|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Pancreas-specific Pten deficiency causes partial resistance to diabetes and elevated hepatic AKT signaling显示文摘PTEN, phosphatidylinositol-3-kinase/AKT 小径的一个否定管理者,是胰岛素发信号的一个重要调节的人。决定胰腺的 Pten 的新陈代谢的函数,我们产生了胰特定的 Pten 大美人(PPKO ) 鼠标。PPKO 老鼠扩大了胰并且提高了 acinar 房间的增长。他们也展出了低血糖症, hypoinsulinemia,和改变的氨基的新陈代谢。尤其是, PPKO 老鼠证明 streptozotocin (STZ ) 的推迟的发作导致了糖尿病,到 high-fat-diet (HFD ) 的偏导性的抵抗导致了糖尿病。在 PPKO 老鼠为抵抗调查机制到导致 HFD 的多糖症,我们在主要胰岛素应答的纸巾评估了 AKT phosphorylation:肝,肌肉,和脂肪。我们发现在胰的 Pten 损失引起在肝发信号的 AKT 的举起。AKT 和它的下游的底层 GSK3 尾的 phosphorylation 在 PPKO 老鼠的肝被增加,当没有 Pten 等位基因的可检测的切除, PTEN 水平在 PPKO 老鼠的肝被减少时。戏剧性地揭示的 Proteomics 分析减少了在 PPKO 老鼠的肝 78-kDa 铺平调整葡萄糖的蛋白质(GRP78 ) ,它可以也贡献用 HFD 喂的 PPKO 老鼠的更低的血葡萄糖水平。一起,我们的调查结果在新陈代谢的规定在肝揭示新奇回答到胰腺的缺点,把一种新尺寸加到理解糖尿病抵抗。 | Zan Tong Yan Fan Weiqi Zhang Jun Xu Jing Cheng Mingxiao Ding Hongkui Deng | 2009 | Cell Research2009，19，6： | 5 |
| 2 | Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair显示文摘Peripheral nerve regeneration requires stepwise and well-organized establishment of microenvironment.Since local delivery of VEGF-A in peripheral nerve repair is expected to promote angiogenesis in the microenvironment and Schwann cells(SCs)play critical role in nerve repair,combination of VEGF and Schwann cells may lead to efficient peripheral nerve regeneration.VEGF-A overexpressing Schwann cells were established and loaded into the inner wall of hydroxyethyl cellulose/soy protein isolate/polyaniline sponge(HSPS)conduits.When HSPS is mechanically distorted,it still has high durability of strain strength,thus,can accommodate unexpected strain of nerve tissues in motion.A 10 mm nerve defect rat model was used to test the repair performance of the HSPS-SC(VEGF)conduits,meanwhile the HSPS,HSPS-SC,HSPS-VEGF conduits and autografts were worked as controls.The immunofluorescent co-staining of GFP/VEGF-A,Ki67 and MBP showed that the VEGF-A overexpressing Schwann cells could promote the proliferation,migration and differentiation of Schwann cells as the VEGF-A was secreted from the VEGF-A overexpressing Schwann cells.The nerve repair performance of the multifunctional and flexible conduits was examined though rat behavioristics,electrophysiology,nerve innervation to gastrocnemius muscle(GM),toluidine blue(TB)staining,transmission electron microscopy(TEM)and NF200/S100 double staining in the regenerated nerve.The results displayed that the effects on the repair of peripheral nerves in HSPS-SC(VEGF)group was the best among the conduits groups and closed to autografts.HSPS-SC(VEGF)group exhibited notably increased CD31+endothelial cells and activation of VEGFR2/ERK signaling pathway in the regenerated nerve tissues,which probably contributed to the improved nerve regeneration.Altogether,the comprehensive strategy including VEGF overexpressing Schwann cells-mediated and HSPS conduit-guided peripheral nerve repair provides a new avenue for nerve tissue engineering. | Ping Wu Zan Tong Lihua Luo Yanan Zhao Feixiang Chen Yinping Li Céline Huselstein Qifa Ye Qingsong Ye Yun Chen | 2021 | Bioactive Materials2021，6，10： | 4 |
| 3 | Mice transgenic for human angiotensin-converting enzyme 2 provide a model for SARS coronavirus infection 显示文摘 | Yang Xiu Hong Deng Wei Tong Zan | 2007 | Comp Med2007，57，5： | 1 |
| 4 | Nanog reporter system in mouse embryonic stem cells based on highly efficient BAC homologous recombination显示文摘Nanog is a novel transcription factor specifically expressed in mouse embryonic stem cells (mES cells). It has been reported that Nanog plays an essential role in maintaining multi-potency of ES cells. The expression of Nanog is very sensitive to ES cells differentiation, making Nanog one of the best markers to indicate the status of ES cells. In this study, we developed an efficient method to construct Nanog promoter driven EGFP reporter system based on the BAC homologous recombination. We further generated a Nanog-EGFP reporter mES cell line. This reporter mES cell line exhibited features similar to those of normal mES cells, and the EGFP reporter efficiently reflected the expression of Nanog, indi- cating the differentiation status of mES cells. We achieved a reliable experimental reporter system to research self-renewal and differentiation of mES cells. The system could facilitate research on culture system of mES cells and researches on the expression and regulation of Nanog and other related fac- tors in mES cells. | FAN Yan TONG Zan YOU JieFang DU LiYing ZOU FangDong DING MingXiao DENG HongKui | 2007 | Chinese Science Bulletin2007，52，20： | 0 |
| 5 | Two‑Level Biomimetic Designs Enable Intelligent Stress Dispersion for Super‑Foldable C/NiS Nanofiber Free‑Standing Electrode显示文摘Due to the lack of in-depth understanding about the folding issues of the electronic materials,it is a huge challenge to pre-pare a super-foldable and highly electrochemical faradic electrode.Here,inspired from from the fully nimble structures of cuit cocoons and cockscomb petals,with two-level biomimetic design,for the first time we prepared a super-foldable and electrochemically functional freestanding cathode,made of C-fiber@NiS-cockscomb(SFCNi).In virtue of its nimble biomi-metic structures,SFCNi can remarkably sustain over 100,000 times,repeated true-folding without composite fibers fracture,functional matters detachment,conductivity degradation,or electrochemical performance change.The main mechanism behind these behaviors was disclosed by Real-time scanning electron microscopy and mechanical simulations,on the folding process.Results unveil that the cockscomb-like NiS with atomic thickness can deform freely due to the need of bending,and the cuit-cocoon-like SFCNi can generate an“ε-shape”folding structure at the crease.Such a smart self-adaptive deforma-tion capability can effectively reduce the effect of stresses and local excessive deformations,so that the chemical bonds can preserve their interaction,and the material won’t fracture.This subtle and exceptional mechanical behavior realizes a super-foldable property.The two-level biomimetic design strategy is a novel method for fabrication of super-foldable composite electrodes and integrated multi-functional super-foldable devices. | Guangtao Zan Tong Wu Wenya Dong Junchen Zhou Teng Tu Ruoxuan Xu Yun Chen Ying Wang Qingsheng Wu | 2022 | Advanced Fiber Materials2022，4，5： | 0 |
| 6 | Using transcriptome Shannon entropy to evaluate the off-target effects and safety of insecticidal siRNAs显示文摘A recent breakthrough in agricultural biotechnology is the introduction of RNAi-mediated strategies in pest control.However, the off-target effects of RNAi pest control are still not fully understood. Here, we studied the off-target effects of two insecticidal siRNAs in both target and non-target insects. The results revealed that off-target effects of insecticidal siRNAs occur widely in both target and non-target insects. We classified the expression-changed genes according to their homology to the siRNA-targeted gene, related KEGG pathways with the siRNA-targeted gene and continuous matches with siRNAs. Surprisingly, the unintended significant changes in gene expression levels did not strictly match with the number of contiguous nucleotides in the siRNAs. As expected, the expression of small portions of the homologous and KEGG-related genes were significantly changed. We calculated the Shannon entropy of the transcriptome profile of the insects after injecting them with insecticidal siRNAs. Though hundreds of genes were affected in their expression levels post siRNAtreatment, the Shannon entropy of the transcriptome remained unchanged, suggesting that the transcriptome expression was balanced. Our results provide evidence that siRNAs cross-reacted with individual genes in non-target species, but did not have significant effects on the integrity of the transcriptome profiles in either target or non-target species on a genomic scale. The metric we proposed can be used to estimate the off-target effects of insecticidal siRNAs, which might be useful for evaluating the safety of RNAi in pest control. | MA Wei-hua WU Tong ZHANG Zan LI Hang SITU Gong-ming YIN Chuan-lin YE Xin-hai CHEN Meng-yao ZHAO Xian-xin HE Kang LI Fei | 2022 | Journal of Integrative Agriculture2022，21，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员