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| 1 | Suppression of m6A reader Ythdf2 promotes hematopoietic stem cell expansion显示文摘 | Zhenrui Li Pengxu Qian Wanqing Shao Hailing Shi Xi C. He Madelaine Gogol Zulin Yu Yongfu Wang Meijie Qi Yunfei Zhu John M. Perry Kai Zhang Fang Tao Kun Zhou Deqing Hu Yingli Han Chongbei Zhao Richard Alexander Hanzhang Xu Shiyuan Chen Allison Peak Kathyrn Hall Michael Peterson Anoja Perera Jeffrey S. Haug Tari Parmely Hua Li Bin Shen Julia Zeitlinger Chuan He Linheng Li | 2018 | Cell Research2018，28，9： | 18 |
| 2 | The coupling of dynamics and permeability in the hydrocarbon accumulation period controls the oil-bearing potential of low permeability reservoirs:a case study of the low permeability turbidite reservoirs in the middle part of the third member of Shahejie显示文摘The relationships between permeability and dynamics in hydrocarbon accumulation determine oilbearing potential(the potential oil charge) of low permeability reservoirs.The evolution of porosity and permeability of low permeability turbidite reservoirs of the middle part of the third member of the Shahejie Formation in the Dongying Sag has been investigated by detailed core descriptions,thin section analyses,fluid inclusion analyses,carbon and oxygen isotope analyses,mercury injection,porosity and permeability testing,and basin modeling.The cutoff values for the permeability of the reservoirs in the accumulation period were calculated after detailing the accumulation dynamics and reservoir pore structures,then the distribution pattern of the oil-bearing potential of reservoirs controlled by the matching relationship between dynamics and permeability during the accumulation period were summarized.On the basis of the observed diagenetic features and with regard to the paragenetic sequences,the reservoirs can be subdivided into four types of diagenetic fades.The reservoirs experienced two periods of hydrocarbon accumulation.In the early accumulation period,the reservoirs except for diagenetic fades A had middle to high permeability ranging from 10 × 10^(-3) μm^2 to4207 × 10^(-3) μm^2.In the later accumulation period,the reservoirs except for diagenetic fades C had low permeability ranging from 0.015 × 10^(-3)~ μm^2 to 62 × 10^(-3) μm^2.In the early accumulation period,the fluid pressure increased by the hydrocarbon generation was 1.4-11.3 MPa with an average value of 5.1 MPa.and a surplus pressure of1.8-12.6 MPa with an average value of 6.3 MPa.In the later accumulation period,the fluid pressure increased by the hydrocarbon generation process was 0.7-12.7 MPa with an average value of 5.36 MPa and a surplus pressure of1.3-16.2 MPa with an average value of 6.5 MPa.Even though different types of reservoirs exist,all can form hydrocarbon accumulations in the early accumulation period.Such types of reservoirs can form hydrocarbon accumulation with high accumulation dynamics:however,reservoirs with diagenetic facies A and diagenetic fades B do not develop accumulation conditions with low accumulation dynamics in the late accumulation period for very low permeability.At more than 3000 m burial depth,a larger proportion of turbidite reservoirs are oil charged due to the proximity to the source rock.Also at these depths,lenticular sand bodies can accumulate hydrocarbons.At shallower depths,only the reservoirs with oil-source fault development can accumulate hydrocarbons.For flat surfaces,hydrocarbons have always been accumulated in the reservoirs around the oil-source faults and areas near the center of subsags with high accumulation dynamics. | Tian Yang Ying-Chang Cao Yan-Zhong Wang Henrik Friis Beyene Girma Haile Ke-Lai Xi Hui-Na Zhang | 2016 | Petroleum Science2016，13，2： | 10 |
| 3 | On the Kinematic Characteristics and Dynamic Process of Boundary faults of the Nansha Ultra-crust Layer-Block显示文摘The Nansha ultra-crust layer-block is confined by ultra-crustal boundary faults of distinctive features, bordering the Kangtai-Shuangzi-Xiongnan extensional faulted zone on the north, the Baxian-Baram-Yoca-Cuyo nappe faulted zone on the south, the Wan’an-Natuna strike-slip tensional faulted zone on the west and the Mondoro-Panay strike-slip compressive faulted zone on the east. These faults take the top of the Nansha asthenosphere as their common detachmental surface. The Cenozoic dynamic process of the ultra-crust layer-block can be divided into four stages: K2-E21, during which the northern boundary faults extended, this ultra-crust layer-block was separated from the South China-Indosinian continental margin, the Palaeo-South China Sea subducted southwards and the Sibu accretion wedge was formed; E22-E31, during which the Southwest sub-sea basin extended and orogeny was active due to the collision of the Sibu accretion wedge; E32-N11, during which the central sub-sea basin extended, the Miri | LIU Hailing SUN Yan GUO Lingzhi SHU Liangshu YANG Shukang ZHOU Di ZHANG Yixiang | 1999 | Acta Geologica Sinica(English Edition)1999，73，4： | 9 |
| 4 | 中国近地面臭氧来源解析研究综述显示文摘近年来,在实施了一系列较为严格的空气污染控制措施后,中国的PM2.5污染已得到明显改善,但近地面臭氧污染问题却日益凸显,尤其是在一些发达的城市群,如京津冀,长三角,珠三角和四川盆地地区。鉴于臭氧生成的复杂性及其与前体物的非线性关系,准确定位臭氧及其前体物的来源是制定经济高效的臭氧污染控制策略的重要前提。本文总结了近20年来国内外已发表文章中关于中国臭氧来源(包括区域和行业来源)的主要方法 (包括基于观测的方法和基于排放的方法)和结论,希望能为中国臭氧污染的全面了解和空气污染防治政策的制定提供可靠的参考。 | LIU Hailing ZHANG Meigen HAN Xiao | 2020 | Atmospheric and Oceanic Science Letters2020，13，5： | 9 |
| 5 | MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar显示文摘Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants. | Yamei Wang Wenwen Liu Xinwei Wang Ruijuan Yang Zhenying Wu HanWang LeiWang Zhubing Hu Siyi Guo Hailing Zhang Jinxing Lin Chunxiang Fu | 2020 | Horticulture Research2020，7，1： | 9 |
| 6 | Influence of Low Load Truncation Level on Crack Growth for Al 2324-T39 and Al 7050-T7451显示文摘Tests with middle-crack tension(M(T)) specimens made of Al 2324-T39 and Al 7050-T7451 are conducted to investigate the influence of low load truncation level on fatigue crack growth.The six different truncated spectra are obtained by removing the small cycles of which amplitudes are less than the specified percentages of the maximum amplitude in the basic flight-by-flight loading spectrum and the remainder of the spectrum is untouched.The tests indicate that the mean level of fatigue crack growth life(FCGL) increases as the load truncation level is enhanced.Considering both the time saving and the influence on FCGL,there is an applicable choice(i.e.spectrum S2 or spectrum S3 in this investigation) for full scale fatigue test.The scatter of FCGL becomes much larger than that under the basic spectrum when the load truncation level is increased to a specified high level,mainly due to the occurrence of crack slanting and branching under the high level truncated loading spectra. | Tian Hailing Bao Rui Zhang Jianyu Zheng Xiaoling Fei Binjun | 2009 | Chinese Journal of Aeronautics2009，22，4： | 8 |
| 7 | Bacillus cereus AR156 primes induced systemic resistance by suppressing miR825/825 and activating defense-related genes in Arabidopsis显示文摘Small RNAs play an important role in plant immune responses. However, their regulatory function in induced systemic resistance(ISR) is nascent. Bacillus cereus AR156 is a plant growth-promoting rhizobacterium that induces ISR in Arabidopsis against bacterial infection. Here,by comparing small RNA profiles of Pseudomonas syringae pv. tomato(Pst) DC3000-infected Arabidopsis with and without AR156 pretreatment, we identified a group of Arabidopsis micro RNAs(mi RNAs) that are differentially regulated by AR156 pretreatment. mi R825 and mi R825 are two mi RNA generated from a single mi RNA gene.Northern blot analysis indicated that they were significantly downregulated in Pst DC3000-infected plants pretreated with AR156, in contrast to the plants without AR156 pretreatment. mi R825 targets two ubiquitin-protein ligases,while mi R825 targets toll-interleukin-like receptor(TIR)-nucleotide binding site(NBS) and leucine-rich repeat(LRR)type resistance(R) genes. The expression of these target genes negatively correlated with the expression of mi R825 and mi R825. Moreover, transgenic plants showing reduced expression of mi R825 and mi R825 displayed enhanced resistance to Pst DC3000 infection, whereas transgenic plants overexpressing mi R825 and mi R825 were more susceptible. Taken together, our data indicates that Bacillus cereus AR156 pretreatment primes ISR to Pst infection by suppressing mi R825 and mi R825 and activating the defense related genes they targeted. | Dongdong Niu Jing Xia Chunhao Jiang Beibei Qi Xiaoyu Ling Siyuan Lin Weixiong Zhang Jianhua Guo Hailing Jin Hongwei Zhao | 2016 | Journal of Integrative Plant Biology2016，58，4： | 6 |
| 8 | Numerical analysis and simulation of Czochralski growth processes for large diameter silicon crystals显示文摘Numerical analysis and simulation have been an effective means to develop the advanced growth technology and to control the defects type, size and density for silicon crystals of 300 mm and beyond. In the present paper, numerical analysis of the melt flow in the Czochralski (CZ) crystal growth configuration, the three dimensional (3D) modeling, the simulation of melt flow under the magnetic field, the inverse modeling and the time-dependent simulation are reviewed. Fi nally, comparison of numerical analysis with experimental measurements is discussed. | TU Hailing XIAO Qinghua GAO Yu ZHOU Qigang ZHANG Guohu CHANG Qing | 2007 | Rare Metals2007，26，6： | 5 |
| 9 | Effect of defect complex on magnetic properties of (Fe, Mn)-doped ZnO thin films显示文摘We have observed room temperature ferromagnetism in Mn-doped and (Fe, Mn)-codoped ZnO thin films grown under different oxygen partial pressures by pulsed laser deposition. The X-ray diffraction and optical transmission spectra studies demonstrate the natural incorporation of Fe and Mn cations into wurtzite ZnO lattices. The effects of transition metal doping and defects on the magnetic properties was investigated. It is found that room temperature ferromagnetism is sensitive to oxygen vacancy and Zn vacancy. The absence of ferromagnetism in pure ZnO films grown under different oxygen partial pressures reveals that the transition metal ions should also play an important role in inducing the ferromagnetism. | Yang, Hailing Xu, Xiaoguang Zhang, Guoqing Miao, Jun Zhang, Xin Wu, Shizhe Jiang, Yong | 2012 | Rare Metals2012，31，2： | 5 |
| 10 | Evaluation of Spectral Scale Effects in Estimation of Vegetation Leaf Area Index Using Spectral Indices Methods显示文摘Spectral index methodology has been widely used in Leaf Area Index(LAI) retrieval at different spatial scales. There are differences in the spectral response of different remote sensors and thus spectral scale effect generated during the use of spectral indices to retrieve LAI. In this study, PROSPECT, leaf optical properties model and Scattering by Arbitrarily Inclined Layers(SAIL) model, were used to simulate canopy spectral reflectance with a bandwidth of 5 nm and a Gaussian spectral response function was employed to simulate the spectral data at six bandwidths ranging from 10 to 35 nm. Additionally, for bandwidths from 5 to 35 nm, the correlation between the spectral index and LAI, and the sensitivities of the spectral index to changes in LAI and bandwidth were analyzed. Finally, the reflectance data at six bandwidths ranging from 40 to 65 nm were used to verify the spectral scale effect generated during the use of the spectral index to retrieve LAI. Results indicate that Vegetation Index of the Universal Pattern Decomposition(VIUPD) had the highest accuracy during LAI retrieval. Followed by Normalized Difference Vegetation Index(NDVI), Modified Simple Ratio Indices(MSRI) and Triangle Vegetation Index(TVI), although the coefficient of determination R^2 was higher than 0.96, the retrieved LAI values were less than the actual value and thus lacked validity. Other spectral indices were significantly affected by the spectral scale effect with poor retrieval results. In this study, VIUPD, which exhibited a relatively good correlation and sensitivity to LAI, was less affected by the spectral scale effect and had a relatively good retrieval capability. This conclusion supports a purported feature independent of the sensor of this model and also confirms the great potential of VIUPD for retrieval of physicochemical parameters of vegetation using multi-source remote sensing data. | DU Huishi JIANG Hailing ZHANG Lifu MAO Dehua WANG Zongming | 2016 | Chinese Geographical Science2016，26，6： | 5 |
| 11 | Secondary Solidification Behaviour of AA8006 Alloy Prepared by Suction Casting显示文摘Solidification behaviour of AA8006 aluminium alloy in suction casting has been investigated by field emission scanning electron microscopy with energy dispersive spectroscopy (EDS). It is found that there is a secondary solidification process of the remaining liquid in located region of α-Al dendrites, and the cooling rate in?uences not only the solidification of the primary α-Al dendrite, but also the secondary solidification process of the remaining liquid. With the primary solidification being responsible for the formation of the relatively large α- Al dendrite, a fine and homogeneous microstructure is observed in the secondary solidification. Furthermore, because of the presence of the fine microstructure, the eutectic reaction is confined into small intergranular areas, inducing the formation of the laminar eutectic phase in the primary solidification region and fibrous eutectic phase in the secondary solidification region. EDS analysis shows that the content of Fe is higher in the secondary solidification region, and the enrichment of the solute element further confirms the existence of the secondary solidification. | Zhongwei Chen Hailing Zhang Yimin Lei | 2011 | Journal of Materials Science & Technology2011，27，9： | 5 |
| 12 | Enhancement of transfection efficiency for HeLa cells via incorporating arginine moiety into chitosan显示文摘Arginine-rich peptides have attracted considerable attention due to their distinct internalization mechanism. It was reported that arginine and guanidino moieties were able to translocate through cell membranes and played a critical role in the process of membrane permeation. In this work, arginine was conjugated to the backbone of chitosan to form a novel chitosan derivative, arginine modified chitosan (Arg-CS). Arg-CS/DNA complexes were prepared according to the method of coacervation process. The physicochemical properties of Arg-CS and Arg-CS/DNA complexes were characterized and the transfection activity and efficiency mediated by Arg-CS/DNA complexes were investigated taking HeLa cells as target cells. Arg-CS was characterized by FTIR and 13C NMR. Arg-CS/DNA polye- lectrolyte complexes were investigated by agarose gel retardation, dynamic light scattering (DLS) and atomic force microscopy (AFM). The results revealed that the Arg-CS/DNA complexes started to form at N/P ratio of 2:1, and the size of particles varied from 100 to 180 nm. The cytotoxicity of Arg-CS and their complexes with plasmid DNA were determined by MTT assay for HeLa cells, and the results suggested that Arg-CS/DNA complexes were slightly less toxic than Arg-CS. Moreover, the derivative alone and their complexes showed significantly lower toxicity than PEI and PEI/DNA complexes, respectively. Taking HeLa cells as target cells and using pGL3-control as reporter gene, the luciferase expression mediated by Arg-CS was greatly enhanced to about 100 folds compared with the luciferase expression mediated by chitosan at different pH media. These results suggest that Arg-CS is a promising candi- date as a safe and efficient vector for gene delivery and transfection. | ZHU DunWan ZHANG HaiLing BAI JinGen LIU WenGuang LENG XiGang SONG CunXian YANG Jian LI XiaoWei JIN Xu SONG LiPing LIU LanXia LI XiuLan ZHANG Yang YAO KangDe | 2007 | Chinese Science Bulletin2007，52，23： | 4 |
| 13 | NS1-binding protein radiosensitizes esophageal squamous cell carcinoma by transcriptionally suppressing c-Myc显示文摘Background:Cisplatin-based chemotherapy with concurrent radiotherapy is a standard treatment for advanced esophageal squamous cell carcinoma(ESCC).NS1-binding protein(NS1-BP),a member of the BTB-kelch protein fam-ily,has been shown to inhibit the proliferation of Hela cells by suppressing c-Myc.In the present study,we examined the potential function role of NS1-BP expression in ESCC,and particularly,the sensitivity of ESCC to radiotherapy.Methods:NS1-BP expression was examined using immunohistochemistry in two cohorts(n=98 for the training cohort;n=46 for independent validation cohort)of ESCC patients receiving cisplatin-based chemotherapy and con-current radiotherapy.Normal esophageal mucosal tissue blocks were used as a control.We also conducted a series of in vitro and in vivo experiments to examine the potential effects of over-expressing NS1-BP on ESCC cells,and particu-larly their sensitivity to ionizing irradiation.Results:In the training cohort,NS1-BP downregulation was observed in 59%(85/144)of the ESCC specimens.NS1-BP downregulation was associated with chemoradiotherapeutic resistance and shorter disease-specific survival(DSS)in both the training and validation cohorts.Over-expressing NS1-BP in cultured ESCC cells substantially increased the cellular response to irradiation both in vitro and in vivo.NS1-BP also significantly enhanced IR-induced apoptosis,and abrogated IR-induced G2/M cell-cycle arrest and ATM/Chk1 phosphorylation.Immunoprecipitation assays indicated that NS1-BP could interact with c-Myc promoter regions to inhibit its transcription.In ESCC tissues,c-Myc expression was inversely correlated with NS1-BP levels,and was associated with a shorter DSS.Conclusions:Our findings highlight the role and importance of NS1-BP in radiosensitivity of ESCC.Targeting the NS1-BP/c-Myc pathway may provide a novel therapeutic strategy for ESCC. | Yuwen Wang Jingjing Cheng Dan Xie Xiaofeng Ding Hailing Hou Xi Chen Puchun Er Furong Zhang Lujun Zhao Zhiyong Yuan Qingsong Pang Ping Wang Dong Qian | 2018 | Cancer Communications2018，38，1： | 4 |
| 14 | Comparison of Clinical and Epidemiological Characteristics of Asymptomatic and Symptomatic SARS-CoV-2 Infection in Children显示文摘To understand the epidemiological and clinical features of the symptomatic and asymptomatic pediatric cases of COVID-19,we carried out a prospective study in Shanghai during the period of January 19 to April 30,2020.A total of 49 children(mean age 11.5±5.12 years)confirmed with SARS-CoV-2 infection were enrolled in the study,including 11(22.4%)domestic cases and 38(77.6%)imported cases.Nine(81.8%)local cases and 12(31.6%)imported cases had a definitive epidemiological exposure.Twenty-eight(57.1%)were symptomatic and 21(42.9%)were asymptomatic.Neither asymptomatic nor symptomatic cases progressed to severe diseases.The mean duration of viral shedding for SARS-CoV-2 in upper respiratory tract was 14.1±6.4 days in asymptomatic cases and 14.8±8.4 days in symptomatic cases(P>0.05).Forty-five(91.8%)cases had viral RNA detected in stool.The mean duration of viral shedding in stool was 28.1±13.3 days in asymptomatic cases and 30.8±18.6 days in symptomatic participants(P>0.05).Children<7 years shed viral RNA in stool for a longer duration than school-aged children(P<0.05).Forty-three(87.8%)cases had seropositivity for antibodies against SARS-CoV-2 within 1–3 weeks after confirmation with infection.In conclusion,asymptomatic SARS-CoV-2 infection may be common in children in the community during the COVID-19 pandemic wave.Asymptomatic cases shed viral RNA in a similar pattern as symptomatic cases do.It is of particular concern that asymptomatic individuals are potentially seed transmission of SARS-CoV-2 and pose a challenge to disease control. | Jiehao Cai Xiangshi Wang Jun Zhao Yanling Ge Jin Xu He Tian Hailing Chang Aimei Xia Jiali Wang Jinqiang Zhang Zhongqiu Wei Jingjing Li Chuning Wang Jianshe Wang Qirong Zhu Xiaowen Zhai Mei Zeng | 2020 | Virologica Sinica2020，35，6： | 4 |
| 15 | Variant rs2237892 of KCNQ1 Is Potentially Associated with Hypertension and Macrovascular Complications in Type 2 Diabetes Mellitus in A Chinese Han Population显示文摘KCNQ1 has been identified as a susceptibility gene of type 2 diabetes mellitus(T2DM) in Asian populations through genome-wide association studies. However, studies on the association between gene polymorphism of KCNQ1 and T2 DM complications remain unclear. To further analyze the association between different alleles at the single nucleotide polymorphism(SNP) rs2237892 within KCNQ1 and TD2 M and its complications, we conducted a case-control study in a Chinese Han population. The C allele of rs2237892 variant contributed to susceptibility to T2DM(odds ratio [OR], 1.45; 95% confidence interval [CI], 1.20–1.75). Genotypes CT(OR, 1.97; 95% CI,1.24–3.15) and CC(OR, 2.49; 95% CI, 1.57–3.95) were associated with an increased risk of T2 DM. Multivariate regression analysis was performed with adjustment of age, gender, and body mass index. We found that systolic blood pressure(P = 0.015), prevalence of hypertension(P = 0.037), and risk of macrovascular disease(OR, 2.10; CI, 1.00–4.45) were significantly higher in subjects with the CC genotype than in the combined population with genotype either CT | Wanlin Zhang Hailing Wang Xiaomin Guan Qing Niu Wei Li | 2015 | Genomics, Proteomics & Bioinformatics2015，13，6： | 4 |
| 16 | Effect of annealing atmosphere on magnetic properties of pure ZnO and Na:ZnO films显示文摘Pure ZnO and Zn0.96Na0.04O films were grown on quartz substrates by sol-gel technology.The XRD analysis revealed that all thin films had hexagonal wurtzite structure and obvious c-axis preferred orientation.Ferromagnetism was precisely measured by an alternating gradient magnetometer (AGM).To explore the nature original ferromagnetism,the effect of annealing atmosphere on magnetic properties of the films was studied.Compared with pure ZnO,magnetic hysteresis loops showed that doping Na atoms enhanced saturation magnetism.The mag-netism of the films annealed in the air atmosphere was significantly better than that in the O2 atmosphere.The photoluminescence (PL) spec-trum analysis suggested that the ferromagnetism was due to the defects in the films. | Wu, Kai Xu, Xiaoguang Yang, Hailing Zhang, Jianli Miao, Jun Jiang, Yong | 2012 | Rare Metals2012，31，1： | 3 |
| 17 | Synthesis,Characterization and Photocatalytic Activity of TiO_2 Film/Bi_2O_3 Microgrid Heterojunction显示文摘TiO2 film modified by Bi2O3 microgrid array was successfully fabricated by using a microsphere lithography method.The structure and morphology of TiO2 film,Bi2O3 film and TiO2 film/Bi2O3 microgrid heterojunction were characterized through X-ray diffraction,atomic force microscopy and scanning electron microscopy.The optical transmittance spectra and the photocatalytic degradation capacity of these samples to rhodamine B were determined via ultraviolet-visible spectroscopy.The results indicated that the coupled system showed higher photocatalytic activity than pure TiO2 and Bi2O3 films under xenon lamp irradiation.The enhancement of the photocatalytic activity was ascribed to the special structure,which could improve the separation of photo-generated electrons and holes,enlarge the surface area and extend the response range of TiO2 film from ultraviolet to visible region. | Liugang Wang Junying Zhang Chunzhi Li Hailing Zhu Wenwen Wang Tianmin Wang | 2011 | Journal of Materials Science & Technology2011，27，1： | 3 |
| 18 | NS1结合蛋白通过转录抑制c-Myc增强食管鳞状细胞癌的放疗敏感性显示文摘背景与目的基于顺铂的化疗联合放疗是晚期食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)的标准疗法。NS1结合蛋白(NS1-binding protein,NS1-BP)是BTB-kelch蛋白家族中的一员,已被证明可通过抑制c-Myc进而抑制HeLa细胞增殖。在本研究中,我们检测NS1-BP表达在ESCC中的潜在作用,特别是其在ESCC放疗敏感性方面的作用。方法对于两组接受基于顺铂化疗且联合放疗的ESCC患者(研究组n=98;验证组n=46),采用免疫组化方法分别检测NS1-BP表达。使用正常食管黏膜组织块作为对照。我们也进行了一系列体外和体内实验来检测过表达NS1-BP对ESCC细胞各方面的潜在影响,尤其关注的是对电离辐射的敏感性方面的影响。结果在研究组中,59%(85/144)的ESCC样本观察到了NS1-BP下调。在研究组和验证组中,NS1-BP下调与放化疗抵抗和较短的疾病特异生存期(disease-specific survival,DSS)均具有相关性。体内和体外培养条件下过表达NS1-BP均能大幅度提高ECSS细胞对放疗的敏感性。NS1-BP也显著增强IR诱导的凋亡,阻断IR诱导的G2/M细胞周期阻滞和ATM/Chk1磷酸化。免疫沉淀反应显示NS1-BP可以与c-Myc启动子区相互作用从而抑制其转录。在ESCC组织中,c-Myc表达与NS1-BP水平负相关,与较短的疾病特异生存期相关。结论我们的研究结果显示了NS1-BP在ESCC放疗敏感性中的作用和重要性。以NS1-BP/c-Myc通路为靶标有望开发一种新的ESCC治疗策略。 | Yuwen Wang Jingjing Cheng Dan Xie Xiaofeng Ding Hailing Hou Xi Chen Puchun Er Furong Zhang Lujun Zhao Zhiyong Yuan Qingsong Pang Ping Wang Dong Qian | 2019 | 癌症2019，38，2： | 2 |
| 19 | Enhanced catalysis of ultrasmall Au-MoS_2 clusters against reactive oxygen species for radiation protection显示文摘Ionizing radiation produces excessive reactive oxygen species(ROS) which impose detrimental effects on biological systems. Thus, it is important to explore clinically safe and efficacious radioprotection agents to scavenge ROS and reduce the risks of radiotherapy. Recently, emerging catalytic nanomaterials such as sulfide nanomaterials have shown capability of clearing ROS in vivo by unique electron transfers between atoms, but their catalytic activities are yet suboptimal. As such, there is an unmet need to improve catalytic properties for stronger antioxidant activities and radiation protection. Herein, we prepared ultrasmall Au-MoS_2 clusters(~2.5 nm) and they showed enhanced catalytic properties via gold intercalation facilitating increased active sites and synergistic effects. Electrocatalysis results revealed that the catalytic activity of Au-MoS_2 towards H2O_2 was superior to ultrasmall MoS_2 without Au. As a result, we found that improving the electrocatalytic property of Au-MoS_2 can effectively enhance corresponding antioxidant activities and radioprotection effects in vivo. In addition, Au-MoS_2 also showed significant radioprotection in vitro and dramatically reduced the excess of radiation-induced adverse ROS. It also rescued radiation-induced DNA damages and protected the bone marrow hematopoietic system from ionizing radiation. | Peixian Bian Jinxuan Zhang Junying Wang Jiang Yang Jingya Wang Haile Liu Yuanming Sun Meixian Li Xiao-Dong Zhang | 2018 | Science Bulletin2018，63，14： | 2 |
| 20 | Study of the aerostatic and aerodynamic stability of super long-span cable-stayed bridges显示文摘With the increase of span length,the bridge tends to be more flexible,and the wind stability becomes an important problem for the design and construction of super long-span cable-stayed bridges. By taking a super long-span cable-stayed bridge with a main span of 1 400 m as example,the aerostatic and aerodynamic stability of the bridge are investigated by three-dimensional nonlinear aerostatic and aerodynamic stability analysis,and the results are compared with those of a suspension bridge with a main span of 1 385 m,and from the aspect of wind stability,the feasibility of using cable-stayed bridge in super long-span bridge with a main span above 1 000 m is discussed. In addition,the influences of design parameters including the depth and width of the girder,the tower structure,the tower height-to-span ratio,the side-to-main span ratio,the auxiliary piers in the side span and the anchorage system of stay cables,etc on the aerostatic and aerodynamic stability of super long-span cable-stayed bridges are investigated numerically;the key design parameters are pointed out,and also their reasonable values are proposed. | Zhang Xinjun Sun Hailing | 2014 | Engineering Sciences2014，12，2： | 2 |
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