| 1 | Atg7 is required for acrosome biogenesis during spermatogenesis in mice显示文摘acrosome 是盖住精子原子核的前面的部分并且在授精的过程起一个必要作用的一个专业化细胞器。位于这个 lysosome 相关的细胞器(LRO ) 的生物的续生说下面的分子的机制大部分仍然是未知的。这里,我们显示出房间特定的 Atg7 大美人老鼠是的那个细菌由于在 acrosome 生物的续生说的一个缺点不肥沃并且显示了类似于人的 globozoospermia 的显型;这个繁殖缺点被 intracytoplasmic 精子注射成功地救。而且,在细菌房间的 Atg7 的弄空没影响细菌房间的开发的早阶段,但是精子发生在以后上演,在 Golgi 期间没有通过到保险丝进一个单个 acrosomal 泡的 proacrosomal 泡分阶段执行,它最后导致了不规则或将近 round-headed 精子。Autophagic 流动在弄空 Atg7 的细菌房间被破坏,最后导致 LC3 变化形式的失败到 Golgi 导出仪器的泡。另外, Atg7 部分调整了另一 globozoospermia 相关的蛋白质,联系 Golgi 的 PDZ 卷和卷卷包含主题的蛋白质(GOPC ) ,在 acrosome 生物的续生说期间。最后,任何一个 autophagy 的注射或进睾丸的 lysosome 禁止者导致了类似的显型到细菌的房间特定的 Atg7 大美人老鼠。总的来说,我们的结果揭开为在 acrosome 的生物的续生说的 Atg7 的一个新角色,并且我们提供证据为 acrosome 支持 autolysosome 起源假设。 | Hongna Wang Haifeng Wan Xixia Li Weixiao Liu Qi Chen Yaqing Wang Lin Yang Hongmei Tang Xiujun Zhang Enkui Duan Xiaoyang Zhao Fei Gao Wei Li | 2014 | Cell Research2014,24,7: | 25 |
| 3 | Appropriate Metabolic Energy, Crude Protein, Calciumand Available Phosphorus Levels of Diet for BlackMuscovy Duck at 43-91 Days of Age显示文摘[Objective] The paper was to study the effects of diet formulation with different nutrition levels on growth and slaughter performance of black muscovy duck at the age of 43-91 days. [Method] Totally 288 individuals of 43-day-old black muscovy duck were randomly divided into nine treatments, four repeats each treatment and eight ducks each repeat. Using L9(34) orthogonal experiment design(four factors and three levels),three metabolic energy levels(12.32, 12.72, 13.12 MJ/kg), three crude protein levels(14%, 16%, 18%), three calcium levels(0.6%, 0.8%, 1.0%)and three available phosphorus levels(0.30%, 0.45%, 0.6%) were designed, and a total of nine feeding groups(C1-C9) were designed according to different nutritional levels. Through combination of feeding test(49 d) and metabolic test(pre-test 4 d and formal test 3 d), the growth performance,meat performance and apparent nutrient digestibility of ducks were determined. [Result] The ducks in experimental group C2 had the highest average daily gain, the lowest feed gain ratio, the largest body oblique length, chest width, chest depth and tibia length, which had significant or ex-tremely significant differences with other groups(P<0.05 or P<0.01). The slaughter rate, semi-eviscerated rate, eviscerated rate and meat quality of ducks in C2 group were higher(P<0.05). The apparent digestibility of crude protein and dietary metabolic energy in C2 group were 88.68% and63.07%, respectively, both were significantly or extremely significantly higher than the other groups( P<0.05 or P<0.01), while the interaction effect of calcium and phosphorus level on apparent digestibility was not obvious. [Conclusion] Under the experimental conditions, the suitable metabolic energy, crude protein, calcium, available phosphorus and total phosphorus levels of 43-91 days old black muscovy ducks were 12.32 MJ/kg,16.10%, 0.92%, 0.45% and 0.68%, respectively. | Zhang Ling Gu Wenjie Zhang Kai Duan Xiujun Qin Haorong Bian Youqing duan yongbo | 2018 | Animal Husbandry and Feed Science2018,10,4: | 0 |
| 4 | Incubation Effect on Eggs of Tibetan Chicken under Low Altitude Condition显示文摘[Objective] In order to study incubation conditions of Tibetan chickens in low altitude areas. [Method]Eggs of Tibetan chicken were transported to low altitude areas in highland areas for artificial hatching. [Results] The results showed that the average hatching rate of fertilized eggs was 85. 33%; the average healthy chick rate was 91. 77 %; the average primary body mass was 31. 21 g. Hatching rate of eggs in group 2 was the highest,which reached 90. 52%,the average egg mass was 43. 25 g and the average egg shape index was 1. 32. Hatching rate of eggs in group 2 was 13. 11% and 2. 47% higher than that in group 1 and group 3 respectively,which was 5. 19% higher than the average value. Hatching rate and healthy chick rate of eggs in group 2 also showed the same trend. [Conclusion] High hatching effect could be obtained under the better hatching condition in low altitude areas in highland areas. | Zhang Ling Jiang Chunmao Duan Xiujun Yang Xiaozhi Qin Haorong Liu Dan | 2017 | Animal Husbandry and Feed Science2017,9,4: | 0 |