| 1 | Natural astaxanthin enhanced antioxidant capacity and improved semen quality through the MAPK/Nrf2 pathway in aging layer breeder roosters显示文摘Background:Natural astaxanthin(ASTA)has strong antioxidant properties and has been widely used as a health product to improve human health.However,the effects of ASTA on the reproductive performance of aging roosters have been poorly studied.We aimed to investigate the effects of dietary ASTA on semen quality and antioxidant capacity in aging roosters and to explore the potential mechanism of semen quality change via antioxidation defense system.Methods:In the present study,9653-week-old Jinghong No.1 layer breeder roosters were fed a corn-soybean meal basal diet containing 0,25,50,or 100 mg/kg ASTA for 6 weeks.Results:Semen quality in the ASTA groups remarkably improved than that in the control group,and antioxidant activities,the abilities to scavenge hydroxyl radicals and superoxide anions,increased gradually with ASTA addition(P<0.05).In addition,the mRNA levels of antioxidant enzymes as well as the mRNA and protein levels of the mitogen-activated protein kinase(MAPK)and nuclear factor-erythroid 2-related factor 2(Nrf2)were markedly increased in the 50-100 mg/kg ASTA group(P<0.05).Conclusions:Collectively,these results demonstrate that dietary ASTA may improve semen quality by increasing antioxidant enzyme activities and the ability to scavenge hydroxyl radicals,which may be related to upregulation of the MAPK/Nrf2 pathway. | Shan Gao Nuo Heng Fang Liu Yong Guo Yu Chen Liang Wang Hemin Ni Xihui Sheng Xiangguo Wang Kai Xing Longfei Xiao Xiaolong Qi | 2022 | Journal of Animal Science and Biotechnology2022，13，2： | 2 |
| 2 | Exosomal miR-218 regulates the development of endometritis in dairy cows by targeting TGIF2/TGF-βpathway显示文摘Endometritis affects the reproductive capacity of dairy cows and leads to serious economic losses in dairy farming.Clarification of the pathogenesis of endometritis is necessary to improve the reproductive efficiency of dairy cows.Exosomes and their miRNAs have been proven to play an important role in inflammatory regulation.Exosomal miR-218 is a differentially expressed miRNA found in endometrial epithelial cells(EECs)under endometrial inflammation.Therefore,we investigated the expression of miR-218 in the uterine tissue of dairy cows,lipopolysaccharide(LPS)treated EECs,exosomal vesicles,and regulation of exosomal miR-218 by targeting TGIF-2 inducible factor homology frame 2(TGIF2)/transforming growth factor-beta(TGF-β).The expression of miR-218 was suppressed in inflammatory uterine tissues and LPS treated EECs.The expression of TGIF2 and TGF-βin inflammatory uterine tissues and LPS treated EECs was significantly higher than those in healthy uterine tissues and EECs(p<0.01).Interestingly,miR-218 derived from donor cells was found to regulate the expression of the target gene TGIF2 in recipient cells through the fusion of exosomes.Concurrently,the expression of its target gene TGIF2 was also suppressed by miR-218 in donor cells resulting in fewer TGIF2 being transported into recipient cells with exosomal fusion.This may be a novel mechanism of miRNAs-mediated regulation and provides a new reference for analyzing the pathogenesis of endometritis in dairy cows. | CHANG CHEN LIMIN QIAO KAIJUN GUO YINGQIU WANG MENGYI YUAN BOFAN FU XIAOBO GAO HEMIN NI LONGFEI XIAO XIANGGUO WANG | 2022 | BIOCELL2022，46，11： | 0 |
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