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| 1 | Adventitious Roots and Secondary Metabolism显示文摘Plants are a rich source of valuable secondary metabolites and in the recent years plant cell, tissue and organ cultures have been developed as an important alternative sources for the production of these compounds. Adventitious roots have been successfully induced in many plant species and cultured for the production of high value secondary metabolites of pharmaceutical, nutraceutical and industrial importance. Adoption of elicitation methods have shown improved synthesis of secondary metabolites in adventitious root cultures. Development of large-scale culture methods using bioreactors has opened up feasibilities of production of secondary metabolites at the industrial levels. In the present review we summarize the progress made in recent past in the area of adventitious root cultures for the production of secondary metabolites. | Hosakatte Niranjana Murthy Eun Joo Hahn Kee Yoeup Paek | 2008 | 生物工程学报2008，24，5： | 7 |
| 2 | Establishment of Withania somnifera Hairy Root Cultures for the Production of Withanolide A显示文摘Withania sominifera (Indian ginseng) was transformed by Agrobacterium rhizogenes. Explants from seedling roots, stems, hypocotyls, cotyledonary nodal segments, cotyledons and young leaves were inoculated with A. rhizogenes strain R1601. Hairy (transformed) roots were induced from cotyledons and leaf explants. The transgenic status of hairy roots was confirmed by polymerase chain reaction using nptII and rolB specific primers and, subsequently, by Southern analysis for the presence of nptII and rolB genes in the genomes of transformed roots. Four clones of hairy roots were established; these differed in their morphology. The doubling time of faster growing cultures was 8-14 d with a fivefold increase in biomass after 28 d compared with cultured, non-transformed seedling roots. MS-based liquid medium was superior for the growth of transformed roots compared with other culture media evaluated (SH, LS and N6), with MS-based medium supplemented with 40 g/L sucrose being optimal for biomass production. Cultured hairy roots synthesized withanolide A, a steroidal lactone of medicinal and therapeutic value. The concentration of withanolide A in transformed roots (157.4 μg/g dry weight) was 2.7-fold more than in non-transformed cultured roots (57.9 μg/g dry weight). | Hosakatte N. Murthy Camelia Dijkstra Paul Anthony Daniel A. White Mike R. Davey J. Brian Power Eun J. Hahn Kee Y. Paek | 2008 | Journal of Integrative Plant Biology2008，50，8： | 3 |
| 3 | Ginsenoside production by hairy root cultures of Panax ginseng: influence of temperature and light quality 显示文摘 | Kee W Y Hosakatte N M Eun J H | 2005 | Biochem Eng J2005，23，1： | 1 |
| 4 | Ginsenoside production by hairy root cultures of Panax ginseng- influence of temperature and light quality 显示文摘 | Kee-Won Yu Hosakatte N M Eun-Joo H | 2005 | Biochemical Engineering Journal2005，23，1： | 1 |
| 5 | Sucrose-induced osmotic stress affects biomass, metabolite, and antioxidant levels in root suspension cultures of Hypericum perforatum L.显示文摘 | Xi-Hua Cui Hosakatte Niranjana Murthy Chun-Hua Wu Kee-Yoeup Paek | 2010 | Plant Cell Tissue and Organ Culture (PCTOC)2010，，1： | 1 |
| 6 | Effect of oxygen supply on cell growth and saponin production in bioreactor cultures of Panax ginseng显示文摘 | Nguyen Trung Thanh Hosakatte Niranjana Murthy Kee-Won Yu Cheol Seung Jeong Eun-Joo Hahn Kee-Yoeup Paek | 2005 | Journal of Plant Physiology2005，，12： | 1 |
| 7 | Rapid propagation of Phalaenosis from floral stalk-derived leaves显示文摘 | PARK S Y HOSAKATTE N M PEAK K Y | | 0，，： | 1 |
| 8 | The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants显示文摘 | Kong Sik Shin Hosakatte Niranjana Murthy Jeong Wook Heo Eun Joo Hahn Kee Yoeup Paek | 2008 | Acta Physiologiae Plantarum2008，，3： | 1 |
| 9 | Ginsenoside production by hairy root cultures of Panax ginseng-influence of temperature and light quality显示文摘 | Kee-Won Yu Hosakatte N M Eun-Joo H | 2005 | Biochemical Engineering Journal2005，23，1： | 1 |
| 10 | Sucrose-in- duced osmotic stress affects biomass,metabolite, and antiox- idant levels in root suspension cultures of Hypericum pe'o- ratum显示文摘 | Cui Xihua Hosakatte N M Wu Chunhua | 2010 | Plant Cell Tiss Organ Cult2010，103，： | 1 |
| 11 | Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift reactor显示文摘 | Xi-Hua Cui Hosakatte Niranjana Murthy You-Xun Jin Yong-Hyeon Yim Ji-Yeong Kim Kee-Yoeup Paek | 2011 | Bioresource Technology2011，，21： | 1 |
| 12 | Micropropagation of yellow mangosteen:a valuable endemic tree of India显示文摘We developed a method for in vitro regeneration of Garcinia xanthochymus(yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant(WP) medium supplemented with cytokinins.An average of 11 shoots per explant were regenerated from mature seed segments on WP medium containing 20 μM 6-benzylaminopurine. Histological analysis revealed that hypodermal cells of seed segments were initially involved in active division, which later developed into meristemoids, subsequently leading to the formation of shoot buds.Shoot elongation was achieved by repeated subculturing of seed explants in shoot regeneration medium. Rooting of shoots was achieved on WP medium supplemented with indole-3-butyric acid or a-naphthalene acetic acid. Plantlets were transplanted to pots containing soil: compost(1:1) and survival rate was 90%. | Lalita Mahantagouda Patil Hosakatte Niranjana Murthy Vijayalaxmi S.Dandin Sunita P.Koli Shirugumbi Hanamanthagouda Manohar Shivanand D.Payamalle | 2016 | Journal of Forestry Research2016，27，1： | 1 |
| 13 | Ginsenoside production by hairy root cultures of Panax ginseng:influence of temperature and light quality显示文摘 | YU Keewon Hosakatte Niranjana Murthy EunJoo Hahn | 2005 | Biochemical Engineering Journal2005，23，1： | 1 |
| 14 | The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants 显示文摘 | Kong S S Hosakatte N M Jeong W H | 2008 | Acta Physiol Plant2008，30，： | 1 |
| 15 | Effects of sucrose and pH levels on in vitro shoot regeneration from leaf explants of Bacopa monnieri and accumulation of bacoside A in regenerated shoots显示文摘 | Poornananda Madhava Naik Shirugumbi Hanamanthagouda Manohar Nagella Praveen Hosakatte Niranjana Murthy | 2010 | Plant Cell Tissue and Organ Culture (PCTOC)2010，，2： | 1 |
| 16 | The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants显示文摘 | Kong S S Hosakatte N M Jeong W H | 2008 | Acta Physiologiae Plantarum2008，30，3： | 1 |
| 17 | The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants 显示文摘 | Kong SS Hosakatte NM Jeong WH | 2008 | Acta Physiol Plant2008，30，： | 1 |
| 18 | Parthenogenetic haploid plants using gamma irradiated pollen in snapmelon (Cucumis melo var. momordica)显示文摘 | Manoj Godbole Hosakatte Niranjana Murthy | 2012 | Plant Cell Tissue and Organ Culture (PCTOC)2012，，1： | 1 |
| 19 | The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants显示文摘 | Kong S S Hosakatte N M Jeong W H | 2008 | Acta Physiologiae Plantarum2008，30，： | 1 |
| 20 | Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate显示文摘 | Kim Yun-soo Hahn Eun-joo Murthy Hosakatte Niranjana | 2004 | Biotechnology Letters2004，26，21： | 1 |
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