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| 1 | Loss of 5-hydroxymethylcytosine is linked to gene body hypermethylation in kidney cancer显示文摘5-methylcytosine (5mC ) 和它的氧化形式 5-hydroxymethylcytosine (5hmC ) 被建议了涉及 tumorigenesis。因为印射方法的广泛地使用的 5mC 的读出,定序的重亚硫酸盐(BS-seq ) ,是 5mC 的和, 5hmC 层次, 5mC/5hmC 模式和这二修正的关系仍然保持糟糕理解。由介绍真实 5mC (BS-seq 由帮助 Tet 的 BS-seq 改正了, TAB-seq ) 并且 5hmC (TAB-seq ) 在单个核苷酸的分辨率同时铺平,我们这里证明与匹配的正常纸巾相比没有在肾肿瘤的 5mC 的全球损失。相反地, 5hmC 全球性被失去在几乎所有肾肿瘤纸巾。在肿瘤纸巾的 5hmC 水平是为肾癌症的一个独立预示的标记,与与更短的全面幸存联系的 5hmC 的底层。而且,我们证明 5hmC 的损失与匹配的正常纸巾相比在肿瘤被连接到 hypermethylation,特别地在基因身体区域。惊人地,基因身体 hypermethylation 显著地与肿瘤相关的基因的 silencing 被联系。IDH1 的 Downregulation 作为在肾癌症位于 5hmC 损失下面的机制被识别。恢复 5hmC 层次稀释了肿瘤房间的侵略能力并且在一个异种皮移植模型压制了肿瘤生长。一起,我们的结果证明 5hmC 的损失在由改变 DNA methylation 模式的肾癌症是一个预示的标记和一个 oncogenic 事件。 | Ke Chen Jing Zhang Zhongqiang Guo Qin Ma Zhengzheng Xu Yuanyuan Zhou Ziying Xu Zhongwu Li Xiongjun- Ye Xuesong Li Bifeng Yuan Yuwen Ke Chuan He Liqun Zhou Jiang Liu WeiminCi | 2016 | Cell Research2016，26，1： | 10 |
| 2 | Jerk-limited feedrate scheduling and optimization for five-axis machining using new piecewise linear programming approach显示文摘In this paper, a new computation method and an optimization algorithm are presented for feedrate scheduling of five-axis machining in compliance with both machine drive limits and process limits. Five-axis machine tool with its ability of controlling tool orientation to follow the sculptured surface contour has been widely used in modern manufacturing industry. Feedrate scheduling serving as a kernel of CNC control system plays a critical role to ensure the required machining accuracy and reliability for five-axis machining. Due to the nonlinear coupling effects of all involved drive axes and the saturation limit of servo motors, the feedrate scheduling for multi-axis machining has long been recognized and remains as a critical challenge for achieving five-axis machine tools’ full capacity and advantage. To solve the nonlinearity nature of the five-axis feedrate scheduling problems, a relaxation mathematical process is presented for relaxing both the drive motors’ physical limitations and the kinematic constraints of five-axis tool motions. Based on the primary optimization variable of feedrate, the presented method analytically linearizes the machining-related constraints, in terms of the machines’ axis velocities, axis accelerations and axis jerks. The nonlinear multi-constrained feedrate scheduling problem is transformed into a manageable linear programming problem. An optimization algorithm is presented to find the optimal feedrate scheduling solution for the five-axis machining problems. Both computer implementation and laboratorial experiment testing by actual machine cutting were conducted and presented in this paper. The experiment results demonstrate that the proposed method can effectively generate efficient feedrate scheduling for five-axis machining with constraints of the machine tool physical constraints and limits. Compared with other existing numerical methods, the proposed method is able to find an accurate analytical solution for the nonlinear constrained five-axis feedrate scheduling problems without compromising the efficiency of the machining processes. | SUN YuWen CHEN ManSen JIA JinJie LEE Yuan-Shin GUO DongMing | 2019 | Science China(Technological Sciences)2019，62，7： | 7 |
| 3 | Analytical modeling of geometric errors induced by cutter runout and tool path optimization for five-axis flank machining显示文摘The cutter runout effect has significant influence on the shape of cutter swept surface and the machining surface quality. Hence,it is necessary to integrate the cutter runout effect in cutter swept surface modeling,geometric error prediction and tool path optimization for five-axis flank machining. In this paper,an envelope surface model considering cutter runout effect is first established,and geometric errors induced by runout effect are derived based on the relative motion analysis between the cutter and part in machining. In the model,the cutter runout is defined by four parameters,including inclination angle,location angle,offset value and the length of cutter axis. Then the runout parameters are integrated into the rotation surface of each cutting edge that is used to form the final cutter envelope surface for the five-axis machining process. Thus,the final resulting geometric errors of the machined surface induced by cutter runout can be obtained through computing the deviations from the nominal cutter swept surface. To reduce these errors,an iterative least square method is used to optimize the tool paths for five-axis flank machining. Finally,a validation example is given for a specific ruled surface. Results show the effectiveness and feasibility of the analytical model of geometric errors induced by cutter runout,and also show that the geometric errors can be reduced significantly using the proposed tool path planning method. | GUO Qiang SUN YuWen GUO DongMing | 2011 | Science China(Technological Sciences)2011，54，12： | 7 |
| 4 | 0.75 Gbit/s high-speed classical key distribution with mode-shift keying chaos synchronization of Fabry–Perot lasers显示文摘High-speed physical key distribution is diligently pursued for secure communication.In this paper,we propose and experimentally demonstrate a scheme of high-speed key distribution using mode-shift keying chaos synchronization between two multi-longitudinal-mode Fabry–Perot lasers commonly driven by a super-luminescent diode.Legitimate users dynamically select one of the longitudinal modes according to private control codes to achieve mode-shift keying chaos synchronization.The two remote chaotic light waveforms are quantized to generate two raw random bit streams,and then those bits corresponding to chaos synchronization are sifted as shared keys by comparing the control codes.In this method,the transition time,i.e.,the chaos synchronization recovery time is determined by the rising time of the control codes rather than the laser transition response time,so the key distribution rate is improved greatly.Our experiment achieved a 0.75-Gbit/s key distribution rate with a bit error rate of 3.8×10^(−3)over 160-km fiber transmission with dispersion compensation.The entropy rate of the laser chaos is evaluated as 16 Gbit/s,which determines the ultimate final key rate together with the key generation ratio.It is therefore believed that the method pays a way for Gbit/s physical key distribution. | Hua Gao Anbang Wang Longsheng Wang Zhiwei Jia Yuanyuan Guo Zhensen Gao Lianshan Yan Yuwen Qin Yuncai Wang | 2021 | Light(Science & Applications)2021，10，9： | 7 |
| 5 | Redesigned Surface Based Machining Strategy and Method in Peripheral Milling of Thin-walled Parts显示文摘Currently, simultaneously ensuring the machining accuracy and efficiency of thin-walled structures especially high performance parts still remains a challenge. Existing compensating methods are mainly focusing on 3-aixs machining, which sometimes only take one given point as the compensative point at each given cutter location. This paper presents a redesigned surface based machining strategy for peripheral milling of thin-walled parts. Based on an improved cutting force/heat model and finite element method(FEM) simulation environment, a deflection error prediction model, which takes sequence of cutter contact lines as compensation targets, is established. And an iterative algorithm is presented to determine feasible cutter axis positions. The final redesigned surface is subsequently generated by skinning all discrete cutter axis vectors after compensating by using the proposed algorithm. The proposed machining strategy incorporates the thermo-mechanical coupled effect in deflection prediction, and is also validated with flank milling experiment by using five-axis machine tool. At the same time, the deformation error is detected by using three-coordinate measuring machine. Error prediction values and experimental results indicate that they have a good consistency and the proposed approach is able to significantly reduce the dimension error under the same machining conditions compared with conventional methods. The proposed machining strategy has potential in high-efficiency precision machining of thin-walled parts. | JIA Zhenyuan GUO Qiang SUN Yuwen GUO Dongming | 2010 | Chinese Journal of Mechanical Engineering2010，23，3： | 6 |
| 6 | Compressional Wave Velocities in Some Volcanic Rocks at Pressure up to 4.5 GPa显示文摘INTRODUCTIONThecompressionalwavevelocity(vp)inrockmeasuredathighpressureandhightemperatureisofsignificantimpor-tancetoelucida... | Zhou Wenge Zhao Zhidan Xie Hongsen Li Yuwen Guo Jie Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002 Xu Ji ’an Institute of Earth Sciences, Academia Sinica, Nankang PO Box1 55, Taipei, Taiwan 115 | 1998 | Journal of Earth Science1998，17，2： | 3 |
| 7 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 8 | New Mathematical Method for the Determination of Cutter Runout Parameters in Flat-end Milling显示文摘The cutting force prediction is essential to optimize the process parameters of machining such as feed rate optimization, etc. Due to the significant influences of the runout effect on cutting force variation in milling process, it is necessary to incorporate the cutter runout parameters into the prediction model of cutting forces. However, the determination of cutter runout parameters is still a challenge task until now. In this paper, cutting process geometry models, such as uncut chip thickness and pitch angle, are established based on the true trajectory of the cutting edge considering the cutter runout effect. A new algorithm is then presented to compute the cutter runout parameters for flat-end mill utilizing the sampled data of cutting forces and derived process geometry parameters. Further, three-axis and five-axis milling experiments were conducted on a machining centre, and resulting cutting forces were sampled by a three-component dynamometer. After computing the corresponding cutter runout parameters, cutter forces are simulated embracing the cutter runout parameters obtained from the proposed algorithm. The predicted cutting forces show good agreements with the sampled data both in magnitude and shape, which validates the feasibility and effectivity of the proposed new algorithm of determining cutter runout parameters and the new way to accurately predict cutting forces. The proposed method for computing the cutter runout parameters provides the significant references for the cutting force prediction in the cutting process. | GUO Qiang SUN Yuwen GUO Dongming ZHANG Chuantai | 2012 | Chinese Journal of Mechanical Engineering2012，25，5： | 2 |
| 9 | Iso -parametric Tool Path Generation from Triangular Meshes for Free-form Surface Machining显示文摘 | Sun Yuwen Guo Dongming Jia Zhenyt an | 2006 | In- ternational Journal of Advanced Manufacturing Technology2006，28，7： | 1 |
| 10 | Estimationand experimental validation of cutting forces in ball-endmilling of sculptured surfaces 显示文摘 | SUN Yuwen REN Fei GUO Dongming | 2009 | International Journal ofMachine Tools and Manufacture2009，49，15： | 1 |
| 11 | A unified localization approach for machining allowance optimization of complex curved surfaces 显示文摘 | SUN Yuwen XU Jinting GUO Dongming | 2009 | Precision Engineering2009，33，4： | 1 |
| 12 | A Unified Localization Approach for Machining A1-lowance Optimization of Complex Curved Surfaces 显示文摘 | Sun Yuwen Xu Jinting Guo Dongming | 2009 | Precision Engineering2009，33，： | 1 |
| 13 | Estimation and experimental validation of cutting forces in ball-end milling of sculptured surfaces显示文摘 | Yuwen Sun Fei Ren Dongming Guo Zhenyuan Jia | 2009 | International Journal of Machine Tools and Manufacture2009，，15： | 1 |
| 14 | An automatic modeling strategy for the reverse engineering of wrap-around freeform surfaces显示文摘 | SUN Yuwen GUO Dongming JIA Zhenyuan | 2006 | International Journal of Materials and Product Technology2006，33，12： | 1 |
| 15 | Isopar ametric tool path generation from triangular meshes for free- form surface machining显示文摘 | SUN Yuwen GUO Dongming JIA Zhenyuan | 2006 | International Journal of Advaneed Manufacturing Technology2006，28，7： | 1 |
| 16 | A Unified Localization Approach for Machining Al- lowance Optimization of Complex Curved Surfaces 显示文摘 | Sun Yuwen Xu Jinting Guo Dongming | 2009 | Precision Engineering2009，33，4： | 1 |
| 17 | Guide Curve Based Interpolation Scheme of Parametric Curves for Precision CNC Machining显示文摘 | Sun Yuwen Wang Jun Guo Dongming | 2006 | International Journal of Machine Tools and Manufacture2006，46，34： | 1 |
| 18 | Iso-parametric tool path generation from triangular meshes for freeform surface machining 显示文摘 | Sun Yuwen Guo Dongming JIA zhenyuan | 2006 | International Journal of Advanced Manufacturing Technology2006，28，7： | 1 |
| 19 | Localization of Mobile Robot Aided for Large-Scale Construction Based on Optimized Artificial Landmark Map in Ongoing Scene显示文摘The effectiveness of mobile robot aided for architectural construction depends strongly on its accurate localization ability.Localization of mobile robot is increasingly important for the printing of buildings in the construction scene.Although many available studies on the localization have been conducted,only a few studies have addressed the more challenging problem of localization for mobile robot in large-scale ongoing and featureless scenes.To realize the accurate localization of mobile robot in designated stations,we build an artificial landmark map and propose a novel nonlinear optimization algorithm based on graphs to reduce the uncertainty of the whole map.Then,the performances of localization for mobile robot based on the original and optimized map are compared and evaluated.Finally,experimental results show that the average absolute localization errors that adopted the proposed algorithm is reduced by about 21%compared to that of the original map. | Zhen Xu Shuai Guo Tao Song Yuwen Li Lingdong Zeng | 2022 | Computer Modeling in Engineering & Sciences2022，，3： | 1 |
| 20 | Adaptive federate Interpolation with multiconstraints for five-axis parametric toolpath显示文摘 | Zhou Jianfeng Sun Yuwen Guo Dongming | 2014 | The International Journal of Advanced Manufacturing Technology2014，71，9： | 1 |
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