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| 1 | Plant-derived phosphocholine facilitates cellular uptake of anti-pulmonary fibrotic HJT-sRNA-m7显示文摘Pulmonary fibrosis, a progressive chronic disease with a high mortality rate, has limited treatment options. Currently, lung transplantation remains the only effective treatment. Here we report that a small RNA, HJT-sRNA-m7, from a Chinese herbal medicine Hong Jing Tian(HJT, RHODIOHAE CRENULATAE RADIX ET RHIZOMA, Rhodiola crenulata) can effectively reduce the expressions of fibrotic hallmark genes and proteins both in alveolar in vitro and in mouse lung tissues in vivo. We also discovered over one hundred oil-soluble chemicals from HJT decoctions, most of which are found in lipid extracts from other Chinese herbals decoctions, including Pu Gong Ying(PGY, TARAXACI HERBA, Taraxacum mongolicum), Chuan Xin Lian(CXL, changed to 'ANDROGRAPHIS HERBA, Andrographis paniculata'), and Jin Yin Hua(JYH, lonicera japonica or Honeysuckle). We identified the active component in these decoctions as two forms of phosphocholines, PC(18:0/18:2) and PC(16:0/18:2). These PCs potentially could form liposomes with small RNAs to enter human alveolar and gastric cells. Our experimental results suggest an unprecendent lipid complex route through which botanic small RNA can enter human bodies.Our results provide an innovative treatment strategy for oral delivery of siRNAs as therapeutic medication. | Jianchao Du Zhu Liang Jiantao Xu Yan Zhao Xiaoyun Li Yanli Zhang DANDan Zhao Ruxuan Chen Yang Liu Trupti Joshi Jiahui Chang Zhiqing Wang Yanxu Zhang Jindong Zhu Qiang Liu Dong Xu Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 21 |
| 2 | Flavonoid profiling of a traditional Chinese medicine formula of Huangqin Tang using high performance liquid chromatography显示文摘The quality control processes for herbal medicines have been problematic. Flavonoids are the major active components of Huangqin Tang(HQT, a traditional Chinese medicine formula). In this study, we used a combinative method approach consisting of chromatographic fingerprinting(high performance liquid chromatography; HPLC), quantitative methods and a pharmacodynamic evaluation model to analyze the flavonoids of HQT obtained from different sources. Ten batches of HQT were analyzed by the HPLC fingerprinting method and 26 common peaks were detected, of which 23 peaks corresponded with the chemical profile of HQT. In addition, 11 major compounds were identified by LC–MS analysis(liquid chromatography–tandem mass spectrometer; LC–MS^n) and quantified by the HPLC quantitative method approach. The studied10 batches of HQT were found to be homogeneous in their composition with a similarity between 0.990 and1.000. The distribution of the 11 identified compounds was found to be very similar among the batches. Only slight pharmacodynamic differences were detected between the different batches, confirming the homogeneity of HQT. The results of this study prove that the combination of chromatographic fingerprinting and quantitative analysis can be readily used for comprehensive quality control of herbal medicines. | Tao Li Shuaixing Zhuang Yiwei Wang Yanli Wang Weihao Wang Huihui Zhang Li Chen Dunfang Wang Zhongming Zhou Weipeng Yang | 2016 | Acta Pharmaceutica Sinica B2016，6，2： | 19 |
| 3 | Characteristics of atmospheric single particles during haze periods in a typical urban area of Beijing:A case study in October,2014显示文摘To investigate the composition and possible sources of particles, especially during heavy haze pollution, a single particle aerosol mass spectrometer(SPAMS) was deployed to measure the changes of single particle species and sizes during October of 2014, in Beijing. A total of 2,871,431 particles with both positive and negative spectra were collected and characterized in combination with the adaptive resonance theory neural network algorithm(ART-2a). Eight types of particles were classified: dust particles(dust, 8.1%), elemental carbon(EC, 29.0%), organic carbon(OC, 18.0%), EC and OC combined particles(ECOC, 9.5%),Na-K containing particles(Na K, 7.9%), K-containing particles(K, 21.8%), organic nitrogen and potassium containing particles(KCN, 2.3%), and metal-containing particles(metal,3.6%). Three haze pollution events(P1, P2, P3) and one clean period(clean) were analyzed,based on the mass and number concentration of PM_(2.5)and the back trajectory results from the hybrid single particle Lagrangian integrated trajectory model(Hysplit-4 model). Results showed that EC, OC and K were the major components of single particles during the three haze pollution periods, which showed clearly increased ratios compared with those in the clean period. Results from the mixing state of secondary species of different types of particles showed that sulfate and nitrate were more readily mixed with carbon-containing particles during haze pollution episodes than in clean periods. | Lang Liu Yanli Wang Shiyong Du Wenjie Zhang Lujian Hou Sverre Vedal Bin Han Wen Yang Mindong Chen Zhipeng Bai | 2016 | Journal of Environmental Sciences2016，28，2： | 19 |
| 4 | Influence of Gd_2O_3 and Yb_2O_3 Co-doping on Phase Stability,Thermo-physical Properties and Sintering of 8YSZ显示文摘The role of multicomponent rare earth oxides in phase stability, thermophysical properties and sintering for ZrO2-based thermal barrier coatings (TBCs) materials is investigated. 8YSZ codoped with 3 mol% Gd2O3 and 3 mol% Yb2O3 (GYb-YSZ) powders are synthesized by solid state reaction for 24 h at various temperatures. As temperature increases, stabilizers are dissolved into zirconia matrix gradually. Synthesized at 1 500 °C, GYb-YSZ is basically composed of cubic phase. GYb-YSZ exhibits excellent phase stability and sinters lower than 8YSZ by nearly three times. The thermal conductivity of GYb-YSZ is much lower than that of 8YSZ, and the thermal expansion coefficient of GYb-YSZ is comparable to that of 8YSZ. The influence of Gd2O3 and Yb2O3 co-doping on phase stability, thermal conductivity and sintering of 8YSZ is discussed. | ZHANG Yanli GUO Lei YANG Yongping GUO Hongbo ZHANG Hongju GONG Shengkai | 2012 | Chinese Journal of Aeronautics2012，25，6： | 17 |
| 5 | SUMO E3 Ligase AtMMS21 Regulates Drought Tolerance in Arabidopsis thaliana显示文摘Post-translational modifications of proteins by small ubiquitin-like modifiers (SUMOs) play crucial roles in plant growth and development, and in stress responses. The MMS21 is a newly-identified Arabidopsis thaliana L. SUMO E3 ligase gene aside from the SIZ1, and its function requires further elucidation. Here, we show that MMS21 deficient plants display improved drought tolerance, and constitutive expression of MMS21 reduces drought tolerance. The expression of MMS21 was reduced by abscisic acid (ABA), polyethylene glycol (PEG) or drought stress. Under drought conditions, mms21 mutants showed the highest survival rate and the slowest water loss, and accumulated a higher level of free proline compared to wild-type (WT) and MMS21 over-expression plants. Stomatal aperture, seed germination and cotyledon greening analysis indicated that mms21 was hypersensitive to ABA. Molecular genetic analysis revealed that MMS21 deficiency led to elevated expression of a series of ABA-mediated stress-responsive genes, including COR15A, RD22, and P5CS1 The ABA and drought-induced stress-responsive genes, including RAB18, RD29A and RD29B, were inhibited by constitutive expression of MMS21. Moreover, ABA-induced accumulation of SUMO-protein conjugates was blocked in the mms21 mutant. We thus conclude that MMS21 plays a role in the drought stress response, likely through regulation of gene expression in an ABA-dependent pathway. | Shengchun Zhang Yanli Qi Ming Liu Chengwei Yang | 2013 | Journal of Integrative Plant Biology2013，55，1： | 17 |
| 6 | Attribute-Based Access Control for Multi-Authority Systems with Constant Size Ciphertext in Cloud Computing显示文摘In most existing CP-ABE schemes, there is only one authority in the system and all the public keys and private keys are issued by this authority, which incurs ciphertext size and computation costs in the encryption and decryption operations that depend at least linearly on the number of attributes involved in the access policy. We propose an efficient multi-authority CP-ABE scheme in which the authorities need not interact to generate public information during the system initialization phase. Our scheme has constant ciphertext length and a constant number of pairing computations. Our scheme can be proven CPA-secure in random oracle model under the decision q-BDHE assumption. When user's attributes revocation occurs, the scheme transfers most re-encryption work to the cloud service provider, reducing the data owner's computational cost on the premise of security. Finally the analysis and simulation result show that the schemes proposed in this thesis ensure the privacy and secure access of sensitive data stored in the cloud server, and be able to cope with the dynamic changes of users' access privileges in large-scale systems. Besides, the multi-authority ABE eliminates the key escrow problem, achieves the length of ciphertext optimization and enhances the effi ciency of the encryption and decryption operations. | CHEN Yanli SONG Lingling YANG Geng | 2016 | China Communications2016，13，2： | 15 |
| 7 | Intent Pattern Recognition of Lower-limb Motion Based on Mechanical Sensors显示文摘Based on the regularity nature of lower-limb motion,an intent pattern recognition approach for above-knee prosthesis is proposed in this paper. To remedy the defects of recognizer based on electromyogram(EMG), we develop a pure mechanical sensor architecture for intent pattern recognition of lower-limb motion. The sensor system is composed of an accelerometer, a gyroscope mounted on the prosthetic socket, and two pressure sensors mounted under the sole. To compensate the delay in the control of prosthesis, the signals in the stance phase are used to predict the terrain and speed in the swing phase. Specifically, the intent pattern recognizer utilizes intraclass correlation coefficient(ICC) according to the Cartesian product of walking speed and terrain. Moreover, the sensor data are fused via DempsterShafer's theory. And hidden Markov model(HMM) is used to recognize the realtime motion state with the reference of the prior step. The proposed method can infer the prosthesis user's intent of walking on different terrain, which includes level ground,stair ascent, stair descent, up and down ramp. The experiments demonstrate that the intent pattern recognizer is capable of identifying five typical terrain-modes with the rate of 95.8%. The outcome of this investigation is expected to substantially improve the control performance of powered above-knee prosthesis. | Zuojun Liu Wei Lin Yanli Geng Peng Yang | 2017 | IEEE/CAA Journal of Automatica Sinica2017，4，4： | 15 |
| 8 | Curative effect of acupuncture and moxibustion on insomnia: a randomized clinical trial显示文摘OBJECTIVE: To observe the effect of acupuncture and moxibustion on insomnia and explore its mechanism. METHODS: One hundred and twenty patients were randomly divided into an experiment group and a control group. Sixty patients in the experiment group were treated once a day with acupuncture at Baihui (GV 20), Sishencong (EX-HN 1), Shenmai (BL 62), and Zhaohai (KI 6) and with moxibustion at Baihui (GV 20) and Sishencong (EX-HN 1). Sixty patients in the control group were acupunctured once a day at Shenmen (HT 7), Neiguan (PC 6), and Sanyinjiao (SP 6).The Pittsburgh Sleep Quality Index (PSQI) was used to compare sleep improvement between the two groups. RESULTS: The total effective rate was 87.7% in the experiment group and 76.3% in the control group. The PSQI scores and the total score were lower after treatment than before treatment in bothgroups. However, the reduction in the experiment group was greater than that in the control group in sleeping quality, time to fall asleep, sleeping disorder, and daytime function (P<0.05). CONCLUSION: Acupuncture and moxibustion at Baihui (GV 20), Sishencong (EX-HN 1), Shenmai (BL 62), and Zhaohai (KI 6) significantly improved insomnia symptoms in the experiment group compared with the control group. | Xiyan Gao Cuixiang Xu Peiyu Wang Shan Ren Yanli Zhou Xuguang Yang Ling Gao | 2013 | Journal of Traditional Chinese Medicine2013，33，4： | 14 |
| 9 | Tracing the first hematopoietic stem cell generation in human embryo by single-cell RNA sequencing显示文摘Tracing the emergence of the first hematopoietic stem cells(HSCs)in human embryos,particularly the scarce and transient precursors thereof,is so far challenging,largely due to the technical limitations and the material rarity.Here,using single-cell RNA sequencing,we constructed the first genome-scale gene expression landscape covering the entire course of endothelial-to-HSC transition during human embryogenesis.The transcriptomically defined HSC-primed hemogenic endothelial cells(HECs)were captured at Carnegie stage(CS)12-14 in an unbiased way,showing an unambiguous feature of arterial endothelial cells(ECs)with the up-regulation of RUNX1,MYB and ANGPTL Importantly,subcategorizing CD34+CD45-ECs into a CD44+population strikingly enriched HECs by over 10-fold.We further mapped the developmental path from arterial ECs via HSC-primed HECs to hematopoietic stem progenitor cells,and revealed a distinct expression pattern of genes that were transiently over-represented upon the hemogenic fate choice of arterial ECs#including EMCN,PROCR and RUNX1T1.We also uncovered another temporally and molecularly distinct intra-embryonic HEC population,which was detected mainly at earlier CS 10 and lacked the arterial feature.Finally,we revealed the cellular components of the putative aortic niche and potential cellular interactions acting on the HSCprimed HECs.The cellular and molecular programs that underlie the generation of the first HSCs from HECs in human embryos,together with the ability to distinguish the HSC-primed HECs from others,will shed light on the strategies for the production of clinically useful HSCs from pluripotent stem cells. | Yang Zeng Jian He Zhijie Bai Zongcheng Li Yandong Gong Chen Liu Yanli Ni Junjie Du Chunyu Ma Lihong Bian Yu Lan Bing Liu | 2019 | Cell Research2019，29，11： | 14 |
| 10 | Theory and Method of Power System Integrated Security Region Irrelevant to Operation States:An Introduction显示文摘How to comprehensively consider the power flow constraints and various stability constraints in a series of power system optimization problems without affecting the calculation speed is always a problem.The computational burden of probabilistic security assessment is even more unimaginable.In order to solve such problems,a security region(SR)methodology is proposed,which is a brand-new methodology developed on the basis of the classical point-wise method.Tianjin University has been studying the SR methodology since the 1980s,and has achieved a series of original breakthroughs that are described in this paper.The integrated SR introduced in this paper is mainly defined in the power injection space,and includes SRs to ensure steady-state security,transient stability,static voltage stability,and smalldisturbance stability.These SRs are uniquely determined for a given network topology(as well as location and clearing process for transient faults)and given system component parameters,and are irrelevant to operation states.This paper presents 11 facts and related remarks to introduce the basic concepts,composition,dynamics nature,and topological and geometric characteristics of SRs.It also provides a practical mathematical description of SR boundaries and fast calculation methods to determine them in a concise and systematic way.Thus,this article provides support for the systematic understanding,future research,and applications of SRs.The most critical finding on the topological and geometric characteristics of SRs is that,within the scope of engineering concern,the practical boundaries of SRs in the power injection space can be approximated by one or a few hyperplanes.Based on this finding,the calculation time for power system probabilistic security assessment(i.e.,risk analysis)and power system optimization with security constraints can be decreased by orders of magnitude. | Yixin Yu Yanli Liu Chao Qin Tiankai Yang | 2020 | Engineering2020，6，7： | 10 |
| 11 | Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry显示文摘An efficient method for the simultaneous extraction and quantification of both jasmonic acid(JA)and methyl jasmonate(MeJA) from a single plant sample was developed.Plant tissues were firstly extracted by using 100%cold methanol,then the Sep-pak C18 solid-phase extraction(SPE)cartridges were adopted for the purification of sample extract,and finally JA and MeJA were determined by the liquid chromatography tandem mass spectrometry(LC-MS-MS)system.It was found that the accuracy was improved by using the extra standard for the estimation and correction of JA and MeJA losses in the extraction and purification process.The detection limits for JA and MeJA were 0.03 and 0.075 ng mL-1,respectively,and the average recovery rate of JA and MeJA was 92.48%and 94.30%.The method was found to be reproducible and selective,yielding well isolated and easily detectable peaks for both JA and MeJA and simplifying the time-consuming extraction and purification.High-performance liquid chromatography(HPLC)system was employed as the control detection method for the LC-MS-MS system.The two systems were compared in their specificity and accuracy. | LIU Xia YANG YanLi LIN WanHuang TONG JianHua HUANG ZhiGang XIAO LangTao | 2010 | Chinese Science Bulletin2010，55，21： | 10 |
| 12 | Simple synthesis of a porous Sb/Sb2O3 nanocomposite for a high-capacity anode material in Na-ion batteries显示文摘高能力的阳极材料为钠离子电池是高度合乎需要的。这里,多孔的 Sb/Sb 2 O 3 nanocomposite 被 Sb nanocrystals 的温和氧化成功地在空中综合。在里面合成, Sb 贡献好电导率和 Sb 2 O 3 改进骑车的稳定性,特别地在 0.02-1.5 V 的电压窗户以内。它在 540 mAh 的一个可逆能力留下 ??? ′ | Jun Pan Nana Wang Yanli Zhou Xianfeng Yang Wenyao Zhou Yitai Qian Jian Yang | 2017 | Nano Research2017，10，5： | 9 |
| 13 | Status and development of high-power laser facilities at the NLHPLP显示文摘In this paper, we review the status of the multifunctional experimental platform at the National Laboratory of High Power Laser and Physics(NLHPLP). The platform, including the SG-II laser facility, SG-II 9th beam, SG-II upgrade(SG-II UP) facility, and SG-II 5 PW facility, is operational and available for interested scientists studying inertial confinement fusion(ICF) and a broad range of high-energy-density physics. These facilities can provide important experimental capabilities by combining different pulse widths of nanosecond, picosecond, and femtosecond scales. In addition, the SG-II UP facility, consisting of a single petawatt system and an eight-beam nanosecond system, is introduced including several laser technologies that have been developed to ensure the performance of the facility. Recent developments of the SG-II 5 PW facility are also presented. | Jianqiang Zhu Jian Zhu Xuechun Li Baoqiang Zhu Weixin Ma Xingqiang Lu Wei Fan Zhigang Liu Shenlei Zhou Guang Xu Guowen Zhang Xinglong Xie Lin Yang Jiangfeng Wang Xiaoping Ouyang Li Wang Dawei Li Pengqian Yang Quantang Fan Mingying Sun Chong Liu Dean Liu Yanli Zhang Hua Tao Meizhi Sun Ping Zhu Bingyan Wang Zhaoyang Jiao Lei Ren Daizhong Liu Xiang Jiao Hongbiao Huang Zunqi Lin | 2018 | High Power Laser Science and Engineering2018，6，4： | 8 |
| 14 | A Brief Introduction to BNU-HESM1.0 and Its Earth Surface Temperature Simulations显示文摘Integrated assessment models and coupled earth system models both have their limitations in understanding the interactions between human activity and the physical earth system. In this paper,a new human–earth system model,BNUHESM1.0,constructed by combining the economic and climate damage components of the Dynamic Integrated Model of Climate Change and Economy to the BNU-ESM model,is introduced. The ability of BNU-HESM1.0 in simulating the global CO2 concentration and surface temperature is also evaluated. We find that,compared to observation,BNU-HESM1.0underestimates the global CO2 concentration and its rising trend during 1965–2005,due to the uncertainty in the economic components. However,the surface temperature simulated by BNU-HESM1.0 is much closer to observation,resulting from the overestimates of surface temperature by the original BNU-ESM model. The uncertainty of BNU-ESM falls within the range of present earth system uncertainty,so it is the economic and climate damage component of BNU-HESM1.0 that needs to be improved through further study. However,the main purpose of this paper is to introduce a new approach to investigate the complex relationship between human activity and the earth system. It is hoped that it will inspire further ideas that prove valuable in guiding human activities appropriate for a sustainable future climate. | YANG Shili DONG Wenjie CHOU Jieming FENG Jinming YAN Xiaodong WEI Zhigang YUAN Wenping GUO Yan TANG Yanli HU Jiacong | 2015 | Advances in Atmospheric Sciences2015，32，12： | 8 |
| 15 | Experimental study on the mechanical properties and consolidation mechanism of microbial grouted backfill显示文摘Backfill mining technology is the practice of returning waste materials underground for both disposal and geotechnical stability,however,a challenge with current technologies is that they commonly require cement-based binders which have a relatively high environmental impact.Finding alternatives to cement-based binders can improve environmental performance and this paper proposes microbial grouted backfill(MGB)as a potential solution.In this paper,the effects of the cementation solution concentration(CSC),volume ratio of bacterial solution to cementation solution(VRBC),particle sizes of the aggregates,and the number of grouting batches on the mechanical properties of MGB are studied.The experimental results show that MGB strength increased,up to a peak value,as CSC was increased,before decreasing as CSC was increased further.The results also show that MGB strength increased,up to a peak value,as VRBC decreased,before decreasing as the VRBC was decreased further.The peak strength was achieved at a CSC of 2 mol/L and a VRBC of 1:9.The strength of the MGB also increased as the number of grouting batches increased.Graded MGB samples showed the highest UCS,25.12 MPa,at particle sizes of 0.2 to 0.8 mm,while full(non-graded)MGB samples displayed mean UCS values ranging from1.56 MPa when the maximum particle size was 0.2 mm,up to 13 MPa when the maximum particle size was 1.2 mm.MGB samples are consolidated by the calcium carbonate that is precipitated during microbial metabolism,and the strength of MGB increases linearly as calcium carbonate content increases.The calcium carbonate minerals produced in MGB materials are primarily calcite,with secondary amounts of vaterite. | Xuejie Deng Yu Li Fei Wang Xiaoming Shi Yinchao Yang Xichen Xu Yanli Huang Benjamin de Wit | 2022 | International Journal of Mining Science and Technology2022，32，2： | 7 |
| 16 | Distribution and risk factors of hand, foot, and mouth disease in Changchun, northeastern China显示文摘Hand,foot,and mouth disease(HFMD)is a public health problem,and there have been increasing numbers of outbreaks in China's Mainland since 2008.Over17,000 HFMD cases have been reported in Changchun between 2008 and 2011.This study characterized the temporal and spatial distribution of the disease and identified the risk factors for HFMD.The main findings were as follows:(i)there were significant differences in HFMD incidence among age groups,with 86.8%of reported cases in children younger than 5 years old,and boys showed a higher incidence than girls(\6 years);(ii)The disease affected the whole region and spanned a large geographic area,but there was a higher incidence in urban areas(median=242 per 10,000 persons)and urban–rural border areas(median=135 per 10,000 persons),compared with rural areas(median=75 per 10,000 persons);and(iii)the incidence of HFMD in Changchun was significantly associated with the distance to the nearest freeway,GDP per capita,and the type of township. | Li Yan Xinlou Li Yaqin Yu Sake J.de Vlas Yapin Li Dandan Wang Yanli Li Yuan Yin Jing Wu Hong Liu Hong Yang Bo Li Liqun Fang Wuchun Cao | 2014 | Chinese Science Bulletin2014，59，5： | 7 |
| 17 | Longitudinal study of a community hospital integrated model for diabetes management in the Beijing Jingsong community显示文摘Objective:To determine the effects of a community hospital integrated model on the longitu-dinal management of diabetic patients.Methods:Four hundred forty-one patients with type 2 diabetes residing in Jingsong Commu-nity were randomly assigned to intensive and standard groups.Metabolic parameters were meas-ured in the two groups at baseline and after 36 months of management to compare the rate of goal achievement.Results:After comprehensive management,the overall control rate in the intensive group was increased from 2.7%to 9.6%compared with 2.3%-4.5%in the standard group.Specifically,the control rates for fasting plasma glucose,glycosylated hemoglobin,and blood pressure in the intensive group were significantly increased from 54.1%,40.0%,and 68.2%to 85.5%,74.6%,and 89.1%,respectively.The control rate for glycosylated hemoglobin in the intensive group was significantly higher than the standard group after 36 months of treatment.Conclusion:The community hospital integrated model for longitudinal management effec-tively improved the control rate of glycosylated hemoglobin and the overall control rate in patients with type 2 diabetes. | Jiandong Zhang Ning Zhuang Mingxia Yuan Lijuan Gao Chuan Bian Huaining Chen Qing Chi Suqing Hui Guiying Li Yanli Su Hongsong Bao Gang Wan Guangran Yang Shenyuan Yuan | 2014 | Family Medicine and Community Health2014，2，1： | 7 |
| 18 | Intestinal toxicity of deoxynivalenol is limited by supplementation with Lactobacillus plantarum JM113 and consequentially altered gut microbiota in broiler chickens显示文摘Background: Limited research has focused on the effect of Lactobacillus on the intestinal toxicity of deoxynivalenol(DON).The present study was conducted to investigate the role of Lactobacillus plantarum(L.plantarum) JM113 in protecting against the intestinal toxicity caused by DON.Methods: A total of 144 one-day-old healthy Arbor Acres broilers were randomly distributed into 3 treatments,including the CON(basal diet),the DON(extra 10 mg/kg deoxynivalenol),and the DL(extra 1 × 109 CFU/kg L.plantarum JM113 based on DON group) treatments.The growth performance,organ indexes,intestinal morphology,pancreatic digestive enzymes,intestinal secreted immunoglobulin A(sIgA),jejunal transcriptome,and intestinal microbiota were evaluated.Results: Compared with the CON and DL groups,the DON supplementation altered intestinal morphology,especially in duodenum and jejunum,where villi were shorter and crypts were deeper(P < 0.05).Meanwhile,the significantly decreased mRNA expression of jejunal claudin-1 and occludin(P < 0.05),ileal rBAT and jejunal GLUT1 of 21-day-old broilers(P < 0.05),as well as duodenal PepT1 and ileal rBAT of 42-day-old broilers were identified in the DON group.Moreover,supplementation with L.plantarum JM113 could increase duodenal expression of IL-10 and IL-12 of 21-dayold broilers,ileal s IgA of 42-day-old broilers,and the bursa of Fabricius index of 21-day-old broilers.Further jejunal transcriptome proved that the genes related to the intestinal absorption and metabolism were significantly reduced in the DON group but a significant increase when supplemented with extra L.plantarum JM113.Furthermore,the bacteria related to nutrient utilization,including the Proteobacteria,Escherichia,Cc-115(P < 0.05),Lactobacillus and Prevotella(P < 0.1) were all decreased in the DON group.By contrast,supplementation with L.plantarum JM113 increased the relative abundance of beneficial bacterium,including the Bacteroidetes,Roseburia,Anaerofustis,Anaerostipe,and Ruminococcus bromi(P < 0.05).Specifically,the increased abundance of bacteria in the DL group could be proved by the significantly increased caecal content of propionic acid,n-Butyric acid,and total short-chain fatty acid.Conclusions: L.plantarum JM113 enhanced the digestion,absorption,and metabolic functions of the gut when challenged with DON by reducing the injury to intestinal barriers and by increasing the abundance of beneficial bacterium. | Shengru Wu Yanli Liu Yongle Duan Fangyuan Wang Fangshen Guo Fang Yan Xiaojun Yang Xin Yang | 2019 | Journal of Animal Science and Biotechnology2019，10，1： | 7 |
| 19 | Molecular Cytogenetic Characterization of a Wheat-Leymus mollis 3D(3Ns) Substitution Line with Resistance to Leaf Rust显示文摘Leymus mollis(Trin.)Pilger(NsNsXmXm,2n?28),a wild relative of common wheat,possesses many potentially valuable traits that could be transferred to common wheat during breeding programs.In this study,the karyotypic constitution of a wheat e L.mollis 3D(3Ns#1)disomic substitution line isolated from the F5progeny of octoploid Tritileymus M842-16 Triticum durum cv.D4286,which was designated as 10DM57,was determined using genomic in situ hybridization(GISH),fluorescent in situ hybridization(FISH),SSR markers,and ESTSTS markers.Screening of mitosis and meiosis showed that 10DM57 had a chromosome karyotype of 2n?42?21II.GISH indicated that10DM57 was a line with 40 chromosomes from wheat and two of the Ns chromosomes from L.mollis,which formed a ring bivalent in pollen mother cells at metaphase I.FISH analysis showed that the chromosome 3D may be replaced by 3Ns#1 in 10DM57.DNA markers,including SSR and EST-STS primers,showed that the pair of wheat chromosome 3D in 10DM57 was substituted by the pair of chromosome 3Ns#1from L.mollis.Evaluation of the agronomic traits showed that,compared with its common wheat relative 7182,10DM57 was resistant to leaf rust while the spike length and number of spikes per plant were improved significantly,which correlated with a higher wheat yield.The new germplasm,10DM57,could be exploited as an intermediate material in wheat genetic and breeding programs. | Yuhui Pang Xinhong Chen Jixin Zhao Wanli Du Xueni Cheng Jun Wu Yanli Li Liangming Wang Jing Wang Qunhui Yang | 2014 | Journal of Genetics and Genomics2014，41，4： | 6 |
| 20 | Studies on Factors Influencing Hydrodynamic Characteristics of Plates Used in Artificial Reefs显示文摘As a simplified model of artificial reefs, a series of plate models punched with square or circular openings are designed to investigate the effects of openings on the hydrodynamic characteristics of artificial reefs. The models are grouped by various opening numbers and opening-area ratios. They are physically tested in a water flume or used in the numerical simulation to obtain the drag force in the uniform flow with different speeds. The simulation results are found in good agreement with the experimental measurements. By the non-dimensional analysis, the drag coefficient specified to each model is achieved and the effects of openings are examined. It is found that the key factor affecting the drag coefficient is the open-area ratio. Generally, the drag coefficient is a linear function of the open area ratio with a minus slope. The empirical formulae for the square and circular openings respectively are deduced by means of the multiple regression analysis based on the measured and numerical data. They will be good references for the design of new artificial reefs. As a result of numerical simulation, the vorticity contours and pressure distribution are also presented in this work to better understand the hydrodynamic characteristics of different models. | TANG Yanli YANG Wenzhao SUN Liyuan ZHAO Fenfang LONG Xiangyu WANG Gang | 2019 | Journal of Ocean University of China2019，18，1： | 6 |
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