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| 1 | First determination of the highly genotoxic fungal contaminant altertoxin II in a naturally infested apple sample显示文摘Altertoxin II is an epoxide-holding perylene quinone and secondary metabolite produced by Alternaria molds.Its genotoxic potential and the high concentrations determined in laboratory fungal cultures resulted in an increased scientific interest in this emerging contaminant.However,no natural occurrence in food has been reported to date to the best of our knowledge.Here,we unambiguously identified altertoxin II in a naturally infected,unprocessed apple.Applying a validated LC-MS/MS method,fruit peel spots(approx.1 mm in diameter),typically associated with Alternaria infestation,were tested for 17 mycotoxins produced by this genus.Negligible concentrations of tentoxin were determined 24 h after harvest,and the dibenzopyrones alternariol,alternariol monomethyl ether(AME)and AME-3-sulfate after six months of storage.Overall,these contamination patterns can be considered unlikely to be of toxicological concern for consumers.However,an inconspicuous peel lesion(0.5×1 cm)separately sampled from a stored apple,exhibited considerably high concentrations of 14 Alternaria toxins,including dibenzopyrones,their barely studied modified forms(glucosides and sulfates),as well as genotoxic perylene quinones including altertoxin II.Considering the toxicological potential of this compound,contaminations in such infected fruit parts should not be underestimated,e.g.in the case of exposure through the consumption of fresh smoothies or fruit juices. | Hannes Puntscher Doris Marko Benedikt Warth | 2020 | Emerging Contaminants2020，6，1： | 2 |
| 2 | Expression of interleukin-4 and interleukin-13 and their receptors in colorectal cancer显示文摘 | Andrea Formentini Philipp Braun Harald Fricke Karl-Heinrich Link Doris Henne-Bruns Marko Kornmann | 2012 | International Journal of Colorectal Disease2012，，10： | 2 |
| 3 | Response prediction in metastasised colorectal cancer using intratumoural thymidylate synthase: Results of a randomised multicentre trial显示文摘 | Marko Kornmann Holger Hebart Kathleen Danenberg Roland Goeb Ludger Staib Martina Kron Doris Henne-Bruns Peter Danenberg Karl-Heinrich Link | 2011 | European Journal of Cancer2011，，10： | 1 |
| 4 | Appendix Carcinoid Associated With The Peutz-Jeghers Syndrome显示文摘 | Sabine Hofmann Thomas F.E. Barth Marko Kornmann Doris Henne-Bruns | 2014 | International Journal of Surgery Case Reports2014，，： | 1 |
| 5 | Induction of antioxidative Nrf2 gene transcription by coffee in humans: depending on genotype?显示文摘 | Ute Boettler Nadine Volz Nicole Teller Larisa Haupt Tamara Bakuradze Gerhard Eisenbrand Gerhard Bytof Ingo Lantz Lyn Griffiths Doris Marko | 2012 | Molecular Biology Reports2012，，6： | 1 |
| 6 | Long-Term Results of 2 Adjuvant Trials Reveal Differences in Chemosensitivity and the Pattern of Metastases Between Colon Cancer and Rectal Cancer显示文摘 | Marko Kornmann Ludger Staib Thomas Wiegel Martina Kron Doris Henne-Bruns Karl-Heinrich Link Andrea Formentini | 2012 | Clinical Colorectal Cancer2012，，： | 1 |
| 7 | Urotensin II levels in patients with inflammatory bowel disease显示文摘BACKGROUND Patients with inflammatory bowel disease(IBD)are associated with increased cardiovascular risk and have increased overall cardiovascular burden.On the other hand,urotensin II(UII)is one of the most potent vascular constrictors with immunomodulatory effect that is connected with a number of different cardiometabolic disorders as well.Furthermore,patients with ulcerative colitis have shown increased expression of urotensin II receptor in comparison to healthy controls.Since the features of IBD includes chronic inflammation and endothelial dysfunction as well,it is plausible to assume that there is connection between increased cardiac risk in IBD and UII.AIM To determine serum UII levels in patients with IBD and to compare them to control subjects,as well as investigate possible associations with relevant clinical and biochemical parameters.METHODS This cross sectional study consecutively enrolled 50 adult IBD patients(26 with Crohn’s disease and 24 with ulcerative colitis)and 50 age and gender matched controls.Clinical assessment was performed by the same experienced gastroenterologist according to the latest guidelines.Ulcerative Colitis Endoscopic Index of Severity and Simple Endoscopic Score for Crohn’s Disease were used for endoscopic evaluation.Serum levels of UII were determined using the enzyme immunoassay kit for human UII,according to the manufacturer’s instructions.RESULTS IBD patients have significantly higher concentrations of UII when compared to control subjects(7.57±1.41 vs 1.98±0.69 ng/mL,P<0.001),while there were no significant differences between Crohn’s disease and ulcerative colitis patients(7.49±1.42 vs 7.65±1.41 ng/mL,P=0.689).There was a significant positive correlation between serum UII levels and high sensitivity C reactive peptide levels(r=0.491,P<0.001)and a significant negative correlation between serum UII levels and total proteins(r=-0.306,P=0.032).Additionally,there was a significant positive correlation between serum UII levels with both systolic(r=0.387,P=0.005)and diastolic(r=0.352,P=0.012)blood pressure.Moreover,serum UII levels had a significant positive correlation with Ulcerative Colitis Endoscopic Index of Severity(r=0.425,P=0.048)and Simple Endoscopic Score for Crohn’s Disease(r=0.466,P=0.028)scores.Multiple linear regression analysis showed that serum UII levels retained significant association with high sensitivity C reactive peptide(β±standard error,0.262±0.076,P<0.001)and systolic blood pressure(0.040±0.017,P=0.030).CONCLUSION It is possible that UII is involved in the complex pathophysiology of cardiovascular complications in IBD patients,and its purpose should be investigated in further studies. | Damir Alicic Dinko Martinovic Doris Rusic Piero Marin Zivkovic Ivana Tadin Hadjina Marino Vilovic Marko Kumric Daria Tokic Daniela Supe-Domic Slaven Lupi-Ferandin Josko Bozic | 2021 | World Journal of Gastroenterology2021，27，36： | 0 |
| 8 | Growth differentiation factor-15 serum concentrations reflect disease severity and anemia in patients with inflammatory bowel disease显示文摘BACKGROUND Population of patients with inflammatory bowel disease(IBD)is burdened by various extraintestinal manifestations which substantially contribute to greater morbidity and mortality.Growth-differentiation factor-15(GDF-15)is often overexpressed under stress conditions,such as inflammation,malignancies,heart failure,myocardial ischemia,and many others.AIM To explore the association between GDF-15 and IBD as serum concentrations of GDF-15 were shown to be an independent predictor of poor outcomes in multiple diseases.An additional aim was to determine possible associations between GDF-15 and multiple clinical,anthropometric and laboratory parameters in patients with IBD.METHODS This cross-sectional study included 90 adult patients diagnosed with IBD,encompassing both Crohn’s disease(CD)and ulcerative colitis(UC),and 67 healthy age-and sex-matched controls.All patients underwent an extensive workup,including colonoscopy with subsequent histopathological analysis.Disease activity was assessed by two independent gastroenterology consultants specialized in IBD,employing well-established clinical and endoscopic scoring systems.GDF-15 serum concentrations were determined following an overnight fasting,using electrochemiluminescence immunoassay.RESULTS In patients with IBD,serum GDF-15 concentrations were significantly higher in comparison to the healthy controls[800(512-1154)pg/mL vs 412(407-424)pg/mL,P<0.001],whereas no difference in GDF-15 was found between patients with CD and UC[807(554-1451)pg/mL vs 790(509-956)pg/mL,P=0.324].Moreover,multiple linear regression analysis showed that GDF-15 levels predict CD and UC severity independent of age,sex,and C-reactive protein levels(P=0.016 and P=0.049,respectively).Finally,an association between GDF-15 and indices of anemia was established.Specifically,negative correlations were found between GDF-15 and serum iron levels(r=-0.248,P=0.021),as well as GDF-15 and hemoglobin(r=-0.351,P=0.021).Accordingly,in comparison to IBD patients with normal hemoglobin levels,GDF-15 serum levels were higher in patients with anemia(1256(502-2100)pg/mL vs 444(412-795)pg/mL,P<0.001).CONCLUSION For the first time,we demonstrated that serum concentrations of GDF-15 are elevated in patients with IBD in comparison to healthy controls,and the results imply that GDF-15 might be involved in IBD pathophysiology.Yet,it remains elusive whether GDF-15 could serve as a prognostic indicator in these patients. | Ante Tonkic Marko Kumric Ivna Akrapovic Olic Doris Rusic Piero Marin Zivkovic Daniela Supe Domic Zeljko Sundov Ivan Males Josko Bozic | 2024 | World Journal of Gastroenterology2024，30，13： | 0 |
| 9 | N-acetyl cysteine alters the genotoxic and estrogenic properties of Alternaria toxins in naturally occurring mixtures显示文摘It is unclear if complex mycotoxin mixtures produced by Alternaria spp.act estrogenic and/or genotoxic under physiological conditions,particularly considering the co-occurrence with antioxidants in food.Thus,this study focused on enlightening the impact of N-acetyl cysteine(NAC),as a representative antioxidative SH-donor,on the mentioned toxicological endpoints of the signature Alternaria toxins alternariol(AOH),altertoxin-II(ATX-II)and a complex extract(CE)of an Alternaria alternata culture.Using Ishikawa cells as an in vitro model,we monitored alterations in toxin concentrations by LC-MS/MS,estrogenicity by alkaline phosphatase assays,cytotoxicity by sulforhodamine B assays,genotoxicity by single-cell gel electrophoresis and the transcription of selected genes of interest by quantitative realtime PCR.The results indicate that the strong genotoxic effects of epoxide-carrying perylene quinones such as ATX-II are erased in the presence of NAC.The cellular effects of ATX-II/AOH mixtures are dominated by the genotoxicity of the perylene chinone.In this mixture,AOH regained its estrogenicity when coincubated with NAC.In contrast,NAC treatment of an AOH/CE mixture did not result in a recovery of estrogenicity,but in potentiated anti-estrogenic effects.These findings were in line with gene transcription data,that indicated the aryl hydrocarbon receptor(AhR)to be a prime mediator of Alternaria toxin e induced antagonistic effects towards estrogen receptor signaling.Taken together,further studies on potential endocrine-disruptive properties of non-genotoxic perylene quinones should be a future research priority in the field of these emerging contaminants. | Georg Aichinger Dino Grgic Julia Beisl Francesco Crudo Benedikt Warth Elisabeth Varga Doris Marko | 2022 | Emerging Contaminants2022，8，1： | 0 |
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