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| 1 | LIM domain proteins Pinch1/2 regulate chondrogenesis and bone mass in mice显示文摘The LIM domain-containing proteins Pinch1/2 regulate integrin activation and cell–extracellular matrix interaction and adhesion.Here,we report that deleting Pinch1 in limb mesenchymal stem cells(MSCs)and Pinch2 globally(double knockout;dKO)in mice causes severe chondrodysplasia,while single mutant mice do not display marked defects.Pinch deletion decreases chondrocyte proliferation,accelerates cell differentiation and disrupts column formation.Pinch loss drastically reduces Smad2/3 protein expression in proliferative zone(PZ)chondrocytes and increases Runx2 and Col10a1 expression in both PZ and hypertrophic zone(HZ)chondrocytes.Pinch loss increases sclerostin and Rankl expression in HZ chondrocytes,reduces bone formation,and increases bone resorption,leading to low bone mass.In vitro studies revealed that Pinch1 and Smad2/3 colocalize in the nuclei of chondrocytes.Through its C-terminal region,Pinch1 interacts with Smad2/3 proteins.Pinch loss increases Smad2/3 ubiquitination and degradation in primary bone marrow stromal cells(BMSCs).Pinch loss reduces TGF-β-induced Smad2/3 phosphorylation and nuclear localization in primary BMSCs.Interestingly,compared to those from single mutant mice,BMSCs from dKO mice express dramatically lower protein levels ofβ-catenin and Yap1/Taz and display reduced osteogenic but increased adipogenic differentiation capacity.Finally,ablating Pinch1 in chondrocytes and Pinch2 globally causes severe osteopenia with subtle limb shortening.Collectively,our findings demonstrate critical roles for Pinch1/2 and a functional redundancy of both factors in the control of chondrogenesis and bone mass through distinct mechanisms. | Yiming Lei Xuekun Fu Pengyu Li Sixiong Lin Qinnan Yan Yumei Lai Xin Liu Yishu Wang Xiaochun Bai Chuanju Liu Di Chen Xuenong Zou Xu Cao Huiling Cao Guozhi Xiao | 2020 | Bone Research2020，8，4： | 2 |
| 2 | Numerical simulation of effect of rotational tool with screw on material flow behavior of friction stir welding of Ti6Al4V alloy显示文摘The rotational tool is put forward, which is composed of the one-spiral-flute shoul-der and the rotational pin with screw. Using the turbulent model of the FLUENT software, material plastic flow behavior during the process of friction stir welding of Ti6Al4V alloy is researched by the numerical simulation method and then the effect of rotational tool geometry on material flow during the welding process is attained. The results show that the flow direction of the material near the rotational tool is mainly the same as the rotational direction of the tool while the material near tool flows more violently than the other regions. For the tapered rotational pin, the flow velocity of material inside the workpiece decreases with the increase of the distance away from the workpiece surface because of the change of pin diameter. For the rotational tool, the flute added to the shoulder and the screw added to the pin can greatly increase the flow velocity of material during the welding process while the peak value of the flow velocity of material appears on the flute or the screw. Moreover, the rotational tool with the one-spiral-flute shoulder is better than the tool with the concentric-circles-flute shoulder. Decreasing the width of pin screw and increasing the diameter of pin tip are both good for the increase of flow velocity. | Shude JI Aili ZOU Yumei YUE Guohong L UAN Yanye JIN Fu LI | 2012 | Acta Metallurgica Sinica(English Letters)2012，25，5： | 2 |
| 3 | Experimental investigation of dynamic normal characteristics of machined joint surfaces 显示文摘 | FU Weiping HUANG Yumei ZHANG Xueliang | 2000 | Journal of Vibration and Acoustics2000，122，4： | 1 |
| 4 | Experimental investigation of dynamic normal characteristics of machined joint surfaces显示文摘 | FU Weiping HUANG YuMei ZHANG GuangPeng | 2000 | Journal of Vibration and Acoustics2000，，10： | 1 |
| 5 | Experimental investigation of dynamic normal characteristics of machined joint surfaces显示文摘 | FU Weiping HUANG Yumei ZHANG Xueliang | 2000 | Journal of Vibration and Acoustics2000，122，4： | 1 |
| 6 | A facile route to synthesis of MoS2 nanorods显示文摘 | Yumei Tian Jingzhe Zhao Wuyou Fu ct al | 2005 | Materials Letters2005，59，： | 1 |
| 7 | Experimental investigation of dynamic normal characteristics of machined joint surfaces显示文摘 | FU Weiping HUANG Yumei ZHANG Xueliang | 2000 | ASME Journal of Vibration and Acoustics2000，122，4： | 1 |
| 8 | Experimental investigation of dynamic normal charac- teristics of machined joint surfaees显示文摘 | Fu Weiping Huang Yumei Zhang Xueliang | 2000 | Transactions of the ASME Journal of Vibration and Aeousties2000，122，4： | 1 |
| 9 | Experimental investigation on damping behavior of normal joint surfaces at unit area显示文摘 | FU Weiping HUANG Yumei ZHANG Guangpeng | 1993 | Modeling Measurement & Control B1993，51，2： | 1 |
| 10 | Activation of the receptor for advanced glycation end products (RAGE) exacerbates experimental autoimmune myasthenia gravis symptoms显示文摘 | Lili Mu Yao Zhang Bo Sun Jinghua Wang Xiaoli Xie Na Li Jia Zhang Qingfei Kong Yumei Liu Zhijuan Han Guangyou Wang Zheng Fu Bo Yu Guozhong Li Hulun Li | 2011 | Clinical Immunology2011，，1： | 1 |
| 11 | Experimental investigation of dynamic normal char- acteristics of machined joint surfaces 显示文摘 | FU Weiping HUANG Yumei ZHANG Xueliang | 2000 | Journal of Vibration and Acoustics2000，122，10： | 1 |
| 12 | Experimental investigation of dynamic normal characteristics of machine joint surfaces 显示文摘 | Fu Weiping Huang Yumei Zhang Guangpeng | 2000 | ASME Journal of Vibration and Acoustics2000，122，4： | 1 |
| 13 | Effect of pyrimorph on soil enzymatic activities and respiration显示文摘 | Dan Xiong Zhenzhen Gao Bin Fu Hu Sun Shuli Tian Yumei Xiao Zhaohai Qin | 2013 | European Journal of Soil Biology2013，，： | 1 |
| 14 | Experimental Investigation on Damping Behavior of Normal Joint Surfaces at Unit Area显示文摘 | Fu Weiping Huang Yumei Zhang Guangpeng | 1993 | Modeling Measurement & Control B1993，51，2： | 1 |
| 15 | Experimental investigation on damping behaviors of normal joint surface at unit area 显示文摘 | Fu Weiping Huang Yumei Zhang Guangpeng | 1993 | Modelling Measurement & Control B1993，51，2： | 1 |
| 16 | De novo synthesis of g|utathione is a prerequisite for curcumin to inhibit hepatic stellate cell (HSC) activation显示文摘 | ZHENG Shizhong FU Yumei CHEN Anping | 2007 | Free Radical Biology and Medicine2007，43，3： | 1 |
| 17 | Curcumin pro- tects the rat liver from CC14-caused injury and fibrogenesis by at- tenuating oxidative stress and suppressing inflammation 显示文摘 | Fu Yumei Zheng Shizhong Lin Jianguo | 2008 | Mol Pharmacol2008，73，： | 1 |
| 18 | Identification and mapping of pm2026: a recessive powdery mildew resistance gene in an einkorn (Triticum monococcum L.) accession显示文摘 | Hongxing Xu Guoqi Yao Li Xiong Lili Yang Yumei Jiang Bisheng Fu Wenfang Zhao Zhengzhi Zhang Caiqin Zhang Zhengqiang Ma | 2008 | Theoretical and Applied Genetics2008，，4： | 1 |
| 19 | Experimental in-vestigation on damping behaviors of normal joint surface at unit area显示文摘 | Fu Weiping Huang Yumei Zhang Guangpeng | 1993 | Modell Meas Control B1993，51，2： | 1 |
| 20 | An ultrapotent pan-β-coronavirus lineage B(β-CoV-B)neutralizing antibody locks the receptor-binding domain in closed conformation by targeting its conserved epitope显示文摘New threats posed by the emerging circulating variants of SARS-CoV-2 highlight the need to find conserved neutralizing epitopes for therapeutic antibodies and efficient vaccine design.Here,we identified a receptorbinding domain(RBD)-binding antibody,XG014,which potently neutralizesβ-coronavirus lineage B(β-CoV-B),including SARS-CoV-2,its circulating variants,SARSCoV and bat SARSr-CoV WIV1.Interestingly,antibody family members competing with XG014 binding show reduced levels of cross-reactivity and induce antibodydependent SARS-CoV-2 spike(S)protein-mediated cellcell fusion,suggesting a unique mode of recognition by XG014.Structural analyses reveal that XG014 recognizes a conserved epitope outside the ACE2 binding site and completely locks RBD in the non-functional“down”conformation,while its family member XG005 directly competes with ACE2 binding and position the RBD“up”.Single administration of XG014 is effective in protection against and therapy of SARS-CoV-2 infection in vivo.Our findings suggest the potential to develop XG014 as pan-β-CoV-B therapeutics and the importance of the XG014 conserved antigenic epitope for designing broadly protective vaccines againstβ-CoV-B and newly emerging SARS-CoV-2 variants of concern. | Zezhong Liu Wei Xu Zhenguo Chen Wangjun Fu Wuqiang Zhan Yidan Gao Jie Zhou Yunjiao Zhou Jianbo Wu Qian Wang Xiang Zhang Aihua Hao Wei Wu Qianqian Zhang Yaming Li Kaiyue Fan Ruihong Chen Qiaochu Jiang Christian TMayer Till Schoofs Youhua Xie Shibo Jiang Yumei Wen Zhenghong Yuan Kang Wang Lu Lu Lei Sun Qiao Wang | 2022 | Protein & Cell2022，13，9： | 1 |
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