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| 1 | H7N9 virulent mutants detected in chickens in China pose an increased threat to humans显示文摘 | Jianzhong Shi Guohua Deng Huihui Kong Chunyang Gu Shujie Ma Xin Yin Xianying Zeng Pengfei Cui Yan Chen Huanliang Yang Xiaopeng Wan Xiurong Wang Liling Liu Pucheng Chen Yongping Jiang Jinxiong Liu Yuntao Guan Yasuo Suzuki Mei Li Zhiyuan Qu Lizheng Guan Jinkai Zang Wenli Gu Shuyu Han Yangming Song Yuzhen Hu Zeng Wang Linlin Gu Wenyu Yang Libin Liang Hongmei Bao Guobin Tian Yanbing Li Chuanling Qiao Li Jiang Chengjun Li Zhigao Bu Hualan Chen | 2017 | Cell Research2017，27，12： | 70 |
| 2 | Luminescence and energy transfer processes in Lu 2 SiO 5 :Ce 3+ scintillator显示文摘 | Bo Liu Chaoshu Shi Min Yin Yibin Fu Guobin Zhang Guohao Ren | 2005 | Journal of Luminescence2005，，2： | 1 |
| 3 | Downregulation of the miR-30 family microRNAs contributes to endoplasmic reticulum stress in cardiac muscle and vascular smooth muscle cells显示文摘 | Ming Chen Guiling Ma Yin Yue Yu Wei Qiang Li Zichuan Tong Lin Zhang Guobin Miao Jianjun Zhang | 2014 | International Journal of Cardiology2014，，： | 1 |
| 4 | 2017年中国出现的H7N9高致病性病毒的快速进化显示文摘文章简介为了防控H7N9病毒,中国2017年9月开始对鸡进行了H5/H7二价禽流感灭活疫苗。为了解H7N9病毒在中国家禽中的分布情况,研究团队进行了系统的禽流感病毒监测。2017年2月至2017年5月之间在379个活禽市场和665个家禽养殖场共采集30201份样品(其中活禽市场采集12504份. | Jianzhong Shi Guohua Deng Shujie Ma Xianying Zeng Xin Yin Mei Li Bo Zhang Pengfei Cui Yan Chen Huanliang Yang Xiaopeng Wan Liling Liu Pucheng Chen Yongping Jiang Yuntao Guan Jinxiong Liu Wenli Gu Shuyu Han Yangming Song Libin Liang Zhiyuan Qu Yujie Hou Xiurong Wang Hongmei Bao Guobin Tian Yanbing Li Li Jiang Chengjun Li 陈化兰 | 2019 | 科学新闻2019，0，2： | 1 |
| 5 | HMGB1 recruits myeloid derived suppressor cells to promote peritoneal dissemination of colon cancer after resection显示文摘 | Wei Li Ke Wu Ende Zhao Liang Shi Ruidong Li Peng Zhang Yuping Yin Xiaoming Shuai Guobin Wang Kaixiong Tao | 2013 | Biochemical and Biophysical Research Communicatio2013，，： | 1 |
| 6 | A comparative study on the genomes,transcriptomes,and metabolic properties of Escherichia coli strains Nissle 1917,BL21(DE3),and MG1655显示文摘Escherichia coli is the most well-studied model prokaryote and has become an indispensable host for the biotech-nological production of proteins and biochemicals.In particular,the probiotic status of one E.coli strain,E.coli Nissle 1917(EcN)has helped it become a new favorite amongst synthetic biologists.To broaden its potential applications,here we assemble a comparative study on the genomes,transcriptomes,and metabolic properties of E.coli strains EcN,BL21(DE3),and MG1655.Comparative genomics data suggests that EcN possesses 1404 unique CDSs.In particular,EcN has additional iron transport systems which endow EcN with a higher tolerance to iron scarcity when compared to two other E.coli strains.EcN transcriptome data demonstrates that E.coli strains EcN,BL21(DE3),and MG1655 all have comparable activities of the central metabolic pathway,however only EcN inherits the arginine deiminase pathway.Additionally,we found that EcN displayed a lower expres-sion of ribosomal proteins compared to BL21(DE3)and MG1655.This comparative study on E.coli strains EcN,BL21(DE3),and MG1655 aims to provide a reference for further engineering EcN as a biotechnological tool. | Linlin Zhao Guobin Yin Yonglin Zhang Chaofan Duan Yang Wang Zhen Kang | 2022 | Engineering Microbiology2022，2，1： | 0 |
| 7 | Design of artificial small regulatory trans-RNA for gene knockdown in Bacillus subtilis显示文摘Bacillus subtilis as the Gram-positive model bacterium has been widely used in synthetic biology and biotechnology while the regulatory RNA tools for B.subtilis are still not fully explored.Here,a bottom-up approach is proposed for designing artificial trans-acting sRNAs.By engineering the intrinsic sRNA SR6,a minimized core scaffold structure consisting of an 8 bp stem,a 4 nt loop,and a 9 nt polyU tail was generated and proven to be sufficient for constructing sRNAs with strong repression activity(83%).Moreover,we demonstrate this artificial sRNA system functions well in an hfq-independent manner and also achieves strong repression efficiency in Escherichia coli(above 80%).A structure-based sRNA design principle was further developed for the automatic generation of custom sRNAs with this core scaffold but various sequences,which facilitates the manipulation and avoids structure disruption when fusing any base-pairing sequence.By applying these auto-designed sRNAs,we rapidly modified the cell morphology and biofilm formation,and regulated metabolic flux toward acetoin biosynthesis.This sRNA system with cross-species regulatory activities not only enriched the gene regulation toolkit in synthetic biology for B.subtilis and E.coli but also enhanced our understanding of trans-acting sRNAs. | Guobin Yin Anqi Peng Luyao Zhang Yang Wang Guocheng Du Jian Chen Zhen Kang | 2023 | Synthetic and Systems Biotechnology2023，8，1： | 0 |
| 8 | Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities显示文摘Gene regulation by trans-acting small RNAs(sRNAs)has considerable advantages over other gene regulation strategies.However,synthetic sRNAs mainly take natural sRNAs(MicC or SgrS)as backbones and comprise three functional elements folding into two or more stem-loop structures:an mRNA base pairing region,an Hfq-binding structure,and a rho-independent terminator.Due to limited numbers of natural sRNAs and complicated backbone structures,synthetic sRNAs suffer from low activity programmability and poor structural modularity.Moreover,natural sRNA backbone sequences may increase the possibility of unwanted recombination.Here,we present a bottom-up approach for creating structure defined single-stem loop small non-coding RNAs(ssl-sRNAs),which contain a standardized scaffold of a 7 bp-stem-4 nt-loop-polyU-tail and a 24 nt basing pairing region covering the first eight codons.Particularly,ssl-sRNA requires no independent Hfq-binding structure,as the polyU tail fulfills the roles of binding Hfq.A thermodynamic-based scoring model and a web server sslRNAD(http://gffzz5b632577293c4c46hn60bk9qfcf906f0x.ffgz.tsg.suse.edu.cn/)were developed for automated design of ssl-sRNAs with well-defined structures and programmable activities.ssl-sRNAs displayed weak polar effects when regulating polycistronic mRNAs.The ssl-sRNA designed by sslRNAD showed regulatory activities in both Escherichia coli and Bacillus subtilis.A streamlined workflow was developed for the construction of customized ssl-sRNA and ssl-sRNA libraries.As examples,the E.coli cell morphology was easily modified and new target genes of ergothioneine biosynthesis were quickly identified with ssl-sRNAs.ssl-sRNA and its designer sslRNAD enable researchers to rapidly design sRNAs for knocking down target genes. | Yang Wang Guobin Yin Huanjiao Weng Luyao Zhang Guocheng Du Jian Chen Zhen Kang | 2023 | Synthetic and Systems Biotechnology2023，8，1： | 0 |
| 9 | Dissipative Supramolecular Polymerization Powered by Light显示文摘A new method of light-powered dissipative supra-molecular polymerization is established,in which supramolecular polymerization is implemented in the far-from-equilibrium state.A bifunctional mono-mer containing two viologen moieties was designed.Upon inputting energy by light,the sys-tem was driven far from equilibrium,and the mono-mers were photoreduced and activated to form supramolecular polymers driven by 2∶1 host–guest complexation of the viologen cation radical and cucurbit[8]uril.As the system returned to equilibri-um,the supramolecular polymers depolymerized spontaneously by air oxidation.This method works in both linear and in cross-linked supramolecular polymerization.The strategy of light-powered dis-sipative supramolecular polymerization is anticipat-ed to have potential in the fabrication of functional supramolecular materials,especially in creating novel“living”materials. | Zihe Yin Guobin Song Yang Jiao Peng Zheng Jiang-Fei Xu Xi Zhang | 2019 | CCS Chemistry2019，1，4： | 0 |
| 10 | Supramolecular Polymerization Powered by Escherichia coli:Fabricating a Near-Infrared Photothermal Antibacterial Agent in Situ显示文摘An Escherichia coli reduction-powered supramolecular polymerization is reported,leading to the fabrication of a near-infrared(NIR)photothermal antibacterial agent in situ.To this end,a bifunctional monomer containing two viologen moieties was designed.WhenincubatingE.coli with thebifunctional monomer and cucurbit[8]uril,viologen moieties were reduced to viologen cation radicals by E.coli,and a supramolecular polymer with supramolecular dimers of viologen cation radicals integrated into the main chain was fabricated on the surface of E.coli. | Zihe Yin Yuchong Yang Jinpeng Yang Guobin Song Hao Hu Peng Zheng Jiang-Fei Xu | 2022 | CCS Chemistry2022，4，10： | 0 |
| 11 | Ratiometric fluorescent Si-FITC nanoprobe for immunoassay of SARS-CoV-2 nucleocapsid protein显示文摘Coronavirus disease 2019(COVID-19)highlights the importance of rapid and reliable diagnostic assays for the management of virus transmission.Here,we developed a one-pot hydrothermal method to prepare Si-FITC nanoparticles(NPs)for the fluorescent immunoassay of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)nucleocapsid protein(N protein).The synthesis of Si-FITC NPs did not need post-modification,which addressed the issue of quantum yield reduction during the coupling reaction.Si-FITC NPs showed two distinct peaks,Si fluorescence atλem=385 nm and FITC fluorescence atλem=490 nm.In the presence of KMnO_(4),Si fluorescence was decreased and FITC fluorescence was enhanced.Briefly,in the presence of N protein,catalase(CAT)-linked secondary antibody/reporter antibody/N protein/capture antibody immunocomplexes were formed on microplates.Subsequently,hydrogen peroxide(H_(2)O_(2))and Si-FITC NPs/KMnO_(4)were injected into the microplate together.The decomposition of H_(2)O_(2)by CAT resulted in remaining of KMnO_(4),which changed the fluorescence intensity ratio of Si-FITC NPs.The fluorescence intensity ratio correlated significantly with the N protein concentration ranging from 0.02 to 50.00 ng/mL,and the detection limit was 0.003 ng/mL,which was more sensitive than the commercial ELISA kit with a detection limit of 0.057 ng/mL.The N protein concentration can be accurately determined in human serum.Furthermore,the COVID-19 and non-COVID-19 patients were distinguishable by this method.Therefore,the ratiometric fluorescent immunoassay can be used for SARS-CoV-2 infection diagnosis with a high sensitivity and selectivity. | Guobin Mao Silu Ye Wen Yin Yang Yang Xinghu Ji Jin He Yingxia Liu Junbiao Dai Zhike He Yingxin Ma | 2023 | Nano Research2023，16，2： | 0 |
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