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| 1 | Mutation of the PHYTOENE DESATURASE 3 gene causes yellowish-white petals in Brassica napus显示文摘Oilseed rape (Brassica napus) with yellow flowers is an attractive ornamental landscape plant during the flowering period,and the development of different petal colors has become a breeding objective.Although yellowish flower color is a common variant observed in field-grown oilseed rape,the genetics behind this variation remains unclear.We obtained a yellowish-white flower (ywf) mutant from Zhongshuang 9 (ZS9) by ethyl methanesulfonate mutagenesis (EMS) treatment.Compared with ZS9,ywf exhibited a lower carotenoid content with a reduced and defective chromoplast ultrastructure in the petals.Genetic analysis revealed that the yellowish-white trait was controlled by a single recessive gene.Using bulked-segregant analysis sequencing (BSA-seq) and kompetitive allele-specific PCR(KASP),we performed map-based cloning of the ywf locus on chromosome A08 and found that ywf harbored a C-to-T substitution in the coding region,resulting in a premature translation termination.YWF,encoding phytoene desaturase 3 (PDS3),was highly expressed in oilseed rape petals and involved in carotenoid biosynthesis.Pathway enrichment analysis of the transcriptome profiles from ZS9 and ywf indicated the carotenoid biosynthesis pathway to be highly enriched.Further analyses of differentially expressed genes and carotenoid components revealed that the truncated Bna A08.PDS3 resulted in decreased carotenoid biosynthesis in the mutant.These results contribute to an understanding of the carotenoid biosynthesis pathway and manipulation of flower-color variation in B.napus. | Chuanji Zhao Luqman Bin Safdar Meili Xie Meijuan Shi Zhixue Dong Li Yang Xiaohui Cheng Yueying Liu Zetao Bai Yang Xiang Chaobo Tong Junyan Huang Lijiang Liu Shengyi Liu | 2021 | The Crop Journal2021，9，5： | 3 |
| 2 | Syntenic quantitative trait loci and genomic divergence for Sclerotinia resistance and ?owering time in Brassica napus显示文摘Oilseed rape(Brassica napus) is an allotetraploid with two subgenomes descended from a common ancestor. Accordingly, its genome contains syntenic regions with many duplicate genes, some of which may have retained their original functions, whereas others may have diverged. Here, we mapped quantitative trait loci(QTL) for stem rot resistance(SRR), a disease caused by the fungus Sclerotinia sclerotiorum, and flowering time(FT) in a recombinant inbred line population. The population was genotyped using B.napus 60 K single nucleotide polymorphism arrays and phenotyped in six(FT) and nine(SSR) experimental conditions or environments. In total, we detected 30 SRR QTL and 22 FT QTL and show that some of the major QTL associated with these two traits were co-localized,suggesting a genetic linkage between them. Two SRR QTL on chromosome A2 and two on chromosome C2 were shown to be syntenic, suggesting the functional conservation of these regions. We used the syntenic properties of the genomic regions to exclude genes for selection candidates responsible for QTL-associated traits. For example, 152 of the 185 genes could be excluded from a syntenic A2–C2 region. These findings will help to elucidate polyploid genomics in future studies, in addition to providing useful information for B. napus breeding programs. | Fengqi Zhang Junyan Huang Minqiang Tang Xiaohui Cheng Yueying Liu Chaobo Tong Jingyin Yu Tehrim Sadia Caihua Dong Lingyan Liu Baojun Tang Jianguo Chen Shengyi Liu | 2019 | Journal of Integrative Plant Biology2019，61，1： | 3 |
| 3 | pH-sensitive cationic guar gum/poly (acrylic acid) polyelectrolyte hydrogels: Swelling and in vitro drug release 显示文摘 | Yihong Huang Huiqun Yu Chaobo Xiao | 2007 | Carbohydrate Polymers2007，69，： | 1 |
| 4 | Fluorescence detection of Escherichia coli on mannose modified ZnTe quantum dots显示文摘Rapid detection and identification of Escherichia coli(E.coli)is essential to prevent its quickly spread.In this study,a novel fluorescence probe based on ZnTe quantum dots(QDs)modified by mannose(MAN)had been prepared for the determination of E.coli.The results showed that the obtained QDs showed excellent selectivity toward E.coli,and presented a good linearity in range of 1.0×10~5~1.0×10~8 CFU/mL.The optimum fluorescence intensity for detecting E.coli was found to be at pH 7.0 with a temperature of25℃and incubation time of 20 min.Under these optimum conditions,the detection limit of E.coli was4.6×10~4 CFU/mL.The quenching was discussed to be a static quenching procedure,which was proved by the quenching efficiency of QDs decreased with the temperature increasing. | Dudu Wu Dongming Wang Xiaomei Ye Kangrui Yuan Yuling Xie Baohong Li Chaobo Huang Tairong Kuang Zhiqiang Yu Zhi Chen | 2020 | Chinese Chemical Letters2020，31，6： | 1 |
| 5 | Electrospun cellulose acetate phthalate fibers for semen induced anti-HIV vaginal drug delivery显示文摘 | Chaobo Huang Stefaan J. Soenen Ellen van Gulck Guido Vanham Joanna Rejman Serge Van Calenbergh Chris Vervaet Tom Coenye Hans Verstraelen Marleen Temmerman Jo Demeester Stefaan C. De Smedt | 2011 | Biomaterials2011，，3： | 1 |
| 6 | pH-sensitive cationic guar gum/poly (acrylic acid) polyelectrolyte hydrogels:swelling and in vitro drug release显示文摘 | HUANG Yihong YU Huiqun XIAO Chaobo | 2007 | Carbohydrate Polymers2007，69，4： | 1 |
| 7 | High strength electrospun polymer nanofibers made from BPDA-PDA polyimide显示文摘 | Huang Chaobo Wang Suqing Zhang Hean | 2006 | European Polymer Journal2006，42，： | 1 |
| 8 | High strength electrospun polymer nanofibers made from BPDAPDA polyimide 显示文摘 | Huang Chaobo Wang Suqing Hou Haoqing | 2006 | European Polymer Journal2006，42，5： | 1 |
| 9 | High- strength mats from electrospun poly (p-phenylene biphenyhetracarboximide) nanofibers 显示文摘 | Huang Chaobo Chen Shuiliang Hou Haoqing | 2006 | Adv Mater2006，18，5： | 1 |
| 10 | High strength electrospun polymer nanofibers made from BPDA–PDA polyimide显示文摘 | Chaobo Huang Suqing Wang Hean Zhang Tingting Li Shuiliang Chen Chuilin Lai Haoqing Hou | 2005 | European Polymer Journal2005，，5： | 1 |
| 11 | High-strength mats from electrospun poly(p-phenylene biphenylteracarbox-imide)nanofibers显示文摘 | Huang Chaobo Chen Shuiliang Darrell H | 2006 | Advanced Materials2006，18，： | 1 |
| 12 | Mapping loci controlling fatty acid profiles, oil and protein content by genome-wide association study in Brassica napus显示文摘Optimizing the profile and quantity of fatty acids in rapeseed(Brassica napus L.) is critical for maximizing the value of edible oil and biodiesel. However, selection of these complex seed quality traits is difficult before haplotypes controlling their contents are identified. To efficiently identify genetic loci influencing these traits and underlying candidate genes and networks, we performed a genome-wide association study(GWAS) of eight seed quality traits(oil and protein content, palmitic, stearic, oleic, linoleic, eicosenoic and erucic acids content). The GWAS population comprised 370 diverse accessions, which were phenotyped in five environments and genotyped using 60K SNP arrays. The results indicated that oil and protein contents generally showed negative correlations, while fatty acid contents showed positive or negative correlations,with palmitic and erucic acid contents directly affecting oil content. Seven SNPs on five chromosomes were associated with both seed oil and protein content, and five genes orthologous to genes in Arabidopsis thaliana were predicted as candidates. From resequencing data, besides known haplotypes in Bna A.FAE1.a and Bna C.FAE1.a, three accessions harboring a new haplotype conferring moderate erucic acid content were identified. Interestingly, in a haplotype block, one haplotype was associated with high palmitic acid content and low oil content, while the others showed the reverse effects. This finding was consistent with a negative correlation between palmitic acid and oil contents, suggesting historical selection for high oil content. The identification by this study of genetic variation and complex correlations of eight seed quality traits may be beneficial for crop selection strategies. | Minqiang Tang Yuanyuan Zhang Yueying Liu Chaobo Tong Xiaohui Cheng Wei Zhu Zaiyun Li Junyan Huang Shengyi Liu | 2019 | The Crop Journal2019，7，2： | 1 |
| 13 | pH-sensitive cationic guar/poly (acrylic acid) polyelectrolyle hydrogels: swelling and in vitro drug release 显示文摘 | Yihong Huang Huiqun Yu Chaobo Xiao | 2007 | Carbohyd Polymers2007，69，： | 1 |
| 14 | High strength electrospun polymer nanofibers made from BPDA–PDA polyimide显示文摘 | Chaobo Huang Suqing Wang Hean Zhang Tingting Li Shuiliang Chen Chuilin Lai Haoqing Hou | 2005 | European Polymer Journal2005，，5： | 1 |
| 15 | Design of a novel mitochondria targetable turn-on fluorescence probe for hydrogen peroxide and its two-photon bioimaging applications显示文摘Considering that hydrogen peroxide(H2O2)plays significant roles in oxidative stress,the cellular signal transduction and essential biological process regulation,the detection and imaging of H2O2 in living systems undertakes critical responsibility.Herein,we have developed a novel two-photon fluorescence turn on probe,named as Pyp-B for mitochondria H2O2 detection in living systems.Selectivity studies show that probe Pyp-B exhibit highly sensitive response toward H2O2 than other reactive oxygen species(ROS)and reactive nitrogen species(RNS)as well as biologically relevant species.The fluorescence colocalization studies demonstrate that the probe can localize in the mitochondria solely.Furthermore,as a bio-compatibility molecule,the highly selective and sensitive of fluorescence probe Pyp-B have been confirmed by its cell imaging application of H2O2 in living A549 cells and zebrafishes under the physiological conditions. | Kangnan Wang Wen Ma Yuchun Xu Xin Liu Gui Chen Meng Yu Qiling Pan Chaobo Huang Xiaochuan Li Qingchun Mu Yongbing Sun Zhiqiang Yu | 2020 | Chinese Chemical Letters2020，31，12： | 1 |
| 16 | Thermal and mechanical properties of cationic guar gum/poly(acrylic acid) hydrogel membranes显示文摘 | Yihong Huang Jun Lu Chaobo Xiao | 2007 | Polymer Degradation and Stability2007，，： | 1 |
| 17 | Preparation and characterization of a quaternary ammonium derivative of konjac glucomannan显示文摘 | Huiqun Yu Yihong Huang Hou Ying Chaobo Xiao | 2006 | Carbohydrate Polymers2006，，1： | 1 |
| 18 | Thermal and mechanicalproperties of cationic guar gum/pely (acrylic acid) hydrogel membranes 显示文摘 | Yihong Huang Jun Lu Chaobo Xiao | 2007 | Polymer Degradation and Stability2007，92，2： | 1 |
| 19 | Electro- spun polymer nanofibres with small diameters 显示文摘 | Chaobo Huang Shuiliang Chen Chuilin Lai | 2006 | Nanotech- nology2006，17，6： | 1 |
| 20 | MoS_(2)nanosheets and bulk materials altered lipid profiles in 3D Caco-2 spheroids显示文摘MoS_(2)nanosheets(NSs)are novel 2D nanomaterials(NMs)with potential uses in many areas,and therefore oral exposure route to MoS_(2)NSs is plausible.Currently,MoS_(2)NSs are considered as biocompatible NMs,but there is lacking of systemic investigations to study the interactions of MoS_(2)NSs with intestinal cells.In this study,we exposed the 3D Caco-2 spheroids to MoS_(2)NSs or MoS_(2)powders(denoted as MoS_(2)-bulk),and investigated the potential adverse effects of MoS_(2)-materials based on transcriptomics and lipidomics analysis.As expected,both MoS_(2)NSs and MoS_(2)-bulk were dose-dependently internalized into 3D Caco-2 spheroids but did not induce cytotoxicity,membrane disruption or decrease of thiols.However,the Gene Ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)analysis indicated that nutrient absorption and metabolism was decreased.One of the most significantly decreased KEGG pathways is fat digestion and absorption(map04975),and Western blotting analysis further showed that fatty acid binding protein 1 and apolipoprotein A1,key proteins involved in fat digestion and absorption,were down-regulated by MoS_(2)NSs or MoS_(2)-bulk.In addition,BODIPY 493/503 staining suggested that exposure to MoS_(2)NSs and MoS_(2)-bulk decreased lipid levels in the spheroids.However,lipidomics data indicated that MoS_(2)materials only decreased 8 lipid classes,including lysophosphatidylcholine,lysodimethylphosphatidylethanolamine,N-acylethanolamine,ceramide phosphoethanolamines,gangliosides,lysosphingomyelin and sulfatide,whereas most of the lipid classes were indeed increased.In addition,MoS_(2)NSs was more potent to decrease the lipid classes compared with MoS_(2)-bulk.Combined,the results from this study showed that MoS_(2)NSs and bulk materials were non-cytotoxic but altered lipid profiles in 3D Caco-2 spheroids. | Min Xie Chaobo Huang Yongqi Liang Shuang Li Liping Sheng Yi Cao | 2022 | Chinese Chemical Letters2022，33，1： | 0 |
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