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| 1 | MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar显示文摘Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants. | Yamei Wang Wenwen Liu Xinwei Wang Ruijuan Yang Zhenying Wu HanWang LeiWang Zhubing Hu Siyi Guo Hailing Zhang Jinxing Lin Chunxiang Fu | 2020 | Horticulture Research2020，7，1： | 9 |
| 2 | An Efficient and Stable Ionic Liquid System for Synthesis of Ethylene Glycol via Hydrolysis of Ethylene Carbonate显示文摘An ionic liquid system of [Bmim]X/[Bmim]OH(X Cl,BF4,and PF6,) was developed for the hydroly-sis of ethylene carbonate to ethylene glycol. The important parameters,such as the variety of ionic liquids,molar ratio of [Bmim]X to [Bmim]OH,amount of ionic liquid,molar ratio of water to ethylene carbonate,reaction tem-perature,pressure and reaction time,were investigated systematically. Excellent yield(>93%) and high selectivity(99.5%) of ethylene glycol were achieved. Under the optimum reaction conditions,the ionic liquid system could be reused at least five times and the selectivity of ethylene glycol remained higher than 99.5%. | MENG Zhenying SUN Jian WANG Jinquan ZHANG Jianxin FU Zengzeng CHENG Weiguo ZHANG Xiangping | 2010 | Chinese Journal of Chemical Engineering2010，18，6： | 8 |
| 3 | Shale Oil Occurrence and Reservoir Characteristics of the Qijia-Gulong Depression in Songliao Basin显示文摘1 Introduction The technology breakthrough in the exploration of shale gas and tight oil has greatly extended the global fossil fuel resources(Jia et al.,2012;Zou et al.,2012;Qiu et al.,2013).Although shale oil has been the global hot topic in the study of unconventional resources,there are varied definitions with respect to shale oil by different researchers. | HUO Qiuli ZENG Huasen ZHANG Xiaochang FANG Qinghua WANG Zhenying FU Li | 2015 | Acta Geologica Sinica(English Edition)2015，89，A01： | 1 |
| 4 | Overexpression of PvWOX3a in switchgrass promotes stem development and increases plant height显示文摘Switchgrass(Panicum virgatum L.)is an important perennial,noninvasive,tall ornamental grass that adds color and texture to gardens and landscapes.Moreover,switchgrass has been considered a forage and bioenergy crop because of its vigorous growth,low-input requirements,and broad geography.Here,we identified PvWOX3a from switchgrass,which encodes a WUSCHEL-related homeobox transcription factor.Transgenic overexpression of PvWOX3a in switchgrass increased stem length,internode diameter,and leaf blade length and width,all of which contributed to a 95%average increase in dry weight biomass compared with control plants.Yeast one-hybrid and transient dual-luciferase assays showed that PvWOX3a can repress the expression of gibberellin 2-oxidase and cytokinin oxidase/dehydrogenase through apparently direct interaction with their promoter sequences.These results suggested that overexpression of PvWOX3a could increase gibberellin and cytokinin levels in transgenic switchgrass plants,which promotes cell division,elongation,and vascular bundle development.We also overexpressed PvWOX3a in a transgenic miR156-overexpressing switchgrass line that characteristically exhibited more tillers,thinner internodes,and narrower leaf blades.Double transgenic switchgrass plants displayed significant increases in internode length and diameter,leaf blade width,and plant height but retained a tiller number comparable to that of plants expressing miR156 alone.Ultimately,the double transgenic switchgrass plants produced 174%more dry-weight biomass and 162%more solubilized sugars on average than control plants.These findings indicated that PvWOX3a is a viable potential genetic target for engineering improved shoot architecture and biomass yield of horticulture,fodder,and biofuel crops. | Ruijuan Yang Zhenying Wu Chen Bai Zhichao Sun Mengqi Wang Yuzhu Huo Hailing Zhang Yamei Wang Huapeng Zhou Shaojun Dai Wenwen Liu Chunxiang Fu | 2021 | Horticulture Research2021，8，1： | 1 |
| 5 | Thin Layer Identification of Jiedu Shengxue Granules and Determination of Notoginsenoside R1 Content显示文摘[Objectives]To establish the quality standard of hospital preparation Jiedu Shengxue granules.[Methods]Scleromitrion diffusum and Prunella vulgaris in Jiedu Shengxue granules were qualitatively identified by thin layer chromatography(TLC).A high performance liquid chromatography(HPLC)was established to determine the content of notoginsenoside R1 in the granule.[Results]The traditional Chinese medicinal materials in Jiedu Shengxue granules could be identified by TLC,and the characteristic spots were stable and clear.Notoginsenoside R1 had a good linear relationship in the range of 10.45-104.5μg/mL,with an average recovery of 98.52%and RSD=2.36%.[Conclusions]TLC and HPLC,as the quality control methods of Jiedu Shengxue granules,have high accuracy and good repeatability,which lays a foundation for the quality control of this mixture. | Zhenying FU Bing QING Yinghong HUANG Xianyi SHI Meiyan QIU Xian PENG Jiangcun WEI Fengzhen LI Wen ZHONG | 2024 | Medicinal Plant2024，15，1： | 0 |
| 6 | Functional characterization of caffeic acid O-methyltransferase in internode lignification of switchgrass(Panicum virgatum)显示文摘Caffeic acid O-methyltransferase(COMT) is a crucial enzyme that mainly methylates phenylpropanoid meta-hydroxyl of C5 in the biosynthesis of syringyl lignin in angiosperms. A putative COMT, named as PvCOMT1,was isolated from switchgrass(Panicum virgatum), a C4 warm-season dual-purpose forage and bioenergy crop. Our results showed that recombinant PvCOMT1 enzyme protein catalyzed the methylation of 5-OH coniferyl alcohol, 5-OH coniferaldehyde(CAld5H) and 5-OH ferulic acid. Further in vitro studies indicate that CAld5H can dominate COMT-mediated reactions by inhibiting the methylation of the other substrates. Transgenic switchgrass plants generated by an RNAi approach were further employed to study the function of COMT in internode lignification. A dramatic decrease in syringyl lignin units coupled with an obvious incorporation in 5-OH guaiacyl lignin units were observed in the COMT-RNAi transgenic plants. However, the constitutive suppression of COMT in switchgrass plants altered neither the pattern of lignin deposition along the stem nor the anatomical structure of internodes. Consistent with the biochemical characterization of PvCOMT1, a significant decrease in sinapaldehyde was found in the COMT-RNAi transgenic switchgrass plants, suggesting that CAld5H could be the optimal intermediate in the biosynthesis syringyl lignin. | Fengyan WU Zhenying WU Aiguo YANG Shanshan JIANG Zeng-Yu WANG Chunxiang FU | 2018 | Frontiers of Agricultural Science and Engineering2018，5，1： | 0 |
| 7 | Preliminary Study and Quality Analysis of Laggera alata显示文摘[Objectives]To establish a method for quality analysis of Laggera alata.[Methods]The water content,total ash and alcohol-soluble extract of Laggera alata were determined according to the method of 2015 edition of Chinese Pharmacopoeia.[Results]Ten batches of Laggera alata from different producing areas and different collection time in Guangxi had the same plant morphology and medicinal properties.The experimental results were as follows:the water content was 5.19%-10.86%;the total ash content was 5.91%-10.74%;the acid-insoluble ash content was 0.44%-0.92%;the extract content was 14.64%-19.95%.[Conclusions]The experimental results can provide scientific basis for the development and utilization of Laggera alata and the establishment of its quality standard. | Jiangcun WEI Jiabao MA Jian CUI Zhenying FU Xianyi SHI Yan MA Lifu WEI Zhengteng YANG | 2020 | Medicinal Plant2020，11，3： | 0 |
| 8 | Simultaneous Determination of Gallic Acid and Cinnamic Acid Content in Rhubarb by HPLC显示文摘[Objective]The paper was to determine the contents of gallic acid and cinnamic acid in Chinese herb rhubarb by HPLC.[Method]High performance liquid chromatography(HPLC)was established with the following conditions:Thermo SCIENTIFIC Hypersil GOLD Dim column(4.6 mm×250 mm,5μm),mobile phase methanol-0.1%phosphoric acid solution,gradient elution,detection wavelength 278 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10 pL.[Result]The content of gallic acid and cinnamic acid in rhubarb was determined by HPLC,and they showed good linear relationship in the linear ranges of 0.125-1.250 pg(E2=0.9998)and 0.0382-0.3820μg(E2=0.9994),respectively.[Conclusion]A HPLC method is established to determine gallic acid and cinnamic acid content in rhubarb.The method will provide a scientific basis for quality control of rhubarb and its traditional Chinese medicine. | Han Qian Wei Jiangcun Chen Yong Shi Xianyi Wu Yuyan Fu Zhenying Huang Zhouyan Liu Wenjun | 2020 | Plant Diseases and Pests2020，11，3： | 0 |
| 9 | Quality Control of Zhuang Medicine Xiaoyan Zhiyang Lotion显示文摘[Objectives]To establish the TLC identification methods of Llicis Rotundae Cortex and Mahoniae Caulis and the determination method of syringin content in Zhuang Medicine Xiaoyan Zhiyang Lotion.[Methods]Thin layer chromatography(TLC)was used to qualitatively identify Llicis Rotundae Cortex and Mahoniae Caulis in Zhuang Medicine Xiaoyan Zhiyang Lotion.The quantitative analysis of syringin in Zhuang Medicine Xiaoyan Zhiyang Lotion was carried out by high-performance liquid chromatography(HPLC).Chromatographic conditions are as follows:Luna 5μm C_(18)((2)100 A(250 mm×4.6 mm,5μm)column was adopted;acetonitrile(10%)-water(90%)was as the mobile phase,and the flow rate was 1.0 mL/min;the detection wavelength was 265 nm;the sample size was 5μL;the column temperature was 30℃.[Results]Syringin had a good linear relationship with its peak area in the range of 0.190-2.856μg(R^(2)=0.9995).The RSD values of precision,stability and repeatability experiments were all lower than 2.5%,and the average sampling recovery rate(n=6)was 100.37%(RSD=-0.88%).[Conclusions]The method had the advantages of simple operation,strong specificity,good repeatability and stability,and can be used for the quality control of Zhuang Medicine Xiaoyan Zhiyang Lotion. | Xian PENG Zhenying FU Jiangcun WEI Yinghong HUANG Xianyi SHI Wei TIAN Wen ZHONG Guodong HUANG | 2023 | Medicinal Plant2023，14，5： | 0 |
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