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| 1 | Polymerase chain reaction of Au nanoparticle-bound primers显示文摘Polymerase chain reaction (PCR) is a useful technique for in vitro amplification of a DNA fragment. In this paper, a PCR procedure using Au nanoparticle (AuNP) -bound primers was systemically studied. The 5′-SH- (CH2)6-modified primers were covalently attached to the AuNP surface via Au-S bonds, and plasmid pBluescript SK was used as a template. The effects of the concentration of AuNP-bound primers, annealing temperature and PCR cy-cles were evaluated, respectively. The results indicate that PCR can proceed successfully under optimized condition, with either forward or reverse primers bound to the AuNP surface or with both the two primers bound to the AuNP surface. Development of PCR procedure based on AuNPs not only makes the isolation of PCR products very convenient, but also provides novel methods to prepare AuNP-bound ssDNA and nanostructured material. | SHEN Hebai HU Min YANG Zhongnan WANG Chen ZHU Longzhang | 2005 | Chinese Science Bulletin2005，50，18： | 4 |
| 2 | Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191显示文摘抄写因素的 DNA 有约束力的性质上的研究在寻找这些因素调整的下游的基因是重要的。在现在的学习,我们在一个 Cy s2His2 类型抄写因素的 DNA 有约束力的性质上报导,锌手指蛋白质 191 (Zfpl91 ) ,它最新被发现了在老鼠起一个重要作用。由构造包含 Zfpl91 的 DNA 有约束力的域的熔化蛋白质,我们由在锌上决定蛋白质的有约束力的特性和依赖描绘了目标 DNA。数据证明 Zfpl91 的 DNA 有约束力的域能明确地绑在 TCAT 重复主题并且在目标 DNA 的多重有约束力的地点之中有合作效果。而且,有约束力的反应依赖于锌。这个工作在基因规定和开发在 Zfpl91 的角色上为进一步的研究提供一个基础。 | Haoyue Wang Ruilin Sun Guoxiang Liu Minghui Yao Jian Fei Hebai Shen | 2008 | Acta Biochimica et Biophysica Sinica2008，40，8： | 3 |
| 3 | Electrochemical properties of ordered mesoporous carbon and its electroanalytical application for selective determination of dopamine显示文摘 | Nengqin Jia Zhiyong Wang Guofeng Yang Hebai Shen Longzhang Zhu | 2006 | Electrochemistry Communications2006，，2： | 1 |
| 4 | Synthesis of magnetic and fluorescent bifunctional nanocomposites and their applications in detection of lung cancer cells in humans显示文摘 | Jingwei Ma Qishi Fan Lianhui Wang Nengqin Jia Zhidong Gu Hebai Shen | 2010 | Talanta2010，，4： | 1 |
| 5 | Kinetics of Titanium( IV ) Chloride Oxidation 显示文摘 | Sotiris E P Bai Hebai | 1990 | J Am Ceram Soc1990，，73： | 1 |
| 6 | Hydrolysis of oligodeoxynucleotide phosphodiester linkages显示文摘 | Hebai Shen Jingfen Xia Haifeng Yang Yumei Luo | 2001 | Science in China Series B: Chemistry2001，，2： | 1 |
| 7 | Specific hydrolysis of DNA by Ce^(4+)-ODN hybrid显示文摘The cerium complex bound to the derivative of oligoDNA has been synthesized successfully that can hydrolyze DNA with sequence-specificity. The synthesized derivative of oligoDNA, 5′-EDTA-P-10 mers ODN, was demonstrated by HPLC. The fluorescence spectrum of Tb3+ was detected after its interaction with the hybrid of 10-mers ODN and 26-mers ODN and the results show that the artificial endo-enzyme can recognize and combine firmly with the substrate DNA. The electrophorogram shows that the cerium-oligoDNA hybrid can specifically hydrolyze its substrate DNA and the cleavage site of this hydrolysis reaction is also discussed. This artificial nuclease can be widely used in molecular biology and genetic engineering as one kind of endo-enzyme. | SHEN Hebai ZHOU Wenjun YANG Yongtao ZHANG Feng | 2004 | Science China Chemistry2004，47，1： | 0 |
| 8 | SYNTHESIS AND CHARACTERIZATION OF THE SOLID COMPLEXES OF Mn(Ⅱ), Co(Ⅱ), Cu(Ⅱ), Zn(Ⅱ), Ag(Ⅰ), Cd(Ⅱ) NITRATES WITH DISUBSTITUTED BENZO CROWN ETHERS显示文摘Nine new solid complexes of Mn(Ⅱ),Co(Ⅱ),Cu(Ⅱ),Zn(Ⅱ.),Ag(Ⅰ) nitrates with 4’,5’-dibromobenzo-15—crown—5(LA),Ag(Ⅰ),Cd(Ⅱ)nitrates with tetrabromodibenzo—18—crown—6(LB)and Cu(Ⅱ), Ag(Ⅰ) nitrates with 4’—bromo—5’—nitrobenzo—15—crown-5(Lc),having the compositions of M(NO2)2·L·nH2O(M=Mn,Co, Zn,L=LA,n=2;M=Cu,L=LA,n=1.5; M=Cd, L=LB,n=0; M=Cu,L=Lc,n=2) and AgNO·L·nCH3CN(L=LA,n=0; L=LB,n=1; L=Lc,n=1/2) have been synthesized in nonaqueous solvent. All the isolated complexes have been characterized by elementary analysis, IR and UV spectra, differential thermal and thermogravimetric analysis, X—ray powder diffraction analysis, molar conductance and measuremends of solubility in some general solvents. | Ai Ru LU, Yu Jie DAI~(**), You Ming ZHANG~*, Lian Xiang ZHANG Department of Chemistry, Northwest Normal University, Lanzhou, 730070 Liu Fang WANG Department of Chemistry, Lanzhou University, 730000 *Author for correspondence **Hebai Medical Institute | 1992 | Chinese Chemical Letters1992，3，10： | 0 |
| 9 | THE HIDDEN DANGERS IN CITY CONSTRUCTION AND THEIR PREVENTION STRATEGIES | Liu Baocai and Zhao Zhilin (The Construction Commission of Hebai Province) | 1996 | Natural Disaster Reduction in China1996，，2： | 0 |
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