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| 1 | Nucleocytoplasmic Trafficking of the Arabidopsis WD40 Repeat Protein XIW1 Regulates ABI5 Stability and Abscisic Acid Responses显示文摘WD40 repeat-containing proteins(WD40 proteins)serve as versatile scaffolds for protein-protein interac-tions,modulating a variety of cellular processes such as plant stress and hormone responses.Here we report the identification of a WD40 protein,XIW1(for XPO1-interacting WD40 protein 1),which positively regulates the abscisic acid(ABA)response in Arabidopsis.XIW1 is located in the cytoplasm and nucleus.We found that it interacts with the nuclear transport receptor XPO1 and is exported by XPO1 from the nucleus.Mutation of XIW1 reduces the induction of ABA-responsive genes and the accumulation of ABA Insensitive 5(ABI5),causing mutant plants with ABA-insensitive phenotypes during seed germination and seedling growth,and decreased drought stress resistance.ABA treatment upregulates the expression of XIW1,and both ABA and abiotic stresses promote XIW1 accumulation in the nucleus,where it interacts with ABI5.Loss of XIW1 function results in rapid proteasomal degradation of ABI5.Taken together,these findings suggest that XIW1 is a nucleocytoplasmic shuttling protein and plays a positive role in ABA responses by interacting with and maintaining the stability of ABI5 in the nucleus. | Xuezhong XU Wang Wan Guobin Jiang Yue Xi Haijian Huang Jiajia Cai Yanan Chang Cheng-Guo Duan Satendra KMangrauthia Xinxiang Peng Jian-Kang Zhu Guohui Zhu | 2019 | Molecular Plant2019，12，12： | 8 |
| 2 | Comparison of attitude determination approaches using multiple Global Positioning System (GPS) antennas显示文摘GPS-based attitude system is an important research field, since it is a valuable technique for the attitude determination of platforms. There exist two classes approaches for attitude determination using the GPS. The one determines attitude via baseline estimates in two frames, the other one solves for attitude by incorporating the attitude parameters directly into the GPS measurements. However, comparisons between these two classes approaches have been unexplored. First of all, two algorithms are introduced in detail which on behalf of these two kinds of approaches. Then we present numerical simulations demonstrating the performance of our algorithms and provide a comparison evaluating. | Wang Bing Sui Lifen Xiao Guorui Duan Yu Qi Guobin | 2013 | Geodesy and Geodynamics2013，4，1： | 2 |
| 3 | Gas accumulation conditions and key technologies for exploration & development of Sinian and Cambrian gas reservoirs in Anyue gasfield显示文摘Exploration of the Sinian-Lower Paleozoic formations in the Sichuan Basin starts from the 1950s,and is tortuous and complex.To summarize the exploration experiences is of great significance of reference for exploration of oil and gas in deep carbonate rocks of the Sichuan Basin.Gas accumulation in the Anyue oversize gasfield is chiefly controlled by the Deyang-Anyue intra-platform rift and central Sichuan paleouplift.The intra-platform rift controls the scale hydrocarbon generation center and effective hydrocarbon plays.The Anyue gasfield is characterized by near-source hydrocarbon accumulation.The marginal zone of intra-platform rift and high part of the paleouplift control the scale distribution of favorable facies belts of reservoir development,and overlapped by paleokarstic reformation,the reservoirs are widely distributed.The intra-platform rift and paleouplift jointly control formation of the scale traps and hydrocarbon enrichment.Gas reservoir features and major controlling factors for hydrocarbon accumulation have some differences in the main play strata,such as Longwangmiao Formation,and Member 4 and Member 2 of Dengying Formation.The gas reservoir in the Longwangmiao Formation is a lithologic gas reservoir under the tectonic setting,and the inherited paleouplift is a key for reservoir formation and hydrocarbon accumulation.The gas reservoir in the Member 4 of Dengying Formation is mainly controlled by large structural-stratigraphic composite traps,and the gas reservoir in the Member 2 of Dengying Formation is the structural trap.Distribution of the large gasfields in the Dengying Formation is controlled by the intra-platform rift,near-source hydrocarbon-reservoir configuration controlled by the ancient erosion plane and stratigraphic sealing in the updip direction.For exploration and development of the Anyue gasfield,four series of technologies are developed,namely,reservoir seismic fine description technology,well logging fine evaluation technology of gas layers,fast drilling technology of complex strata,and stimulation technology for high temperature and high pressure gas layers.Technical progresses provide powerful guarantee for efficient exploration and development of the oversize gasfields. | Yueming Yang Yu Yang Guang Yang Jiarong Song LongWen Chuanguang Deng Maolong Xia Qi Ran Guobin Duan Bing Luo Bing Xie | 2018 | Petroleum Research2018，3，3： | 1 |
| 4 | Rapid Screening of Nine Illicit Drugs in Human Blood and Urine by Direct Analysis in Real‑Time Mass Spectrometry显示文摘We aimed to establish for the rapid detection of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in human blood and urine by direct analysis in real‑time coupled with tandem mass spectrometry(DART‑MS/MS).These samples were extracted by acetonitrile‑methanol(V/V=4:1),using DART 12 Dip‑it automatic sampling system.They were injected at 400℃,and analyzed by positive ion and multiple reaction monitoring mode.The detection limits of morphine,O6‑Monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin were 100,50,50,100,20,20,10,1,and 0.01 ng/mL,respectively.The practical cases contained methamphetamine,codeine,cocaine,and O6‑monoacetylmorphine were detected accurately and rapidly.The method has the advantages of high sensitivity and good accuracy.The sample processing is simple and can be analyzed in a short time.This method is suitable for the analysis of morphine,O6‑monoacetylmorphine,heroin,codeine,cocaine,methamphetamine,ketamine,methadone,and dolantin in some practical cases. | Ying Zhang Wenfang Zhang Guobin Xin Li Liu Xiaokun Duan Charles Liu | 2019 | Journal of Forensic Science and Medicine2019，5，3： | 0 |
| 5 | A comparative study on the genomes,transcriptomes,and metabolic properties of Escherichia coli strains Nissle 1917,BL21(DE3),and MG1655显示文摘Escherichia coli is the most well-studied model prokaryote and has become an indispensable host for the biotech-nological production of proteins and biochemicals.In particular,the probiotic status of one E.coli strain,E.coli Nissle 1917(EcN)has helped it become a new favorite amongst synthetic biologists.To broaden its potential applications,here we assemble a comparative study on the genomes,transcriptomes,and metabolic properties of E.coli strains EcN,BL21(DE3),and MG1655.Comparative genomics data suggests that EcN possesses 1404 unique CDSs.In particular,EcN has additional iron transport systems which endow EcN with a higher tolerance to iron scarcity when compared to two other E.coli strains.EcN transcriptome data demonstrates that E.coli strains EcN,BL21(DE3),and MG1655 all have comparable activities of the central metabolic pathway,however only EcN inherits the arginine deiminase pathway.Additionally,we found that EcN displayed a lower expres-sion of ribosomal proteins compared to BL21(DE3)and MG1655.This comparative study on E.coli strains EcN,BL21(DE3),and MG1655 aims to provide a reference for further engineering EcN as a biotechnological tool. | Linlin Zhao Guobin Yin Yonglin Zhang Chaofan Duan Yang Wang Zhen Kang | 2022 | Engineering Microbiology2022，2，1： | 0 |
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