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| 1 | Two outward potassium current types are expressed during the neural differentiation of neural stem cells显示文摘The electrophysiological properties of potassium ion channels are regarded as a basic index for determining the functional differentiation of neural stem cells.In this study,neural stem cells from the hippocampus of newborn rats were induced to differentiate with neurotrophic growth factor,and the electrophysiological properties of the voltage-gated potassium ion channels were observed.Immunofluorescence staining showed that the rapidly proliferating neural stem cells formed spheres in vitro that expressed high levels of nestin.The differentiated neurons were shown to express neuron-specific enolase.Flow cytometric analysis revealed that the neural stem cells were actively dividing and the percentage of cells in the S+G2/M phase was high.However,the ratio of cells in the S+G2/M phase decreased obviously as differentiation proceeded.Whole-cell patch-clamp recordings revealed apparent changes in potassium ion currents as the neurons differentiated.The potassium ion currents consisted of one transient outward potassium ion current and one delayed rectifier potassium ion current,which were blocked by 4-aminopyridine and tetraethylammonium,respectively.The experimental findings indicate that neural stem cells from newborn rat hippocampus could be cultured and induced to differentiate into functional neurons under defined conditions in vitro.The differentiated neurons expressed two types of outward potassium ion currents similar to those of mature neurons in vivo. | Ruiying Bai Guowei Gao Ying Xing Hong Xue | 2013 | Neural Regeneration Research2013，8，28： | 3 |
| 2 | Complete DNA sequence and gene analysis of the virulence plasmid pCP301 of Shigella flexneri 2a显示文摘 | Jiyu Zhang Hong Liu Xiaobing Zhang Jian Yang Fan Yang Guowei Yang Yan Shen Yunde Hou Qi Jin | 2003 | Science in China Series C: Life Sciences2003，，5： | 1 |
| 3 | A Study of Digital Camera Colorimetric Characterization Based on Polynomial Modeling显示文摘 | Hong Guowei Luo Ronnier M Rhodes Peter A | 2001 | Color Research and Application2001，26，3： | 1 |
| 4 | Assessment of structure damage to blasting induced显示文摘 | Guowei Ma Hong Hao Yingxin Zhou | 2000 | Engineering Structures2000，，22： | 1 |
| 5 | Homogenization of masom'y using numerical simulations显示文摘 | Guowei Ma Hong Hao Yong Lu | 2001 | Journal of Eng- ingeering Mechanies ASCE2001，127，5： | 1 |
| 6 | Assessment of strueture damage to blasting indueed 显示文摘 | Guowei Ma Hong Hao Yingxin Zhou | 2000 | Engineering Struetures2000，，22： | 1 |
| 7 | Homogenization of Masonry Using Numerical Simulations 显示文摘 | Ma Guowei Hao Hong Lu Yong | 2001 | ASCE Journal of Engineering Mechanics2001，127，5： | 1 |
| 8 | Assessment of structure damage to blasting induced ground motions显示文摘 | Ma Guowei Hao Hong Zhou Yingxin | 2000 | Engineering Structures2000，，22： | 1 |
| 9 | Sphingosine kinase 1 promotes tumor immune evasion by regulating the MTA3-PD-L1 axis显示文摘Immune checkpoint blockade(ICB)exhibits considerable benefits in malignancies,but its overall response rate is limited.Previous studies have shown that sphingosine kinases(SPHKs)are critical in the tumor microenvironment(TME),but their role in immunotherapy is unclear.We performed integrative analyses including bioinformatics analysis,functional study,and clinical validation to investigate the role of SPHK1 in tumor immunity.Functionally,we demonstrated that the inhibition of SPHK1 significantly suppressed tumor growth by promoting antitumor immunity in immunocompetent melanoma mouse models and tumor T-cell cocultures.A mechanistic analysis revealed that MTA3 functions as the downstream target of SPHK1 in transcriptionally regulating tumor PD-L1.Preclinically,we found that anti-PD-1 monoclonal antibody(mAb)treatment significantly rescued tumor SPHK1 overexpression or tumor MTA3 overexpression-mediated immune evasion.Significantly,we identified SPHK1 and MTA3 as biological markers for predicting the efficacy of anti-PD-1 mAb therapy in melanoma patients.Our findings revealed a novel role for SPHK1 in tumor evasion mediated by regulating the MTA3-PD-L1 axis,identified SPHK1 and MTA3 as predictors for assessing the efficacy of PD-1 mAb treatment,and provided a therapeutic possibility for the treatment of melanoma patients. | Poyee Lau Guanxiong Zhang Shuang Zhao Long Liang Hailun Zhang Guowei Zhou Mien-Chie Hung Xiang Chen Hong Liu | 2022 | Cellular & Molecular Immunology2022，19，10： | 1 |
| 10 | Damage assessment of masonry infilled RC frames subjected to blasting induced ground excitations显示文摘 | Hao Hong Ma Guowei Lu Yong | 2002 | Engineering Structure2002，，24： | 1 |
| 11 | Damage assessment of masonry infilled RC frames subjueted to blasting induced ground excitations显示文摘 | HAO Hong MA Guowei LU Yong | 2002 | Engineering Structures2002，24，6： | 1 |
| 12 | Homogenization of masonry using numerical simulations 显示文摘 | Ma Guowei Hao Hong Lu Youg | 2001 | ASCE Journal Engineering Mechanics2001，127，1： | 1 |
| 13 | Assessment of structure damage to blasting induced显示文摘 | Ma Guowei Hao Hong Zhou Yingxin | 2000 | Engineering Structures2000，，22： | 1 |
| 14 | Assessment of structure damage to blasting induced ground motions 显示文摘 | MA Guowei HAO Hong ZHOU Yingxin | 2000 | Engineering Structures2000，22，10： | 1 |
| 15 | Assessment of structure damage to blasting induced显示文摘 | Guowei Ma Hong Hao Yingxin Zhou | 2000 | Engineering Structures2000，，22： | 1 |
| 16 | Assessment of Structure Damage to Blasting Induced Ground Motions显示文摘 | Ma Guowei Hao Hong Zhou Yingxin | 2000 | Engineering Structures2000，，22： | 1 |
| 17 | Bi-layer collagen/microporous electrospun nanofiber scaffold improves the osteochondral regeneration显示文摘 | Shufang Zhang Longkun Chen Yangzi Jiang Youzhi Cai Guowei Xu Tong Tong Wei Zhang Linlin Wang Junfeng Ji Peihua Shi Hong Wei Ouyang | 2013 | Acta Biomaterialia2013，，7： | 1 |
| 18 | Assesment of structure damage to blasting induced ground motions显示文摘 | Guowei Ma Hong Hao Yingxin Zhou | 2001 | Engineering Structures2001，22，1： | 1 |
| 19 | Homogenization of masonry using numerial simulations显示文摘 | GUOWEI MA HONG HAO MEMBER ASCE YONG LU | 2001 | ASCE Journal of Engineering Mechanics2001，127，5： | 1 |
| 20 | Predicting chaotic time series with wavelet networks显示文摘 | Liangyue Cao Yiguang Hong Haiping Fang Guowei He | 1995 | Physica D: Nonlinear Phenomena1995，，1： | 1 |
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