|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 2 | Jerk-limited feedrate scheduling and optimization for five-axis machining using new piecewise linear programming approach显示文摘In this paper, a new computation method and an optimization algorithm are presented for feedrate scheduling of five-axis machining in compliance with both machine drive limits and process limits. Five-axis machine tool with its ability of controlling tool orientation to follow the sculptured surface contour has been widely used in modern manufacturing industry. Feedrate scheduling serving as a kernel of CNC control system plays a critical role to ensure the required machining accuracy and reliability for five-axis machining. Due to the nonlinear coupling effects of all involved drive axes and the saturation limit of servo motors, the feedrate scheduling for multi-axis machining has long been recognized and remains as a critical challenge for achieving five-axis machine tools’ full capacity and advantage. To solve the nonlinearity nature of the five-axis feedrate scheduling problems, a relaxation mathematical process is presented for relaxing both the drive motors’ physical limitations and the kinematic constraints of five-axis tool motions. Based on the primary optimization variable of feedrate, the presented method analytically linearizes the machining-related constraints, in terms of the machines’ axis velocities, axis accelerations and axis jerks. The nonlinear multi-constrained feedrate scheduling problem is transformed into a manageable linear programming problem. An optimization algorithm is presented to find the optimal feedrate scheduling solution for the five-axis machining problems. Both computer implementation and laboratorial experiment testing by actual machine cutting were conducted and presented in this paper. The experiment results demonstrate that the proposed method can effectively generate efficient feedrate scheduling for five-axis machining with constraints of the machine tool physical constraints and limits. Compared with other existing numerical methods, the proposed method is able to find an accurate analytical solution for the nonlinear constrained five-axis feedrate scheduling problems without compromising the efficiency of the machining processes. | SUN YuWen CHEN ManSen JIA JinJie LEE Yuan-Shin GUO DongMing | 2019 | Science China(Technological Sciences)2019，62，7： | 7 |
| 3 | Analytical modeling of geometric errors induced by cutter runout and tool path optimization for five-axis flank machining显示文摘The cutter runout effect has significant influence on the shape of cutter swept surface and the machining surface quality. Hence,it is necessary to integrate the cutter runout effect in cutter swept surface modeling,geometric error prediction and tool path optimization for five-axis flank machining. In this paper,an envelope surface model considering cutter runout effect is first established,and geometric errors induced by runout effect are derived based on the relative motion analysis between the cutter and part in machining. In the model,the cutter runout is defined by four parameters,including inclination angle,location angle,offset value and the length of cutter axis. Then the runout parameters are integrated into the rotation surface of each cutting edge that is used to form the final cutter envelope surface for the five-axis machining process. Thus,the final resulting geometric errors of the machined surface induced by cutter runout can be obtained through computing the deviations from the nominal cutter swept surface. To reduce these errors,an iterative least square method is used to optimize the tool paths for five-axis flank machining. Finally,a validation example is given for a specific ruled surface. Results show the effectiveness and feasibility of the analytical model of geometric errors induced by cutter runout,and also show that the geometric errors can be reduced significantly using the proposed tool path planning method. | GUO Qiang SUN YuWen GUO DongMing | 2011 | Science China(Technological Sciences)2011，54，12： | 7 |
| 4 | Redesigned Surface Based Machining Strategy and Method in Peripheral Milling of Thin-walled Parts显示文摘Currently, simultaneously ensuring the machining accuracy and efficiency of thin-walled structures especially high performance parts still remains a challenge. Existing compensating methods are mainly focusing on 3-aixs machining, which sometimes only take one given point as the compensative point at each given cutter location. This paper presents a redesigned surface based machining strategy for peripheral milling of thin-walled parts. Based on an improved cutting force/heat model and finite element method(FEM) simulation environment, a deflection error prediction model, which takes sequence of cutter contact lines as compensation targets, is established. And an iterative algorithm is presented to determine feasible cutter axis positions. The final redesigned surface is subsequently generated by skinning all discrete cutter axis vectors after compensating by using the proposed algorithm. The proposed machining strategy incorporates the thermo-mechanical coupled effect in deflection prediction, and is also validated with flank milling experiment by using five-axis machine tool. At the same time, the deformation error is detected by using three-coordinate measuring machine. Error prediction values and experimental results indicate that they have a good consistency and the proposed approach is able to significantly reduce the dimension error under the same machining conditions compared with conventional methods. The proposed machining strategy has potential in high-efficiency precision machining of thin-walled parts. | JIA Zhenyuan GUO Qiang SUN Yuwen GUO Dongming | 2010 | Chinese Journal of Mechanical Engineering2010，23，3： | 6 |
| 5 | Genome wide abnormal DNA methylome of human blastocyst in assisted reproductive technology显示文摘Proper reprogramming of parental DNA methylomes is essential for mammalian embryonic development.However,it is unknown whether abnormal methylome reprogramming occurs and is associated with the failure of embryonic development.Here we analyzed the DNA methylomes of 57 blastocysts and 29 trophectoderm samples with different morphological grades during assisted reproductive technology(ART) practices.Our data reveal that the global methylation levels of high-quality blastocysts are similar(0.30 ± 0.02,mean ± SD).while the methylation levels of low-quality blastocysts are divergent and away from those of high-quality blastocysts.The proportion of blastocysts with a methylation level falling within the range of 0.30 ± 0.02 in different grades correlates with the live birth rate for that grade.Moreover,abnormal methylated regions are associated with the failure of embryonic development.Furthermore,we can use the methylation data of cells biopsied from trophectoderm to predict the blastocyst methylation level as well as to detect the aneuploidy of the blastocysts.Our data indicate that global abnormal methylome reprogramming often occurs in human embryos,and suggest that DNA methylome is a potential biomarker in blastocyst selection in ART. | Guoqiang Li Yang Yu Yong Fan Congru Li Xiaocui Xu Jialei Duan Rong Li Xiangjin Kang Xin Ma Xuepeng Chen Yuwen Ke Jie Yan Ying Lian Ping Liu Yue Zhao Hongcui Zhao Yaoyong Chen Xiaofang Sun Jianqiao Liu Jie Qiao Jiang Liu | 2017 | Journal of Genetics and Genomics2017，44，10： | 5 |
| 6 | Influence of MoSi_(2) on oxidation protective ability of TaB_(2)-SiC coating in oxygen-containing environments within a broad temperature range显示文摘TaB_(2)-SiC coating modified by different content of MoSi_(2) was fabricated on graphite substrate with SiC inner coating by liquid phase sintering to elevate the anti-oxidation capability of the TaB_(2)-SiC coatings.As compared to the sample with the TaB_(2)-40wt% SiC coating,the coating sample modified with MoSi_(2) exhibited a weight gain trend at lower temperatures,the fastest weight loss rate went down by 76%,and the relative oxygen permeability value reduced from about 1% to near 0.More importantly,the large amount of SiO_(2) glass phase produced over the coating during oxidation was in contact with the modification of MoSi_(2),which was proved to be beneficial to the dispersion of Ta-oxides.A concomitantly formed continuous Ta-Si-O-B compound glass layer showed excellent capacity to prevent oxygen penetration.However,when the TaB_(2) content was sacrificed to increase the MoSi_(2) content,the relative oxygen permeability of the coating increased instead of decreased.Thus,on the basis of ample TaB_(2) content,increasing the MoSi_(2) content of the coating is conducive to reducing the relative oxygen permeability of the coatings in a broad temperature region. | Xuanru REN Junshuai LV Wei LI Yuwen HU Ke SUN Can MA Hong’ao CHU Weiguang WANG Leihua XU Ziyu LI Peizhong FENG | 2020 | Journal of Advanced Ceramics2020，9，6： | 4 |
| 7 | Design and application of an Electric Tail Rotor Drive Control(ETRDC) for helicopters with performance tests显示文摘With the development of electric helicopters' motor technology and the widespread use of electric drive rotors, more aircraft use electric rotors to provide thrust and directional control.For a helicopter tail rotor, the wake of the main rotor influences the tail rotor's inflow and wake.In the procedure of controlling, crosswind will also cause changes to the tail disk load. This paper describes requirements and design principles of an electric motor drive and variable pitch tail rotor system. A particular spoke-type architecture of the motor is designed, and the performance of blades is analyzed by the CFD method. The demand for simplicity of moving parts and strict constraints on the weight of a helicopter makes the design of electrical and mechanical components challenging. Different solutions have been investigated to propose an effective alternative to the mechanical actuation system. A test platform is constructed which can collect the dynamic response of the thrust control. The enhancement of the response speed due to an individual motor speed control and variable-pitch system is validated. | Yuwen ZHANG Chen JIANG Yunjie WANG Fan SUN Haowen WANG | 2018 | Chinese Journal of Aeronautics2018，31，9： | 3 |
| 8 | Path,feedrate and trajectory planning for free-form surface machining:A state-of-the-art review显示文摘Nowadays,free-form surfaces have been widely used in various industrial fields.They are usually machined by CNC machine tools,but recently have also begun to be manufactured by industrial robotic arm manipulators,thanks to low cost,large operation reaching space and high machining flexibility of robot.So far,various methodologies have been proposed to improve efficiency and quality of free-form surface machining,thus this paper aims at providing a state-ofthe-art review on research advances in free-form surface machining.In this review,tool path generation,feedrate scheduling in Cartesian space and trajectory planning in joint space are focused for both CNC machining and robot machining,and their research statues,existing difficulties and key issues are discussed in detail.Finally,the feasible routes,breakthrough points and future development trend are also expounded. | Yuwen SUN Jinjie JIA Jinting XU Mansen CHEN Jinbo NIU | 2022 | Chinese Journal of Aeronautics2022，35，8： | 3 |
| 9 | A multi-order method for predicting stability of a multi-delay milling system considering helix angle and run-out effects显示文摘In this paper, a multi-delay milling system considering helix angle and run-out effects is firstly established. An exponential cutting force model is used to model the interaction between a work-piece and a cutting tool, and a new approach is presented for accurately calibrating exponential cutting force coefficients and cutter run-out parameters. Furthermore, based on an implicit multi-step Adams formula and an improved precise time-integration algorithm, a novel stability prediction method is proposed to predict the stability of the system. The involved time delay term and periodic coefficient term are integrated as a comprehensive state term in the integral response which is approximated by the Adams formula. Then, a Floquet transition matrix with an arbitraryorder form is constructed by using a series of matrix multiplication, and the stability of the system is determined by the Floquet theory. Compared to classical semi-discretization methods and fulldiscretization methods, the developed method shows a good performance in convergence, efficiency,accuracy, and multi-order complexity. A series of cutting tests is further carried out to validate the practicability and effectiveness of the proposed method. The results show that the calibration process needs a time of less than 5 min, and the stability prediction method is effective. | Shanglei JIANG Yuwen SUN | 2018 | Chinese Journal of Aeronautics2018，31，6： | 3 |
| 10 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 11 | New Mathematical Method for the Determination of Cutter Runout Parameters in Flat-end Milling显示文摘The cutting force prediction is essential to optimize the process parameters of machining such as feed rate optimization, etc. Due to the significant influences of the runout effect on cutting force variation in milling process, it is necessary to incorporate the cutter runout parameters into the prediction model of cutting forces. However, the determination of cutter runout parameters is still a challenge task until now. In this paper, cutting process geometry models, such as uncut chip thickness and pitch angle, are established based on the true trajectory of the cutting edge considering the cutter runout effect. A new algorithm is then presented to compute the cutter runout parameters for flat-end mill utilizing the sampled data of cutting forces and derived process geometry parameters. Further, three-axis and five-axis milling experiments were conducted on a machining centre, and resulting cutting forces were sampled by a three-component dynamometer. After computing the corresponding cutter runout parameters, cutter forces are simulated embracing the cutter runout parameters obtained from the proposed algorithm. The predicted cutting forces show good agreements with the sampled data both in magnitude and shape, which validates the feasibility and effectivity of the proposed new algorithm of determining cutter runout parameters and the new way to accurately predict cutting forces. The proposed method for computing the cutter runout parameters provides the significant references for the cutting force prediction in the cutting process. | GUO Qiang SUN Yuwen GUO Dongming ZHANG Chuantai | 2012 | Chinese Journal of Mechanical Engineering2012，25，5： | 2 |
| 12 | Iso -parametric Tool Path Generation from Triangular Meshes for Free-form Surface Machining显示文摘 | Sun Yuwen Guo Dongming Jia Zhenyt an | 2006 | In- ternational Journal of Advanced Manufacturing Technology2006，28，7： | 1 |
| 13 | Estimationand experimental validation of cutting forces in ball-endmilling of sculptured surfaces 显示文摘 | SUN Yuwen REN Fei GUO Dongming | 2009 | International Journal ofMachine Tools and Manufacture2009，49，15： | 1 |
| 14 | A unified localization approach for machining allowance optimization of complex curved surfaces 显示文摘 | SUN Yuwen XU Jinting GUO Dongming | 2009 | Precision Engineering2009，33，4： | 1 |
| 15 | A Unified Localization Approach for Machining A1-lowance Optimization of Complex Curved Surfaces 显示文摘 | Sun Yuwen Xu Jinting Guo Dongming | 2009 | Precision Engineering2009，33，： | 1 |
| 16 | The application of fuzzy com- prehensive evaluation model in the teaching evaluation sys- tem显示文摘 | SUN Shuwen ZHAI Yuwen | 2013 | Applied mechanics and materials2013，30，3306： | 1 |
| 17 | Anti-inflammatory activities of aqueous extract from Radix Ophiopogon japonicus and its two constituents 显示文摘 | Kou Junping Sun Yang Lin Yuwen | 2005 | Biol Pharm Bull2005，28，7： | 1 |
| 18 | Estimation and experimental validation of cutting forces in ball-end milling of sculptured surfaces显示文摘 | Yuwen Sun Fei Ren Dongming Guo Zhenyuan Jia | 2009 | International Journal of Machine Tools and Manufacture2009，，15： | 1 |
| 19 | Effect of thinning intensity on understory herbaceous diversity and biomass in mixed coniferous and broad-leaved forests of Changbai Mountain显示文摘Background:Herbs are an important part of the forest ecosystem,and their diversity and biomass can reflect the restoration of vegetation after forest thinning disturbances.Based on the near-mature secondary coniferous and broad-leaved mixed forest in Jilin Province Forestry Experimental Zone,this study analyzed seasonal changes of species diversity and biomass of the understory herb layer after different intensities of thinning.Results:The results showed that although the composition of herbaceous species and the ranking of importance values were affected by thinning intensity,they were mainly determined by seasonal changes.Across the entire growing season,the species with the highest importance values in thinning treatments included Carex pilosa,Aegopodium alpestre,Meehania urticifolia,and Filipendula palmata,which dominated the herb layer of the coniferous and broad-leaved mixed forest.The number of species,Margalef index,Shannon-Wiener index and Simpson index all had their highest values in May,and gradually decreased with months.Pielou index was roughly inverted“N”throughout the growing season.Thinning did not increase the species diversity.Thinning can promote the total biomass,above-and below-ground biomass.The number of plants per unit area and coverage were related to the total biomass,above-and below-ground biomass.The average height had a significantly positive correlation with herb biomass in May but not in July.However,it exerted a significantly negative correlation with herb biomass in September.The biomass in the same month increased with increasing thinning intensity.Total herb biomass,above-and below-ground biomass showed positive correlations with Shannon-Winner index,Simpson index and Pielou evenness index in May.Conclusions:Thinning mainly changed the light environment in the forest,which would improve the plant diversity and biomass of herb layer in a short time.And different thinning intensity had different effects on the diversity of understory herb layer.The findings provide theoretical basis and reference for reasonable thinning and tending in coniferous and broad-leaved mixed forests. | Gerong Wang Yue Sun Mo Zhou Naiqian Guan Yuwen Wang Runhua Jiang Zhiyu Liu Mengjia Wu Fucai Xia | 2021 | Forest Ecosystems2021，8，4： | 1 |
| 20 | Total colorings of planar graph without adjacent triangles显示文摘 | Sun Xiangyong Wu Jianliang Wu Yuwen | | 0，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员