic membrane and sciatic nerve tissues had similar tendencies for stress relaxation and creep properties.The stress value of the amniotic membrane stress relaxation group decreased to a greater extent compared with the sciatic nerve stress relaxation group.Similarly,the stress value of the amniotic membrane creep group increased to a greater extent compared with the sciatic nerve creep group.The stress relaxation curve for human amniotic membrane and sciatic nerve showed a logarithm correlation,while the creep curve showed an exponential correlation.These data indicate that amniotic membrane tissue has better stress relaxation and creep properties compared with sciatic nerve tissue. | Donghui Xu Conghai Zhao Huili Ma Jun Wei Dongyuan Li | 2013 | Neural Regeneration Research2013，8，14： | 1 |
| 8 | Model- ing water infiltration in a large layered soil column with a modified Green-Ampt model and HYDRUS-1D 显示文摘 | Ma Ying Feng Shaoyuan Su Dongyuan | 2010 | Computers and Electronics in Agriculture2010，，： | 1 |
| 9 | Modeling water infiltration in a large layered soil column with a modified Green-Ampt model and HYDRUS-1D显示文摘 | Ma Ying Feng Shaoyuan Su Dongyuan | 2010 | Computers and Electronics in Agriculture2010，71，1： | 1 |
| 10 | 血管微环境通过Klf6a-ccl25b通路调控造血干/祖细胞驻留和扩增显示文摘文章简介微环境是造血干细胞多步骤、多阶段发育过程中不可或缺的因素。目前对于造血干细胞产生及维持的研究较多,但人们对于造血干细胞扩增的机制仍知之甚少。本研究充分利用斑马鱼体外发育和早期胚胎透明的优势,通过激光共聚焦显微镜实时观察,发现尾部造血组织处的造血干细胞毗邻于血管内皮细胞,并且其迁移和扩增具有尾部静脉特异的方向性。 | Yuanyuan Xue Junhua Lv Chunxia Zhang Lu Wang Dongyuan Ma 刘峰 | 2018 | 科学新闻2018，0，4： | 0 |
| 11 | Activation of lineage competence in hemogenic endothelium precedes the formation of hematopoietic stem cell heterogeneity显示文摘Hematopoietic stem and progenitor cells (HSPCs) are considered as a heterogeneous population, but precisely when, where and how HSPC heterogeneity arises remain largely unclear. Here, using a combination of single-cell multi-omics, lineage tracing and functional assays, we show that embryonic HSPCs originate from heterogeneous hemogenic endothelial cells (HECs) during zebrafish embryogenesis. Integrated single-cell transcriptome and chromatin accessibility analysis demonstrates transcriptional heterogeneity and regulatory programs that prime lymphoid/myeloid fates at the HEC level. Importantly, spi^(2+) HECs give rise to lymphoid/myeloid-primed HSPCs (L/M-HSPCs) and display a stress-responsive function under acute inflammation. Moreover, we uncover that Spi2 is required for the formation of L/M-HSPCs through tightly controlling the endothelial-to-hematopoietic transition program. Finally, single-cell transcriptional comparison of zebrafish and human HECs and human induced pluripotent stem cell-based hematopoietic differentiation results support the evolutionary conservation of L/M-HECs and a conserved role of SPI1 (spi2 homolog in mammals) in humans. These results unveil the lineage origin, biological function and molecular determinant of HSPC heterogeneity and lay the foundation for new strategies for induction of transplantable lineage-primed HSPCs in vitro. | Jun Xia Mengyao Liu Caiying Zhu Shicheng Liu Lanlan Ai Dongyuan Ma Ping Zhu Lu Wang Feng Liu | 2023 | Cell Research2023，33，6： | 0 |
| 12 | Nucleic acids induced peptide-based AIE nanoparticles for fast cell imaging显示文摘Herein,we utilized nucleic acids induced peptide co-assembly strategy to develop novel nucleic acids induced peptide-based AIE(NIP-AIE)nanoparticles.Strong fluorescent of AIE could be observed when a little amount of nucleic acids was added into the peptide solution,and the intensity could be regulated by the concentration of nucleic acids.This AIE nanoparticle with good biocompatibility could achieve fast cell imaging.It is also proved that the fluorescence intensity of AIE decreased with time,which indicates that the reducible cross-linkers of Wpc peptide by GSH and nanoparticles gradually disintegrate in cell.Based on the different of AIE fluorescence signals which regulated by the formation and disintegration of nanoparticles,this AIE system is expected to be used for real-time monitoring of drug release from peptide-based nano carriers in vivo or in vitro,and may provide a new platform for the construction of other organic AIE nanoparticles. | Wenjun Li Yaping Zhang Yanchao Wang Yue Ma Dongyuan Wang Heng Li Xiyang Ye Feng Yin Zigang Li | 2021 | Chinese Chemical Letters2021，32，4： | 0 |
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