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| 1 | Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen-use efficiency in the wheat cultivar Kenong 9204显示文摘Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait. | Xiaoli Shi Fa Cui Xinyin Han Yilin He Long Zhao Na Zhang Hao Zhang Haidong Zhu Zhexin Liu Bin Ma Shusong Zheng Wei Zhang Jiajia Liu Xiaoli Fan Yaoqi Si Shuiquan Tian Jianqing Niu Huilan Wu Xuemei Liu Zhuo Chen Deyuan Meng Xiaoyan Wang Liqiang Song Lijing Sun Jie Han Hui Zhao Jun Ji Zhiguo Wang Xiaoyu He Ruilin Li Xuebin Chi Chengzhi Liang Beifang Niu Jun Xiao Junming Li Hong-Qing Ling | 2022 | Molecular Plant2022，15，9： | 6 |
| 2 | Stalagmite-inferred Holocene precipitation in northern Guizhou Province, China, and asynchronous termination of the Climatic Optimum in the Asian monsoon territory显示文摘An absolute-dated, bi-decadal-resolution, stalagmite oxygen-isotopic time series from Shigao Cave reveals the evolution of summer monsoon precipitation over the past 9.9 ka BP in northern Guizhou Province, Southwest China. The 18O-inferred climate conditions are divisible into three distinct stages: (1) a maximum humid era from 9.9-6.6 ka BP; (2) a gradual declining precipitation interval between 6.6-1.6 ka BP; and (3) a relatively low precipitation time window after 1.6 ka BP. Consistency of contemporaneous stalagmite Holocene 18O records between Shigao and other caves in the Indian and East Asian monsoon realms support the effect of primary orbital solar forcings on monsoonal precipitation. However, statistical analysis shows a significant spatial asynchroneity of the Holocene Optimum termination in the Asian monsoon territory. The Holocene Optimum ended at 7.2-7.4 ka BP in Oman, located in the Indian monsoon region, and at 5.6-5.8 ka BP in Central China, in the East Asian monsoon zone. In Southwest China, the termination occurred between these periods, at 6.6-7.0 ka BP, and was influenced by both monsoon systems. We propose that this spatially asynchronous ending of Holocene Optimum in Asia may be attributed to sea surface temperature changes in the western tropical Pacific, which is a primary moisture source for the East Asian monsoon. | JIANG XiuYang HE YaoQi SHEN ChuanChou KONG XingGong LI ZhiZhong CHANG YuWei | 2012 | Chinese Science Bulletin2012，57，7： | 6 |
| 3 | Visual Object Tracking via Cascaded RPN Fusion and Coordinate Attention显示文摘Recently,Siamese-based trackers have achieved excellent performance in object tracking.However,the high speed and deformation of objects in the movement process make tracking difficult.Therefore,we have incorporated cascaded region-proposal-network(RPN)fusion and coordinate attention into Siamese trackers.The proposed network framework consists of three parts:a feature-extraction sub-network,coordinate attention block,and cascaded RPN block.We exploit the coordinate attention block,which can embed location information into channel attention,to establish long-term spatial location dependence while maintaining channel associations.Thus,the features of different layers are enhanced by the coordinate attention block.We then send these features separately into the cascaded RPN for classification and regression.According to the two classification and regression results,the final position of the target is obtained.To verify the effectiveness of the proposed method,we conducted comprehensive experiments on the OTB100,VOT2016,UAV123,and GOT-10k datasets.Compared with other state-of-the-art trackers,the proposed tracker achieved good performance and can run at real-time speed. | Jianming Zhang Kai Wang Yaoqi He Lidan Kuang | 2022 | Computer Modeling in Engineering & Sciences2022，，9： | 0 |
| 4 | MSC based gene delivery methods and strategies improve the therapeutic efficacy of neurological diseases显示文摘Mesenchymal stem cells(MSCs)are promising seed cells for neural regeneration therapy owing to their plasticity and accessibility.They possess several inherent characteristics advantageous for the transplantation-based treatment of neurological disorders,including neural differentiation,immunosuppression,neurotrophy,and safety.However,the therapeutic efficacy of MSCs alone remains unsatisfactory in most cases.To improve some of their abilities,many studies have employed genetic engineering to transfer key genes into MSCs.Both viral and nonviral methods can be used to overexpress therapeutic proteins that complement the inherent properties.However,to date,different modes of gene transfer have specific drawbacks and advantages.In addition,MSCs can be functionalized through targeted gene modification to facilitate neural repair by promoting neural differentiation,enhancing neurotrophic and neuroprotective functions,and increasing survival and homing abilities.The methods of gene transfer and selection of delivered genes still need to be optimized for improved therapeutic and targeting efficacies while minimizing the loss of MSC function.In this review,we focus on gene transport technologies for engineering MSCs and the application of strategies for selecting optimal delivery genes.Further,we describe the prospects and challenges of their application in animal models of different neurological lesions to broaden treatment alternatives for neurological diseases. | Heng Zhou Yan He Wei Xiong Shuili Jing Xingxiang Duan Zhiyun Huang Gurek SNahal Youjian Peng Mingchang Li Yaoqi Zhu Qingsong Ye | 2023 | Bioactive Materials2023，，5： | 0 |
| 5 | Fine mapping and characterization of the awn inhibitor B1 locus in common wheat(Triticum aestivum L.)显示文摘Awns play an important role in seed dispersal and photosynthesis of spikes.Three major awn inhibitors(Hd,B1,and B2)are reported in wheat.However,the molecular mechanism underlying awnlessness remained unknown until recently.In this study,we identified two F8 recombinant inbred lines(RILs)that were segregating for awn length.In order to identify the causal gene for awn length in the heterozygous inbred families(HIFs),SNPs were called from RNA sequencing(RNA-Seq)data for HIF-derived progenies with long and short awns.SNPs between long and short awn plants were evenly distributed on chromosomes(chr)other than chromosome 5 A.SNPs on chr 5 A were clustered in a region distal 688 Mb on the long arm,where inhibitor B1 was located.This suggested that B1 was the causal segregating locus.We precisely mapped B1 to^1 Mb region using two HIF-derived families.Considering that the lines segregated for long,intermediate and short awn phenotypes we speculated that B1 should have a dosage effect on awn length.Two differentially expressed genes(DEGs)located in the candidate region were regarded as candidate genes for B1,because the molecular expression pattern was consistent with the phenotype.HIFs with long and short awns showed no difference on grain yield and other agronomic traits. | Jianqing Niu Shusong Zheng Xiaoli Shi Yaoqi Si Shuiquan Tian Yilin He Hong-Qing Ling | 2020 | The Crop Journal2020，8，4： | 0 |
| 6 | Progress in Research Methods on “Fluid-Diagenesis” Interactions of Fractures in Tight Reservoirs显示文摘1 Introduction Fractures in tight reservoirs have always attracted general petroleum geologists’attentions due to their importance in the processes of hydrocarbon enrichment and oil-gas exploration.Fractures can not only act as main pathways of hydrocarbon migration and fluid flow,but also provide major reservoir spaces for | WANG Xintao CHEN Yong ZHOU Yaoqi YUE Huiwen HE Chuan | 2015 | Acta Geologica Sinica(English Edition)2015，89，A01： | 0 |
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