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| 1 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 2 | Cyclic di-GMP signaling in bacteria: recent advances and new puzzles显示文摘 | RYAN R P FOUHY Y LUCEY J F AND DOW J M | 2007 | Nat Rev Mierobiol2007，188，24： | 1 |
| 3 | Rkaing Money from Muck 显示文摘 | Fouhy K Mooer S | 1994 | Chem Eng1994，7，： | 1 |
| 4 | The HD-GYP domain,cyclic di-GMP signaling,and bacterial virulence to plants显示文摘 | Dow JM Fouhy Y Lucey JF | 2006 | Mol Plant Microbe Interact2006，19，： | 1 |
| 5 | Composition of the early intestinal microbiota: knowledge, knowledge gaps and the use of high-throughput sequencing to ad- dress these gaps显示文摘 | Fouhy F Ross RP Fitzgerald GF | 2012 | Gut Microbes2012，3，3： | 1 |
| 6 | Altered FXR signalling is associated with bile acid dysmetabolism in short bowel syndrome-associated liver disease显示文摘 | Prue M. Pereira-Fantini Susan Lapthorne Susan A. Joyce Nicole L. Dellios Guineva Wilson Fiona Fouhy Sarah L. Thomas Michelle Scurr Colin Hill Cormac G.M. Gahan Paul D. Cotter Peter J. Fuller Winita Hardikar Julie E. Bines | 2014 | Journal of Hepatology2014，，5： | 1 |
| 7 | Divergent metabolic outcomes arising from targeted manipulation of the gut microbiota in diet-induced obesity 显示文摘 | Murphy EF Cotter PD Hogan A O'Sullivan O Joyce A Fouhy F Clarke SF Marques TM O'Toole PW Stanton C Quigley EM Daly C Ross PR OtDoherty RM Shanahan F | 2013 | Gut2013，62，2： | 1 |
| 8 | CPI design engineers go with the flow 显示文摘 | Ken Fouhy | 1995 | Chem Eng1995，，： | 1 |
| 9 | Gut microbial diversity is reduced and is associated with colonic inflammation in a piglet model of short bowel syndrome 显示文摘 | Lapthorne S Pereira-Fantini PM Fouhy F | 2013 | Gut Microbes2013，4，3： | 1 |
| 10 | The HD-GYP domain, cyclic di-GMP signaling, and bacterial virulence to plants 显示文摘 | Dow J M Fouhy Y Lucey J F | 2006 | Mol Plant Microbe Interact2006，19，12： | 1 |
| 11 | Cyclic di-GMP signalling in the virulence and environmental adaptation of Xanthomonas campestris显示文摘 | Ryan R P Fouhy Y Lucey J F | | 0，，： | 1 |
| 12 | Composition of the early in- testinal microbiota:knowledge,knowledge gaps and the use of high- throughput sequencing to address these gaps显示文摘 | Fouhy F Ross RP Fitzgerald GF | 2012 | Gut Microbes2012，3，3： | 1 |
| 13 | Microbial composition of human appendices from patients following appendectomy显示文摘 | Guinane CM Tadrous A Fouhy F | | 0，，01： | 1 |
| 14 | Cell-cell signaling in Xan- thomonas campestris involves an HD-GYP domain protein that func- tions in cyclic di-GMP turnover 显示文摘 | Ryan RP Fouhy Y Lueey JF | 2006 | Proc Natl Aead Sci USA2006，103，17： | 1 |
| 15 | Cyclic di GMP signaling in bacte- ria: recent advances and new puzzles显示文摘 | Ryan RP Fouhy Y Dow JM | 2006 | J Bacteriol2006，188，： | 1 |
| 16 | Interspecies signalling via the Stenotrophomonas maltophilia diffusible signal factor influences biofilm formation and polymyxin tolerance in Pseudomonas aerugi- nosa显示文摘 | Ryan RP Fouhy Y Garcia BF | 2008 | Mol Microbiol2008，68，1： | 1 |
| 17 | Cyclic di- GMP signalling in the virulence and environmental adap- tation ofXanthomonas campestris 显示文摘 | RYAN R P FOUHY Y LUCEY J F | 2006 | Molecular Micro- biology2006，63，2： | 1 |
| 18 | Interspecies signalling diffusible signal factor influences biofilm formation and polymyxintolerance in Pseudomonas aeruginosa 显示文摘 | RYAN R P FOUHY Y GARCIA B F | 2008 | Mol Microbiol2008，68，1： | 1 |
| 19 | Composition and energy harvesting capacity of the gut microbiota:relationship to diet,obesity and time in mouse models显示文摘 | Murphy EF Cotter PD Healy S Marques TM O'Sullivan O Fouhy F Clarke SF O'Toole PW Quigley EM Stanton C Ross PR O'Doherty RM Shanahan F | | 0，，12： | 1 |
| 20 | The HD-GYP domain, cyclic di-GMP signaling, and bacterial virulence to plants 显示文摘 | Dow J M Fouhy Y Lucey J F | 2006 | Molecular Plant-Microbe Interactions2006，19，12： | 1 |
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