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| 1 | Influence of Gd_2O_3 and Yb_2O_3 Co-doping on Phase Stability,Thermo-physical Properties and Sintering of 8YSZ显示文摘The role of multicomponent rare earth oxides in phase stability, thermophysical properties and sintering for ZrO2-based thermal barrier coatings (TBCs) materials is investigated. 8YSZ codoped with 3 mol% Gd2O3 and 3 mol% Yb2O3 (GYb-YSZ) powders are synthesized by solid state reaction for 24 h at various temperatures. As temperature increases, stabilizers are dissolved into zirconia matrix gradually. Synthesized at 1 500 °C, GYb-YSZ is basically composed of cubic phase. GYb-YSZ exhibits excellent phase stability and sinters lower than 8YSZ by nearly three times. The thermal conductivity of GYb-YSZ is much lower than that of 8YSZ, and the thermal expansion coefficient of GYb-YSZ is comparable to that of 8YSZ. The influence of Gd2O3 and Yb2O3 co-doping on phase stability, thermal conductivity and sintering of 8YSZ is discussed. | ZHANG Yanli GUO Lei YANG Yongping GUO Hongbo ZHANG Hongju GONG Shengkai | 2012 | Chinese Journal of Aeronautics2012，25，6： | 17 |
| 2 | The concise fractional Fourier transform and its application in detection and parameter estimation of the linear frequency-modulated signal显示文摘A concise fractional Fourier transform(CFRFT) is proposed to detect the linear frequency-modulated(LFM) signal with low signal to noise ratio(SNR).The frequency axis in time-frequency plane of the CFRFT is rotated to get the spectrum of the signal in different angles using chirp multiplication and Fourier transform(FT).For LFM signal which distributes as a straight line in time-frequency plane,the CFRFT can gather the energy in the corresponding angle as a peak and improve the detection SNR,thus the LFM signal of low SNR can be detected.Meanwhile,the location of the peak value relates to the parameters of the LFM signal.Numerical simulations and experimental results show that,the proposed method can be used to efficiently detect the LFM signal masked by noise and to estimate the signal's parameters accurately.Compared with the conventional fractional Fourier transform(FRFT),the CFRFT reduces the transform complexity and improves the real-time detection performance of LFM signal. | CHEN Yanli GUO Lianghao GONG Zaixiao | 2017 | Chinese Journal of Acoustics2017，36，1： | 13 |
| 3 | A self-organizing multimodal multi-objective pigeon-inspired optimization algorithm显示文摘Multi-objective optimization algorithms have recently attracted much attention as they can solve problems involving two or more conflicting objectives effectively and efficiently. However, most existing studies focus on improving the performance of the solutions in the objective spaces. This paper proposes a novel multimodal multi-objective pigeon-inspired optimization(MMOPIO) algorithm where some mechanisms are designed for the distribution of the solutions in the decision spaces. First, MMOPIO employs an improved pigeon-inspired optimization(PIO) based on consolidation parameters for simplifying the structure of the standard PIO. Second, the self-organizing map(SOM) is combined with the improved PIO for better control of the decision spaces, and thus, contributes to building a good neighborhood relation for the improved PIO.Finally, the elite learning strategy and the special crowding distance calculation mechanisms are used to prevent premature convergence and obtain solutions with uniform distribution, respectively. We evaluate the performance of the proposed MMOPIO in comparison to five state-of-the-art multi-objective optimization algorithms on some test instances, and demonstrate the superiority of MMOPIO in solving multimodal multi-objective optimization problems. | Yi HU Jie WANG Jing LIANG Kunjie YU Hui SONG Qianqian GUO Caitong YUE Yanli WANG | 2019 | Science China(Information Sciences)2019，62，7： | 10 |
| 4 | A Brief Introduction to BNU-HESM1.0 and Its Earth Surface Temperature Simulations显示文摘Integrated assessment models and coupled earth system models both have their limitations in understanding the interactions between human activity and the physical earth system. In this paper,a new human–earth system model,BNUHESM1.0,constructed by combining the economic and climate damage components of the Dynamic Integrated Model of Climate Change and Economy to the BNU-ESM model,is introduced. The ability of BNU-HESM1.0 in simulating the global CO2 concentration and surface temperature is also evaluated. We find that,compared to observation,BNU-HESM1.0underestimates the global CO2 concentration and its rising trend during 1965–2005,due to the uncertainty in the economic components. However,the surface temperature simulated by BNU-HESM1.0 is much closer to observation,resulting from the overestimates of surface temperature by the original BNU-ESM model. The uncertainty of BNU-ESM falls within the range of present earth system uncertainty,so it is the economic and climate damage component of BNU-HESM1.0 that needs to be improved through further study. However,the main purpose of this paper is to introduce a new approach to investigate the complex relationship between human activity and the earth system. It is hoped that it will inspire further ideas that prove valuable in guiding human activities appropriate for a sustainable future climate. | YANG Shili DONG Wenjie CHOU Jieming FENG Jinming YAN Xiaodong WEI Zhigang YUAN Wenping GUO Yan TANG Yanli HU Jiacong | 2015 | Advances in Atmospheric Sciences2015，32，12： | 8 |
| 5 | Downregulation of LINC01296 suppresses non-small-cell lung cancer via targeting miR-143-3p/ATG2B显示文摘The 5-year survival rate of lung cancer is one of the lowest among various malignant tumors.Long noncoding RNAs(lncRNAs),noncoding RNAs longer than 200 nucleotides,can function either as tumor suppressors or as oncogenes.The aim of this study is to investigate the function of lncRNA LINC01296 and its molecular mechanism in non-small-cell lung cancer(NSCLC).According to the Gene Expression Omnibus database,10 differentially expressed lncRNAs in NSCLC cells and patient tissues are upregulated.LINC01296 is the one with the most significant overexpression.Knockdown of LINC01296 inhibits the growth and migration,arrests the cell cycle,and promotes the apoptosis of NSCLC cells.Knocking down LINC01296 in vivo suppresses tumor growth and metastasis.LINC01296 also acts as the sponge of miR-143-3p.Lowering the expression of LINC01296 leads to decreased expression of autophagy-related 2B,ATG2B,a target gene of miR-143-3p.Moreover,downregulation of LINC01296 promotes paclitaxel sensitivity in NSCLC.These results demonstrated that the LINC01296/miR-143-3p/ATG2B axis is crucial in promoting the development of NSCLC and paclitaxel resistance.Our study may provide new ideas for the further research of clinical chemotherapy of NSCLC in the near future. | Yanli Li Hui Zhang Jing Guo Wanqiu Li Xianyi Wang Caiyan Zhang Qiangling Sun Zhongliang Ma | 2021 | Acta Biochimica et Biophysica Sinica2021，53，12： | 8 |
| 6 | Intestinal toxicity of deoxynivalenol is limited by supplementation with Lactobacillus plantarum JM113 and consequentially altered gut microbiota in broiler chickens显示文摘Background: Limited research has focused on the effect of Lactobacillus on the intestinal toxicity of deoxynivalenol(DON).The present study was conducted to investigate the role of Lactobacillus plantarum(L.plantarum) JM113 in protecting against the intestinal toxicity caused by DON.Methods: A total of 144 one-day-old healthy Arbor Acres broilers were randomly distributed into 3 treatments,including the CON(basal diet),the DON(extra 10 mg/kg deoxynivalenol),and the DL(extra 1 × 109 CFU/kg L.plantarum JM113 based on DON group) treatments.The growth performance,organ indexes,intestinal morphology,pancreatic digestive enzymes,intestinal secreted immunoglobulin A(sIgA),jejunal transcriptome,and intestinal microbiota were evaluated.Results: Compared with the CON and DL groups,the DON supplementation altered intestinal morphology,especially in duodenum and jejunum,where villi were shorter and crypts were deeper(P < 0.05).Meanwhile,the significantly decreased mRNA expression of jejunal claudin-1 and occludin(P < 0.05),ileal rBAT and jejunal GLUT1 of 21-day-old broilers(P < 0.05),as well as duodenal PepT1 and ileal rBAT of 42-day-old broilers were identified in the DON group.Moreover,supplementation with L.plantarum JM113 could increase duodenal expression of IL-10 and IL-12 of 21-dayold broilers,ileal s IgA of 42-day-old broilers,and the bursa of Fabricius index of 21-day-old broilers.Further jejunal transcriptome proved that the genes related to the intestinal absorption and metabolism were significantly reduced in the DON group but a significant increase when supplemented with extra L.plantarum JM113.Furthermore,the bacteria related to nutrient utilization,including the Proteobacteria,Escherichia,Cc-115(P < 0.05),Lactobacillus and Prevotella(P < 0.1) were all decreased in the DON group.By contrast,supplementation with L.plantarum JM113 increased the relative abundance of beneficial bacterium,including the Bacteroidetes,Roseburia,Anaerofustis,Anaerostipe,and Ruminococcus bromi(P < 0.05).Specifically,the increased abundance of bacteria in the DL group could be proved by the significantly increased caecal content of propionic acid,n-Butyric acid,and total short-chain fatty acid.Conclusions: L.plantarum JM113 enhanced the digestion,absorption,and metabolic functions of the gut when challenged with DON by reducing the injury to intestinal barriers and by increasing the abundance of beneficial bacterium. | Shengru Wu Yanli Liu Yongle Duan Fangyuan Wang Fangshen Guo Fang Yan Xiaojun Yang Xin Yang | 2019 | Journal of Animal Science and Biotechnology2019，10，1： | 7 |
| 7 | Rush-hour aromatic and chlorinated hydrocarbons in selected subway stations of Shanghai,China显示文摘Air samples were collected simultaneously at platform,mezzanine and outdoor in five typical stations of subway system in Shanghai,China using stainless steel canisters and analyzed by gas chromatography-mass selective detector (GC-MSD) after cryogenic pre-concentration.Benzene,toluene,ethylbenzene and xylenes (BTEX) at the platforms and mezzanines inside the stations averaged (10.3±2.1),(38.7±9.0),(19.4±10.1) and (30.0±11.1) μg/m 3,respectively;while trichloroethylene (TrCE),tetrachloroethylene (TeCE) and para-dichlorobenzene (pDCB),vinyl chloride and carbon tetrachloride were the most abundant chlorinated hydrocarbons inside the stations with average levels of (3.6±1.3),(1.3±0.5),(4.1±1.1),(2.2±1.1) and (1.2±0.3) μg/m 3,respectively.Mean levels of major aromatic and chlorinated hydrocarbons were higher indoor (platforms and mezzanines) than outdoor with average indoor/outdoor (I/O) ratios of 1.1-9.5,whereas no significant indoor/outdoor differences were found except for benzene and TrCE.The highly significant mutual correlations (p<0.01) for BTEX between indoor and outdoor and their significant correlation (p<0.05) with methyl tert-butyl ether (MTBE),a marker of traffic-related emission without other indoor and outdoor sources,indicated that BTEX were introduced into the subway stations from indoor/outdoor air exchange and traffic emission should be their dominant source.TrCE and pDCB were mainly from indoor emission and TeCE might have both indoor emission sources and contribution from outdoor air,especially in the mezzanines. | Yanli Zhang Chunlei Li Xinming Wang Hai Guo Yanli Feng Jianmin Chen | 2012 | Journal of Environmental Sciences2012，24，1： | 6 |
| 8 | A prediction method of natural gas hydrate formation in deepwater gas well and its application显示文摘To prevent the deposition of natural gas hydrate in deepwater gas well,the hydrate formation area in wellbore must be predicted.Herein,by comparing four prediction methods of temperature in pipe with field data and comparing five prediction methods of hydrate formation with experiment data,a method based on OLGA&PVTsim for predicting the hydrate formation area in wellbore was proposed.Meanwhile,The hydrate formation under the conditions of steady production,throttling and shut-in was predicted by using this method based on a well data in the South China Sea.The results indicate that the hydrate formation area decreases with the increase of gas production,inhibitor concentrations and the thickness of insulation materials and increases with the increase of thermal conductivity of insulation materials and shutdown time.Throttling effect causes a plunge in temperature and pressure in wellbore,thus leading to an increase of hydrate formation area. | Yanli Guo Baojiang Sun Keke Zhao Hongkun Zhang | 2016 | Petroleum2016，2，3： | 5 |
| 9 | Genetic testing and PGD for unexplained recurrent fetal malformations with MAGEL2 gene mutation显示文摘Birth defects are caused by multiple factors,such as chromosome abnormality,environmental factors,and maternal factors.In this study,we focused on exploring the genetic causes of a non-consanguineous couple who suffered from four times of unsuccessful pregnancy due to unexplained recurrent fetal malformations with similar symptoms and normal chromosome copy number variations.Using trio-whole exome sequencing(trio-WES) for this couple and one of the affected fetuses,we found a mutation,c.1996 delC on the maternal imprinted gene MAGEL2 that was carried by the affected fetus and husband,leading to Schaaf-Yang syndrome.To screen this mutation,we further performed preimplantation genetic diagnosis(PGD) strategy followed by a gene pedigree validation and pathogenicity analysis.After the transfer of a PGD-screened embryo,a normal newborn without previous abnormal symptoms was born(February 15,2019).We present the first data that identified a pathogenic gene(MAGEL2 c.1996 delC) in a fetus with Schaaf-Yang syndrome in the EAS(East Asian) database and overcame this genetic defect by using processed PGD for this couple based on the WES results. | Wei Guo Yanli Nie Zhiqiang Yan Xiaohui Zhu Yuqian Wang Shuo Guan Ying Kuo Wenxin Zhang Xu Zhi Yuan Wei Liying Yan Jie Qiao | 2019 | Science China(Life Sciences)2019，62，7： | 4 |
| 10 | Link Prediction in Brain Networks Based on a Hierarchical Random Graph Model显示文摘Link prediction attempts to estimate the likelihood of the existence of links between nodes based on available brain network information, such as node attributes and observed links. In response to the problem of the poor efficiency of general link prediction methods applied to brain networks, this paper proposes a hierarchical random graph model based on maximum likelihood estimation. This algorithm uses brain network data to create a hierarchical random graph model. Then, it samples the space of all possible dendrograms using a Markov-chain Monte Carlo algorithm. Finally, it calculates the average connection probability. It also employs an evaluation index.Comparing link prediction in a brain network with link prediction in three different networks(Treponemapallidum metabolic network, terrorist networks, and grassland species food webs) using the hierarchical random graph model, experimental results show that the algorithm applied to the brain network has the highest prediction accuracy in terms of AUC scores. With the increase of network scale, AUC scores of the brain network reach 0.8 before gradually leveling off. In addition, the results show AUC scores of various algorithms computed in networks of eight different scales in 28 normal people. They show that the HRG algorithm is far better than random prediction and the ACT global index, and slightly inferior to local indexes CN and LP. Although the HRG algorithm does not produce the best results, its forecast effect is obvious, and shows good time complexity. | Yanli Yang Hao Guo Tian Tian Haifang Li | 2015 | Tsinghua Science and Technology2015，20，3： | 4 |
| 11 | Magnetic properties of Nd_2Fe_(14)B/α-Fe nanocomposite magnets with yttrium addition显示文摘The phase evolution and magnetic properties of Nd9?xYxFe72Ti2Zr2B15 (x = 0,0.5,1,and 2) melt-spun nanocomposite ribbons were studied.It is found that Y addition not only enhances the formability of amorphous phase in the alloy,but also stabilizes the amorphous phase during the annealing treatment.The appropriate content of Y addition effectively enhances the remanence (Jr) of the annealed sample.The residual amorphous intergranular phase in the annealed sample optimizes the squareness of the loop,resulting in an larger maximum energy product (BH)max.The best magnetic properties,Jr = 0.78 T,Hci (coercivity) = 923.4 kA/m,and (BH)max = 98.5 kJ/m3,were obtained from the Nd8YFe72Ti2Zr2B15 ribbon spun at Vs = 4 m/s and annealed at 700°C for 10 min,which is composed of Nd2Fe14B,α-Fe,and amorphous phase. | CHEN Zhi'an SUI Yanli GUO Zhimeng | 2010 | Rare Metals2010，29，3： | 3 |
| 12 | Palm oil protectsα-linolenic acid from rumen biohydrogenation and muscle oxidation in cashmere goat kids显示文摘Background:In ruminants,dietary C18:3n-3 can be lost through biohydrogenation in the rumen;and C18:3n-3 that by-passes the rumen still can be lost through oxidation in muscle,theoretically reducing the deposition of C18:3n-3,the substrate for synthesis of poly-unsaturated fatty acids(n-3 LCPUFA)in muscle.In vitro studies have shown that rumen hydrogenation of C18:3n-3 is reduced by supplementation with palm oil(rich in cis-9 C18:1).In addition,in hepatocytes,studies with neonatal rats have shown that cis-9 C18:1 inhibits the oxidation of C18:3n-3.It therefore seems likely that palm oil could reduce both rumen biohydrogenation of C18:3n-3 and muscle oxidation of C18:3n-3.The present experiment tested whether the addition of palm oil to a linseed oil supplement for goat kids would prevent the losses of C18:3n-3 and thus improve the FA composition in two muscles,Longissimus dorsi and Biceps femoris.To investigate the processes involved,we studied the rumen bacterial communities and measured the mRNA expression of genes related to lipid metabolism in Longissimus dorsi.Sixty 4-month-old castrated male Albas white cashmere kids were randomly allocated among three dietary treatments.All three diets contained the same ingredients in the same proportions,but differed in their fat additives:palm oil(PMO),linseed oil(LSO)or mixed oil(MIX;2 parts linseed oil plus 1 part palm oil on a weight basis).Results:Compared with the LSO diet,the MIX diet decreased the relative abuandance of Pseudobutyrivibrio,a bacterial species that is positively related to the proportional loss rate of dietary C18:3n-3 and that has been reported to generate the ATP required for biohydrogenation(reflecting a decrease in the abundance of rumen bacteria that hydrogenate C18:3n-3 in MIX kids).In muscle,the MIX diet increased concentrations of C18:3n-3,C20:5n-3,C22:6n-3,and n-3 LCPUFA,and thus decreased the n-6/n-3 ratio;decreased the mRNA expression of CPT1β(a gene associated with fatty acid oxidation)and increased the mRNA expression of FADS1 and FADS2(genes associated with n-3 LCPUFA synthesis),compared with the LSO diet.Interestingly,compared to Longissimus dorsi,Biceps femoris had greater concentrations of PUFA,greater ratios of unsaturated fatty acids/saturated fatty acids(U/S),and poly-unsaturated fatty acids/saturated fatty acids(P/S),but a lesser concentration of saturated fatty acids(SFA).Conclusions:In cashmere goat kids,a combination of linseed and palm oils in the diet increases the muscle concentration of n-3 LCPUFA,apparently by decreasing the relative abundance of rumen bacteria that are positively related to the proportional loss rate of dietary C18:3n-3,by inhibiting mRNA expression of genes related to C18:3n-3 oxidation in muscle,and by up-regulating mRNA expression of genes related to n-3 LCPUFA synthesis in muscle,especially in Longissimus dorsi. | Xue Wang Graeme B.Martin Qi Wen Shulin Liu Yinhao Li Binlin Shi Xiaoyu Guo Yanli Zhao Yangdong Guo Sumei Yan | 2021 | Journal of Animal Science and Biotechnology2021，12，2： | 3 |
| 13 | Pt/onion-like fullerenes as catalyst for direct methanol fuel cell显示文摘Onion-like fullerenes synthesized by arc discharge in water were used as support of Pt nanoparticles as electrocatalytic materials for direct methanol fuel cell. Uniform platinum nanoparticles with the average diameter of about 4.3 nm were well dispersed on the surface of onion-like fullerenes by impregnation-reduction method. The morphologies and microstructures of the as-prepared composites were studied by means of XRD and TEM. Electrochemical analysis shows that this kind of nano material may be an excellent candidate to be used as the support of catalyst for methanol electrochemical oxidation. | GUO Junjie YANG Xiaowei YAO Yanli WANG Xiaomin LIU Xuguang XU Bingshe | 2006 | Rare Metals2006，25，z1： | 3 |
| 14 | Expression of CDC42 in cervical squamous cell carcinoma and its correlation with clinicopathologic characteristics显示文摘Objective:The high expression of cell division cycle 42 protein(CDC42)may be involved in the occurrence and progression of several tumors.However,the expression and function of CDC42 in cervical squamous cell carcinoma remains unclear.This study aimed to investigate the expression of CDC42 in cervical squamous cell carcinoma and its correlation with clinicopathologic characteristics.Methods:The expression of CDC42 in 162 cervical squamous cell carcinoma tissue samples and 33 normal cervical tissue samples was investigated by immunohistochemistry.The CDC42 mRNA expression was detected by reverse transcription-polymerase chain reaction(RT-PCR).Results:ThecervicalsquamouscellcarcinomagroupshowedasignificantlyhigherCDC42positiverate,compared to the normal cervical tissues(P<0.05).Furthermore,the tissues of stage II-IV carcinoma patients showed higher CDC42 expression levels compared to stage I patients(P=0.05).In addition,the expression of CDC42 was not correlated to age of patients,differentiation degree of cancer cells,or lymph node metastasis(P>0.05).Furthermore,compare with normal cervical tissues,the CDC42 mRNAexpressionincervicalcancerhadnosignificantdifference.Conclusions:CDC42 was up-regulated at protein level,but not mRNA level,in cervical squamous cell carcinoma.The high expression of CDC42 was correlated to the clinical stage of the patients,indicating that CDC42 might contribute to the progression of cervical squamous cell carcinoma. | Ding Ma Yuan Cheng Youyi Zhang Yanli Guo Zijian Li Geng Li | 2013 | Chinese Journal of Cancer Research2013，25，6： | 3 |
| 15 | Structure-activity relationship optimization for lassa virus fusion inhibitors targeting the transmembrane domain of GP2显示文摘Dear Editor,Lassa virus(LASV)belongs to the Mammarenavirus genus,Arenaviridae family.Arenaviruses are classified into two main groups-Old World(OW)and New World(NW)-based on virus genetics,serology,antigenic properties and geographical relationships.The OW LASV and Lujo virus(LUJV),as well as NW Jurn'n virus(JUNV),Machupo virus(MACV),Guanarito virus(GTOV),Sabia virus(SABV)and Chapare virus(CHAPV),are known to cause severe hemorrhagic fever and are listed as biosafety level 4(BSL-4)agents.The arenavirus glycoprotein complex(GPC)contains three subunits-the retained stable-signal peptide(SSP),the receptor-binding subunit GP1,and the membrane fusion subunit GP2(Lenz et al.,2001).Notably,the proximate external membrane region and TM of GP2,together with the ectodomain loop and TMs of SSP,form an SSP-GP2 interface,playing essential roles in regulating membrane fusion,and providing targets for distinct fusion inhibitors(Larson et al.,2008;Lee et al.,2008;York et al.,2008;York and Nunberg,2009;Thomas et al.,2011;Burgeson et al.,2013a;Shankar et al.,2016;Wang et al.,2016;Wang et al.,2018).Among these inhibitors,ST-161 is LASV specific(Burgeson et al.,2013a).In this study,we conducted structure-activity relationship(SAR)optimization of ST-161.As a result,21 derivatives with IC50 values<1 pmol/L are presented in Table S1.Hit compounds 21,29 and 57 exhibiting robust inhibition of the LASV pseudotype virus(LASVpv,VSV backbone enveloped by LASV GPC with single cycle infection)entry with IC50 values lower than 0.2 nmol/L(Figs.1A and S1),as well as hit compound 72 with an ester bond instead of acylhydrazone,were further investigated.To test whether the four hit compounds inhibit LASV entry by blocking the GPC-mediated membrane fusion,the inhibition effects of these compounds against LASV GPC mediated fusion were quantitatively determined by dual-luciferase assay(Thomas et al.,2011;Wang et al.,2018). | Guangshun Zhang Junyuan Cao Yan Cai Yang Liu Yanli Li Peilin Wang Xiaoying Jia Mengmeng Zhang Gengfu Xiao Yu Guo Wei Wang | 2019 | Protein & Cell2019，10，2： | 3 |
| 16 | Cationic conjugated polymers as signal reporter for label-free assay based on targets-mediated aggregation of perylene diimide quencher显示文摘Herein, we reported a new label-free and fluorescence turn-on biosensor based on cationic conjugated poly(9,9-bis(6'-N,N,N-trimethylammonium)hexyl)fluorine phenylene)(PFP) and perylene diimide derivatives(PDI). Cationic PFP, single-stranded nucleic acid and PDI were used as signal reporter, probe and fluorescence quencher, respectively. In the presence of nucleic acids, they form complexes with PFP and PDI through strong electrostatic attraction interactions, resulting in PDI aggregating on nucleic acids and fluorescence of PFP being quenched. When nucleic acids are hydrolyzed by enzymes or their conformation is changed via recognizing targets, the effective aggregation of PDI is disrupted and the quenching ability is decreased. Thus the fluorescence of PFP recovers significantly. By taking advantage of the mechanism, we construct a new biosensor for endonuclease and small molecules detection. Here, S1 nuclease and bisphenol A are used as model systems. The detection limit of the SI nuclease and BPA are1.0×10^(-6)U/mL and 0.05 ng/mL, respectively. Our method is sensitive, cost-effective and simple, and provides a new platform for bioanalysis. | Limin Guo Ziqi Zhang Yanli Tang | 2018 | Chinese Chemical Letters2018，29，2： | 2 |
| 17 | Determination of optimal dietary selenium levels by full expression of selenoproteins in various tissues of broilers from 1 to 21 d of age显示文摘The current NRC dietary selenium(Se)requirement(0.15 mg/kg)of broilers is primarily based on growth performance data reported in 1986.Our study aimed to determine optimal dietary Se levels of broilers fed a practical corn-soybean meal diet for the full expression of selenoproteins in various tissues.A total of 384 one-d-old male broilers(n=8 replicates/diet)were fed a basal corn-soybean meal diet or the basal diet supplemented with 0.1,0.2,0.3,0.4 or 0.5 mg Se/kg in the form of Na_(2)SeO_(3) for 21 d.Regression analysis was conducted to evaluate the optimal dietary Se levels using broken-line,quadratic or asymptotic models.The activity of glutathione peroxidase(GPX)in the plasma,liver,kidney and pancreas,iodothyronine deiodinase(DIO)in the plasma,liver and pancreas,and thioredoxin reductase(Txnrd)in the liver and pancreas,the mRNA levels of Gpx1,Gpx4,Dio1,selenoprotein(Seleno)h,Selenop and Selenou in the liver,Gpx4,Dio1,Txnrd1,Txnrd2,Selenoh,Selenop and Selenou in the kidney,and Gpx1,Gpx4,Selenoh and Selenou in the pancreas,and the protein levels of GPX4 in the liver and kidney of broilers were influenced(P<0.05)by added Se levels,and increased quadratically(P<0.05)with the increase of added Se levels.The estimates of optimal dietary Se levels were 0.07 to 0.36 mg/kg based on the fitted broken-line,quadratic or asymptotic models(P<0.001)of the aforementioned selenoprotein expression in the plasma,liver and kidney,and 0.09 to 0.46 mg/kg based on the fitted broken-line models(P<0.001)of the aforementioned selenoprotein expression in the pancreas.The results indicate that the optimal dietary Se levels would be 0.36 mg/kg to support the full expression of selenoproteins in the plasma,liver and kidney,and 0.46 mg/kg to support the full expression of selenoproteins in the pancreas of broilers fed a practical corn-soybean meal diet from 1 to 21 d of age. | Xiudong Liao Guoqing Liu Guangming Sun Xiaoming Sun Tao Liu Lin Lu Liyang Zhang Minhong Zhang Yanli Guo Xugang Luo | 2021 | Animal Nutrition2021，，4： | 2 |
| 18 | Construction of on-line supercritical fluid extraction with reverse phase liquid chromatography–tandem mass spectrometry for the determination of capsaicin显示文摘A novel on-line system composed of supercritical fluid extraction(SFE), dilution line and reverse phase liquid chromatography/mass spectrometry(RPLC/MS) was constructed for on-line extraction and reverse phase separation of some fat-soluble components in foods. Three columns including a trap column,concentration column and analytical column were used for trapping the fat-soluble components, on-line enrichment and reverse phase separation, respectively. Capsaicinoids were on-line extracted by a CO_2 supercritical fluid, then concentrated and separated by using the C_(18) columns, finally detected by mass spectrometry(MS). Capsaicin eluted at 10.1 min and limit of detection(LOD, S/N=3) for the standard solution is 0.55pg. The linearity was calculated with a value of coefficient of determination(R^2)≥0.998 in the range of 1.1–8.5 ng. Concentrations of capsaicin in the green, yellow, and red bell peppers were determined to be 60.33 ng/g, 31.79 ng/g, 35.38ng/g, respectively. | Naoki Hamada Yuki Hashi Satoshi Yamaki Yanli Guo Lin Zhang Haifang Li Jin-Ming Lin | 2019 | Chinese Chemical Letters2019，30，1： | 2 |
| 19 | Expression of Pseudorabies Virus gE Core Epitopes in Escherichia coli Strain BL21 and Utilization of Indirect PRV gE-ELISA显示文摘Pseudorabies virus glycoprotein E( PRV gE) has been recognized as a suitable diagnostic antigen for pseudorabies. In order to produce gE antigen in large quantities and at low cost,a gene fragment encoding PRV gE core epitopes was expressed in E. coli BL21 expression system. SDS-PAGE and Western Blotting revealed that the expression product in culture supernatant of E. coli BL21 was a recombinant protein,approximately 51. 8 Kd. At 5 h post-induction,protein concentration assay showed that the expression product amounted to 1. 65 mg /ml,accounting for 24. 17% of total proteins in the culture supernatant. An indirect PRV gE-ELISA was established by using the recombinant expression product as a coating antigen. Cross-reactivity assay showed that this antigen was PRV specific. In addition,the assay was consistently reproducible. Comparison of detection results of 240 serum samples between PRV gE-ELISA and a commercially available PRV diagnostic kit showed that there was no significant difference between these two methods( P > 0. 05). | Guangjun GUO Sufang LU Guanggang QU Feng LI Lin DONG Yanli BI Jinliang WANG Feng WEI Na TANG Chunling ZHANG Zhuang DING Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，4： | 2 |
| 20 | Influence of Surface Nanocrystallization on Ti Ion Implantation of Pure Iron显示文摘In order to increase the depth or concentration of Ti ion implantation of pure iron, the surface mechanical attrition treatment(SMAT), which can fabricate a nanometer-grained surface layer without porosity and contamination in a pure iron plate, was used before ion implantation. Ti ion was implanted into the SMA treated sample and coarse-grained counterpart by using a metal vapor vacuum arc source implanter. The changing of depth and concentration of Ti was studied in a function of implantation time.By optical microscopy, transmission electron microscopy and X-ray diffraction, the grain size of the nano structured surface was studied. Micro-hardness, friction and wear behavior of nano surface layers were studied. By energy dispersive X-ray spectroscopy and Auger electron spectroscopy, the chemical composition and concentration of Ti ion in the surface implantation layer were studied. Experimental results showed that the concentration of Ti increased dramatically compared with untreated coarsegrained samples, which is attributed to the existence of higher density of defects(supersaturated vacancies, dislocations, non-equilibrium grain boundaries etc.) and compression stress field in the SMA treated nanocrystallined surface layer. The interaction between the defects and the implanted solute atoms leads to the increment of solid solubility. But the implantation depth showed inconspicuous change. It is shown that the ion range is just relevant to the energy and mass of the ion, dose of injection,the mass and density of target material. | Xu Li Yanli An Yinghui Wei Huayun Du Lifeng Hou Chunli Guo Hongbo Qu Yide Wang | 2015 | Journal of Materials Science & Technology2015，31，3： | 2 |
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