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| 1 | Comparison of Chlorophyll and Photosynthesis Parameters of Floating and Attached Ulva prolifera显示文摘In mid-May 2008 a serious green tide caused mainly by floating Ulva prolifera(Mller) J.Agardh(Chlorophyta,Ulvales) thalli struck the coastal area of Qingdao,China.To understand the present physiological conditions of the floating alga,in this work both laboratory and field investigations were conducted on the floating U.prolifera thalli in comparison with the attached U.prolifera thalli collected from the area.The floating thalli of three distinctively different colors and attached thalli at three different stages of sporangium formation process were characterized under a microscope,while their photosynthetic parameters were determined with chlorophyll fluorescence technology.On the other hand,the sporangium formation status of the floating U.prolifera thalli was surveyed both in the laboratory and in the field.Comparisons showed that both of the paired morphological characteristics and the paired physiological parameters of the floating and attached U.prolifera thalli were consistent.Furthermore,some spores were confirmed in the field and some motile particles were found within the floating thalli.These results suggest that the floating U.prolifera thalli with different colors could be at different stages of sporangium formation.However,our results also showed that the floating alga thalli have only a limited reproductive potential.This might limit the duration and the further geographic expansion of the green algal bloom. | Apeng Lin Songdong Shen Guangce Wang Qianqian Yi Hongjin Qiao Jianfeng Niu Guanghua Pan | 2011 | Journal of Integrative Plant Biology2011，53，1： | 20 |
| 2 | Study on the photosynthetic performances of Enteromorpha prolifera collected from the surface and bottom of the sea of Qingdao sea area显示文摘In this work, the photosynthetic performances of Enteromorpha prolifera thalli collected from the sur- face and bottom of the sea of Qingdao sea area were studied with chlorophyll fluorescence and oxy- graph technology. The samples with the highest photosynthetic activity among their kinds, the floating thalli from the sea surface of the south of the Qingdao Olympic Sailing Center and the sedimentary thalli from the mud surface of the bottom Tuandao bay, were chosen as representatives of surface thalli and bottom thalli, respectively. The results showed that the maximal PSII quantum yield of the floating thalli was significantly lower than the normal level although their photosynthetic activities were relatively high; the photosynthetic potential of the thalli form the mud surface was extremely low. Thus, it is indicated that the floating thalli are seriously stressed by their environment and the thalli from the mud surface are already dead or are dying. On the other hand, the results of the laboratory cultivation showed that the sedimentary thalli cannot regain normal photosynthetic activity even under normal illumination condi- tions. Thus, the thalli from the mud surface cannot become reproductive source of the alga even if they can reach sea surface again. Therefore, a preliminary conclusion can be reached that, up to mid-July 2008, the environmental conditions of the Qingdao sea area are not suitable for the growth of the alga E. prolifera and for this reason the biomass of E. prolifera, in the area, could be declining. | LIN APeng WANG Chao QIAO HongJin PAN GuangHua WANG GuangCe SONG LiYun WANG ZhiYuan SUN Song ZHOU BaiCheng | 2009 | Chinese Science Bulletin2009，54，3： | 14 |
| 3 | Enrichment and hydrogen production by marine anaerobic hydrogen-producing microflora显示文摘Acid, alkali, heat-shock, KNO3 and control pretreatment methods applied to anaerobic sludge were evaluated for their ability to selectively enrich the marine hydrogen-producing mixed microflora. Sea-water culture medium was used as the substrate. The hydrogen yield of pretreated microflora was higher than that of the un-pretreated control (P < 0.05). Among the pretreatment methods studied, heat-shock pretreatment yielded the greatest hydrogen production, which was 14.6 times that of the control. When the effect of initial pH on hydrogen production of heat-shock pretreated samples was studied, hydrogen was produced over the entire pH range (pH 4 - 10). The hydrogen yield peaked at initial pH 8 (79 mL/g sucrose) and then steadily decreased as the initial pH increased. Sucrose con-sumption was high at neutral initial pH. During the process of hydrogen production, pH decreased gradually, which indicated that the acquired microflora consisted of acidogenic bacteria. | CAI JinLing WANG GuangCe LI YanChuan ZHU DaLing PAN GuangHua | 2009 | Chinese Science Bulletin2009，54，15： | 7 |
| 4 | Characterization of small RNAs from Ulva prolifera by high-throughput sequencing and bioinformatics analysis显示文摘The seaweed Ulva prolifera exists in 2 different states; attached to rocks or free-floating. However, there is little difference between the structures of the 2 states. U. prolifera thalli show significant differences in growth rate, with the attached thalli growing at a normal rate and free-floating thalli growing at a much faster rate. This raised the possibility that the growth of the two states may be regulated differently. miRNAs are important post-transcriptional regulators. In higher plants and animals, miRNAs have been extensively studied but they have been rarely studied in algae. To identify U. prolifera miRNAs and to investigate their possible roles in proliferation, we constructed and sequenced small RNA (sRNA) libraries from U. prolifera. Our results show that U. prolifera has a complex small RNA system that might play important roles in various processes. | HUANG AiYou WANG GuangCe HE LinWen NIU JianFeng ZHANG BaoYu | 2011 | Chinese Science Bulletin2011，56，27： | 3 |
| 5 | Transcriptome analysis of carotenoid biosynthesis in Dunaliella salina under red and blue light显示文摘The quality of light is an important abiotic factor that affects the growth and development of photosynthetic organisms.In this study,we exposed the unicellular green alga Dunaliella salina to red(660 nm)and blue(450 nm)light and analyzed the cell growth,total carotenoid content,and transcriptomes.The growth of D.salina was enhanced by illumination with red light,whereas blue light was not able to promote the algal growth.In contrast,the total carotenoid content increased under both red and blue light.The RNA of D.salina was sequenced and the transcriptomic response of algal cells to red and blue light was investigated.Six transcripts encoding for the blue light receptor cryptochrome were identified,and transcripts involved in the carotenoid metabolism were up-regulated under both red and blue light.Transcripts encoding for photoprotective enzymes related to the scavenging of reactive oxygen species were up-regulated under blue light.The present transcriptomic study provides a more comprehensive understanding of carotenoid biosynthesis in D.salina under different wavelengths of light. | LI Yuanxiang CAI Xuehua GU Wenhui WANG Guangce | 2020 | Journal of Oceanology and Limnology2020，38，1： | 3 |
| 6 | Identification and bioinformatics analysis of pseudogenes from whole genome sequence of Phaeodactylum tricornutum显示文摘Pseudogenes share sequence similarities with functional genes,but in general they have lost their protein-coding ability.The identification of pseudogenes is a very important step in genome annotation.Phaeodactylum tricornutum is a marine diatom that is rich in polyunsaturated fatty acids(PUFAs).The genome of P.tricornutum has been completely sequenced.To identify pseudogenes in P.tricornutum,we developed a pipeline to discover and characterize pseudogenes.We identified a total of 1654 'true' processed pseudogenes,714 duplicated pseudogenes and 4729 pseudogene fragments.The results of the bioinformatics analysis indicated that the genome sequence of P.tricornutum contained many pseudogenes and pseudogene fragments. | JI ChangMian HUANG AiYou LIU WenLing PAN GuangHua WANG GuangCe | 2013 | Chinese Science Bulletin2013，58，9： | 2 |
| 7 | Cloning and sequence analysis of the partial sequence of the rbcL from Bryopsis hypnoides显示文摘The partial sequence of the rbcL from Bryopsis hypnoides, including the sequences of the upstream, extron and partial intron, was amplified by PCR and their sequences were determined. With Spinacia oleracea as the outgroup, neighbor-joining method and maximum parsimony method were used respectively to build phylogenetic trees according to the rbcL exon sequence among 13 species that were the typical species of six phyla. Two kinds of trees showed clearly that there were two groups among those species, the green lineage and the non-green lineage. And the relationships of algae in the green lineage were similar in the two trees but those in the non-green lineage were not consistent. Analysis of codon preference indicated that the codon preference of the rbcL exon of Bryopsis hypnoides distinctly differed from that of the relevant sequence of photosynthetic bacteria. | TIAN Chao WANG Guangce YE Naihao ZHANG Baoyu FAN Xiaolei ZENG Chengkui | 2005 | Acta Oceanologica Sinica2005，24，5： | 2 |
| 8 | Characteristics for production of hydrogen and bioflocculant by Bacillus sp. XF-56 from marine intertidal sludge显示文摘 | Hongyan Liu Guochao Chen Guangce Wang | 2015 | International Journal of Hydrogen Energy2015，，3： | 1 |
| 9 | Formation and ear- ly development of tetraspores of Gracilaria lemaneiformis ( Gracilaria, Gracilarlaccae ) under laboratory conditions 显示文摘 | Naihao Ye Hongxia Wang Guangce Wang | 2006 | Aquaculture2006，254，14： | 1 |
| 10 | Comparison be- tween Compsopogon coeruleus and Porphyra yezoensis in their 02 evo- lution rates and phycobilisome composition显示文摘 | LI Yingxia WANG Guangce NIU Jianfeng | 2008 | Russian Journal of Plant Physiology2008，55，2： | 1 |
| 11 | A comparative study of thermolysis characteristics and kinetics of seaweeds and fir wood显示文摘 | JUN WANG GUANGCE WANG MINGXU ZHANG | 2006 | Process Biochemistry2006，41，8： | 1 |
| 12 | Study on early-stage development of conchospore in Porphyra yezoensis Ueda显示文摘 | FAN XIAOLEI WANG GUANGCE LI DEMAO | 2008 | Aquaculture2008，，278： | 1 |
| 13 | Large-scale recovery of c-phycocyanin from Spirulina plat- ens/s using expanded bed adsorption chromatography 显示文摘 | NIU Jianfeng WANG Guangce LIN Xiangzhi | 2007 | Journal of Chromatography B2007，850，12： | 1 |
| 14 | Development of taxonomic rRNA-targeted probes of two harmful algae: Prorocentrum minimum and Karenia mikimotoi by fluorescence in situ hybridization显示文摘Harmful algal blooms recently have been under the spotlight throughout the world, because of their negative impact on the marine environment, aquaculture, fisheries as well as public health. The development of methods for rapid and precise identification and quantification of causative species is essential for the warning and monitoring of blooms, among which the techniques based on taxonomic probes are the most favored. In this study, two harmful algae, i.e., Prorocentrum minimum and Karenia mikimotoi were taken into consideration. The partial large subunit rDNA (D1-D2) of both species were firstly PCR-amplified, cloned and sequenced. The obtained sequences were then introduced to carry out alignment analysis for gene specific regions. Three respective candidate probes for each species were designed and used to screen the optimal probe by performing fluorescence in situ hybridization (FISH) tests. The results showed that the probes Pmin0443 and Kmik0602 displayed the best hybridization for P. minimum and K. mikimotoi, respectively. Both the specific (taxonomic) (Pmin0443 and Kmik0602) and the control probes (UniC0512 and UniR0499) were used for cross-reactivity tests with other microalgae in our laboratory. The probes Pmin0443 and Kmik0602 are specific and could be served as taxonomic probes introduced into the techniques targeting rRNA, such as FISH, sandwich hybridization, and DNA-microarray assay of P. minimum and K. mikimotoi in the future. Finally, FISH analyses with both probes were performed on the simulated field samples. The probes could hybridize exclusively with the target cells well, and no significant difference (p >0.05) was observed in the cell densities of the samples determined by FISH and light microscopy (LM). All suggest that the probes are specific and could be introduced into FISH for the monitoring of both harmful algae. | CHEN Guofu WANG Quanfu ZHANG Chunyun ZHANG Baoyu WANG Guangce LU Douding XU Zhong YAN Peishen | 2013 | Acta Oceanologica Sinica2013，32，2： | 1 |
| 15 | A comparative study of thermolysis characteristics and kinetics of seaweeds and fir wood显示文摘 | Wang Jun Wang Guangce Zhang Mingxu | 2006 | Process Biochemistry2006，41，： | 1 |
| 16 | Comparative Analysis of MicroRNAs between Sporophyte and Gametophyte of Porphyra yezoensis显示文摘 | Linwen He Aiyou Huang Songdong Shen Jianfeng Niu Guangce Wang Paul Denny | 2012 | <journal-title>Comparative and Functional Genomics2012，，： | 1 |
| 17 | Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography显示文摘 | NIU Jianfeng WANG Guangce LIN Xiangzhi | 2007 | Journal of Chromatography B2007，850，12： | 1 |
| 18 | A comparative study of thermolysis characteristics of sea weed and fir wood显示文摘 | Wang Jun Wang Guangce Zhang Mingxu | 2006 | Process Biochemistry2006，41，： | 1 |
| 19 | Effect of herba centellae on the expression of HGF and MCP-1显示文摘 | Zhu Zhang Guangce Wang Jiwei Ma Haofei Liu Xitao Zhang Guangling Zhu | 2013 | Experimental and Therapeutic Medicine2013，，2： | 1 |
| 20 | A comparative study of thermolysis characteristics and kinet- ics of seaweeds and fir wood 显示文摘 | Wang Jun Wang Guangce Zhang Mingxu | 2006 | Process Biochemistry2006，41，8： | 1 |
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