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5篇 您的检索式:作者名="Huanqing DONG"
    题名 作者 年代 出处 被引量
1Nitric oxide enhances the sensitivity of alpaca melanocytes to respond to α-melanocyte-stimulating hormone by up-regulating melanocortin-1 receptor显示文摘Yanjun Dong Jing Cao Haidong Wang Jie Zhang Zhiwei Zhu Rui Bai HuanQing Hao Xiaoyan He Ruiwen Fan Changsheng Dong 2010Biochemical and Biophysical Research Communications2010,,4:2
2A novel fuzzy classification entropy approach to image threshol- ding显示文摘Liu Dong Jiang Zhaohui Feng Huanqing 2006Pattern Recognition Letters2006,27,16:1
3HWM: a hybrid workload migration mechanism of metadata server cluster in data center显示文摘Jian LU Huanqing DONG Junwei ZHANG Zhenjun LIU Lu XU 2017Frontiers of Computer Science2017,11,1:1
4A novel fuzzy classification entropy approach to image thresholding显示文摘Liu Dong Jiang Zhaohui Feng Huanqing 2006Pattern Recognition Letters2006,27,7:1
5Characterization of two halophilic adenylate cyclases from Thermobifida halotolerans and Haloactinopolyspora alba显示文摘Adenylate cyclase(AC)is the key enzyme that catalyzes the formation of cAMP from ATP.In this study,we discovered two novel class Ⅲ ACs with a halophilic property from Thermobifida halotolerans DSM 44931(ThAC)and Haloactinopolyspora alba DSM 45211(HaAC),respectively.The recombinant ThAC and HaAC were expressed in Escherichia coli with molecular weights of 36.1 and 36.0 kDa respectively.The presence of 2500 and 2200 mmolL^(-1)1 NaCl significantly enhanced the enzyme activities of ThAC and HaAC,with 22-fold and 7.4-fold higher activities compared to those without NaCl,respectively.Several divalent metal ions were found to activate the recombinant ACs to different extents,and the optimal metal ion was Mg^(2+)for both ThAC and HaAC with concentrations of 80 mmol·L^(-1) and 40 mmol·L^(-1) respectively.Purified ThAC and HaAC had the optimal specific activities((4.59±0.35)×10^(4) and(7.76±0.52)×10^(4) U·mg^(-1))and catalytic efficiency(4.47 and 5.30 L·mmol^(-1)·s^(-1))at 45℃ and 40℃ respectively,while the optimum pH of both two recombinant ACs was 10.0.This is the first report of the halophilic Class III ACs,which could make new contributions to explore and study ACs for further associated investigations.Dahai Jiang Zhidi Min Jing Leng Huanqing Niu Yong Chen Dong Liu Chenjie Zhu Ming Li Wei Zhuang Hanjie Ying 2023Chinese Journal of Chemical Engineering2023,53,1:0
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