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| 1 | Mapping epistasis and environment × QTX interaction based on four-omics genotypes for the detected QTX loci controlling complex traits in tobacco显示文摘Using newly developed methods and software, association mapping was conducted for chromium content and total sugar in tobacco leaf, based on four-omics datasets. Our objective was to collect data on genotype and phenotype for 60 leaf samples at four developmental stages, from three plant architectural positions and for three cultivars that were grown in two locations. Association mapping was conducted to detect genetic variants at quantitative trait SNP(QTS) loci, quantitative trait transcript(QTT) differences,quantitative trait protein(QTP) variability, and quantitative trait metabolite(QTM) changes,which can be summarized as QTX locus variation. The total heritabilities of the four-omics loci for both traits tested were 23.60% for epistasis and 15.26% for treatment interaction.Epistasis and environment × treatment interaction had important impacts on complex traits at all-omics levels. For decreasing chromium content and increasing total sugar in tobacco leaf, six methylated loci can be directly used for marker-assisted selection, and expression of ten QTTs, seven QTPs and six QTMs can be modified by selection or cultivation. | Liyuan Zhou Ruiyuan Li Longjiang Fan Yuewei Shi Zhihong Wang Shengdong Xie Yijie Gui Xueliang Ren Jun Zhu | 2013 | The Crop Journal2013，1，2： | 4 |
| 2 | Development of DArT markers for a linkage map of flue-cured tobacco显示文摘Tobacco(Nicotiana tabacum) is one of the most economically important nonfood crops,and flue-cured tobacco accounts for approximately 80% of world tobacco production.An extremely narrow genetic diversity in the tobacco pool has led to a low efficiency of PCR-based molecular markers(such as AFLP and SSR).Diversity Arrays Technology(DArT) is a high-throughput hybridisation-based marker system that has been developed in many plants including wheat,which,like tobacco,has a complex genome.In this study,we developed a tobacco DArT chip that included 7680 representative sequence tags based on typical tobacco accessions.The 1076 DArT markers of flue-cured tobacco were identified and most(82.1%) of their polymorphism information contents(PICs) were greater than 0.4.An integrated linkage map that included 851 markers(238 DArT and 613 SSR),which is the highest density map of flue-cured tobacco to date,was constructed.This chip-based DArT system provides an alternative in high-throughput marker genotyping for tobacco. | LU XiuPing GUI YiJie XIAO BingGuang LI YongPing TONG ZhiJun LIU Yun BAI XueFei WU WeiRen XIA Ling HUTTNER Eric KILIAN Adrzej FAN LongJiang | 2013 | Chinese Science Bulletin2013，58，6： | 4 |
| 3 | Elimination of a Retrotransposon for Quenching Genome Instability in Modern Rice显示文摘Transposable elements(TEs)constitute the most abundant portions of plant genomes and can dramatically shape host genomes during plant evolution.They also play important roles in crop domestication.However,whether TEs themselves are also selected during crop domestication has remained unknown.Here,we identify an active long terminal repeat(LTR)retrotransposon,HUO,as a potential target of selection during rice domestication and breeding.HUO is a low-copy-number LTR retrotransposon,and is active under natural growth conditions and transmitted through male gametogenesis,preferentially inserting into genomic regions capable of transcription.HUO exists in all wild rice accessions and about half of the archaeological rice grains(1200–7000 years ago)and landraces surveyed,but is absent in almost all modern varieties,indicating its gradual elimination during rice domestication and breeding.Further analyses showed that HUO is subjected to strict gene silencing through the RNA-directed DNA methylation pathway.Our results also suggest that multiple HUO copies may trigger genomic instability through altering genome-wide DNA methylation and small RNA biogenesis and changing global gene expression,resulting in decreased disease resistance and yield,coinciding with its elimination during rice breeding.Together,our study suggests that negative selection of an active retrotransposon might be important for genome stability during crop domestication and breeding. | Yu Peng Yingying Zhang Yijie Gui Dong An Junzhong Liu Xun Xu Qun Li Junmin Wang Wen Wang Chunhai Shi Longjiang Fan Baorong Lu Yiwen Deng Sheng Teng Zuhua He | 2019 | Molecular Plant2019，12，10： | 2 |
| 4 | Ancient DNA sequences of rice from the low Yangtze reveal significant genotypic divergence显示文摘Rice (Oryza sativa) was first domesticated in the lower and middle Yangtze regions of China, and rice remains have been found in many Chinese archaeological sites. Until now, only phenotypic archeobotanical evidence, such as the spikelet bases of ancient grains, has been used to speculate on the domestication process and domestication rate of rice. In this study, we sequenced 4 genomic segments from rice remains in Tianluoshan, a site of the local Hemudu Neolithic culture in the low Yangtze and two other archaeological sites (~2400 and 1200 BC, respectively). We compared our sequences with those of the current domesticated and wild rice (O. rufipogon) populations. At least two genotypes were found in the remains from each site, suggesting a heterozygotic state of the rice seeds. One ancient genotype was not found in the current domesticated population and might have been lost. The rice remains belonged to the japonica group, and most if not all were japonica-type, suggesting that the remains might be at an early stage of indica-japonica divergence or an indica-japonica mixture. We also identified sequences with significant similarity to those from species of Sapindales, Zygophyllales, and Brassicales, which is consistent with the identification of other plant remains in the Tianluoshan site and the common rice field weeds such as mustards in southern China. | FAN LongJiang GUI YiJie ZHENG YunFei WANG Yu CAI DaGuang YOU XiuLing | 2011 | Chinese Science Bulletin2011，56，28： | 2 |
| 5 | Identification of NBS-Type Resistance Gene Homologs in Tobacco Genome显示文摘 | Xiaodong Leng Bingguang Xiao Sheng Wang Yijie Gui Yu Wang Xiuping Lu Jiahua Xie Yongping Li Longjiang Fan | 2010 | Plant Molecular Biology Reporter2010，，1： | 1 |
| 6 | Application of different molecular sieves in photothermal catalysis of Jatropha oil显示文摘The preparation of green and economical bio-aviation fuel is a priority for the sustainable development industry.In this study,Jatropha oil was used as a raw material to catalyze the conversion of raw material to aviation kerosene fraction by photothermal coupling under the conditions of light and low temperature.The correlations among conversion rate,target alkane selectivity,composition distribution,and catalyst microstructure were investigated by X-ray diffraction(XRD),high-resolution transmission electron microscopy(HRTEM),nitrogen(N2)adsorption and desorption,X-ray fluorescence(XRF),ammoniatemperature programmed desorption(NH3-TPD),ultraviolet-visible spectrophotometry(UV-Vis),and other characterization.The correlation between conversion and target alkane selectivity and composition distribution and catalyst microstructure was investigated,and different modification methods and different molecular sieve materials were selected.The results showed that the molecular sieves modified with the solid dispersion method could retain the structural stability of titanium dioxide(TiO2)and molecular sieves to a great extent while slightly enhancing the pore capacity and pore size of the catalyst to make it easier to adsorb reactants;the introduction of active metal platinum(Pt)could reduce the forbidden bandwidth of the catalyst,increase the weak acid amount of the catalyst,improve the adsorption capacity of hydrogen(H2),and thus improve the catalytic ability,resulting in a suitable catalyst for this study:P-21.The photothermal catalytic reaction of Jatropha oil using P-21 catalyst obtained 97.21%conversion and 74.99%selectivity of the target alkanes under the optimal process parameters.The results of this study provide effective catalyst parameters for research in the field of clean energy. | Yijie Yang Longlong Ma Lumin Sun Xinghua Zhang Chao Gui Yubao Chen | 2023 | International Journal of Agricultural and Biological Engineering2023，16，5： | 0 |
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