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| 1 | Genetic diversity and construction of core collection in Chinese wheat genetic resources显示文摘Genetic diversity among 5029 accessions representing a proposed Chinese wheat core collection was analyzed using 78 pairs of fluorescent microsatellite (SSR) primers mapped to 21 chromosomes. A stepwise hierarchical sampling strategy with priority based on 4×105 SSR data-points was used to construct a core collection from the 23090 initial collections. The core collection consisted of 1160 accessions, including 762 landraces, 348 modern varieties and 50 introduced varieties. The core ac-counts for 23.1% of the 5029 candidate core accessions and 5% of the 23090 initial collections, but retains 94.9% of alleles from the candidate collections and captures 91.5% of the genetic variation in the initial collections. These data indicate that it is possible to maintain genetic diversity in a core collection while retaining fewer accessions than the accepted standard, i.e., 10% of the initial collections captured more than 70% of their genetic diversity. Estimated genetic representation of the core con-structed by preferred sampling (91.5%) is much higher than that by random sampling (79.8%). Both mean genetic richness and genetic diversity indices of the landraces were higher than those of the modern varieties in the core. Structure and principal coordinate analysis revealed that the landraces and the modern varieties were two relatively independent subpopulations. Strong genetic differentiation associated with ecological environments has occurred in the landraces, but was relatively weak in the modern cultivars. In addition, a mini-core collection was constructed, which consisted of 231 accessions with an estimated 70% representation of the genetic variation from the initial collections. The mini-core has been distributed to various research and breeding institutes for detailed phenotyping and breeding of genetic introgression lines. | HAO ChenYang DONG YuChen WANG LanFen YOU GuangXia ZHANG HongNa GE HongMei JIA JiZeng ZHANG XueYong | 2008 | Chinese Science Bulletin2008，53，10： | 27 |
| 2 | Atg7 is required for acrosome biogenesis during spermatogenesis in mice显示文摘acrosome 是盖住精子原子核的前面的部分并且在授精的过程起一个必要作用的一个专业化细胞器。位于这个 lysosome 相关的细胞器(LRO ) 的生物的续生说下面的分子的机制大部分仍然是未知的。这里,我们显示出房间特定的 Atg7 大美人老鼠是的那个细菌由于在 acrosome 生物的续生说的一个缺点不肥沃并且显示了类似于人的 globozoospermia 的显型;这个繁殖缺点被 intracytoplasmic 精子注射成功地救。而且,在细菌房间的 Atg7 的弄空没影响细菌房间的开发的早阶段,但是精子发生在以后上演,在 Golgi 期间没有通过到保险丝进一个单个 acrosomal 泡的 proacrosomal 泡分阶段执行,它最后导致了不规则或将近 round-headed 精子。Autophagic 流动在弄空 Atg7 的细菌房间被破坏,最后导致 LC3 变化形式的失败到 Golgi 导出仪器的泡。另外, Atg7 部分调整了另一 globozoospermia 相关的蛋白质,联系 Golgi 的 PDZ 卷和卷卷包含主题的蛋白质(GOPC ) ,在 acrosome 生物的续生说期间。最后,任何一个 autophagy 的注射或进睾丸的 lysosome 禁止者导致了类似的显型到细菌的房间特定的 Atg7 大美人老鼠。总的来说,我们的结果揭开为在 acrosome 的生物的续生说的 Atg7 的一个新角色,并且我们提供证据为 acrosome 支持 autolysosome 起源假设。 | Hongna Wang Haifeng Wan Xixia Li Weixiao Liu Qi Chen Yaqing Wang Lin Yang Hongmei Tang Xiujun Zhang Enkui Duan Xiaoyang Zhao Fei Gao Wei Li | 2014 | Cell Research2014，24，7： | 25 |
| 3 | Loss of YTHDF2-mediated m6A-dependent mRNA clearance facilitates hematopoietic stem cell regeneration显示文摘 | Hu Wang Hongna Zuo Jin Liu Fei Wen Yawei Gao Xudong Zhu Bo Liu Feng Xiao Wengong Wang Gang Huang Bin Shen Zhenyu JU | 2018 | Cell Research2018，28，10： | 16 |
| 4 | Analysis of coherent structures in drag-reducing polymer solution flow based on proper orthogonal decomposition显示文摘Direct numerical simulation(DNS) of forcing homogeneous isotropic turbulence with polymers was performed.In order to understand the polymers effect on turbulent coherent structures,proper orthogonal decomposition was performed to identify coherent structures based on DNS data,so as to analyze the remarkable difference due to the addition of polymers.The results showed that the numbers for eigenmodes required for capturing coherent structures were 32 and 24 for the Newtonian fluid and polymer solution flows,respectively,which means the decrease of the complexity in polymer solution flow.Through the POD energy spectrum,it was found that the turbulent kinetic energy is distributed onto a large number of eigenmodes whether in the Newtonian fluid flow or polymer solution flow,suggesting that polymer solution flow is still turbulent in one aspect.Besides,the POD eigenmodes were investigated,which found that the small-scale structures are inhibited in polymer solution flow. | CAI WeiHua LI FengChen ZHANG HongNa WANG Yue WANG Lu | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，5： | 5 |
| 5 | Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles显示文摘The extraction of nucleic acid is recognized as one of the most essential steps in molecular biology for initiating other downstream applications such as sequencing, amplification, hybridization, and cloning. Many commercial kits and methods are currently available that allow the extraction of only one type of nucleic acids-DNA or RNA. However, in parallel clinical detection of several diseases, a method for simultaneous extraction of both DNA and RNA from the same source is needed in such cases. In this study, a method for simultaneous extraction of DNA and RNA from bacteria based on magnetic nanoparticles(MNPs) was described. Lysis buffers were prepared to help the nucleic acid released and adsorbed to MNPs. Then, two washing buffers were used to remove the contamination of proteins and carbohydrates. The nucleic acids were finally eluted by Deoxyribonuclease(DNase) and Ribonucleases(RNase) free water. Different factors which might affect the purification of the nucleic acid were investigated, and the quantity and quality parameters of the nucleic acid were also recorded. The DNA and RNA extracted from bacteria were then respectively subjected to polymerase chain reaction(PCR) and reverse transcription PCR(RT-PCR) to further confirm its quality. The results indicated that our method can be successfully used to simultaneously extract DNA and RNA from bacteria. | Jiuhai Wang Zeeshan Ali Nianyue Wang Wenbiao Liang Hongna Liu Fu Li Haowen Yang Lei He Libo Nie Nongyue He Zhiyang Li | 2015 | Science China Chemistry2015，58，11： | 5 |
| 6 | Overexpressed Cyclin D1 and CDK4 proteins are responsible for the resistance to CDK4/6 inhibitor in breast cancer that can be reversed by PI3K/mTOR inhibitors显示文摘CDK4/6 inhibitors are the standard treatment in advanced HR+/HER2-breast cancer patients.Nevertheless,the resistance to CDK4/6 inhibitors is inevitable and the strategies to overcome resistance are of great interest.Here,we show that the palbociclibresistant breast cancer cells expressed significantly higher levels of Cyclin D1 and CDK4 proteins because of upregulated protein synthesis.Silencing Cyclin D1 or CDK4 led to cell cycle arrest while silencing Cyclin E1 or CDK2 restored the sensitivity to palbociclib.Furthermore,PI3K/mTOR pathway was hyper-activated in palbociclib-resistant cells,leading to more phosphorylated 4E-BP1 and higher levels of Cyclin D1 and CDK4 translation.Targeting PI3K/mTOR pathway with a specific PI3Kαinhibitor(BYL719)or an mTOR inhibitor(everolimus)reduced the protein levels of Cyclin D1 and CDK4,and restored the sensitivity to palbociclib.The tumor samples expressed significantly higher levels of Cyclin D1,CDK4,p-AKT and p-4E-BP1 after progression on palbociclib treatment.In conclusion,our findings suggest that overexpressed Cyclin D1 and CDK4 proteins lead to the resistance to CDK4/6 inhibitor and PI3K/mTOR inhibitors are able to restore the sensitivity to CDK4/6 inhibitors,which provides the biomarker and rationale for the combinational use of CDK4/6 inhibitors and PI3K/mTOR inhibitors after CDK4/6 inhibitor resistance in breast cancer. | Zijie Cai Jingru Wang Yudong Li Qianfeng Shi Liang Jin Shunying Li Mengdi Zhu Qi Wang Lok Lam Wong Wang Yang Hongna Lai Chang Gong Yandan Yao Yujie Liu Jun Zhang Herui Yao Qiang Liu | 2023 | Science China(Life Sciences)2023，66，1： | 4 |
| 7 | Pt nanoparticles entrapped in ordered mesoporous carbon for enantioselective hydrogenation显示文摘 | Bo Li Xiaohong Li Hongna Wang Peng Wu | 2011 | Journal of Molecular Catalysis A Chemical2011，，1： | 3 |
| 8 | Mesoscale SST perturbation-induced impacts on climatological precipitation in the Kuroshio-Oyashio extension region,as revealed by the WRF simulations显示文摘Mesoscale coupling between perturbations of mesoscale sea surface temperature (SST) and lowlevel winds has been extensively studied using available high-resolution satellite observations. However, the climatological impacts of mesoscale SST perturbations (SST meso ) on the free atmosphere have not been fully understood. In this study, the rectified eff ect of SSTmeso on local climatological precipitation in the Kuroshio- Oyashio Extension (KOE) region is investigated using the Weather Research and Forecasting (WRF) Model;two runs are performed, one forced by low-resolution SST fields (almost no mesoscale signals) and another by additional high-resolution SSTmeso fields extracted from satellite observations. Climatological precipitation response to SST meso is characterized mainly by enhanced precipitation on the warmer flank of three oceanic SST fronts in this region. The results show that the positive correlation between the 10-m wind speed perturbations and SSTmeso is well captured by the WRF model with a reasonable spatial pattern but relatively weak strength. The addition of SSTmeso improves the climatological precipitation simulated by WRF with a better representation of fine-scale structures compared with satellite observations. A closer examination on the underlying mechanism suggests that while the pressure adjustment mechanism can explain the climatological precipitation enhancement along the fronts and the relatively high contribution of the convective precipitation, other factors such as synoptic events should also be taken into consideration to account for the seasonality of the precipitation response. | GAO Jiaxiang ZHANG Rong-Hua WANG Hongna | 2019 | Journal of Oceanology and Limnology2019，37，2： | 3 |
| 9 | LIN9 confers paclitaxel resistance in triple negative breast cancer cells by upregulating CCSAP显示文摘LIN9 functions to regulate cell mitotic process.Dysregulation of LIN9 expression is associated with development of human cancers.In this study we assessed the association of LIN9 expression with paclitaxel resistance and clarified the underlying mechanisms for the first time.LIN9 expression in breast cancer tissues was retrieved from publicly available online databases and statistically analyzed.Human TNBC cell lines MDA-MB-231 and MDA-MB-468 and their corresponding paclitaxelresistant sublines 231PTX and 468PTX were used to assess the expression of LIN9 by qRT-PCR and Western blot,cell growth by cell counting,cell viability by MTS assay,and cell apoptosis by flow cytometry.The data showed that high LIN9 expression in breast cancer patients receiving chemotherapy was related to poor overall survival(OS).LIN9 expression was upregulated in paclitaxel-resistant TNBC cells compared to their parental cells.Knockdown of LIN9 or treatment of paclitaxel-resistant TNBC cells with a bromo-and extra-terminal domain inhibitor(BETi)JQ1 which also decreased LIN9 expression enhanced the sensitivity of paclitaxel-resistant TNBC cells to paclitaxel.Mechanistically,decreased LIN9 in resistant cell lines reduced tumor cell viability,promoted multinucleated cells formation and induced tumor cell apoptosis,potentially by directly regulating microtubule-binding protein CCSAP.In conclusion,high LIN9 expression contributed to poor clinical outcomes and paclitaxel resistance in TNBC and BETi,targeting LIN9 expression,could be a reversible drug for PTX-resistant TNBC patients. | Hongna Lai Rui Wang Shunying Li Qianfeng Shi Zijie Cai Yudong Li Yujie Liu | 2020 | Science China(Life Sciences)2020，63，3： | 2 |
| 10 | Representing surface wind stress response to mesoscale SST perturbations in western coast of South America using Tikhonov regularization method显示文摘Interaction between mesoscale perturbations of sea surface temperature(SSTmeso)and wind stress(WSmeso)has great influences on the ocean upwelling system and turbulent mixing in the atmospheric boundary layer.Using daily Quik-SCAT wind speed data and AMSR-E SST data,SSTmeso and WSmeso fields in the western coast of South America are extracted by using a locally weighted regression method(LOESS).The spatial patterns of SSTmeso and WSmeso indicate strong mesoscale SST-wind stress coupling in the region.The coupling coefficient between SSTmeso and WSmeso is about 0.0095 N/(m^2·℃)in winter and 0.0082 N/(m^2·℃)in summer.Based on mesoscale coupling relationships,the mesoscale perturbations of wind stress divergence(Div(WSmeso))and curl(Curl(WSmeso))can be obtained from the SST gradient perturbations,which can be further used to derive wind stress vector perturbations using the Tikhonov regularization method.The computational examples are presented in the western coast of South America and the patterns of the reconstructed WS meso are highly consistent with SSTmeso,but the amplitude can be underestimated significantly.By matching the spatially averaged maximum standard deviations of reconstructed WSmeso magnitude and observations,a reasonable magnitude of WSmeso can be obtained when a rescaling factor of 2.2 is used.As current ocean models forced by prescribed wind cannot adequately capture the mesoscale wind stress response,the empirical wind stress perturbation model developed in this study can be used to take into account the feedback effects of the mesoscale wind stress-SST coupling in ocean modeling.Further applications are discussed for taking into account the feedback effects of the mesoscale coupling in largescale climate models and the uncoupled ocean models. | CUI Chaoran ZHANG Rong-Hua WANG Hongna WEI Yanzhou | 2020 | Journal of Oceanology and Limnology2020，38，3： | 2 |
| 11 | Optimal design of heat exchanger header for coal gasification in supercritical water through CFD simulations显示文摘Heat exchangers play an important role in supercritical water coal gasification systems for heating feed and cooling products.However,serious deposition and plugging problems always exist in heat exchangers.CFD modeling was used to simulate the transport characteristics of solid particles in supercritical water through the shell and tube of heat exchangers to alleviate the problems.In this paper,we discuss seven types of exchangers(A,B,C,D,E,F and G),which vary in inlet nozzle configuration,header height,inlet pipe diameter and tube pass distribution.In the modeling,the possibility of deposition in the header was evaluated by accumulated mass of particles;we used the velocity contour of supercritical water(SCW)to evaluate the uniformity of the velocity distribution among the tube passes.Simulation results indicated that the optimum heat exchanger had structure F,which had a rectangular configuration of tube pass distractions,a bottom inlet,a 200-mm header height and a 10-mm inlet pipe diameter. | Lei Huang Lin Qi Hongna Wang Jinli Zhang Xiaoqiang Jia | 2017 | Chinese Journal of Chemical Engineering2017，25，8： | 1 |
| 12 | Mutations in the miRNA165/166 binding site of the HB2 gene result in pleiotropic effects on morphological traits in wheat显示文摘Leaf,spike,stem,and root morphologies are key factors that determine crop growth,development,and productivity.Multiple genes that control these morphological traits have been identified in Arabidopsis,rice,maize,and other plant species.However,little is known about the genomic regions and genes associated with morphological traits in wheat.Here,we identified the ethyl methanesulfonate-derived mutant wheat line M133 that displays multiple morphological changes that include upward-curled leaves,paired spikelets,dwarfism,and delayed heading.Using bulked segregant RNA sequencing(BSR-seq)and a high-resolution genetic map,we identified TraesCS1D02G155200(HBD2)as a potential candidate gene.HB-D2 encodes a class III homeodomain-leucine zipper(HD-ZIP III)transcription factor,and the mutation was located in the miRNA165/166 complementary site,resulting in a resistant allele designated rHb-D2.The relative expression of rHb2 in the mutant plants was significantly higher(P<0.01)than in plants homozygous for the WT allele.Independent resistant mutations that disrupt the miRNA165/166 complementary sites in the A-(rHb-A2)and B-genome(rHb-B2)homoeologs showed similar phenotypic alterations,but the relative intensity of the effects was different.Transgenic plants expressing rHb-D2 gene driven by the maize UBIQUITIN(UBI)promoter showed similar phenotypes to the rHb-D2 mutant.These results confirmed that HB-D2 is the causal gene responsible for the mutant phenotypes.Finally,a survey of 1397 wheat accessions showed that the complementary sites for miRNA165/166 in all three HB2 homoeologs are highly conserved.Our results suggest that HB2 plays an important role in regulating growth and development in wheat. | Dengji Jiang Lei Hua Chaozhong Zhang Hongna Li Zheng Wang Jian Li Guiping Wang Rui Song Tao Shen Hongyu Li Shengsheng Bai Yanna Liu Jian Wang Hao Li Jorge Dubcovsky Shisheng Chen | 2023 | The Crop Journal2023，11，1： | 1 |
| 13 | The PMIP3 Simulated Climate Changes over Arid Central Asia during the Mid-Holocene and Last Glacial Maximum显示文摘In this study, the climate changes over Arid Central Asia(ACA) during the mid-Holocene(approximately 6,000 calendar years ago, MH) and the Last Glacial Maximum(approximately 21,000 calendar years ago, LGM) were investigated using multimodel simulations derived from the Paleoclimate Modelling Intercomparison Project Phase 3(PMIP3). During the MH, the multimodel median(MMM) shows that in the core region of ACA, the regionally averaged annual surface air temperature(SAT) decreases by 0.13°C and annual precipitation decreases by 3.45%, compared with the preindustrial(PI) climate. The MMM of the SAT increases by 1.67/0.13°C in summer/autumn, whereas it decreases by 1.23/1.11°C in spring/winter. The amplitude of the seasonal cycles of the SAT increases over ACA due to different MH orbital parameters. For precipitation, the regionally averaged MMM decreases by 5.77%/5.69%/0.39%/5.24% in spring/summer/autumn/winter, respectively. Based on the analysis of the aridity index(AI), compared with the PI, a drier climate appears in southern Central Asia and western Xinjiang due to decreasing precipitation. During the LGM, the MMM shows that the regionally averaged SAT decreases by 5.04/4.36/4.70/5.12/5.88°C and precipitation decreases by 27.78%/28.16%/31.56%/27.74%/23.29% annually and in the spring, summer, autumn, and winter, respectively. Robust drying occurs throughout almost the whole core area. Decreasing precipitation plays a dominant role in shaping the drier conditions, whereas strong cooling plays a secondary but opposite role. In response to the LGM external forcings, over Central Asia and Xinjiang, the seasonal cycle of precipitation has a smaller amplitude compared with that under the PI climate. In the model-data comparison, the simulated MH moisture changes over ACA are to some extent consistent with the reconstructions, further confirming that drier conditions occurred during that period than during the PI. | XU Hongna WANG Tao WANG Huijun MIAO Jiapeng CHEN Jianhui CHEN Shengqian | 2020 | Acta Geologica Sinica(English Edition)2020，94，3： | 1 |
| 14 | LC‐MS/MS method for the determination of agomelatine in human plasma and its application to a pharmacokinetic study显示文摘 | Xiaolin Wang Dan Zhang Man Liu Hongna Zhao Aihua Du Lingjie Meng Huichen Liu | 2014 | Biomed Chromatogr2014，，2： | 1 |
| 15 | Computational fluid dynamics simulation of a novel bioreactor forsophorolipid production显示文摘This paper describes three-dimensional computational fluid dynamics(CFD) simulations of gas–liquid flow in a novel laboratory-scale bioreactor contained dual ventilation-pipe and double sieve-plate bioreactor(DVDSB)used for sophorolipid(SL) production. To evaluate the role of hydrodynamics in reactor design, the comparisons between conventional fed-batch fermenter and DVDSB on the hydrodynamic behavior are predicted by the CFD methods. Important hydrodynamic parameters of the gas–liquid two-phase system such as the liquid phase velocity field, turbulent kinetic energy and volume-averaged overall and time-averaged local gas holdups were simulated and analyzed in detail. The numerical results were also validated by experimental measurements of overall gas holdups. The yield of sophorolipids was significantly improved to 484 g·L^(-1)with a 320 h fermentation period in the new reactor. | Xiaoqiang Jia Lin Qi Yaguang Zhang Xue Yang Hongna Wang Fanglong Zhao Wenyu Lu | 2017 | Chinese Journal of Chemical Engineering2017，25，6： | 1 |
| 16 | Ru Nanoparticles Entrapped in Mesopolymers for Efficient Liquid-phase Hydrogenation of Unsaturated Compounds显示文摘 | Liying Song Xiaohong Li Hongna Wang | 2009 | Catalysis Letters2009，133，： | 1 |
| 17 | Insight to the enhanced microwave absorption of porous N-doped carbon driven by ZIF-8:Competition between graphitization and porosity显示文摘Porous carbon-based materials are promised to be lightweight dielectric microwave absorbents.Deeply understanding the influence of graphitization grade and porous structure on the dielectric parameters is urgently required.Herein,utilizing the low boiling point of Zn,porous N-doped carbon was fabricated by carbonization of ZIF-8(Zn)at different temperature,and the microwave absorption performance was investigated.The porous N-doped carbon inherits the high porosity of ZIF-8 precursor.By increasing the carbonization temperature,the contents of Zn and N elements are decreased;the graphitization degree is improved;however,the specific surface area and porosity are increased first and then decreased.When the carbonization temperature is 1000°C,the porous N-doped carbon behaves enhanced microwave absorption.With an ultrathin thickness of 1.29 mm,the ideal RL reaches-50.57 dB at 16.95 GHz and the effective absorption bandwidth is 4.17 GHz.The mechanism of boosted microwave absorption is ascribed to the competition of graphitization and porosity as well as N dopants,resulting in high dielectric loss capacity and good impedance matching.The porous structure can prolong the pathways and raise the contact opportunity between microwaves and porous carbon,causing multiple scattering,interface polarization,and improved impedance matching.Besides,the N dopants can induce electron polarization and defect polarization.These results give a new insight to construct lightweight carbon-based microwave absorbents by regulating the graphitization and porosity. | You Zhou Hongpeng Wang Dan Wang Xianfeng Yang Hongna Xing Juan Feng Yan Zong Xiuhong Zhu Xinghua Li Xinliang Zheng | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，3： | 1 |
| 18 | Ru Nanoparticles Entrapped in Mesopolymers for Efficient Liquid-phase Hydrogenation of Unsaturated Compounds显示文摘 | Song Liying Li Xiaohong Wang Hongna | 2009 | Catalysis Letters2009，133，： | 1 |
| 19 | High capacity adsorption of antimony in biomass-based composite and its consequential utilization as battery anode显示文摘Antimony is more than an emerging pollutant in water but a scare resource.In this study,we report an adsorbent with the record capacity so far from the balanced view of Sb(Ⅲ) and Sb(Ⅴ).The composite adsorbent was fabricated by encapsulating hollow Fe_(3)O_(4)nanosphere with the EDTA grafted chitosan,and it has superhigh adsorption capacity of for 657.1 mg/g for Sb(Ⅲ) and 467.3 mg/g for Sb(Ⅴ),respectively.The mechanism study reveals that the adsorption of Sb initializes from the Fe_(3)O_(4),propagates along the chitosan with hydrogen bond,and terminates at the inner sphere complex with the EDTA moiety in the adsorbent.In view of the ultra-high adsorption capacity of the adsorbent,the recovered adsorbent that contains abundant (>36.4%) highly dispersed antimony nanoparticles (600-FCSE-Sb) is applied to Li-ion battery anode after reduction.This article provides a new idea for connecting water treatment and electric energy storage. | Yao Wang Xinyue Zhang Na Ju Hongna Jia Zejun Sun Jiaxing Liang Rongxiu Guo Dun Niu Hong-bin Sun | 2023 | Journal of Environmental Sciences2023，，4： | 1 |
| 20 | The composition, size and hydration of yak casein micelles 显示文摘 | Wang Pengjie Liu Hongna Wen Pengcheng | 2013 | International Dairy Journal2013，31，2： | 1 |
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