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| 1 | Germline mutation analysis of MLH1 and MSH2 in Malaysian Lynch syndrome patients显示文摘AIM: To investigate the protein expression profi le of mismatch repair (MMR) genes in suspected cases of Lynch syndrome and to characterize the associated germline mutations. METHODS: Immunohistochemical analysis of tumor samples was performed to determine the protein expression profile of MMR protein. Germline mutation screening was carried out on peripheral blood samples. The entire exon regions of MLH1 and MSH2 geneswere amplifi ed by polymerase chain reaction, screened by denaturing high performance liquid chromatography (dHPLC) and analyzed by DNA sequencing to characterize the germline mutations. RESULTS: Three out of 34 tissue samples (8.8%) and four out of 34 tissue samples (11.8%) showed loss of nuclear staining by immunohistochemistry, indicating the absence of MLH1 and MSH2 protein expression in carcinoma cells, respectively. dHPLC analysis followed by DNA sequencing showed these samples to have germline mutations of MSH2 gene. However, no deleterious mutations were identifi ed in any of the 19 exons or coding regions of MLH1 gene, but we were able to identify MLH1 promoter polymorphism, -93G > A (rs1800734), in 21 out of 34 patients (61.8%). We identified one novel mutation, transversion mutation c.2005G > C, which resulted in a missense mutation (Gly669Arg), a transversion mutation in exon 1, c.142G > T, which resulted in a nonsense mutation (Glu48Stop) and splice-site mutation, c.2006-6T > C, which was adjacent to exon 13 of MSH2 gene. CONCLUSION: Germline mutations were identified in four Malaysian Lynch syndrome patients. Immunohistochemical analysis of tumor tissue proved to be a good pre-screening test before proceeding to germline mutation analysis of DNA MMR genes. | Mohd Nizam Zahary Ravindran Ankathi Human Genome Centre Gurjeet Kaur Muhammad Radzi Abu Hassan Harjinder Singh Venkatesh R Naik | 2012 | World Journal of Gastroenterology2012，18，8： | 3 |
| 2 | A new green approach for the synthesis of 12-aryl-8,9,10,12-tetrahydrobenzo[a]xanthene-11-one derivatives using task specific acidic ionic liquid[NMP]H_2PO_4显示文摘12-Aryl-8,9,10,12-tetrahydrobenzo[a]xanthene-11-one derivatives were synthesized by a convenient and environmentally benign procedure involving multicomponent condensation reactions of substituted aromatic aldehydes with 2,7-dihydroxynaphthalene/2-naphthols/2,6-dihydroxynaphthlene and cyclic 1,3-dicarbonyl compounds in task specific acidic ionic liquid[NMP]H_2PO_4 at 80 ℃.This protocol has proved to be efficient in terms of good yields,operational simplicity,easy workup,recyclability of reaction medium/catalyst,and short reaction time. | Harjinder Singh Sudesh Kumari Jitender M.Khurana | 2014 | Chinese Chemical Letters2014，25，10： | 3 |
| 3 | Mycoplasma pneumoniae in children with acute and refractory asthma显示文摘 | Pamela R. Wood Vanessa L. Hill Margaret L. Burks Jay I. Peters Harjinder Singh Thirumalai R. Kannan Shruthi Vale Marianna P. Cagle Molly F.R. Principe Joel B. Baseman Edward G. Brooks | 2013 | Annals of Allergy Asthma & Immunology2013，，5： | 2 |
| 4 | Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery显示文摘 | Anil Kumar Anal Harjinder Singh | 2007 | Trends in Food Science & Technology2007，，5： | 2 |
| 5 | The milk fat globule membrane—A biophysical system for food applications显示文摘 | Harjinder Singh | 2005 | Current Opinion in Colloid & Interface Science2005，，2： | 2 |
| 6 | Physicochemical changes in intermediate-moisture protein bars made with whey protein or calcium caseinate显示文摘 | Simon M. Loveday Jason P. Hindmarsh Lawrence K. Creamer Harjinder Singh | 2010 | Food Research International2010，，5： | 1 |
| 7 | Efficient,green and regioselective synthesis of 1,4,5-trisubstituted-1,2,3-triazoles in ionic liquidBF4and in task-specific basic ionic liquidOH显示文摘 | HARJINDER S JAYANT S JITENDER K | 2013 | Journal of The Iranian Chemical Society2013，10，1007： | 1 |
| 8 | The effect of cooking and washing temperature during pilot-scale rennet casein manufacture on casein hydration characteristics in disodium orthophosphate solution显示文摘 | Michele M Harjinder S Peter A M | | 0，，01： | 1 |
| 9 | Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery 显示文摘 | ANIL K A HARJINDER S | 2007 | Trend Food Sei Teeh2007，18，5： | 1 |
| 10 | Characterisation of a high acyl gellan polysaccharide using light scattering and rheological techniques显示文摘 | Kelvin K.T. Goh Derek R. Haisman Harjinder Singh | 2005 | Food Hydrocolloids2005，，2： | 1 |
| 11 | Effects of Heat and High Hydrostatic Pressure Treatents on Disulfide Bonding Interchanges among the Proteins in Skim Milk显示文摘 | Hasmukh A Patel Harjinder S | 2006 | J Agric Food Chem2006，，54： | 1 |
| 12 | Intheritance of forage quality and other agronomic traits in maize,te- osinte and their hybrids显示文摘 | Harjinder S Bhardwaj B L Gupta B K | 2004 | Crop Improvement2004，31，1： | 1 |
| 13 | Selective solid-phase extraction of rare earth elements by the chemically modified Amberlite XAD-4 resin with azacrown ether 显示文摘 | Sudhir R D Harjinder K Shobhana K M | 2010 | Reactive andFunctional Polymers2010，70，9： | 1 |
| 14 | Multicomponent domino process for the synthesis of some novel 5-(arylidene)-3-((1-aryl-1H-1,2,3-triazol-4-yl)methyl)-thiazolidine-2,4-diones using PEG-400 as an efficient reaction medium and their antimicrobial evaluation显示文摘A series of novel thiazolidinedione-triazole hybrids were synthesized by one pot reaction between thiazolidine-2.4-dione,substituted aryl aldehydes,piopargyl bromide and substituted aryl azides using piperidine,CuSO_4· 5H_2O and sodium ascorbate as catalysts in PEG-400 as a highly efficient and green media.These thiazolidinedione-triazole hybrids were subjected to in vitro antibacterial activity against four strains namely.Staphylococcus aureus.Bacillus subtilis,Escherichia coli,Pseudomonas aeruginosa and antifungal activity against two fungal strains namely,Aspergillus niger and Aspergillus flavus. | Jayant Sindhu Harjinder Singh J.M.Khurana Chetan Sharma K.R.Aneja | 2015 | Chinese Chemical Letters2015，26，1： | 1 |
| 15 | Pituitary metastasis from medullary carcinoma of thyroid: case report and review of literature显示文摘 | Harjinder S. Bhatoe Sonia Badwal Vibha Dutta N. Kannan | 2008 | Journal of Neuro - Oncology2008，，1： | 1 |
| 16 | Recent advances in microencapsulation of probiotics for Industrial applications and targeted delivery 显示文摘 | Anil Kumar Anal Harjinder Singh | 2007 | Trends in Food Science and Technology2007，18，： | 1 |
| 17 | Structure and integrity of liposomes prepared from milk- or soybean-derived phospholipids during in vitro digestion显示文摘 | Weilin Liu Aiqian Ye Chengmei Liu Wei Liu Harjinder Singh | 2012 | Food Research International2012，，2： | 1 |
| 18 | Effect of the fat globule membrane on in vitro digestion of milk fat globules with pancreatic lipase显示文摘 | Aiqian Ye Jian Cui Harjinder Singh | 2010 | International Dairy Journal2010，，12： | 1 |
| 19 | Acute propafenone toxicity after two exposures at standard dosing 显示文摘 | ADAM Y DAVID S HARJINDER P | 2010 | Canadian Journal of Cardiology2010，26，6： | 1 |
| 20 | Hydrolysis of milk phospholipid and phospholipid–protein monolayers by pancreatic phospholipase A 2显示文摘 | Sophie Gallier Ethan Shaw Julia Cuthbert Derek Gragson Harjinder Singh Rafael Jiménez-Flores | 2013 | Food Research International2013，，： | 1 |
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