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| 1 | Histological Subtypes of Lung Cancer in Chinese Males from 2000 to 2012显示文摘Objective To characterize the histological and epidemiological features of male lung cancer patients in China. Methods The demographic and histological information about male lung cancer patients identified from 2000-01-01 to 2012-12-31,was collected from the Cancer Hospital of the Chinese Academy of Medical Sciences. Relative frequencies(RF) were estimated for major histological subtypes and compared according to the years of diagnosis and birth. Results The RF of adenocarcinoma(ADC) increased from 21.96% to 43.36% and the RF of squamous cell carcinoma(SCC) decreased from 39.11% to 32.23% from 2000 to 2012 in the 15 427 male lung cancer patients included in this study(Z=17.909,P<0.0001; Z=-6.117,P<0.0001). The RF of ADC increased from 28.72% in 2000-2004,36.88% in 2005-2008 to 48.61% in 2009-2012 in patients born after 1960. The age-adjusted RF of ADC in 2007-2012 increased consistently in all the investigated areas. Conclusion The increased RF of ADC in male lung cancer patients highlights the need for further investigation of the etiologic factors of these tumors. Smoke-free policies rather than modifying tobacco products should be enforced. | ZOU Xiao Nong LIN Dong Mei WAN Xia CHAO Ann FENG Qin Fu DAI Zhen YANG Gong Huan LV Ning | 2014 | Biomedical and Environmental Sciences2014，27，1： | 19 |
| 2 | Bone marrow stromal cells as a therapeutic treatment for ischemic stroke显示文摘Cerebral ischemia remains the most frequent cause of death and quality-of-life impairments due to neurological deficits, and accounts for the majority of total healthcare costs. However, treatments for cerebral ischemia are limited. Over the last decade, bone marrow stromal cell(BMSC) therapy has emerged as a particularly appealing option, as it is possible to help patients even when initiated days or even weeks after the ischemic insult. BMSCs are a class of multipotent, self-renewing cells that give rise to differentiated progeny when implanted into appropriate tissues. Therapeutic effects of BMSC treatment for ischemic stroke, including sensory and motor recovery, have been reported in pre-clinical studies and clinical trials. In this article, we review the recent progress in BMSC-based therapy for ischemic stroke, focusing on the route of delivery and pre-processing of BMSCs. Selecting an optimal delivery route is of particular importance. The ideal approach, as well as the least risky, for translational applications still requires further identification. Appropriate preprocessing of BMSCs or combination therapy has the benefit of achieving the maximum possible restoration. Further pre-clinical studies are required to determine the time-window for transplantation and the appropriate dosage of cells. | Zizhen Yang Lei Zhu Fangqin Li Jing Wang Huan Wan Yujun Pan | 2014 | Neuroscience Bulletin2014，30，3： | 16 |
| 3 | A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice显示文摘The transient elevation of cytoplasmic calcium is essential for pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI).However,the calcium channels responsible for this process have remained unknown.Here,we show that rice CDSI(CELL DEATH and SUSCEPTIBLE to BLAST 1)encoding OsCNGC9;a cyclic nucleotide-gated channel protein,positively regulates the resistance to rice blast disease.We show that OsCNGC9 mediates PAMP-induced Ca2+influx and that this event is critical for PAMPs-triggered ROS burst and induction of PTI-related defense gene expression.We further show that a PTI related receptor-lih cytoplasmic kinase OsRLCK185 physically interacts with and phosphorylates OsCNGC9 to activate its channel activity.Our results suggest a signaling cascade linking pattern recognition to calcium channel activation,which is required for initiation of PTI and disease resistance in rice. | Jiachang Wang Xi Liu An Zhang Yulong Ren Fuqing Wu Gang Wang Yang Xu Cailin Lei Shanshan Zhu Tian Pan Yongfei Wang Huan Zhang Fan Wang Yan-Qiu Tan Yupeng Wang Xin Jin Sheng Luo Chunlei Zhou Xiao Zhang Jinling Liu Shuai Wang Lingzhi Meng Yihua Wang Xi Chen Qibing Lin Xin Zhang Xiuping Guo Zhijun Cheng Jiulin Wang Yunlu Tian Shijia Liu Ling Jiang Chuanyin Wu Ertao Wang Jian-Min Zhou Yong-Fei Wang Haiyang Wang Jianmin Wan | 2019 | Cell Research2019，29，10： | 13 |
| 4 | GW5-Like, a homolog of GW5, negatively regulates grain width, weight and salt resistance in rice显示文摘Grain size is an important determinant of yield potential in crops. We previously demonstrated that natural mutations in the regulatory sequences of qSW5/GW5 confer grain width diversity in rice. However, the biological function of a GW5 homolog, named GW5-Like(GW5 L), remains unknown. In this study, we report on GW5 L knockout mutants in Kitaake, a japonica cultivar(cv.)considered to have a weak gw5 variant allele that confers shorter and wider grains. GW5 L is evenly expressed in various tissues, and its protein product is localized to the plasma membrane. Biochemical assays verified that GW5 L functions in a similar fashion to GW5. It positively regulates brassinosteroid(BR) signaling through repression of the phosphorylation activity of GSK2. Genetic data show that GW5 L overexpression in either Kitaake or a GW5 knockout line, Kasaorf3(indica cv. Kasalath background), causes more slender, longer grains relative to the wild-type. We also show that GW5 L could confer salt stress resistance through an association with calmodulin protein OsCa M1-1. These findings identify GW5 L as a negative regulator of both grain size and salt stress tolerance, and provide a potential target for breeders to improve grain yield and salt stress resistance in rice. | Peng Tian Jiafan Liu Changling Mou Cuilan Shi Huan Zhang Zhichao Zhao Qibing Lin Jie Wang Jiulin Wang Xin Zhang Xiuping Guo Zhijun Cheng Shanshan Zhu Yulong Ren Cailin Lei Haiyang Wang Jianmin Wan | 2019 | Journal of Integrative Plant Biology2019，61，11： | 9 |
| 5 | Fractionated stereotactic radiotherapy with vagina carotica protection technique for local residual nasopharyngeal carcinoma after primary radiotherapy显示文摘 | LIU Feng XIAO Jian-ping XU Ying-jie ZHANG Ye XU Guo-zhen GAO Li YI Jun-lin LUO Jing-wei HUANG Xiao-dong HUAN Fu-kui FANG Hao WAN Bao LI Ye-xiong | 2012 | Chinese Medical Journal2012，，14： | 8 |
| 6 | Spatial Distribution of Liver Cancer Incidence in Shenqiu County,Henan Province,China:a Spatial Analysis显示文摘Liver cancer is a common and leading cause of cancer death in China.We used the cancer registry data collected from 2009 to 2011 to describe the spatial distribution of liver cancer incidence at village level in Shengqiu county,Henan province,China.Spatial autocorrelation analysis was employed to detect significant differences from a random spatial distribution of liver cancer incidence.Spatial scan statistics were used to detect and evaluate the clusters of liver cancer cases. | SUN Jie HUANG Hui XIAO Ge Xin FENG Guo Shuang YU Shi Cheng XUE Yu Tang WAN Xia YANG Gong Huan SUN Xin | 2015 | Biomedical and Environmental Sciences2015，28，3： | 7 |
| 7 | Effects of Yue-Bi-Tang on water metabolism in severe acute pancreatitis rats with acute lung-kidney injury显示文摘BACKGROUND The complications acute lung injury and acute kidney injury caused by severe inflammation are the main reasons of high mortality of severe acute pancreatitis(SAP).These two complications can both lead to water metabolism and acid-base balance disorders,which could act as additional critical factors affecting the disease trend.Aquaporins(AQPs),which can regulate the transmembrane water transport,have been proved to participate in the pathophysiological process of SAP and the associated complications,such as acute lung injury and acute kidney injury.Thus,exploring herbs that can effectively regulate the expression of AQP in SAP could benefit the prognosis of this disease.AIM To determine whether Yue-Bi-Tang(YBT)can regulate the water metabolism in rats with severe acute pancreatitis via regulating the expression of aquaporins.METHODS Sprague-Dawley rats were randomly divided into three groups,sham operation group(SOG),model group(MG),and treatment group(TG).SAP was induced with 3.5%sodium taurocholate in the MG and TG.Rats in the TG were administered with YBT while SOG and MG rats were given the same volume of saline.Blood and tissue samples were harvested to detect serum inflammatory cytokines,histopathological changes,malondialdehyde and superoxide dismutase in the lung,and protein and mRNA expression of kidney injury molecule-1,α-smooth muscle actin,and vimentin in the kidney,and AQP1 and 4 in the lung,pancreas,and kidney.RESULTS The serum interleukin-10,tumor necrosis factorα,and creatinine levels were higher in the MG than in the SOG.Tumor necrosis factorαlevel in the TG was lower than that in the MG.Malondialdehyde level in lung tissues was higher than in the SOG.The pathological scores and edema scores of the pancreas,lung,and kidney tissues in the MG were all higher than those in the SOG and TG.The protein expression of AQP4 in lung tissues and AQP1 in kidney tissues in the MG were higher than those in the SOG and TG.The expression of vimentin was significantly higher in the MG than in the SOG.The expression of AQP1 mRNA in the lung and kidney,and AQP4 mRNA in the kidney was up-regulated in the MG compared to the SOG.CONCLUSION YBT might regulate water metabolism to reduce lung and kidney edema of SAP rats via decreasing AQP expression,and alleviate the tissue inflammatory injury. | Jing Hu Yu-Mei Zhang Yi-Fan Miao Lv Zhu Xiao-Lin Yi Huan Chen Xi-Jing Yang Mei-Hua Wan Wen-Fu Tang | 2020 | World Journal of Gastroenterology2020，26，43： | 7 |
| 8 | Optimal timing for the oral administration of DaCheng-Qi decoction based on the pharmacokinetic and pharmacodynamic targeting of the pancreas in rats with acute pancreatitis显示文摘AIM To identify the optimal oral dosing time of Da-Cheng-Qi decoction(DCQD) in rats with acute pancreatitis(AP) based on the pharmacokinetic and pharmacodynamic parameters.METHODS First, 24 male Sprague-Dawley rats were divided into a sham-operated group [NG(a)] and three model groups [4 h G(a), 12 h G(a) and 24 h G(a)]. The NG(a) and model groups were administered DCQD(10 g/kg.BW) intragastrically at 4 h, 4 h, 12 h and 24 h, respectively, after AP models induced by 3% sodium taurocholate. Plasma samples were collected from the tails at 10 min, 20 min, 40 min, 1 h, 2 h, 4 h, 8 h, 12 h and 24 h after a single dosing with DCQD. Plasma and pancreatic tissue concentrations of the major components of DCQD were determined by high-performance liquid chromatography tandem mass spectroscopy. The pharmacokinetic parameters and serum amylase were detected and compared. Second, rats were divided into a sham-operated group [NG(b)] and three treatment groups [4 h G(b), 12 h G(b) and 24 h G(b)] with three corresponding control groups [MG(b)s]. Blood and pancreatic tissues were collected 24 h after a single dosing with DCQD. Serum amylase, inflammatory cytokines and pathological scores of pancreatic tissues were detected and compared.RESULTS The concentrations of emodin, naringin, honokiol, naringenin, aloe-emodin, chrysophanol and rheochrysidin in the 12 h G(a) group were higher than those in the 4 h G(a) group in the pancreatic tissues(P < 0.05). The area under the plasma concentration-time curve from time 0 to the time of the last measurable concentration values(AUC0→t) for rhein, chrysophanol, magnolol and naringin in the 12 h G(a) group were larger than those in the 4 h G(a) or 24 h G(a) groups. The 12 h G(a) group had a higher Cmax than the other two model groups. The IL-10 levels in the 12 h G(b) and 24 h G(b) groups were higher than in the MG(b)s(96.55 ± 7.84 vs 77.46 ± 7.42, 251.22 ± 16.15 vs 99.72 ± 4.7 respectively, P < 0.05), while in the 24 h G(b) group, the IL-10 level was higher than in the other two treatment groups(251.22 ± 16.15 vs 154.41 ± 12.09/96.55 ± 7.84, P < 0.05). The IL-6 levels displayed a decrease in the 4 h G(b) and 12 h G(b) groups compared to theMG(b)s(89.99 ± 4.61 vs 147.91 ± 4.36, 90.82 ± 5.34 vs 171.44 ± 13.43, P < 0.05). CONCLUSION Late-time dosing may have higher concentrations of the most major components of DCQD, with better pharmacokinetics and pharmacodynamics of antiinflammation than early-time dosing, which showed the late time to be the optimal dosing time of DCQD for AP. | Yu-Mei Zhang Lin Zhu Xian-Lin Zhao Huan Chen Hong-Xin Kang Jian-Lei Zhao Mei-Hua Wan Juan Li Lv Zhu Wen-Fu Tang | 2017 | World Journal of Gastroenterology2017，23，39： | 5 |
| 9 | Microstructure and properties of vacuum counter-pressure cast aluminum alloy显示文摘The microstructure and properties of vacuum counter-pressure cast aluminum alloy were studied. Results indicated that under the condition of vacuum counter-pressure, liquid melts fill mould cavity under the vacuum and crystallize under high pressure which have very good effect on nucleation and solidification feeding. Compared with gravity casting, the microstructure of vacuum counter-pressure cast aluminum alloy is much finer and more uniformly distributed. Mechanical properties of vacuum counter-pressure cast aluminum alloy are improved significantly. | YAN Qing-song YU Huan WEI Bo-kang XU Zhi-feng CAI Chang-chun WAN Hong | 2006 | China Foundry2006，3，2： | 5 |
| 10 | Heritability of growth traits in the Asian seabass (Lates calcarifer)显示文摘Growth is an economically important trait in aquaculture.To improve growth trait of the Asian seabass(Lates calcarifer)we have been carrying out,since 2004,a selective breeding program.This study focuses on growth traits in the F2 fish generation,comprised of offspring from 23 mass crosses from 383 F_(1) brooders.Using genotyping analysis for 10 microsatellites from both brood stock and progeny we have reconstructed the pedigree of each mass-cross.For F_(2) generation at 90 days post hatch(dph),we have recorded body weight(BW)for 12,117 individuals and total length(TL),standard length(SL)and condition factor(K_(tl) and K_(sl))for 3530 individuals;and all five traits for 2136 individuals at 270 dph.At 90 dph the average BW was 46.88±20.95 g.Combining pedigree information,recorded growth traits and Restricted Maximum Likelihood method,we have estimated that the narrow sense heritability(h^(2))in F_(1) fish for BW,TL,SL,K_(tl) and K_(sl) was at,90 dph,0.12±0.03,0.11±0.03,0.10±0.03,0.20±0.04 and 0.11±0.03,respectively and,at 270 dph,0.34±0.07,0.32±0.07,0.30±0.06,0.13±0.04 and 0.11±0.04,respectively.At 90 dph the realised heritability for BW was 0.13.Comparing with F_(1) generation,the growth performance of F_(2) fish was increased by 14.4%.Heritability of growth traits will be useful for future genetic improvement programmes of the Asian seabass. | Baoqing Ye Ziyi Wan Le Wang Hongyan Pang Yanfei Wen Huiming Liu Bing Liang Huan Sein Lim Junhui Jiang Genhua Yue | 2017 | Aquaculture and Fisheries2017，2，3： | 4 |
| 11 | Sheng-jiang powder ameliorates obesity-induced pancreatic inflammatory injury via stimulating activation of the AMPK signalling pathway in rats显示文摘AIM To investigate the mechanisms by which Sheng-jiang powder(SJP) ameliorates obesity-induced pancreatic inflammatory injury.METHODS Sprague-Dawley rats were randomized into three groups: normal group(NG), obese group(HLG), or SJP treatment group(HSG). Obesity was induced by feeding a high-fat diet in the HLG and HSG, while the NG received standard chow. Rats were euthanized after 12 wk, and blood and pancreatic tissues were collected for histopathological analyses. Nuclear factor kappa-light-chain-enhancer of activated B cells(NF-κB) and transforming growth factor beta(TGF-β) expression, serum triglyceride and adiponectin levels, and apoptosis in pancreatic acinar cells were assessed. A high-fat AR42 J acinar cell injury model was established using very low-density lipoprotein(VLDL). AR42 J acinar cell culture supernatant, treated with different interventions, was applied to seven groups of pancreatic stellate cells(PSCs). The proliferation of PSCs and the expression of fibronectin and type I collagenase were assessed.RESULTS Compared with the NG, we found higher pathological scores for pancreatic tissues, lower serum adiponectin levels, higher expression levels of NF-κB in pancreatic tissues and TGF-β in pancreatic inflammatory cells, and increased apoptosis among pancreatic acinar cells for the HLG(P < 0.05). Compared with the HLG, we found reduced body weight, Lee's index scores, serum triglyceride levels, and pathological scores for pancreatic tissues; higher serum adiponectin levels; and lower expression levels of NF-κB, in pancreatic tissue and TGF-β in pancreatic inflammatory cells for the HSG(P < 0.05). The in vitro studies showed enhanced PSC activation and increased expression levels of fibronectin and type I collagenase after SJP treatment. An adenosine 5‘-monophosphate-activated protein kinase(AMPK) inhibitor inhibited PSC activation.CONCLUSION SJP may ameliorate obesity-induced pancreatic inflammatory injury in rats by regulating key molecules of the adiponectin-AMPK signalling pathway. | Yi-Fan Miao Juan Li Yu-Mei Zhang Lv Zhu Huan Chen Ling Yuan Jing Hu Xiao-Lin Yi Qiu-Ting Wu Mei-Hua Wan Wen-Fu Tang | 2018 | World Journal of Gastroenterology2018，24，39： | 4 |
| 12 | Effect of Sheng-jiang powder on multiple-organ inflammatory injury in acute pancreatitis in rats fed a high-fat diet显示文摘BACKGROUND Obesity worsens inflammatory organ injury in acute pancreatitis(AP), but there is no effective preventive strategy. Sheng-jiang powder(SJP) has been shown to alleviate multiple-organ inflammatory injury in rats with high-fat diet-induced obesity. Hence, SJP is supposed to have an effect on multiple-organ inflammatory injury in AP in rats fed a high-fat diet.AIM To explore how obesity may contribute to aggravating inflammatory organ injury in AP in rats and observe the effect of SJP on multiple-organ inflammatory injury in AP in rats fed a high-fat diet.METHODS Rats were randomly assigned to a control group(CG), an obese group(OG), and an SJP treatment group(SG), with eight rats per group. The rats in the OG and SG were fed a high-fat diet. From the third week, the rats in the SG were given oral doses of SJP(5 g/kg of body weight). After 12 wk, AP was induced in the three groups. Serum amylase level, body weight, Lee's index, serum biochemistry parameters, and serum inflammatory cytokine and tissue cytokine levels were assessed, and the tissue histopathological scores were evaluated and compared.RESULTS Compared with the CG, serum triglyceride, total cholesterol, interleukin-6, and interleukin-10 levels were significantly higher in the OG, and serum high-density lipoprotein cholesterol level was significantly lower in the OG. Moreover,enhanced oxidative damage was observed in the pancreas, heart, spleen, lung,intestine, liver, and kidney. Evidence of an imbalanced antioxidant defense system, especially in the pancreas, spleen, and intestine, was observed in the obese AP rats. Compared with the OG, serum high-density lipoprotein cholesterol, interleukin-10, and superoxide dismutase expression levels in the pancreas, spleen, and intestine were increased in the SG. Additionally, SJP intervention led to a decrease in the following parameters: body weight; Lee's index; serum triglyceride levels; serum total cholesterol levels; malondialdehyde expression levels in the pancreas, heart, spleen, lung, and liver; myeloperoxidase expression levels in the lung; and pathological scores in the liver.CONCLUSION Obesity may aggravate the inflammatory reaction and pathological multipleorgan injury in AP rats, and SJP may alleviate multiple-organ inflammatory injury in AP in rats fed a high-fat diet. | Yi-Fan Miao Hong-Xin Kang Juan Li Yu-Mei Zhang Hong-Yu Ren Lv Zhu Huan Chen Ling Yuan Hang Su Mei-Hua Wan Wen-Fu Tang | 2019 | World Journal of Gastroenterology2019，25，6： | 4 |
| 13 | Genetic architecture and key genes controlling the diversity of oil composition in rice grains显示文摘Rice grain oil is a valuable nutrient source.However,the genetic basis of oil biosynthesis in rice grains remains unclear.In this study,we performed a genome-wide association study on oil composition and oil concentration in a diverse panel of 533 cultivated rice accessions.High variation for 11 oil-related traits was observed,and the oil composition of rice grains showed differentiation among the subpopulations.We identified 46 loci that are significantly associated with grain oil concentration or composition,16 of which were detected in three recombinant inbred line populations.Twenty-six candidate genes encoding enzymes involved in oil metabolism were identified from these 46 loci,four of which(PAL6,LIN6,MYR2,and ARA6)were found to contribute to natural variation in oil composition and to show differentiation among the subpopulations.Interestingly,population genetic analyses revealed that specific haplotypes of PAL6 and LIN6 have been selected in japonica rice.Based on these results,we propose a possible oil biosynthetic pathway in rice grains.Collectively,our results provide new insights into the genetic basis of oil biosynthesis in rice grains and can facilitate marker-based breeding of rice varieties with enhanced oil and grain quality. | Hao Zhou Duo Xia Pingbo Li Yiting Ao Xiaodong Xu Shanshan Wan Yanhua Li Bian Wu Huan Shi Kaiyue Wang Guanjun Gao Qinglu Zhang Gongwei Wang Jinghua Xiao Xianghua Li Sibin Yu Xingming Lian Yuqing He | 2021 | Molecular Plant2021，14，3： | 4 |
| 14 | Coal petrology and genesis of Jurassic coal in the Ordos Basin,China显示文摘简单结构和浅埋葬深度描绘的厚煤床的多重集合在 Ordos 盆的早、中间的侏罗记阶层被开发,西北的中国。这高质量的煤的巨大的储备有高商业的价值。我们学习了煤岩石学的特征和它的 maceral 分发到决定 macerals 贡献到油和气体的产生。结果证明因为主要是在上面的河的系统的一系列高看台的沼泽地的盆唯一的 depositional 环境,在 Ordos 盆的侏罗记煤有特殊 petrological 特征。这些 petrographic 特征是一些沙身体被移植河形成的典型内陆湖的发展的结果。在埋葬以后,泥炭继续经历氧化条件,产生广泛的更高的 inertinite 在煤满足的这个过程和 vitrinite 部件被改变到 semi-vitrinite。这些侏罗记煤的宏观的 petrographic 类型主要是半迟钝的煤,迟钝的煤, semilustrous 和有光泽的煤。半迟钝的煤和迟钝的煤的比例在盆边缘是更高的,特别在北边缘附近的区域。semilustrous 和有光泽的煤的数字向南并且向中央盆增加。这种状况显示不同形成煤的沼泽地环境让学生在煤部件上控制效果。另一观察是在 Ordos 煤序列,特别在更低的部分,一些沙岩床是厚的,直到有一种粗糙的谷物尺寸的 20 m。在 macerals 的更高的 fusinite 内容与更多的完全、常规的植物房间结构伴随一个更高的 semi-vitrinite 内容。fusinite 结构清楚、很好保存。在埋葬以后,房顶和地板岩石的岩性学能继续影响煤岩石学的进化。在房顶和地板的沙身体展出好物理条件以便毛孔水能维持氧化,循环和连接的一个长期的状态到煤。煤部件因此很长时间留在氧化环境。在盆中心,在埋葬和随后的煤床很快进入了减少的环境以后,相反地,开发的湖的外形和泥炭被 mudstone 很快盖住。作为结果,丰富的胶凝化作用发生了, vitrinite 内容增加了。Exinite 经常在煤床上在一个特定的位置积累了。尽管平均 exinite 内容不总体上高,它显著地确实贡献全部的烃产生。exinite 内容被低估了,特别非结晶的沥青的液体和它的重要性这里被强调。原因是液体容易流进有强壮的刚硬的 fusinite,或流进一些裂缝,在它通常被忽视的地方。 | Weihua Ao Wenhui Huang Chengmin Weng Xiuling Xiao Dameng Liu Xiuyi Tang Ping Chen Zhigen Zhao Huan Wan Robert B.Finkelman | 2012 | Geoscience Frontiers2012，3，1： | 3 |
| 15 | Wikstroelide M potently inhibits HIV replication by targeting reverse transcriptase and integrase nuclear translocation显示文摘AIM:To evaluate the anti-HIV activity and mechanism of action of wikstroelide M,a daphnane diterpene from Daphne acutiloba Rehder(Thymelaeaceae).METHOD:The anti-HIV activities of wikstroelide M against different HIV strains were evaluated by cytopathic effect assay and p24 quantification assay with ELISA.The inhibitory effect of wikstroelide M on HIV reverse transcription was analyzed by real-time PCR and ELISA.The effect of wikstroelide M on HIV-1 integrase nuclear translocation was observed with a cell-based imaging assay.The effect of wikstroelide M on LEDGF/p75-IN interaction was assayed by molecular docking.RESULTS:Wikstroelide M potently inhibited different HIV-1 strains,including HIV-1IIIB,HIV-1A17,and HIV-19495,induced a cytopathic effect,with EC50 values ranging from 3.81 to 15.65 ng·mL-1.Wikstroelide M also had high inhibitory activities against HIV-2ROD and HIV-2CBL-20-induced cytopathic effects with EC50 values of 18.88 and 31.90 ng·mL-1.The inhibitory activities of wikstroelide M on the three HIV-1 strains were further confirmed by p24 quantification assay,with EC50 values ranging from 15.16 to 35.57 ng·mL-1.Wikstroelide M also potently inhibited HIV-1IIIB induced cytolysis in MT-4 cells,with an EC50 value of 9.60 ng·mL-1.The mechanistic assay showed that wikstroelide M targeted HIV-1 reverse transcriptase and nuclear translocation of integrase through disrupting the interaction between integrase and LEDGF/p75.CONCLUSION:Wikstroelide M may be a potent HIV-1 and HIV-2 inhibitor,the mechanisms of action may include inhibition of reverse trascriptase activity and inhibition of integrase nuclear translocation through disrupting the interaction between integrase and LEDGF/p75. | ZHANG Xuan HUANG Sheng-Zhuo GU Wan-Gang YANG Liu-Meng CHEN Huan ZHENG Chang-Bo ZHAO You-Xing WAN David Chi-Cheong ZHENG Yong-Tang | 2014 | Chinese Journal of Natural Medicines2014，12，3： | 3 |
| 16 | cDNA cloning and expression pattern of two enolase genes from the Chinese oak silkworm, Antheraea pernyi显示文摘在这研究,二 enolase 基因从中国橡树蚕被孤立并且描绘, Antheraea perny,它被指定为 enolase 我和 II 分别地。enolase 我 cDNA 顺序是有编码 433 氨基酸的 1302 bp 的一个开的读物框架(ORF ) 的 1712 bp。enolase II cDNA 顺序是有编码 431 氨基酸的 1296 bp 的 ORF 的 1549 bp。二基因的氨基酸序列分享 enolase 的几个保存特征 / 地点。我显示出的 Antheraea pernyi enolase 93%97% 顺序身份到迄今为止可得到的 lepidopterans 的 enolases,到另外的无脊椎动物的 enolases 的 75%82% 身份,到包括脊椎动物的另外的有机体的 enolases 的 60%72% 身份,植物,和真菌。Antheraea pernyi enolase II 显示出 84% 身份到 Bombyx mori enolase II,而是 60% 身份到 A。pernyi enolase 我。在种系发生的树上, enolase II 从 A 定序。pernyi 和 B。mori 清楚地包括 A 与更高的有机体的 enolase 序列的多数被分开。pernyi 和 B。我定序的 mori enolase。由顺序比较和种系发生的分析,我们从 A 建议那 enolase II。pernyi 和 B。mori 可以是 enolase 总科的一个新成员。Antheraea pernyi enolase 我而 enolase II mRNA 在睾丸和卵巢明确地被表示, mRNA 在所有测试纸巾被发现,建议 enolase II 基因的产品可能与复制有关。抄本许多 A。pernyi enolase 我基因在冷吃惊以后并且显著地是显著地下面调整的在热吃惊以后起来调整,建议那 A。pernyi enolase 我基因可能由温度应力是可诱导的。 | Yanqun Liu Yuping Li Huan Wang Runxi Xia Xisheng Li Haolei Wan Li Qin Defu Jiang Cheng Lu Zhonghuai Xiang | 2010 | Acta Biochimica et Biophysica Sinica2010，42，11： | 2 |
| 17 | TEDA(Tianjin Economic-technological Development Area)——A General Survey, Experience and the Plan for Future Development.显示文摘Since its establishment about ten years ago, TEDA has taken advantage of eachopportune time created by China’s policy of opening wider and wider to the outsideworld and the rapid economic growth to deepen its reform and extend its exploitation,and thus has maintained a favorable momentum and an extraordinary high speed foreconomic development. With the total economic output of the whole area raised to | Tian gui-ming Chi Chang-gui Wan Qian Spokesman, Director of the Commission Office of TEDA. Visiting Professor of Tianjin Institute of Commerce M. A. of Economies, Vice Director of the Commission Office of TEDA Deputy Chief of Secretary Section of the Commission Office of TEDA Shen Huan | 1994 | 南开经济研究1994，，S1： | 2 |
| 18 | Optimal dosing time of Dachengqi decoction for protection of extrapancreatic organs in rats with experimental acute pancreatitis显示文摘BACKGROUND Acute pancreatitis(AP)is a pancreatic inflammatory disorder that is commonly complicated by extrapancreatic organ dysfunction.Dachengqi decoction(DCQD)has a potential role in protecting the extrapancreatic organs,but the optimal oral administration time remains unclear.AIM To screen the appropriate oral administration time of DCQD for the protection of extrapancreatic organs based on the pharmacokinetics and pharmacodynamics of AP rats.METHODS This study consisted of two parts.In the first part,24 rats were divided into a sham-operated group and three model groups.The four groups were intragastrically administered with DCQD(10 g/kg)at 4 h,4 h,12 h,and 24 h postoperatively,respectively.Tail vein blood was taken at nine time points after administration,and then the rats were euthanized and the extrapancreatic organ tissues were immediately collected.Finally,the concentrations of the major DCQD components in all samples were detected.In the second part,84 rats were divided into a sham-operated group,as well as 4 h,12 h,and 24 h treatment groups and corresponding control groups(4 h,12 h,and 24 h control groups).Rats in the treatment groups were intragastrically administered with DCQD(10 g/kg)at 4 h,12 h,and 24 h postoperatively,respectively,and rats in the control groups were administered with normal saline at the same time points.Then,six rats from each group were euthanized at 4 h and 24 h after administration.Serum amylase and inflammatory mediators,and pathological scores of extrapancreatic organ tissues were evaluated.RESULTS For part one,the pharmacokinetic parameters(C max,T max,T 1/2,and AUC 0→t)of the major DCQD components and the tissue distribution of most DCQD components were better when administering DCQD at the later(12 h and 24 h)time points.For part two,delayed administration of DCQD resulted in lower IL-6 and amylase levels and relatively higher IL-10 levels,and pathological injury of extrapancreatic organ tissues was slightly less at 4 h after administration,while the results were similar between the treatment and corresponding control groups at 24 h after administration.CONCLUSION Delayed administration of DCQD might reduce pancreatic exocrine secretions and ameliorate pathological injury in the extrapancreatic organs of AP rats,demonstrating that the late time is the optimal dosing time. | Jia-Qi Yao Lin Zhu Yi-Fan Miao Lv Zhu Huan Chen Ling Yuan Jing Hu Xiao-Lin Yi Qiu-Ting Wu Xi-Jing Yang Mei-Hua Wan Wen-Fu Tang | 2020 | World Journal of Gastroenterology2020，26，22： | 2 |
| 19 | Three dimensional nano-LiMn_(0.6)Fe_(0.4)PO_4@C/CNT as cathode materials for high-rate lithium-ion batteries显示文摘Three dimensional(3D) nano-LiMn_(0.6)Fe_(0.4)PO_4@C/CNT composite was successfully synthesized by an oleylamine-assisted solvothermal method. The prepared composite showed excellent electrochemical performance, especially superior high rate capability. It could deliver a specific discharge capacity of103.1 mAh/g, even at 80 C. The superior high rate performance of the as-prepared LiMn_(0.6)Fe_(0.4)PO_4@C/CNT electrode is attributed to its unique 3D conducting network:(1) the prepared LiMn_(0.6)Fe_(0.4)PO_4@C active particles were in nano-scale with a size of 30-50 nm;(2) LiMn_(0.6)Fe_(0.4)PO_4 nanoparticles were uniformly coated by amorphous carbon with a thickness of 3 nm;(3) the graphitized conductive CNTs were dispersed homogenously among the LiMn_(0.6)Fe_(0.4)PO_4@C active particles. The synergistic effect of the nanoscale amorphous carbon coated LiMn_(0.6)Fe_(0.4)PO_4@C active particles and the graphitized CNTs reduces the diffusion path of the lithium ions and benefits the transference ability of electron. | Huan Zhang Zhikai Wei Jinjin Jiang Lei Wang Qi Wan Tao Chen Meizhen Qu Mianzhong Chen | 2018 | Journal of Energy Chemistry2018，27，2： | 2 |
| 20 | An Expression for Gravity Generated by an Anomalous Geological Body and Its Application in Bathymetry Inversion显示文摘The gravity anomaly and vertical gravity gradient due to anomalous geological bodies are mainly computed by numerical methods,so it is difficult and time-consuming to use the gravity-geological method to invert seafloor topography.This paper addresses this issue by deriving an expression for gravity generated by a cylinder based on a series expansion.The choice of number for terms in the series is estimated by comparing with the numerical method,especially when the depth H=4000m,the accuracy of 1 mGal(1 Gal=10^(-2)m/s^(2))can be achieved when the series are 9.The expressions can be used to establish the relationships between the shape of an anomalous body and the generated vertical gravity and vertical gravity gradient,respectively.Finally,the potential applications of the expressions in inverting seafloor topography are illustrated by synthetic examples. | Huan XU Jinhai YU Xiaoyun WAN Lei LIANG | 2021 | Journal of Geodesy and Geoinformation Science2021，4，4： | 2 |
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