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| 1 | Anti-apoptotic effects of aspirin following cerebral ischemia-reperfusion injury in rats显示文摘BACKGROUND:The pharmacological effects of aspirin on apoptosis are complex.The underlying mechanisms have not been properly defined.OBJECTIVE:To observe the effect of different doses of aspirin on brain cell apoptosis following focal cerebral ischemia-reperfusion injury(CIRI) in rats.DESING,TIME AND SETTING:A randomized,controlled,animal experiment,performed at the School of Medicine and Pharmaceutics,Jiangnan University between June and October 2006.MATERIALS:Twenty-six male,adult,Sprague Dawley rats(grade II),weighing 240-290 g,were obtained from Shanghai Experimental Animal Center,Chinese Academy of Sciences.Aspirin was provided by Sigma(USA).METHODS:The rats were randomly divided into four groups:sham-operation(SO),CIRI + vehicle,CIRI + aspirin(6 mg/kg),and CIRI + aspirin(60 mg/kg).Rats in the lesion groups were intragastrically administrated saline,aspirin(6 mg/kg),or aspirin(60 mg/kg),respectively.MAIN OUTCOME MEASURES:The number of pyramidal neurons with normal appearance in the cerebral cortex at 2-4 mm from the midline;apoptotic cell death as measured by TUNEL;Bcl-2 and Bax protein localization was determined by immunohistochemistry;malondialdehyde(MDA) and super oxidation(SOD) content were determined by biochemistry method;adenosine triphosphate(ATP) content measured by capillary electrophoresis.RESULTS:Following CIRI,the following parameters were altered compared with sham-operated animals:the number of neurons with normal appearance was significantly reduced in the cerebral cortex;the number of apoptotic cells increased;Bax protein expression was enhanced;and the ratio between Bcl-2 and Bax decreased.In addition,MDA content increased significantly,whereas ATP content decreased(P < 0.01).Aspirin ameliorated the loss of healthy pyramidal neurons.Both 6 and 60 mg/kg aspirin increased the ratio between Bcl-2 and Bax,with no significant difference between the treatment groups.In addition,60 mg/kg aspirin decreased MDA content and increased ATP levels.However,6 mg/kg aspirin did not have the same effect.CONCLUSION:Aspirin reduced the number of apoptotic cells following CIRI.These results suggest that the neuroprotective mechanism of aspirin could be related to elevated Bcl-2 protein levels or decreased Bax protein expression.The increase in the ratio of Bcl-2 to Bax appears to be a common anti-apoptotic mechanism of aspirin. | Liying Qiu Bin Du Ying Li Hongbin Fan Zhiyong Yang | 2008 | Neural Regeneration Research2008，3，9： | 4 |
| 2 | Changes in neuronal apoptosis and apoptosis modulatory factors in cerebral ischemia/reperfusion显示文摘BACKGROUND: The high concentration of glutamate release is the main cause for neuronal cell death. The relationship between glutamate level and apoptosis during ischemia/reperfusion injury is still unclear. OBJECTIVE: To observe the neuronal apoptosis at 24 and 72 hours following cerebral ischemia/reperfusion in rats,and analyze the possible influencing factors. DESIGN: A randomized controlled animal experiment. SETTING: School of Medicine,Southern Yangtze University. MATERIALS: Totally 30 male adult Sprague Dawley (SD) rats of clean grade,weighing 240-290 g,were obtained from Shanghai Experimental Animal Center,Chinese Academy of Sciences. The rats were randomly divided into sham-operated group (n=10) and model group (n=20). Each group was observed at 24 and 72 hours after ischemia/reperfusion,5 rats at each time point in the sham-operated group,whereas 12 at 24 hours and 8 at 72 hours in the model group. Kits for determining apoptosis and Bcl-2 were bought from Wuhan Boster Biological Technology,Co.,Ltd.; Kit for calcineurin from Nanjing Jiancheng Bioengineering Institute. METHODS: The experiment was carried out in the Functional Scientific Research Room of Southern Yangtze University from June to October in 2006. ① Right middle cerebral artery was occluded by inserting a thread through internal carotid artery (ICA). The surgical process for the sham-operated rats was the same as that in the model group except a nylon suture inserted the ICA. According to Longa five-degree standard,the neurological deficit evaluation of rats was evaluated after surgery,and grades 1-3 were taken as successful model establishment. The blood was recirculated by withdrawing the nylon filament under anesthesia at 2 hours after ischemia in successful rat models. ②After reperfusion,the brain tissue was quickly removed at 24 or 72 hours and the slices were obtained from optic chiasma to funnel manubrium. The changes of the number of apoptotic cells were observed using the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick-end labeling method. The expressions of Bcl-2 protein were determined with immunohistochemical staining. The activity of calcineurin was determined by the inorganic phosphorus method. The content of excitatory amino acid was detected by high performance liquid chromatography. MAIN OUTCOME MEASURES: ① Glutanate content in brain tissue; ② Conditions of apoptosis; ③ Calcineurin activity in brain tissue; ④ Bcl-2 expression in brain tissue. RESULTS: Totally 30 SD rats were used,5 died and the other 25 were involved in the analysis of results. ① Changes of apoptosis: There were 0-3 apoptotic cells in the sham-operated group. In the model group,the numbers of apoptotic cells were obviously increased at 24 and 72 hours of reperfusion (P < 0.01),and it was markedly reduced at 72 hours as compared with 24 hours (P < 0.01). ② Changes of glutanate content: The glutamate contents at 24 and 72 hours of reperfusion in the model group were obviously higher than those in the sham-operated group (P < 0.01); In the model group,it was obviously increased at 24 hours as compared with 72 hours (P < 0.01). ③ Changes of Bcl-2 protein: In the model group,the Bcl-2 protein expression had no obvious changes at 24 hours of reperfusion,and it was obviously enhanced at 72 hours,which was obviously different from that in the sham-operated group and that at 24 hours (P < 0.01). ④ Changes of calcinerin activity: In the model group,the activity of calcineurin in brain tissue had no obvious changes at 24 hours of reperfusion; The activity of calcineurin at 72 hours was obviously higher than that in the sham-operated group and that at 24 hours (P < 0.01). CONCLUSION: The brain cyto-apoptosis action at different time points following reperfusion incompletely depends on the glutamate levels,while it depends on the interaction of some apoptosis related factors,such as amino acid,calcineurin,and Bcl-2,etc. | Liying Qiu Ying Li Hongbin Fan Bin Du Zhiyong Yang Jianqing Cheng | 2007 | Neural Regeneration Research2007，2，6： | 3 |
| 3 | Cholecystokinin octapeptide antagonizes apoptosis in human retinal pigment epithelial cells显示文摘Although cholecystokinin octapeptide-8 is important for neurological function, its neuroprotective properties remain unclear. We speculated that cholecystokinin octapeptide-8 can protect human retinal pigment epithelial cells against oxidative injury. In this study, retinal pigment epithelial cells were treated with peroxynitrite to induce oxidative stress. Peroxynitrite triggered apoptosis in these cells, and increased the expression of Fas-associated death domain, Bax, caspase-8 and Bcl-2. These changes were suppressed by treatment with cholecystokinin octapeptide-8. These results suggest that cholecystokinin octapeptide-8 can protect human retinal pigment epithelial cells against apoptosis induced by peroxynitrite. | Yuan Liu Yueling Zhang Zhaohui Gu Lina Hao Juan Du Qian Yang Suping Li Liying Wang Shilei Gong | 2014 | Neural Regeneration Research2014，9，14： | 2 |
| 4 | c-Src regulates cell cycle proteins expression through protein kinase B/glycogen synthase kinase 3 beta and extracellular signal-regulated kinases 1/2 pathways in MCF-7 cells显示文摘我们表明了禁止的那 c-Src 抑制对在人的乳癌房间的间充质的转变上皮。这里,我们用 siRNAs 和小分子禁止者 4-amino-5-(4-chlorophenyl ) 在房间周期前进上调查了 c-Src 的角色 -7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2 ) 。西方的污点分析由 PP2 在 c-Src 抑制以后表明了 cyclin D1 和 cyclin E 的下面规定和 p27 Kip1 的起来规定。房间的孵化显著地面对 PP2 堵住了细胞外的调整信号的 kinases 的 phosphorylation 1/2 (ERK1/2 ) ,蛋白质 kinase B (AKT ) ,和肝糖 synthase kinase 3 贝它(GSK3 ) 。MEK1/2/ERK1/2 和 phosphatidylinositide 3-kinase/AKT 小径的特定的药理学禁止者被用来表明在信号串联和房间周期蛋白质表示之间的关系。cyclin D1 和 cyclin E 的表示在 ERK1/2 或 AKT 活动的抑制以后被减少,而 p27 Kip1 表示被增加。另外,由 siRNAs 的 c-Src 击倒减少的房间增长和 ERK1/2, AKT,和 GSK3 的 phosphorylation。在由 siRNAs 的 c-Src 弄空以后,我们观察了 p27 Kip1 的 cyclin D1 和 cyclin E,和起来规定的重要下面规定。这些结果由 PP2 建议那 c-Src 抑制或 siRNAs 可以通过 AKT/GSK3 和 ERK1/2 小径调整房间周期的前进。 | Xiang Liu Liying Du Renqing Feng | 2013 | Acta Biochimica et Biophysica Sinica2013，45，7： | 2 |
| 5 | Angle-of-arrival fluctuations for wave propagation through non-kolmogorov turbulence 显示文摘 | Du Wenhe Yu Siyuan Tan Liying | 2009 | Opt Commun2009，282，5： | 1 |
| 6 | T-614, a novel immunomodulator, attenuates joint inflammation and articular damage in collagen- induced arthritis显示文摘 | Du F Lii LJ Fu Q | 2008 | Arthritis Res Ther2008，10，6： | 1 |
| 7 | T-614 alters the production of matrix metalloproteinases (MMP-1 and MMP-3) and inhibits the migratory expansion of rheumatoid synovial fibroblasts, in vitro 显示文摘 | Du F Lii LJ Teng JL | 2012 | Int Immunopharmacol2012，13，1： | 1 |
| 8 | Function projective synchronization in complex dynamical networks with time delay via hybrid feedback control显示文摘 | Du H Y Shi P Lii N | 2013 | Nonlinear Analysis: Real World Applications2013，14，2： | 1 |
| 9 | Smac,a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition显示文摘 | Du C Fang M LiI Y | 2000 | Cell2000，102，1： | 1 |
| 10 | A crosstalk between auxin and brassinosteroid regulates leaf shape by modulating growth anisotropy显示文摘Leaf shape is highly variable within and among plant species,ranging from slender to oval shaped.This is largely determined by the proximodistal axis of growth.However,little is known about how proximal–distal growth is controlled to determine leaf shape.Here,we show that Arabidopsis leaf and sepal proximodistal growth is tuned by two phytohormones.Two class A AUXIN RESPONSE FACTORs(ARFs),ARF6 and ARF8,activate the transcription of DWARF4,which encodes a key brassinosteroid(BR)biosynthetic enzyme.At the cellular level,the phytohormones promote more directional cell expansion along the proximodistal axis,as well as final cell sizes.BRs promote the demethyl-esterification of cell wall pectins,leading to isotropic in-plane cell wall loosening.Notably,numerical simulation showed that isotropic cell wall loosening could lead to directional cell and organ growth along the proximodistal axis.Taken together,we show that auxin acts through biosynthesis of BRs to determine cell wall mechanics and directional cell growth to generate leaves of variable roundness. | Yuanyuan Xiong Binbin Wu Fei Du Xiaolu Guo Caihuan Tian Jinrong Hu Shouqin Lii Mian Long Lei Zhang Ying Wang Yuling Jiao | 2021 | Molecular Plant2021，14，6： | 1 |
| 11 | MiRNA- miRNA synergistic network : construction via co-regulating functional modules and disease miRNA topological features显示文摘 | XuJ LiCX LiYS LvJY MaY Shao T T XuLD WangY Y Du L ZhangY P Jiang W Li C Q Xiao Y Li X | 2011 | Nucleic Acids Research2011，39，3： | 1 |
| 12 | Drivers of the development of global climate-change-mitigation technology:a patent-based decomposition analysis显示文摘The development of the climate-change-mitigation technology has received widespread attention from both academic and policy studies.Nevertheless,very few studies have explained how and why economies contribute differently to global development.This paper decomposed the development of the global climate-change-mitigation technology,proxied by patent-based indicators,from 1996 to 2015 into several predefined factors.The results show that the worldwide surge of climate-change-mitigation-technology patents from 1996 to 2011 is driven by increased concentration on green invention,improved research intensity,and enlarged economic scale,while the falling of patent counts from 2011 to 2015 is predominantly due to less concentration on green invention.Among different climate-change-mitigation technologies,the type-specific development is attributed to different dominant factors,and the resulting priority change can reflect the shift of both global research and development(R&D)resource and market demand.Regarding regional contributions,the resulting economy-specific contributions to each driving factor can be used to design the policies to promote the development of the global climate-change-mitigation technology. | Liying SONG Jun JING Kerui DU Zheming YAN | 2021 | Frontiers in Energy2021，15，2： | 0 |
| 13 | Highly stable aqueous phase black phosphorus quantum dots with enhanced fluorescence property显示文摘Black phosphorus quantum dots(BPQDs)are a kind of outstanding optical material due to the tunable band structure.However,their fluorescence property and stability are obviously weakened in the aqueous phase,which extremely restricts the development of BPQDs.Here,we propose a new method to prepare stable BPQDs in an aqueous solution directly with high absolute fluorescence quantum yield.Aqueous phase BPQDs are synthesized by liquid exfoliation and hydrothermal with NH_(2)-polyethylene glycol(PEG)-NH_(2) as the modification agent to protect the BPQDs,which have high stability more than six months.In addition,NH_(2)-PEG-NH_(2) is also a surface passivation agent to enhance the emission of BPQDs by forming P-N bonds,which is confirmed by an absolute fluorescent quantum yield of 11.5%.Moreover,BPQDs show excellent resistance to a strong acid environment and high ionic strengths except for Fe^(3+).Therefore,the BPQDs are a kind of highly selective and linear response fluorescence probe for Fe^(3+).Considering the good biocompatibility of BPQDs,they are employed as cell fluorescent label probes and show excellent fluorescence imaging contrast.Based on the unique structural stability and fluorescence performance in the aqueous phase,BPQDs are potential candidates for Fe^(3+)detection and optical bio-imaging. | Yingli Ma Xiaoguang Xu Xiaoshuang Zhang Mayifei Rong Liying Lu Yan Li Wenhao Dai Hongwu Du Yong Jiang | 2022 | Journal of Materials Science & Technology2022，，21： | 0 |
| 14 | Short-Term Prediction of Photovoltaic Power Based on Fusion Device Feature-Transfer显示文摘To attain the goal of carbon peaking and carbon neutralization,the inevitable choice is the open sharing of power data and connection to the grid of high-permeability renewable energy.However,this approach is hindered by the lack of training data for predicting new grid-connected PV power stations.To overcome this problem,this work uses open and shared power data as input for a short-term PV-power-prediction model based on feature transfer learning to facilitate the generalization of the PV-power-prediction model to multiple PV-power stations.The proposed model integrates a structure model,heat-dissipation conditions,and the loss coefficients of PV modules.Clear-Sky entropy,characterizes seasonal and weather data features,describes the main meteorological characteristics at the PV power station.Taking gate recurrent unit neural networks as the framework,the open and shared PV-power data as the source-domain training label,and a small quantity of power data from a new grid-connected PV power station as the target-domain training label,the neural network hidden layer is shared between the target domain and the source domain.The fully connected layer is established in the target domain,and the regularization constraint is introduced to fine-tune and suppress the overfitting in feature transfer.The prediction of PV power is completed by using the actual power data of PV power stations.The average measures of the normalized root mean square error(NRMSE),the normalized mean absolute percentage error(NMAPE),and the normalized maximum absolute percentage error(NLAE)for the model decrease by 15%,12%,and 35%,respectively,which reflects a much greater adaptability than is possible with other methods.These results show that the proposed method is highly generalizable to different types of PV devices and operating environments that offer insufficient training data. | Zhongyao Du Xiaoying Chen Hao Wang Xuheng Wang Yu Deng Liying Sun | 2022 | Energy Engineering2022，119，4： | 0 |
| 15 | A smart nanoprobe based on luminescent terbium metal-organic framework coated gold nanorods for monitoring and photo-stimulated combined thermal-chemotherapy显示文摘Real-time monitoring and accurate treatment are of great significance for reducing the side effects during the cancer therapy.In this work,we rationally prepared a smart nanoprobe based on core-shell terbium metal-organic gramework(MOF)with highly effective photothermal conversion capacity and drug release efficiency under near-infrared light irradiation for real-time fluorescence imaging-guided multimodal therapy.The nanoprobe can achieve prominent photothermal therapy and controllable drug release,act as a fluorescence thermometer,and also real-time feedback of temperature is expected to be achieved.At the same time,the two-photon ligand design significantly improves the tissue penetration ability of the nanoprobe.Our work is expected to provide a reference for precise treatment and real-time monitoring of tumors and at the same time provide new ideas for the formulation of individualized treatment plans. | Tong Zhang Liying Qin Liangliang Liu Meina Zhang Tianyi Du Yifan Fan Huicheng Yan Pingru Su Ping Zhou Yu Tang | 2022 | Journal of Rare Earths2022，40，9： | 0 |
| 16 | Application of biofl oc technology in recirculation A rtemia culture system显示文摘Biofl oc technology(BFT)improves water quality,and productivity of the farmed species through converting ammonium nitrogen to microbial protein,stabilizing microbial community,and reducing the production cost.In this study,a small-scale biofl oc development unit was designed in combination of recirculation system(RAS)for Artemia culture.Artemia growth,water quality,and microbial composition of biofl ocs in RAS were studied in comparison with in-situ batch culture(Glu).Glucose was added in RAS and Glu at C/N ratio of 10.The cultures without glucose addition,but with 50%daily water renewal(WRe)and without water renewal(NWRe)were considered as the controls.Arte mia were cultured at 25℃ and salinity 30 for 24 days and fed formulated feed.The results showed that compared to the controls,Glu signifi cantly improved the Artemia biomass,increased the biofl oc volume,and reduced the content of total ammonia nitrogen(TAN),nitrite nitrogen(NO_(2)-N)and nitrate nitrogen(NO_(3)-N)in water column(P<0.05).In addition,RAS had similar results with Glu.High throughput sequencing analysis on biofl oc microbial composition demonstrated that glucose supplement shaped the microbial community structure,and increased proportion of potential probiotic bacteria and suppressed pathogenic bacteria growth.Furthermore,we analyzed the relationship between the microbial composition of biofl oc and environmental factors.Canonical correspondence analysis(CCA)indicated that inorganic nitrogen in culture water had great impact on biofl oc microbial composition in NWRe and WRe,whilst the dissolved organic carbon(DOC)modifi ed the microbial community in Glu and RAS.This study shows the advantages of BFT in Artemia culture and provides practical information for applying BFT-RAS in indoor Artemia culture. | Xuejiao LIANG Chi ZHANG Dongdong DU Meirong GAO Liying SUI | 2022 | Journal of Oceanology and Limnology2022，40，4： | 0 |
| 17 | Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids显示文摘Oxidative stress induced by factors such as ammonia nitrogen has become a major issue in shrimp farming.The effects of carotenoids on the growth and antioxidant capability of Litopenaeus vannamei juveniles were investigated in this study using dietary archaeal carotenoids supplementation.For four weeks,shrimp were given diets containing 0 mg/kg(Ctrl)and 55.98 mg/kg(Car)archaeal carotenoids.Dietary archaeal carotenoids significantly enhanced the astaxanthin content in shrimp muscles and carapaces,as well as the superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activity(P<0.05).The malonaldehyde(MDA)content in Car group significantly decreased(P<0.05).The transcriptome analysis was conducted to determine the molecular processes in response to archaeal carotenoids supplementation.A total of 1536 differentially expressed genes(DEGs)were detected,including 538 upregulated DEGs and 998 downregulated DEGs.GO functional enrichment analysis between Ctrl and Car indicated that 26 GO terms including extracellular region,metabolic process,and proteolysis were enriched.The KEGG pathway enrichment analysis revealed that the amino sugar and nucleotide sugar metabolism,cysteine and methionine metabolism,glycine serine and threonine metabolism,and amino acid biosynthesis were enriched.Archaeal carotenoids influenced the expression of several important genes involved in reactive oxygen species(ROS)generation,Nrf2 signaling,and antioxidant enzymes.Seven DEGs were chosen to confirm the RNA-Seq data using qRT-PCR.The genes and pathways discovered in this work assist to elucidate the molecular processes through which archaeal carotenoid enhances L.vannamei antioxidative system. | Wei XIE Guoru DU Honggang DENG Yingchao MA Meirong GAO Hu DUAN Sung YIK YEONG Liying SUI | 2023 | Journal of Oceanology and Limnology2023，41，5： | 0 |
| 18 | Large-scale preparation of high-performance boron nitride/aramid nanofiber dielectric composites显示文摘Dielectric polymers featuring high thermal conductivity,excellent mechanical,and stable dielectric properties over a broad temperature range have attracted extensive scientific attention.In this work,a large-scale,layered film was fabricated using blade-coating approach,which integrated aramid nanofibers(ANFs)and boron nitride nanosheets(BNNSs)through a typical solgel transformation procedure.The as-prepared film with 20 wt.%BNNS displays high thermal conductivity(14.03 W·m^(−1)·K^(−1)),103-fold higher than pure ANF film,attributing to massive continuous thermal conduction pathway between BNNSs so as to facilitate fast phonon transmission.The film boasts excellent mechanical properties(stress 97.14±5.17 MPa,strain 19.36±0.35%),high degradation temperature(~542℃),a moderate dielectric constant(~6.9 at 104 Hz),together with low dielectric loss(~0.026 at 104 Hz).Meanwhile,the film reveals high breakdown voltage(310 MV·m^(−1))and volume resistivity(1013Ω·cm).Notably,these dielectric properties remain largely unchanged over a wide temperature range(25 to 200℃). | Liying Su Xiaoyan Ma Jiale Zhou Xiangchen Liu Fanglin Du Chao Teng | 2022 | Nano Research2022，15，9： | 0 |
| 19 | Nanog reporter system in mouse embryonic stem cells based on highly efficient BAC homologous recombination显示文摘Nanog is a novel transcription factor specifically expressed in mouse embryonic stem cells (mES cells). It has been reported that Nanog plays an essential role in maintaining multi-potency of ES cells. The expression of Nanog is very sensitive to ES cells differentiation, making Nanog one of the best markers to indicate the status of ES cells. In this study, we developed an efficient method to construct Nanog promoter driven EGFP reporter system based on the BAC homologous recombination. We further generated a Nanog-EGFP reporter mES cell line. This reporter mES cell line exhibited features similar to those of normal mES cells, and the EGFP reporter efficiently reflected the expression of Nanog, indi- cating the differentiation status of mES cells. We achieved a reliable experimental reporter system to research self-renewal and differentiation of mES cells. The system could facilitate research on culture system of mES cells and researches on the expression and regulation of Nanog and other related fac- tors in mES cells. | FAN Yan TONG Zan YOU JieFang DU LiYing ZOU FangDong DING MingXiao DENG HongKui | 2007 | Chinese Science Bulletin2007，52，20： | 0 |
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