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| 1 | Electroporation: A New Approach Enhancing Antitumor Effects of Cytoxan显示文摘Electrochemotherapy (ECT) is a novel cancer treatment in which electric pulses (EPs) inducing cell membrane pored (electroporation) are used as a means of delivering antitumor drugs to the cytoplasm of cancer cells. In vitro, with scan electromicroscope (SEM) and Trypan blue staining examination, the best parameter of EPs of electroporation is studied by the S-180 cells exposed to EP with various voltages, pulses , capacitance. The best parameter of EP of electroporation is 600V/cm, 6 pulses, 10 μF. In the in vivo study, ECT is studied with the Cytoxan (CTX) injected directly into the tumor followed immediately by a local EP at the tumor site. Four parameters, which include the tumor inhibitory ratio, the curing ratio and the vas capillare of tumor, the tumor’s histopathological characteristics are determined and compared among the ECT group, the control group, the EP-only group and the drug-only group. The results indicate that the antitumor effect of CTX is significantly enhanced by electroporation. | 杨孔 YUE Bisong Wang Zishu ZOU Fangdong Zhao Ermi WANG Baoyi Zhang Hong | 2003 | High Technology Letters2003，9，3： | 5 |
| 2 | Impact of the Qinghai-Tibet Railway on Population Genetic Structure of the Toad-Headed Lizard,Phrynocephalus vlangalii显示文摘Using data from nine microsatellite DNA loci and a population genetic approach,we evaluate the barrier effect of the Qinghai-Tibet Railway on toad-headed lizard,Phrynocephalus vlangalii. The study area is along a 20 km stretch of the railway on northern Qinghai-Tibet Plateau,and this section of the railway was constructed between 1958–1979. Both assignment tests and analysis of molecular variance(AMOVA) were used for data analysis. We found significant genetic differentiation between the populations from the study area and those from a further southeastern area,which are separated by a 20 km gap. This suggests the existence of population substructure at a fine-scale. However,we did not detect any difference between samples from the western and eastern sides of the railway within the study area,and concluded that the railway may not impose a significant barrier effect on these lizard populations at the present time. Available suitable habitat alongside the railway and bridge underpasses may have facilitated the gene exchange between the sides. The relatively short time since the completion of the railway may not allow the differentiation to accumulate to a detectable level. Since the Qinghai-Tibet Plateau maintains a unique and fragile ecosystem,long-term monitoring of such man-made landscape features is imperative for protecting this ecosystem. | Dun HU Jinzhong FU Fangdong ZOU Yin QI | 2012 | Asian Herpetological Research2012，3，4： | 1 |
| 3 | Phylogenetic and molecular dating analysis of Taiwan Blue Pheasant ( Lophura swinhoii )显示文摘 | Lichun Jiang Gaochao Wang Rui Peng Quekun Peng Fangdong Zou | 2014 | Gene2014，，： | 1 |
| 4 | Molecular cloning and sequence analysis of interferon-gamma and interleukin-6 from Tibetan macaque (Maeaca thibetana)显示文摘 | Wei Kun Liang Xu Zou Fangdong | 2006 | Veterinary Immunology and Immunopathology2006，114，34： | 1 |
| 5 | Isolation and characterization of microsatellite loci from forest musk deer(Moschus berezovskii)显示文摘 | Fangdong Zou Bisong Yue Liu Xu | 2005 | Zoological Science2005，22，： | 1 |
| 6 | Nanog reporter system in mouse embryonic stem cells based on highly efficient BAC homologous recombination显示文摘Nanog is a novel transcription factor specifically expressed in mouse embryonic stem cells (mES cells). It has been reported that Nanog plays an essential role in maintaining multi-potency of ES cells. The expression of Nanog is very sensitive to ES cells differentiation, making Nanog one of the best markers to indicate the status of ES cells. In this study, we developed an efficient method to construct Nanog promoter driven EGFP reporter system based on the BAC homologous recombination. We further generated a Nanog-EGFP reporter mES cell line. This reporter mES cell line exhibited features similar to those of normal mES cells, and the EGFP reporter efficiently reflected the expression of Nanog, indi- cating the differentiation status of mES cells. We achieved a reliable experimental reporter system to research self-renewal and differentiation of mES cells. The system could facilitate research on culture system of mES cells and researches on the expression and regulation of Nanog and other related fac- tors in mES cells. | FAN Yan TONG Zan YOU JieFang DU LiYing ZOU FangDong DING MingXiao DENG HongKui | 2007 | Chinese Science Bulletin2007，52，20： | 0 |
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