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| 1 | Graft-accelerated virus-induced gene silencing facilitates functional genomics in rose flowers显示文摘Rose has emerged as a model ornamental plant for studies of flower development, senescence, and morphology, as well as the metabolism of floral fragrances and colors.Virus-induced gene silencing(VIGS) has long been used in functional genomics studies of rose by vacuum infiltration of cuttings or seedlings with an Agrobacterium suspension carrying TRV-derived vectors. However, VIGS in rose flowers remains a challenge because of its low efficiency and long time to establish silencing. Here we present a novel and rapid VIGS method that can be used to analyze gene function in rose,called ‘graft-accelerated VIGS', where axil ary sprouts are cut from the rose plant and vacuum infiltrated with Agrobacterium. The inoculated scions are then grafted back onto the plants to flower and silencing phenotypes can be observed within 5 weeks, post-infiltration. Using this new method, we successfully silenced expression of the RhDFR_1, RhA G, and RhNUDX_1 in rose flowers, and affected their color, petal number, as well as fragrance, respectively. This grafting method will facilitate high-throughput functional analysis of genes in rose flowers. Importantly, it may also be applied to other woody species that are not currently amenable to VIGS by conventional leaf or plantlet/seedling infiltration methods. | Huijun Yan Shaochuan Shi Nan Ma Xiaoqian Cao Hao Zhang Xianqin qiu Qigang Wang Hongying Jian Ningning Zhou Zhao Zhang Kaixue Tang | 2018 | Journal of Integrative Plant Biology2018，60，1： | 11 |
| 2 | Inheritance of Perpetual Blooming in Rosa chinensis ‘Old Blush'显示文摘Perpetual blooming is one of the most important biological and economical traits in modern rose, while the genetic basis underlining the control of this trait is poorly investigated. With an aim in dissecting the genetic determinism of perpetual blooming, we developed six rose populations(OB, W, F1, F2, BC1 OB and BC1W) derived from a WOB population [interspecific diploid hybridization between Rosa chinensis ‘Old Blush'(OB) and R. wichuriana ‘Basye's Thornless'(W)]. Perpetual blooming is absent both in a F1 population with 296 individuals and a BC1 W population(W as the backcross parent) with 150 individuals. However, the perpetual blooming trait showed a typical 3︰1 segregation in a backcross population BC1 OB with OB as the backcross parent. In this population with 300 individuals, 83 plants had the perpetual blooming phenotype while the other 217 featured non-perpetual blooming, indicating that the perpetual blooming trait is very likely controlled by two recessive genes in R. chinensis(rpb1 and rpb2). These genetic data suggest that the inheritance of rose perpetual blooming may be controlled by a complex mechanism. | LI Shubin ZHOU Ningning ZHOU Qing YAN Huijun JIAN Hongying WANG Qigang CHEN Min QIU Xianqin ZHANG Hao WANG Shufang LI Shufa TANG Kaixue | 2015 | Horticultural Plant Journal2015，1，2： | 9 |
| 3 | Photosynthesis and metabolite responses of Isatis indigotica Fortune to elevated [CO2]显示文摘Climate change is affecting global crop productivity, food quality, and security. However,few studies have addressed the mechanism by which elevated CO_2 may affect the growth of medicinal plants. Isatis indigotica Fortune is a widely used Chinese medicinal herb with multiple pharmacological properties. To investigate the physiological mechanism of I.indigotica response to elevated [CO_2], plants were grown at either ambient [CO_2](385 μmol mol^(-1)) or elevated [CO_2] (590 μmol mol^(-1)) in an open-top chamber (OTC)experimental facility in North China. A significant reduction in transpiration rate (T_r) and stomatal conductance (g_s) and a large increase in water-use efficiency contributed to an increase in net photosynthetic rate (Pn) under elevated [CO_2] 76 days after sowing. Leaf non-photochemical quenching (NPQ) was decreased, so that more energy was used in effective quantum yield of PSII photochemistry (Φ_(PSⅡ)) under elevated [CO_2]. High ΦPSII,meaning high electron transfer efficiency, also increased Pn. The [CO_2]-induced increase in photosynthesis significantly increased biomass by 36.8%. Amounts of metabolic compounds involved in sucrose metabolism, pyrimidine metabolism, flavonoid biosynthesis, and other processes in leaves were reduced under elevated [CO_2]. These results showed that the fertilization effect of elevated [CO_2] is conducive to increasing dry weight but not secondary metabolism in I. indigotica. | Ping Li Hongying Li Yuzheng Zong Frank Yonghong Li Yuanhuai Han Xingyu Hao | 2017 | The Crop Journal2017，5，4： | 2 |
| 4 | Ligand assisted copper-catalyzed Ullmann cross coupling reaction of bromaminic acid with amines显示文摘Three types of ligands have been developed for copper-catalyzed Ullmann cross coupling reaction of bromaminic acid with amines in aqueous solution. Ligands with large steric hindrance and strong electron-donating capacity were beneficial to the reaction. UV–Vis and CV analyses demonstrated that these ligands had strong coordination with copper(I), implying the effect of ligand coordination ability on the stability and catalytic activity of catalytic system. | Beibei Shao Hongying Du Xinyu Hao Rongwen Lu Yi Luo Shufen Zhang | 2016 | Chinese Journal of Chemical Engineering2016，24，8： | 2 |
| 5 | Generation of Nb-enriched mafic rocks and associated adakitic rocks from the southeastern Central Asian Orogenic Belt:Evidence of crust-mantle interaction显示文摘Melting of subducting oceanic lithosphere and associated melt-mantle interactions in convergent plate margins require specific geodynamic environment that allows the oceanic slab to be abnormally heated.Here we focus on the Early Mesozoic mafic rocks and granite porphyry,which provide insights into slab melting processes associated with final closure of the Paleo-Asian Ocean.The granite porphyry samples are calc-alkaline and distinguished by high Sr contents,strong depletion of heavy rare earth elements,resulting in high(La/Yb);and Sr/Y ratios,and negligible Eu anomalies.Based on their high Na_(2)O and Mg O,low K_(2)O contents,positiveε_(Hf)(t)andε_(Nd)(t)and low(^(87)Sr/^(86)Sr)ivalues,we propose that the granite porphyry was likely derived from partial melting of subducting Paleo-Asian oceanic crust.The Nb-enriched mafic rocks are enriched in Rb,Th,U,Pb and K,and depleted in Nb,Ta,Ba,P and Ti,corroborating a subduction-related origin.Their heterogeneous Sr-Nd-Hf-O isotopic compositions and other geochemical features suggest that they were likely derived from partial melting of peridotitic mantle wedge interacted with oceanic slab-derived adakitic melts.Trace element and isotope modeling results and elevated zirconδ^(18)O values suggest variable subducting sediments input into the mantle wedge,dominated by terrigenous sediments.Synthesizing the widely-developed bimodal rock associations,conjugated dikes,thermal metamorphism,tectonic characteristics,paleomagnetic constraints,and paleogeographical evidence along the Solonke-Changchun suture zone,we identify a slab window triggered by slab break-off,which accounts for slab melting and formation of the Nb-enriched mafic rocks and associated adakitic granite porphyry in southeastern Central Asian Orogenic Belt. | Yan Jing Wenchun Ge M.Santosh Yu Dong Hao Yang Zheng Ji Junhui Bi Hongying Zhou Dehe Xing | 2022 | Geoscience Frontiers2022，13，2： | 2 |
| 6 | SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants显示文摘RNA quality control nonsense-mediated decay is involved in viral restriction in both plants and animals.However,it is not known whether two other RNA quality control pathways,nonstop decay and no-go decay,are capable of restricting viruses in plants.Here,we show that the evolutionarily conserved Pelota–Hbs1 complex negatively regulates infection of plant viruses in the family Potyviridae(termed potyvirids),the largest group of plant RNA viruses that accounts for more than half of the viral crop damage worldwide.Pelota enables the recognition of the functional G1-2A6-7 motif in the P3 cistron,which is conserved in almost all potyvirids.This allows Pelota to target the virus and act as a viral restriction factor.Furthermore,Pelota interacts with the SUMO E2-conjugating enzyme SCE1 and is SUMOylated in planta.Blocking Pelota SUMOylation disrupts the ability to recruit Hbs1 and inhibits viral RNA degradation.These findings reveal the functional importance of Pelota SUMOylation during the infection of potyvirids in plants. | Linhao Ge Buwei Cao Rui Qiao Hongguang Cui Shaofang Li Hongying Shan Pan Gong Mingzhen Zhang Hao Li Aiming Wang Xueping Zhou Fangfang Li | 2023 | Molecular Plant2023，16，3： | 2 |
| 7 | A Comparative Study of Electrochemical Capacitance of Graphene Oxide Affected by Oligomers of p-phenylenediamine and Hydrazine Hydrate in Solvothermal Condition显示文摘A systematic investigation of the graphene oxide composite reduced by either p-phenylenediamine oligomers or hydrazine hydrate was performed with field emission scanning electron microscopy,high resolution transmission electron microscopy,X-ray diffraction,fourier transform infrared,and X-ray photoelectron spectroscopy analyses.The electrical capacitance of the composite was evaluated by a cyclic voltammetry technique,while the properties of these prototype supercapacitors were measured by a chronopotentiometry technique.It was found that,under the solvothermal condition,the graphene oxide reduced by p-phenylenediamine oligomers was observed to have higher electrical capacitance than that reduced by hydrazine.The improved electrical capacitance can be attributed to that p-phenylenediamine oligomers are the more effective spacers for graphene layers;and they could also provide some pseudo-capacitance to the graphene oxide composite based on their conjugate structure.The results imply that graphene oxide modified by diamine oligomers has a good potential in energy storage devices. | ZHANG Qingqing ZHANG Qingwu LU Hao WANG Wei FANG Tian LIU Hongying LIU Jiaji WANG Shuangshuang | 2020 | Journal of Wuhan University of Technology(Materials Science)2020，35，2： | 2 |
| 8 | Non-isothermal microwave leaching kinetics and absorption characteristics of primary titanium-rich materials显示文摘 | Xia Hongying Peng Jinhui Niu Hao | 2010 | Transactions of Nonferrous Metals Society of China2010，20，： | 1 |
| 9 | Investigation of the rheological modification mechanism of crumb rubber modified asphalt (CRMA) containing TOR additive显示文摘 | Hongying Liu Zhijun Chen Wen Wang Hainian Wang Peiwen Hao | 2013 | Construction and Building Materials2013，，： | 1 |
| 10 | Investigation on specific heat capacity and thermal behavior of sodium hydroxyethyl sulfonate显示文摘The thermal decomposition process was studied by the TG–DTA analyzer. The results show that the decomposition process of sodium hydroxyethyl sulfonate consisted of three stages: the mass loss for the first, the second and third stages may be about the groups of CH_3CH_2OH, CH_3CHO and SO_2 volatilized, respectively. The decomposition residuum of three stages was analyzed by FT-IR, and the results of FT-IR agreed with the decomposition process predicted by theoretical weight loss. The specific heat capacity of sodium hydroxyethyl sulfonate was determined by differential scanning calorimetry(DSC). The melting temperature and melting enthalpy were obtained to be 465.41 K and 25.69 kJ·mol^(-1), respectively. The molar specific heat capacity of sodium hydroxyethyl sulfonate was determinated from 310.15 K to 365.15 K and expressed as a function of temperature. | Hongying Hao Yadong Zhang Xiaoya Chen | 2017 | Chinese Journal of Chemical Engineering2017，25，3： | 1 |
| 11 | The electrochemical performance of carboxymethyl cellulose with different DS as a binding material in lithium batteries显示文摘 | Hao Hongying Shao Ziqiang | 2012 | Adv Mater2012，499，： | 1 |
| 12 | Non - isothermal microwave leaching kinetics and absorption characteristics of primary titanium- rich materials显示文摘 | Xia Hongying Peng Jinhui Niu Hao | 2010 | Transaction of Nonferrous Metals Society of China2010，20，： | 1 |
| 13 | TPE based aggregation induced emission fluorescent sensors for viscosity of liquid and mechanical properties of hydrogel显示文摘Two amphiphilic TPE E/Z isomers with aggregation induced emission(AIE)property have been synthesized and characterized.The logarithmic fluorescent intensity of the two molecules was in positive relationship with logarithmic viscosity of liquid.To note,the Z-TPE isomer exhibited more sensitivity in the viscosity of liquid sensing in comparison with the corresponding E-TPE counterpart(around 1.80 folds).Furthermore,two molecules could be used as fluorescent sensors for mechanical properties(viscosity and storage modulus)of hydrogel as well.In addition,two sensors displayed low cytotoxicity in normal tissue cell line(L929)within the concentration range of 2–10μmol/L.These results potentially promised their applications as fluorescent sensors for mechanical properties in the fields of biological and biomedical. | Na Wang Hang Yao Qi Tao Jing Sun Hao Ma Yang Wang ChengCheng Zhou Hongying Fan Hongxia Shao Aijian Qin Dawei Su Chenyin Wang Hui Chong | 2022 | Chinese Chemical Letters2022，33，1： | 1 |
| 14 | Decaploidy in Rosa praelucens Byhouwer (Rosaceae) Endemic to Zhongdian Plateau, Yunnan, China显示文摘 | Hongying Jian Hao Zhang Kaixue Tang Shufa Li Qigang Wang Ting Zhang Xianqin Qiu Huijun Yan | 2010 | Caryologia2010，，2： | 1 |
| 15 | Final-stage Southward Subduction of the Eastern Paleo-Asian Ocean:Evidence from the Middle Permian Mafic Intrusions in the Northern Margin of the North China Craton显示文摘The northern margin of the North China Craton(NCC)contains widespread Permian magmatic rocks,but the origin of these rocks remains controversial.This uncertainty hampers us from better understanding of tectonic framework and evolution of the eastern Paleo-Asian Ocean,particularly with respect to its final-stage subduction and closure time.To address these questions,this study presents petrological,zircon U-Pb geochronological,whole-rock geochemical and in situ zircon Hf isotopic data for these Permian mafic intrusions in the northern margin of the NCC.Precise zircon U-Pb dating results indicate that these mafic intrusions were emplaced in the Middle Permian(ca.260 Ma).Geochemically,the studied mafic intrusions have high MgO and transition metals element contents,with high Mg^(#) values,indicating a mantle origin.These mafic intrusions are characterized by enrichments in large ion lithophile elements(LILEs;e.g.,Rb,Ba,and K)and light rare earth elements(LREEs),and depletions in high field strength elements(HFSEs;e.g.,Nb,Ta,and Ti)and heavy rare earth elements(HREEs),indicating that they were formed in a subduction-related setting.These geochemical features,together with zircon ε_(Hf)(t)values(-1.1 to+11.2),indicate that their parental magmas were derived from partial melting of heterogeneous mantle wedge metasomatized by subduction-related fluids,with the contributions of slab sediments.The studied mafic intrusions also show wide range of major and trace elements contents,and variable Mg^(#) values,Eu and Sr anomalies,suggesting that their parental magmas had undergone variable degrees of fractional crystallization.Together with the E-W trending Permian continental arc along the northern margin of the NCC,we confirm that the generation of the Middle Permian mafic intrusions was related to southward subduction of the Paleo-Asian oceanic lithosphere beneath the NCC and the Paleo-Asian Ocean had not closed prior to the Middle Permian. | JING Yan GE Wenchun DONG Yu YANG Hao JI Zheng BI Junhui ZHOU Hongying XING Dehe | 2022 | Acta Geologica Sinica(English Edition)2022，96，1： | 1 |
| 16 | Fuzzy Neural Very-short-term Load Forecasting Based on Chaotic Dynamics Reconstruction显示文摘 | Yang Hongying Ye Hao Wang Guizeng | 2006 | Chaos Solitons & Fractals2006，29，2： | 1 |
| 17 | Feed weight estimation model for health monitoring of meat rabbits based on deep learning显示文摘With the development of precision livestock farming,non-contact health monitoring technology is particularly important in the breeding process.To help improve the management of the rabbit breeding industry,a remaining feed weight(RFW)estimation model was developed based on the image segmentation method.The model proposed in this study consisted of a feed instance segmentation neural network and feed weight estimation network.Feed instance segmentation neural network was based on the improved Mask Region-based Convolution Neural Network(Mask RCNN),the state-of-art image segmentation method,and the PointRend algorithm was used to replace the original network head.Through an adaptive subdivision strategy,the boundary points were segmented with fine details.Features were extracted from the segmentation results and used as the input of the feed weight estimation network based on the Back Propagation(BP)algorithm.The model was applied in rabbit breeding to explore the relationship between RFW and the mortality probability of meat rabbits.The model evaluation results showed that the Average Precision(AP)value of the feed instance segmentation neural network was 0.987,the Mean Pixel Accuracy(MPA)value was 0.985.The correlation coefficient of the feed weight estimation network was 0.97,the Mean Squared Error(MSE)was 208.3,and the Mean Absolute Error(MAE)was 10.51 g.The practical application results showed that the feed intake of the unhealthy meat rabbits would decrease significantly.When the RFW was more than 50%of feed quantity,the mortality probability of the rabbit was more than 85%;when the RFW was more than 65%of feed quantity,all the rabbits finally died in a short time.Therefore,there is a significant correlation between RFW and the mortality probability of rabbits,by which this proposed model can help farms to monitor the health of meat rabbits by predicting RFW. | Enze Duan Liangju Wang Hongying Wang Hongyun Hao Rangling Li | 2022 | International Journal of Agricultural and Biological Engineering2022，15，1： | 0 |
| 18 | Functional characterization of novel NPRL3 mutations identified in three families with focal epilepsy显示文摘Focal epilepsy accounts for 60% of all forms of epilepsy, but the pathogenic mechanism is not well understood. In this study,three novel mutations in NPRL3(nitrogen permease regulator-like 3), c.937_945del, c.1514dup C and 6,706-bp genomic DNA(g DNA) deletion, were identified in three families with focal epilepsy by linkage analysis, whole exome sequencing(WES) and Sanger sequencing. NPRL3 protein is a component of the GATOR1 complex, a major inhibitor of m TOR signaling. These mutations led to truncation of the NPRL3 protein and hampered the binding between NPRL3 and DEPDC5, which is another component of the GATOR1 complex. Consequently, the mutant proteins enhanced m TOR signaling in cultured cells, possibly due to impaired inhibition of m TORC1 by GATOR1. Knockdown of nprl3 in Drosophila resulted in epilepsy-like behavior and abnormal synaptic development. Taken together, these findings expand the genotypic spectrum of NPRL3-associated focal epilepsy and provide further insight into how NPRL3 mutations lead to epilepsy. | Shiyue Du Sheng Zeng Li Song Hongying Ma Rui Chen Junyu Luo Xu Wang Tingbin Ma Xuan Xu Hao Sun Ping Yi Jifeng Guo Yaling Huang Mugen Liu Tao Wang Wei-Ping Liao Luoying Zhang Jing Yu Liu Beisha Tang | 2023 | Science China(Life Sciences)2023，66，9： | 0 |
| 19 | Comparative transcriptomic and metabonomic analysis revealed the relationships between biosynthesis of volatiles and flavonoid metabolites in Rosa rugosa显示文摘Rosa rugosa is not only cultivated as a landscaping plant,but also used in cosmetics,the medical and food industries.However,little information is currently available on the gene regulatory networks involved in its scent and color biosynthesis and metabolism.In this study,R.rugosa Thunb.f.rosea Rehd with red petals(RR)and its white petal variant(WR),were used to study the molecular mechanisms in flower color and scent.Sixty-five differential flavonoid metabolites and 15 volatiles were found to have significant differences between RR and WR.Correspondingly,the key regulators(MYB-bHLH-WD40)of anthocyanin synthesis pathway and their structural genes involved in flavonoid biosynthesis,benzenoid/phenylpropanoid biosynthesis,terpenoid biosynthesis pathways were also found to be differentially expressed by comparative transcriptome.Further,qPCR permitted the identification of some transcripts encoding proteins that were putatively associated with scent and color biosynthesis in roses.Particularly,the results showed that the ACT gene(encoding CoA geraniol/citronellol acetyltransferase,GeneID:112190420),which expressed lower in WR,was involved in three pathways:flavonoid biosynthesis,phenylpropanoid biosynthesis and terpenoid biosynthesis,however,GT5(anthocyanin glycosylation gene,GeneID:112186660),expressed higher in WR,was involved in both flavonoid and phenylpropanoid biosynthesis pathways.These results suggested that ACT and GT5 might play important roles in regulating the relationship of color pigmentation and volatile emission. | Dedang Feng Hao Zhang Xianqin Qiu Hongying Jian Qigang Wang Ningning Zhou Yu Ye Jun Lu Huijun Yan Kaixue Tang | 2021 | Ornamental Plant Research2021，1，1： | 0 |
| 20 | 血压变化和空气污染微粒的化学成分显示文摘[背景]血压升高与颗粒物(PM)空气污染相关,但是与PM化学成分的关联尚不明确。[目的]通过对一组39名大学生进行460次反复访查,探讨血压与不同化学成分的细颗粒物(PM2.5)之间的关联。 | Shaowei Wu Furong Deng Jing Huang Hongyi Wang Masayuki Shima Xin Wang Yu Qin Chanjuan Zheng Hongying Wei Yu Hao Haibo Lv Xiuling Lu Xinbiao Guo | 2013 | 环境与职业医学2013，30，4： | 0 |
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