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| 1 | Octreotide inhibits proliferation and induces apoptosis of hepatocellular carcinoma cells显示文摘AIM: To study the effect of octreotide on cell proliferation and apoptosis in different hepatocellular carcinoma (HCC) cells and hepatocytes. METHODS: The proliferation of HCC cells (HepG2, SMMC-7721) and hepatocytes (L-02) was determined by MTT assay. Apoptosis was detected either by fluorescent staining, transmission electron microscopy or flow cytometry. The content of AFP in the supernatant of cultured HCC cells was determined by electrochemiluminescence immunoassay. The expression of SSTR subtypes was identified by RT-PCR. RESULTS: The proliferation of HCC cells and L-02 cells was inhibited significantly by octreotide (0.25, 0.5, 1.0, 2.0 and 4.0 mg/L). However, the apoptosis of HCC cells markedly increased in a concentration-dependent manner. Both the apoptosis index and the percentage of apoptotic cells in L-02 cells were significantly lower than those of HepG2 and SMMC-7721 cells. The content of AFP in the supernatant of cultured HepG2 cells treated with octreotide was also statistically reduced. Furthermore, SSTR2 and SSTR4 were positive in both the hepatocellular carcinoma cells and in the L-02 cells. SSTR3 was only expressed in the two heptatocellular carcinoma cells, and SSTR5 was found in the SMMC-7721 cells. No SSTR1 was detected either in HCC cells or L-02 cells. CONCLUSIONS: Apoptosis induction is a major mechanism of octreotide inhibition on hepatocellular cells. SSTR3 is expressed in the HCC cells, but not in the L-02 cells, which suggests a molecular basis for the HCC-selective effects of octreotide. | Hai-linLIU LiHUO LeiWANG | 2004 | Acta Pharmacologica Sinica2004，25，10： | 15 |
| 2 | Identification and characterization of neuroblasts in the subventricular zone and rostral migratory stream of the adult human brain显示文摘在成年人的前脑遗体在 subventricular 地区(SVZ ) 和象小径一样的喙的迁移的溪流(RMS ) 的存在在神经发生的成年人的大脑,而是坚持识别新神经原兴趣大高度争论。在现在的学习,我们在成年猴子,胎儿的人和成年人的大脑描述了 RMS 的一般配置。我们提供神经母细胞在成年人的大脑的前面的腹的 SVZ 和 RMS 连续地存在的证据。没有形成链,神经母细胞单身地或在对出现;他们展出迁移的形态学和合作快车不成熟的 neuronal 标记 doublecortin, polysialylated 神经房间粘附分子和 β III 导管素。很少这些神经母细胞看起来活跃地在前面的腹的 SVZ 正在增殖,显示沿着 RMS 散布的神经母细胞是最可能的但是任何一个都没在 RMS 源于腹的 SVZ。有趣地,没有神经母细胞在成年人的嗅觉的灯泡被发现。一起拿,我们的数据建议 SVZ 维持能力在成年人的大脑生产神经母细胞。 | CongminWang FangLiu Ying-YingLiu Cai-HongZhao YanYou LeiWang JingxiaoZhang BinWei TongMa QiangqiangZhang YueZhang RuiChen HongjunSong ZhengangYang | 2011 | Cell Research2011，21，11： | 13 |
| 3 | MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar显示文摘Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants. | Yamei Wang Wenwen Liu Xinwei Wang Ruijuan Yang Zhenying Wu HanWang LeiWang Zhubing Hu Siyi Guo Hailing Zhang Jinxing Lin Chunxiang Fu | 2020 | Horticulture Research2020，7，1： | 9 |
| 4 | Provisioning QoS Guarantee by Multipath Routing and Reservation in Ad Hoc Networks显示文摘In this paper, a QoS multipath source routing protocol (QoS-MSR) is proposed for ad hoc networks.It can collect QoS information through route discovery mechanism of multipath source routing (MSR) and establish QoS route with reserved bandwidth. In order to reserve bandwidth efficiently, a bandwidth reservation approach called the multipath bandwidth splitting reservation (MBSR) is presented, under which the overall bandwidth request is split into several smaller bandwidth requests among multiple paths. In simulations, the anthors introduce Insignia, an in-bind signaling system that supports QoS in ad hoc networks, and extend it to multipath Insignia (M-Insignia) with QoS-MSR and MBSR. The results show that QoS-MSR routing protocol with the MBSR algorithm can improve the call admission ratio of QoS traffic, the packet delivery ratio, and the end-to-end delay of both best-effort traffic and QoS traffic. Therefore, QoS-MSR with MBSR is an efficient mechanism that supports QoS for ad hoc networks. | Yan-TaiShu Guang-HongWang LeiWang OliverW.W.Yang Yong-JieFan | 2004 | Journal of Computer Science & Technology2004，19，2： | 6 |
| 5 | DIAGNOSIS AND TREATMENT OF MALIGNANT PANCREATICENDOCRINE TUMOUR显示文摘Objective To summarize our experience in the diagnosis and treatment of malignant pancreatic endocrine tumour. Methods We retrospectively reviewed 36 cases of malignant pancreatic endocrine tumours in our hospital from July 1987 to April 2002, and summarized its clinical features. Results Liver metastasis was the main malignant manifestation of malignant pancreatic endocrine tumours (incidence rate 72.2%). Removals of primary lesion and isolated hepatic metastatic lesion were means of curative therapy. Interventional chemotherapy was an important adjuvant treatment. Conclusion Comprehensive therapy plays an important role in improving the prognosis of malignant pancreatic endoc-rine tumour. | LeiWang Yu-peiZhao Chia-ILee QuanLiao | 2004 | Chinese Medical Sciences Journal2004，19，2： | 4 |
| 6 | Blue revolution for food security under carbon neutrality: A case from the water-saving and drought-resistance rice显示文摘The increasing concentration of greenhouse gases(GHGs)in Earth's atmosphere leads to global warming,which further causes a series of climate changes and does great harm to both human society and natural ecosystems.Agricultural GHG emissions,mainly in theform of methane(CH4)and nitrous oxide(N2O),areasignificantsourceofGHGs,accountingfor~14%total global GHGs(Zhang et al.,2022).One major source of agricultural GHGs is CH4 emissions from rice paddies,which is responsiblefor~10%-12%ofhuman-inducedCH4emissions(van Groenigen et al.,2013)and contributes~2.40%to the enhanced global warming effect(Zhang et al.,2022).The global warming potential of GHGs emissions from rice systems is roughly four times higher than either wheat or maize(Linquist et al.,2012). | Hui Xia Xianxian Zhang Yi Liu Junguo Bi Xiaosong Ma Anning Zhang Hongyan Liu Liang Chen Sheng Zhou Huan Gao Kai Xu Haibin Wei Guolan Liu Feiming Wang Hongyang Zhao Xingxing Luo Danping Hou Qiaojun Lou Fangjun Feng Liguo Zhou Shoujun Chen Ming Yan Tianfei Li Mingshou Li LeiWang Zaochang Liu Xinqiao Yu HanweiMei Lijun Luo | 2022 | Molecular Plant2022，15，9： | 3 |
| 7 | Seasonal variation and sources of atmospheric polycyclic aromatic hydrocarbons in a background site on the Tibetan Plateau显示文摘The study of atmospheric polycyclic aromatic hydrocarbons(PAHs) in northeastern Tibetan Plateau with fragile ecological environment and complex atmospheric circulation system is blank. To understand the characteristics and sources of persistent organic pollutants in the atmosphere of the northeastern Tibetan Plateau, we monitored levels in the central Qilian Mountain. From 2016 to 2017, we collected 45-pair(particle + gas) samples using active air samplers to investigate the sources, transport paths, and their influencing factors.Sources of PAHs were analysed with a source diagnostic model, and atmospheric transport paths were calculated. The concentration range for Σ15PAHs was 439–4666 pg/m^(3), and the average was 2015 pg/m^(3). The PAHs in central Qilian Mountain are mainly low molecular weight(LMW) PAHs. Winter concentrations of PAHs were higher than those in summer. The transport of PAHs is mainly affected by westerlies, and there are seasonal differences. Source analysis showed that PAHs mainly came from coal and biomass combustion and vehicle emissions, with seasonal differences. This study clarifies the concentration and seasonal variation of PAHs in the northern Tibetan Plateau, which is conducive to understanding the atmospheric transport process and fate of pollutants. The background site of Qilian Mountains located in the Silk Road economic belt has the value and significance of longterm observation of pollutants. | Xuemei Wu Weijun Sun Baojuan Huai LeiWang Chuntan Han Yetang Wang Wenying Mi | 2023 | Journal of Environmental Sciences2023，，3： | 2 |
| 8 | Multienzyme co-immobilization-based bioelectrode:Design of principles and bioelectrochemical applications显示文摘Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed. | Ranran Wu Haiyan Song Yuanming Wang LeiWang Zhiguang Zhu | 2020 | Chinese Journal of Chemical Engineering2020，28，8： | 2 |
| 9 | “Bottom‐Up” Embedding of the J?rgensen–Hayashi Catalyst into a Chiral Porous Polymer for Highly Efficient Heterogeneous Asymmetric Organocatalysis显示文摘 | Chang AnWang Zhi KunZhang TaoYue Ya LeiSun LeiWang Wei DavidWang YuanZhang ChongLiu WeiWang | 2012 | Chem. Eur. J2012，，22： | 1 |
| 10 | A high-precision positioning servo controller based on phase/frequency detecting technique of two-phase-type PLL显示文摘 | Takashi Emura LeiWang | 2000 | IEEE Transactions on Industrial Electronics2000，47，6： | 1 |
| 11 | Ischemic rat brain extracts induce human marrow stromal cell growth factor production显示文摘 | XiaoguangChen YiLi LeiWang MarkKatakowski LijieZhang JieliChen YongxianXu Subhash C.Gautam MichaelChopp | 2003 | Neuropathology2003，，4： | 1 |
| 12 | Intravenous bone marrow stromal cell therapy reduces apoptosis and promotes endogenous cell proliferation after stroke in female rat显示文摘 | JieliChen YiLi MarkKatakowski XiaoguangChen LeiWang DunyueLu MeiLu Subhash C.Gautam MichaelChopp | 2003 | J Neurosci Res2003，，6： | 1 |
| 13 | Chemokine ligand 2 (CCL2) induces migration and differentiation of subventricular zone cells after stroke显示文摘 | Xian ShuangLiu Zheng GangZhang Rui LanZhang Sara R.Gregg LeiWang TohYier MichaelChopp | 2007 | J Neurosci Res2007，，10： | 1 |
| 14 | Dauricine induces apoptosis, inhibits proliferation and invasion through inhibiting NF‐κB signaling pathway in colon cancer cells显示文摘 | ZhengfengYang ChenghaiLi XiuWang ChunyanZhai ZhengfangYi LeiWang BishengLiu BingDu HuihuiWu XizhiGuo MingyaoLiu DaliLi JianLuo | 2010 | J Cell Physiol2010，，1： | 1 |
| 15 | Moderating effect of Zhong Yong on the relationship between creativity and innovation behaviour显示文摘 | XiangYao QianYang NiniDong LeiWang | 2010 | Asian Journal of Social Psychology2010，，1： | 1 |
| 16 | Quantitative proteomics analysis of parthenogenetically induced pluripotent stem cells显示文摘Parthenogenetic embryonic stem(pES)cells isolated from parthenogenetic activation of oocytes and embryos,also called parthenogenetically induced pluripotent stem cells,exhibit pluripotency evidenced by both in vitro and in vivo differentiation potential.Differential proteomic analysis was performed using differential in-gel electrophoresis and isotope-coded affinity tag-based quantitative proteomics to investigate the molecular mechanisms underlying the developmental pluripotency of pES cells and to compare the protein expression of pES cells generated from either the in vivo-matured ovulated(IVO)oocytes or from the in vitro-matured(IVM)oocytes with that of fertilized embryonic stem(fES)cells derived from fertilized embryos.A total of 76 proteins were upregulated and 16 proteins were downregulated in the IVM pES cells,whereas 91 proteins were upregulated and 9 were downregulated in the IVO pES cells based on a minimal 1.5-fold change as the cutoff value.No distinct pathways were found in the differentially expressed proteins except for those involved in metabolism and physiological processes.Notably,no differences were found in the protein expression of imprinted genes between the pES and fES cells,suggesting that genomic imprinting can be corrected in the pES cells at least at the early passages.The germline competent IVM pES cells may be applicable for germ cell renewal in aging ovaries if oocytes are retrieved at a younger age. | Zhe Hu LeiWang Zhensheng Xie Xinlei Zhang Du Feng Fang Wang Bingfeng Zuo Lingling Wang Zhong Liu Zhisheng Chen Fuquan Yang Lin Liu | 2011 | Protein & Cell2011，2，8： | 0 |
| 17 | 在Ad Hoc网络中利用多径路由和预留提供服务质量保证显示文摘无线通信技术和计算机网络技术的发展为无线移动Ad Hoc网络的产生奠定了基础。Ad Hoc网络,不论是军用还是民用都有着广泛的应用价值和重要的研究意义。在Ad Hoe网络研究中,因为每个节点兼具路由器的功能,所以路由是一个非常重要的研究问题。传送具有QoS保证的业务也是Ad Hoc网络需要解决的问题之一。这对于带宽较低、通信线路质量受限的Ad Hoc网络而言,是很大的挑战。所以,如何在Ad Hoc网络条件下合理地利用无线网络资源,实现有针对性的QoS保证也具有现实意义。 | Yan-TaiShu Guang-HongWang LeiWang OliverW.W.Yang Yong-JieFan | 2004 | Journal of Computer Science & Technology2004，19，C00： | 0 |
| 18 | Nickel Chloride Promoted Glaser Coupling Reaction in Hot Water显示文摘A Glaser coupling reaction of terminal alkynes in the presence of nickel chloride without any organics and bases in hot water has been developed, which produces the corresponding homo-coupling products in good yields. | PinHuaLI LeiWANG MinWANG JinCanYAN | 2004 | Chinese Chemical Letters2004，15，11： | 0 |
| 19 | A Ferrocenylsulfide-mediated Sulfur Ylide Epoxidation Reaction显示文摘A ferrocenylsulfides 1-2 mediated sulfur ylide epoxidation reaction was found to stereoselectively afford trans-oxiranes in excellent yields with recoverable starting material. | LeiWANG ZhiZhenHUANG | 2005 | Chinese Chemical Letters2005，16，7： | 0 |
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