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| 1 | Genetic diversity of Tricholoma matsutake in Yunnan Province显示文摘To investigate the genetic diversity of Tricholoma matsutake,we studied ITS and IGS1 sequences and PCR polymorphism of a retrotransposon in 56 fruit bodies collected from 13 counties of 9 regions in Yunnan Province. We found one and three haplotypes based on ITS and IGS1 sequences,respectively. Moreover,there was no significant difference in PCR polymorphism of the retrotransposon among different populations. Compared with Jilin Province(China) and Japanese populations,although Yun-nan was highly homogenous to Japanese populations,low genetic diversity of T. matsutake in Yunnan did not support the view that this species originated from Yunnan. | SHA Tao ZHANG HanBo DING HuaSun LI ZongJu CHENG LiZhong ZHAO ZhiWei ZHANG YaPing | 2007 | Chinese Science Bulletin2007，52，9： | 8 |
| 2 | Isolation of Nanjing Local Strains of HHV-7 and Their Biological and Immunological Characteristics显示文摘To investigate the biological and immunological characteristics of the Nanjing local strains of HHV7, four strains of herpesvirus were isolated from saliva specimens of one healthy individual and three children suffering from a kidney disease in Nanjing. The viruses were identified by transmission electron microscopy(EM), indirect immunofluorescence assay (IFA) with a specific monoclonal antibody; nested polymerase chain reaction, restriction mapping and DNA sequencing. The virus-infected cells showed the typical cytophathic effect (CPE) under microscopy and could be detected by IFA with the human herpesvirus-7 (HHV-7) specific antibody. Under EM, herpesvirus-like and virions capsids could be found in their cytoplasm or nucleoplasm.HHV-7 DNA fragments amplified from infected cells by nested PCR were confirmed by restriction mapping and DNA sequencing. Similarly to DC strain, an known HHV-7 strain used in the present study as the positive control, the virus could be inactivated by ultraviolet irradiation for 10 min, heated at 45℃ for 30 min, pH<5 or>9 at 4℃ for 2 h and ether or chloroform for 10 h. The virus induced the production of TNF-α, IL-10 and IL-12p70 while inhibited IFN-γ secretion, increased the percentage of CD2+ cells while decreased that of CD4+ or CD45RA+ cells. The results indicate that the viruses isolated in Nanjing are HHV-7, which has similar biological characteristic to the known HHV-7 strain, DC. Infection with HHV-7 in vitro could affect immune function of lymphocytes by disturbing cytokine production and CD antigen expression. Cellular & Molecular Immunology. 2004; 1(5):367-372. | KunYao JieDou JianXu HuaSun GuangyongPeng QiangRen XiaohuiJi | 2004 | Cellular & Molecular Immunology2004，1，5： | 2 |
| 3 | Yielding potential of rubber(Hevea brasiliensis)in suboptimal environments显示文摘 | P riyadarshan P M Hoa T T T Huasun H | 2005 | J Crop Improvement2005，，14： | 1 |
| 4 | Yielding Potential of Rubber (Hevea brasiliensis) in Sub-Optimal Environments显示文摘 | P. M. Priyadarshan T. T. T. Hoa H. Huasun P. S. de Goncalves | 2005 | Journal of Crop Improvement (-)2005，，1： | 1 |
| 5 | Yielding potential of rubber (Hevea brasiliensis) in sub-optimal environments显示文摘 | Priyadarshan P M Hoa T T T Huasun H | 2005 | Journal of Crop Improvement2005，14，12： | 1 |
| 6 | Permian volcanism in the Mongolian orogenic zone, northeast China: geochemistry, magma sources and petrogenesis显示文摘 | Zhu Yongfeng Shi Huasun Li Binggu | 2001 | Cambridge University Press2001，138，2： | 1 |
| 7 | Comparative Study on Effects of Arnebia Euchroma (Royle) Johnst Granular and Decoction Forms on Medical AbortionwithMifepristone andMisoprostol显示文摘Objective To compare the effects of 2 dosage-forms (granular and decoction) of Arnebia euchroma (royle) Johnst (Arnebia EJ in short below) on medical abortion with that of mifepristone combined with misoprostol Methods Totally 648 women, who had pregnancy of 38-45 d and were willing to terminate pregnancy with mifepristone and misoprostol, were randomly divided into 3 groups, each of which was respectively given granular of Arnebia EJ, placebo granular, or decoction of Arnebia EJ besides mifepristone and misoprostol. The abortion results, bleeding duration, menstruation recovery and side-effects were observed. Results Neither complete abortion rates nor average bleeding durations of the granular group and the decoction group were significantly different (P>0.05). The complete abortion rate and bleeding duration of the two groups were respectively higher and shorter than those of the placebo group (P<0.05). However, the menstruation recov- ery was not significantly different among the three groups (P>0.05). The decoction of Arnebia EJ caused significantly more nausea and vomiting than the other groups (P<0.05). Conclusion The granular form did not have the odor of Arnebia EJ, and caused much less nausea and vomiting compared with the decoction form. The granular and decoc- tion forms were equally effective in improving the results of medical abortion. There- fore it is necessary to conduct further studies on the granular form of Arnebia EJ. | Jian-huaLIU HuaSUN Yun-yuFA | 2004 | Journal of Reproduction and Contraception2004，15，2： | 1 |
| 8 | Delayed induction and long term effects of mild hypothermia in afocal model of transient cerebral ischemia: neurological outcome and infract size 显示文摘 | Guo Huasun Kunis D | 2001 | J Neurosurgery2001，94，1： | 1 |
| 9 | Yielding Potential of Rubber(Hevea brasiliensis)in Sub--Optimal Environments显示文摘 | Priyadarshan P M Hoa T T T Huasun H | 2005 | J Crop Improv2005，14，12： | 1 |
| 10 | An Analysis of the Patents concerning Hevea brasiliensis显示文摘This paper mainly analyzes the application data of patents concerning Hevea brasiliensis in the world and China from the perspectives of time,research field,and the geographical distribution of applicants. It also interprets the patents for invention in China from time,research field,and types of applicants. Based on the study,it shows that although the patent applications fluctuate,the general trend is rising. And the patents are mostly concentrated in plant protection,and agricultural biotechnology. The Chinese applicants of patents for invention largely work in the research institutes,and most of these patents focus on the field of plant protection,agricultural biotechnology,and agro-processing.However,the foreign applicants mainly work in companies,and most of these patents only focus on plant protection. These findings will provide a reference for formulating development policies about natural rubber industry in China. | Xijuan LI Qing CHEN Bingjiang XU Xinxin REN Huasun HUANG Qiong LI | 2015 | Asian Agricultural Research2015，7，9： | 0 |
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