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| 1 | HMGB 1 contributes to allergen-induced airway remodeling in a murine model of chronic asthma by modulating airway inflammation and activating lung fibroblasts显示文摘支持发炎的因素(HMGB1 ) 高活动性的组盒子蛋白质 1 在气喘的致病被含有。在这研究,我们使用了长期的气喘的一个鼠科的模型在航线改变上评估 HMGB1 的效果。女 BALB/c 老鼠随机被划分成四个组:控制, ovalbumin (卵) 气喘, OVA+适应于不同地区生活的动物抗体和 OVA+ anti-HMGB1 抗体。Anti-HMGB1 抗体治疗在白天 21 上被开始并且与卵在 intranasal 挑战前每星期被管理三次 6 个星期。在这个鼠标模型, HMGB1 表示显著地被提高。anti-HMGB1 抗体组展出了免疫球蛋白 E (IgE ) 和煽动性的调停人的减少的层次并且与卵组相比减少了煽动性的房间累积,航线 hyperresponsiveness (AHR ) ,粘液合成,光滑的肌肉厚度和肺骨胶原内容。有 HMGB1 的治疗增加了增长,移植,骨胶原分泌物和 α在 MRC-5 房间的光滑的肌肉肌动朊(SMA ) 表示。有 HMGB1/IL-1β 的处理;建筑群显著地增加了转变生长因素的表示和分泌物(TGF-β 1 ) ,矩阵 metalloproteinase (MMP )-9 和脉管的 endothelial 生长因素(VEGF ) 。总的来说,这些结果建议那项堵住的 HMGB1 活动可以颠倒航线由压制航线发炎和 modulating 肺成纤维细胞显型和激活改变。 | Changchun H ou Jinliang Kong Yue Liang Hong Huang Hanchun Wen Xiaowen Zheng Lihong Wu Yiqiang Chen | 2015 | Cellular & Molecular Immunology2015，12，4： | 19 |
| 2 | Therapeutic effect of Guijiajiao (Colla Carapacis et Plastri) on bone regeneration in rats and zebrafish显示文摘OBJECTIVE:To investigate the effect of Guijiajiao(Colla Carapacis et Plastri, CCP), the glue of tortoise shell, on bone regeneration in zebrafish and male Wistar rats.METHODS: In this study, we applied in vitro and in vivo models, tissue section analysis, and quantitative polymerase chain reaction to assess the effects of CCP on bone repair. MG-63 cells were used in alkaline phosphatase(ALP) activity and mineralization assays. Zebrafish and male Wistar rats were used to evaluate the effects of CCP on bone repair in vivo.RESULTS: A simple preparation of CCP promoted osteogenesis in vivo and in vitro, and promoted MG-63 cell proliferation as well as ALP activity and mineralization. In addition, CCP activated Akt and extracellular signaling-regulated kinase pathways and significantly increased the expression of ossification-related genes and proteins such as runt-related transcription factor-2, osteocalcin, and osteopontin. The in vivo results revealed promotion of osteogenesis by immersing zebrafish in CCP for 72 h.An oral dose of 1.25 g/kg CCP significantly improved skull defects in rats, which was accompanied by an increase in serum ALP levels.CONCLUSION: One of the ingredients of Guilu Erxianjiao(tortoise shell and deer horn formula) pro-vides a practical alternative therapy for bone regeneration. | Su Weiyang Feng Chienwei Tseng Chungchih Hung Hanchun Chen Zhicheng Lee Hsinpai Jean Yenhsuan Wu Shingyi Sean Wen Zhihong | 2018 | Journal of Traditional Chinese Medicine2018，38，2： | 3 |
| 3 | Identification of Nonstructural Protein 8 as the N-Terminus of the RNA-Dependent RNA Polymerase of Porcine Reproductive and Respiratory Syndrome Virus显示文摘Porcine reproductive and respiratory syndrome virus (PRRSV) is a member within the family Arteriviridae of the order Nidovirales. Replication of this positive-stranded RNA virus within the host cell involves expression of viral replicase proteins encoded by two ORFs, namely ORF1 a and ORF1 b. In particular, translation of ORF1 b depends on a-1-ribosomal frameshift strategy. Thus, nonstructural protein 9 (nsp9), the first protein within ORF1 b that specifies the function of the viral RNA-dependent RNA polymerase, is expressed as the C-terminal extension of nsp8, a small nsp that is encoded by ORF1 a. However, it has remained unclear whether the mature form of nsp9 in virus-infected cells still retains nsp8,addressing which is clearly critical to understand the biological function of nsp9. By taking advantage of specific antibodies to both nsp8 and nsp9, we report the following findings. (1) In infected cells, PRRSV nsp9 was identified as a major product with a size between 72 and 95 k Da (72–95 KDa form), which exhibited the similar mobility on the gel to the in vitro expressed nsp8–9 ORF1 b, but not the ORF1 b-coded portion (nsp9 ORF1 b). (2) The antibodies to nsp8, but not to nsp7 or nsp10, could detect a major product that had the similar mobility to the 72–95 KDa form of nsp9. Moreover, nsp9 could be co-immunoprecipitated by antibodies to nsp8, and vice versa. (3) Neither nsp4 nor nsp2 PLP2 was able to cleave nsp8–nsp9 in vitro. Together, our studies provide experimental evidence to suggest that nsp8 is an N-terminal extension of nsp9.Our findings here paves way for further charactering the biological function of PRRSV nsp9. | Yuanyuan Liu Yunhao Hu Yue Chai Liping Liu Jiangwei Song Shaochuan Zhou Jia Su Lei Zhou Xinna Ge Xin Guo Jun Han Hanchun Yang | 2018 | Virologica Sinica2018，33，5： | 3 |
| 4 | Nsp2 and GP5-M of Porcine Reproductive and Respiratory Syndrome Virus Contribute to Targets for Neutralizing Antibodies显示文摘Porcine reproductive and respiratory syndrome virus(PRRSV)is characterized by its genetic variation and limited cross protection among heterologous strains.Even though several viral structural proteins have been regarded as inducers of neutralizing antibodies(NAs)against PRRSV,the mechanism underlying limited cross-neutralization among heterologous strains is still controversial.In the present study,examinations of NA cross reaction between a highly pathogenic PRRSV(HP-PRRSV)strain,JXwn06,and a low pathogenic PRRSV(LP-PRRSV)strain,HB-1/3.9,were conducted with viral neutralization assays in MARC-145 cells.None of the JXwn06-hyperimmuned pigs’sera could neutralize HB-1/3.9 in vitro and vice versa.To address the genetic variation between these two viruses that are associated with limited crossneutralization,chimeric viruses with coding regions swapped between these two strains were constructed.Viral neutralization assays indicated that variations in nonstructural protein 2(nsp2)and structural proteins together contribute to weak cross-neutralization activity between JXwn06 and HB-1/3.9.Furthermore,we substituted the nsp2-,glycoprotein2(GP2)-,GP3-,and GP4-coding regions together,or nsp2-,GP5-,and membrane(M)protein-coding regions simultaneously between these two viruses to construct chimeric viruses to test cross-neutralization reactivity with hyperimmunized sera induced by their parental viruses.The results indicated that the swapped nsp2 and GP5-M viruses increased the neutralization reactivity with the donor strain antisera in MARC-145 cells.Taken together,these results show that variations in nsp2 and GP5-M correlate with the limited neutralization reactivity between the heterologous strains HP-PRRSV JXwn06 and LP-PRRSV HB-1/3.9. | Jia Su Lei Zhou Bicheng He Xinhui Zhang Xinna Ge Jun Han Xin Guo Hanchun Yang | 2019 | Virologica Sinica2019，34，6： | 3 |
| 5 | Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2显示文摘 | Kaichuang Shi Huanrong Li Xin Guo Xinna Ge Hong Jia Shijun Zheng Hanchun Yang | 2007 | Veterinary Microbiology2007，，3： | 2 |
| 6 | Molecular characterization of the genome of duck enteritis virus显示文摘 | Yufeng Li Bing Huang Xiuli Ma Jing Wu Feng Li Wu Ai Minxun Song Hanchun Yang | 2009 | Virology2009，，2： | 2 |
| 7 | Delta-shocks and vacuums in zero-pressure gas dynamics by the flux approximation显示文摘In this paper,firstly,by solving the Riemann problem of the zero-pressure flow in gas dynamics with a flux approximation,we construct parameterized delta-shock and constant density solutions,then we show that,as the flux perturbation vanishes,they converge to the delta-shock and vacuum state solutions of the zero-pressure flow,respectively.Secondly,we solve the Riemann problem of the Euler equations of isentropic gas dynamics with a double parameter flux approximation including pressure.Furthermore,we rigorously prove that,as the two-parameter flux perturbation vanishes,any Riemann solution containing two shock waves tends to a delta-shock solution to the zero-pressure flow;any Riemann solution containing two rarefaction waves tends to a two-contact-discontinuity solution to the zero-pressure flow and the nonvacuum intermediate state in between tends to a vacuum state.Finally,numerical results are given to present the formation processes of delta shock waves and vacuum states. | YANG HanChun LIU JinJing | 2015 | Science China Mathematics2015，58，11： | 2 |
| 8 | Porcine reproductive and respiratory syndrome in China显示文摘 | Lei Zhou Hanchun Yang | 2010 | Virus Research2010，，1： | 2 |
| 9 | Alteration of encephalomyocarditis virus pathogenicity due to a mutation at position 100 of VP1显示文摘Encephalomyocarditis virus (EMCV) infection leads to many diseases including encephalitis,myocarditis and diabetes in its natural host,the mouse.In this study,we generated four cDNA clones with a point mutation at position 100 of VP1.The amino acids isoleucine,alanine,serine and proline were substituted with threonine in the four different clones of EMCV strain BJC3 by site-specific mutagenesis,and viable viruses were rescued.Although all mutants and wild-type viruses display different plaque morphologies,they replicate comparably in BHK-21 cells.The pathogenicity of the mutated viruses was systematically analyzed to investigate the importance of this amino acid in the viral pathogenicity and disease phenotype of EMCV infection in mice.The results showed that the isoleucine(T1100I) and proline-mutated viruses (T1100P) exhibited a reduced mortality,lower cerebral virus loads and alleviated brain damage while the viruses with serine (T1100S) and alanine (T1100A) substitutions displayed similar properties as the wild-type virus.These findings indicate that the amino acid at position 100 of VP1 is important for EMCV in vivo infection,and its mutation alters the pathogenicity of viral infection in mice. | ZHU Shu GE XinNa GONG XiaoWen GUO Xin CHEN YanHong YANG HanChun | 2011 | Science China(Life Sciences)2011，54，6： | 2 |
| 10 | Quercetin-derivatives painting the yellow petals of American lotus(Nelumbo lutea)and enzymatic basis for their accumulation显示文摘American lotus(Nelumbo lutea)is one of the two species in Nelumbo and has only yellow flower.Identification of total flavonoids showed wild American lotus contained almost only flavonols with quercetin 3-O-glucuronide to be the dominant pigment.The variation tendency of the total flavonol content was coincident with yellow color variation of petals during flower development.To understand the mechanism of accumulation and constituent of pigments in petals,three pivotal genes,NlFLS1,NlFLS2 and NlFLS3,which were predicted to encode flavonol synthases were isolated and characterized by analyses of basic bioinformatics,temporal and spatial expression patterns and enzymatic activity.Their temporal expression levels showed the same variation tendency,which was also consistent with the development-dependent variation of total flavonol content.Spatial expression patterns indicated the three genes should function in petals.All the three proteins were demonstrated to be bifunctional dioxygenase,possessing both flavonol synthase activity and flavanone 3-hydroxylase activity.Besides,other flavonol biosynthesis related genes were also investigated on their expression levels to give more clues on the mechanism.Substrate preferences of the three FLSs,substrate competitions between the FLSs and other flavonol biosynthesis related enzymes,and the greatly differential expression levels between F3’H(flavonoid 3-hydroxylase)and F3’5’H(flavonoid 3,5-hydroxylase)contributed to the flavonol constituent in the petals of America lotus,namely abundant quercetin-derivatives while very few kaempferol-derivatives and myricetin-derivatives. | Qingqing Liu Dasheng Zhang Fengluan Liu Zhuoxing Liu Xiaohan Wang Yong Yang Shanshan Li Hanchun Li Daike Tian Liangsheng Wang | 2023 | Horticultural Plant Journal2023，9，1： | 1 |
| 11 | Characterization of multiple-antimicrobial-resistant Eacherichia coli isolates from diseased chickens and swine in China显示文摘 | Yang Hanchun Chen Sheng David G | 2004 | Journal of clinical microbiology2004，42，8： | 1 |
| 12 | Molecular variation analysis of porcine reproductive and respiratory syndrome virus in China显示文摘 | Lei Zhou Shuxian Chen Jialong Zhang Jingwen Zeng Xin Guo Xinna Ge Dabing Zhang Hanchun Yang | 2009 | Virus Research2009，，1： | 1 |
| 13 | Three-dimensional scene reconstruction by using lenslet array model in com- putational integral imaging显示文摘 | Huang Yusheng Xia Jun Yin Hanchun | 2009 | Proceedings of SPIE2009，，75： | 1 |
| 14 | Genotyping of porcine circovirus type 2 from a variety of clinical conditions in China显示文摘 | Libin Wen Xin Guo Hanchun Yang | 2005 | Veterinary Microbiology2005，110，： | 1 |
| 15 | Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China显示文摘 | Yueyi Gao Qiuwen Kou Xinna Ge Lei Zhou Xin Guo Hanchun Yang | 2013 | Archives of Virology2013，，3： | 1 |
| 16 | Characterization of muhiple-antimicrobial-resistant Escherichia coli isolates from diseased chickens and swine in China显示文摘 | Hanchun Y Sheng C David G W | 2004 | J Clini Microbiol2004，42，8： | 1 |
| 17 | Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells显示文摘 | Qinghao Liu Yixian Qin Lei Zhou Qiuwen Kou Xin Guo Xinna Ge Hanchun Yang Hongbo Hu | 2012 | Virology2012，，2： | 1 |
| 18 | Giant persistent current in a quantum ring with multiple arms 显示文摘 | Wu Hanchun Guo Yong Chen Xinyi | 2003 | Phys Rev B2003，68，： | 1 |
| 19 | Generalized plane delta shock waves for n-dimensional zero-pressure gas dynamics显示文摘 | | 2001 | J Mathematical Analysis and Applications2001，260，： | 1 |
| 20 | Motion adaptive debluring filter for LCD 显示文摘 | Jun Xia Yue Shi Hanchun Yin | 2009 | Displays2009，30，： | 1 |
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