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| 1 | Properties of Supersulphated Phosphogysum-slag Cement (SSC) Concrete显示文摘Supersulphated phosphogysum-slag cement(SSC) is a newly developed non-burned cementitious material mainly composed of phosphogysum(PG) and ground granulated blast furnace slag(GGBFS), with small amount of steel slag(SS) and clinker(CL). SSC is a kind of environmentally-friendly cementitious material due to its energy-saving, low-carbon emission, and waste-utilization. We prepared concretes with supersulphated phosphogysum-slag cement, and studied the mechanical properties, microproperties and resistance to chloride penetration of concrete in comparison with those of portland slag cement(PSC) and ordinary portland cement(OPC) concrete. The test results show that the compressive strength of SSC concrete can reach 38.6 MPa after 28 d, close to PSC concrete and OPC concrete. Microanalyses indicate that large quantities of ettringite and C-S-H, and little amount of Ca(OH)2 are generated during the hydration of SSC. The dense cement paste structure of SSC is formed by ettringite and C-S-H, surrounded unreacted phosphogysum. The property of resistance to chloride penetration of SSC concrete is better than PSC and OPC concrete due to the fact that SSC can form much more ettringite to solidify more Cl-. | 丁沙 水中和 CHEN Wei LU Jianxin TIAN Sufang | 2014 | Journal of Wuhan University of Technology(Materials Science)2014，29，1： | 6 |
| 2 | The essential oil components of Cinnamomum cassia: an analysis under different thinning models of plantation Pinus massoniana显示文摘Thinning is an important activity employed in forest management. To date, studies have mainly focused on the effects of thinning on the growth of trees during the same thinning period. In this study, plantation Pinus massoniana Lamb. near maturity were thinned at varying intensities and an economically important species, Cinnamomum cassia Presl., was planted beneath the thinned canopy. The aim of the study was to explore the effects of the extent of thinning on the essential oil content and its components of C. cassia in different parts of the plant, as well as the economic feasibility of the P. massoniana–C.cassia management model. Thinning significantly reduced the oil yield in the bark and branches of C. cassia, but hardly impacted the oil yield from C. cassia leaves compared with pure C. cassia forest(CK). Among the different thinning treatments, both light(T.4) and extensive(T.1)thinning reduced the oil yield of C. cassia bark and new branches. The concentrations of the main aldehydes differed in different parts of the plant and were affected by the extent of thinning. The influence on cinnamaldehyde in the bark was minor, but was much greater in the branches and leaves. Both the oil yield and content of cinnamaldehyde showed that moderate(T.3) thinning was more favorable than other thinning models. These results not only provide a potentially promising model for the transformation of low–yield artificial pure forests of P. massoniana in the future, but also offer a reference for the management of artificial mixed stands. | Dayan Tao Yuanfa Li Daodiao Lu Yehong Luo Sufang Yu Shaoming Ye | 2016 | Journal of Forestry Research2016，27，3： | 5 |
| 3 | Identification of a Differentially-expressed Gene in Fatty Liver of Overfeeding Geese显示文摘响应 overfeeding,鹅得脂肪肝。理解肥育的机制, mRNA 微分显示反转录 PCR 被用来处于 overfeeding 并且正常的喂的条件学习法语的 Landes 灰鹅和 Xupu 白鹅之间的基因表达差别。一基因被发现在在两个品种的脂肪肝起来调整,并且它与83%身份有 1797 bp cDNA 到揭示的鸡 SELENBP1.The 顺序分析它 1413 bp 的开的读物框架编码 471 氨基酸的蛋白质,它与高相同包含 56 kDa 硒绑定蛋白质的通常认为的保存的域到它鸡肉(95%)的相当或相同事物,老鼠(86%),老鼠(84%),人(86%),猴子(86%),狗(86%),并且这蛋白质的牛(86%) .Thefunction 简短基于出版信息被考察了。在织物表达式分析,鹅 SELENBP1 mRNA 的表达式被发现比在另外的测试纸巾在肝或肾更高。结果证明 overfeeding 能增加鹅 SELENBP1 的 mRNA 表达式水平。 | Ayong ZHAO Huachun TANG Sufang LU Ruiguo HE | 2007 | Acta Biochimica et Biophysica Sinica2007，39，9： | 4 |
| 4 | Preparation of New Cementitious System using Fly Ash and Dehydrated Autoclaved Aerated Concrete显示文摘We experimentally studied the interaction between pozzolanic material(fly ash) and dehydrated autoclaved aerated concrete(DAAC). The DAAC powder was obtained by grinding aerated concrete waste to particles fi ner than 75μm and was then heated to temperatures up to 900 ℃. New cementitious material was prepared by proportioning fly ash and DAAC, named as AF. X-ray diffraction(XRD) was employed to identify the crystalline phases of DAAC before and after rehydration. The hydration process of AF was analyzed by the heat of hydration and non-evaporable water content(Wn). The experimental results show that the highest reactivity of DAAC can be obtained by calcining the powder at 700 ℃ and the dehydrated products are mainly β-C2 S and CaO. The cumulative heat of hydration and Wn was found to be strongly dependent on the replacement level of fl y ash, increasing the replacement level of fl y ash lowered them in AF. The strength contribution rates on pozzolanic effect of fl y ash in AF are always negative, showing a contrary tendency of that of cement-fl y ash system. | 水中和 LU Jianxin TIAN Sufang SHEN Peiliang DING Sha | 2014 | Journal of Wuhan University of Technology(Materials Science)2014，29，4： | 3 |
| 5 | Establishment and Application of a SYBR Green I Real-time Quantitative PCR Assay for the Detection of LAT Gene of Pseudorabies Virus in Bama Miniature Pigs显示文摘[Objective] This study aimed to establish a rapid,sensitive and specific SYBR Green I real-time quantitative PCR assay for the detection of latent pseudorabies virus( PRV) infection. [Method]SYBR Green I real-time quantitative PCR conditions and system for early detection of latent pseudorabies virus infection were optimized and compared with conventional PCR to investigate the sensitivity and specificity. Subsequently,the established assay was applied to detect different clinical samples. [Result]The sensitivity of SYBR Green I real-time quantitative PCR assay( 52 copies / μl) was 1 000 times higher than that of conventional PCR( 5. 2 × 104 copies / μl) and the detection time was shortened by 1 /2. The established assay could be used to detect PRV but could not be used to detect PCV2,PPV,CSFV or PRRSV. Various tissues were collected from Bama miniature pigs with latent PRV infection under sterile conditions for real-time PCR detection. Results showed that viral copy number in the brain,nasal swab,inguinal lymph node,liver,lung and spleen was above 20,while PRV was not detected in the kidney and heart tissues. [Conclusion]The established SYBR Green I real-time quantitative PCR assay for PRV LAT detection was specific,sensitive and rapid,which could be used for pathogen monitoring,epidemiological investigation and quantitative study of PRV. | Sufang LU Guangjun GUO Ling MO Feng WEI Bingmei DONG Wentong ZHANG Zhiqiang SHEN | 2015 | Agricultural Biotechnology2015，4，3： | 2 |
| 6 | Expression of Pseudorabies Virus gE Core Epitopes in Escherichia coli Strain BL21 and Utilization of Indirect PRV gE-ELISA显示文摘Pseudorabies virus glycoprotein E( PRV gE) has been recognized as a suitable diagnostic antigen for pseudorabies. In order to produce gE antigen in large quantities and at low cost,a gene fragment encoding PRV gE core epitopes was expressed in E. coli BL21 expression system. SDS-PAGE and Western Blotting revealed that the expression product in culture supernatant of E. coli BL21 was a recombinant protein,approximately 51. 8 Kd. At 5 h post-induction,protein concentration assay showed that the expression product amounted to 1. 65 mg /ml,accounting for 24. 17% of total proteins in the culture supernatant. An indirect PRV gE-ELISA was established by using the recombinant expression product as a coating antigen. Cross-reactivity assay showed that this antigen was PRV specific. In addition,the assay was consistently reproducible. Comparison of detection results of 240 serum samples between PRV gE-ELISA and a commercially available PRV diagnostic kit showed that there was no significant difference between these two methods( P > 0. 05). | Guangjun GUO Sufang LU Guanggang QU Feng LI Lin DONG Yanli BI Jinliang WANG Feng WEI Na TANG Chunling ZHANG Zhuang DING Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，4： | 2 |
| 7 | Photosensitive liquid crystalline supramolecules self assembled from ionic liquid crystal and polyelectrolyte for laser-induced optical anisotropy显示文摘 | Xiao Sufang Lu Xuemin Lu Qinghua | 2008 | Macromolecules2008，41，11： | 1 |
| 8 | Photosensitive polymer from ionic self-assembly of azobenzene dye and poly (ionic liquid) and its alignment characteristic toward liquid crystal molecules显示文摘 | Xiao Sufang Lu Xuemin Lu Qqinghua | 2007 | Macromolecules2007，40，22： | 1 |
| 9 | A photosensitive fluorinated ionic complex with tunable surface wetting properties : Mesostructure and photosensitivity显示文摘 | Lu Xuemin Xiao Sufang Chen Xumeng | 2011 | Polymer Chemistry2011，2，11： | 1 |
| 10 | Photoinduced orientation in ionic-bonding photo-cross-linkable polymers 显示文摘 | Kong Hui Lu Xuemin Xiao Sufang | 2009 | Polymer2009，50，5： | 1 |
| 11 | Segmentation for satellite cloud image by combining fractal features and continuous wavelet transform显示文摘 | Zhang Changjiang Lu Juan Shu Sufang | 2009 | Intelligent Systems and Applications2009，，： | 1 |
| 12 | Microwave absorption response of nickel/graphene nanocomposites prepared by electrodeposition显示文摘 | Jianjun Fang Wenke Zha Meng Kang Shenghui Lu Lei Cui Sufang Li | 2013 | Journal of Materials Science2013，，： | 1 |
| 13 | In vitro Transcriptome Analysis of Two Chinese Isolates of Streptococcus suis Serotype 2显示文摘The Streptococcus suis serotype 2(S. suis 2) isolates 05ZYH33 and 98HAH33 have caused severe human infections in China. Using a strand-specific RNA-seq analysis, we compared the in vitro transcriptomes of these two Chinese isolates with that of a reference strain(P1/7). In the89 K genomic island that is specific to these Chinese isolates, a toxin–antitoxin system showed relatively high levels of transcription among the S. suis. The known virulence factors with high transcriptional activity in these two highly-pathogenic strains are mainly involved in adhesion, biofilm formation, hemolysis and the synthesis and transport of the outer membrane protein. Furthermore,our analysis of novel transcripts identified over 50 protein-coding genes with one of them encoding a toxin protein. We also predicted over 30 small RNAs(s RNAs) in each strain, and most of them are involved in riboswitches. We found that six s RNA candidates that are related to bacterial virulence, including csp A and rli38, are specific to Chinese isolates. These results provide insight into the factors responsible for the difference in virulence among the different S. suis 2 isolates. | Dake Zhang Nan Du Sufang Ma Qingtao Hu Guangwen Lu Wei Chen Changqing Zeng | 2014 | Genomics, Proteomics & Bioinformatics2014，12，6： | 1 |
| 14 | Novel Aqueous Soluble Cobalt Phthalocyanine : Synthesis and Catalytic Activ- ity on Oxidation of 2-Mercaptoethanol显示文摘 | Pan Yong Chen Wenxing Lu Sufang | 2005 | Dyes Pigments''2005，66，2： | 1 |
| 15 | Fusion of EGFP and porcine α 1,3GT genes decrease GFP expression显示文摘Objective:To investigate the effect of fusion proteins expressed by the fused gene of porcineα1,3 galactosyltransierase(α1,3 GT) and enhanced green fluorescent protein(EGFP) on the green fluorescence intensity of EGFP.Methods:The fragment containingα1.3GT was firstly recovered after the pcDNA3.1-α1.3GT recombinant vector were digested with BamHl and EcoRI,and then,the resultant fragment was ligated to the pEGFP-N1 vector which was also digested with the same enzymes.The new recombinant eukaryotic expression pEGFP/a 1,3GT vector was obtained and sequenced.The pEGFP/α1,3GT was used to transfect human lung carcinoma cells A549 and HEKC 293FT,and the expression of EGFP was quantitatively analyzed by fluorescent microscope and flow cytometry.Results:The positive percentage of A549 was 80.5%,and that of 293 FT was 86.5%48 hours after the two cell lines both were transfected by pEGFP-N1.The positive percentage of A549 was 75.8%,and that of 293 FT was 81.2%48 hours after the two cell lines were transfected by pEGFP/α1.3GT.The mean fluorescence intensities of A549 transfected with pEGFP-N1 and pEGFP/α1.3GT were 1.21 and 0.956,respectively when compared with that of A549 without transfection.Meanwhile,the those of the 293FT that were transfected with pEGFP-N1 and pEGFP/αl,3GT were 7.66 and 1.00.respectively when compared with that of 293FT cells without transfection.Conclusions:These results suggested that the expression of EGFP gene fused with porcineα1,3GT gene was partly inhibited. | Yongxiang Zhao Jing Tang Qin Yao Yuan Zhou Huange Zhao Xiaoyun Zeng Jiaqi Shi Guorong Luo Xiaoxun Xie Sufang Zhou Zuguo Liu Xiaoling Lu Donghai Lin Jianming Liu | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，12： | 0 |
| 16 | Expression of Porcine Circovirus Type 2 ORF2 Gene in Myocardial Cells of Detached Cherry Valley Duck显示文摘The objective of this study was to construct the recombinant eukaryotic expression plasmid of ORF2 gene harboring enhanced green fluorescent protein(EGFP) report gene. ORF2 gene of porcine circovirus type2 cloned by PCR was ligated to the expression vector pEGFP-N1,which contains enhanced green fluorescent protein(EGFP) report gene,the recombinant eukaryotic expression plasmid pEGFP-N1-ORF2 was constructed successfully and was transfected into prepared duck myocardial cells(DMCs) by lipofectin. According to the result,the fluorescence expression was directly detected with fluorescence microscope,and the expression of ORF2 were analyzed by RT-PCR and indirect immunofluorescence assay(IFA) respectively. About 48 h after transfection,green fluorescent can be observed on transfected cells: T-PCR and IFA were positive. This indicated that ORF2 gene of PCV2 was expressed efficiently in transfected duck myocardial cells. | Wenxiu WANG Sufang LU Shijun FU Guangjun GUO Ling MO Yueqiang XIAO Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，5： | 0 |
| 17 | Differential regulation of JAK1 expression by ETS1 associated with predisposition to primary biliary cholangitis显示文摘Primary biliary cholangitis(PBC)is an autoimmune liver disease characterized by the destruction of intrahepatic small bile ducts and progressive cholestasis,eventually leading to liver cirrhosis and hepatic failure without appropriate treatment(Terziroli Beretta-Piccoli et al.,2019). | Peng Jiang Chan Wang Mingming Zhang Ye Tian Weifeng Zhao Junyi Xin Yexi Huang Zhibin Zhao Wenjuan Sun Jie Long Ruqi Tang Fang Qiu Xingjuan Shi Yi Zhao Li Zhu Na Dai Lei Liu Xudong Wu Jinshan Nie Bo Jiang Youlin Shao Yueqiu Gao Jianjiang Yu Zhigang Hu Zhidong Zang Yuhua Gong Yaping Dai Lan Wang Ningling Ding Ping Xu Sufang Chen Lu Wang Jing Xu Luyao Zhang Junyan Hong Ruonan Qian Hu Li Xuan Jiang Congwei Chen Wenyan Tian Jian Wu Yuzhang Jiang Chongxu Han Kui Zhang Hong Qiu Li Li Hong Fan Liming Chen Jianqiong Zhang Zhongsheng Sun Xiao Han Zhenhua Dai Erguang Li M.Eric Gershwin Zhexiong Lian Xiong Ma Michael F.Seldin Weichang Chen Meilin Wang Xiangdong Liu | 2023 | Journal of Genetics and Genomics2023，50，10： | 0 |
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