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| 1 | Structural Analyses of Short-Chain Prenyltransfemses Identify an Evolutionarily Conserved GFPPS Clade in Brassicaceae Plants显示文摘Terpenoids 是专业化植物的代谢物的最大、很多样的类,它在植物开发期间在多样的生理的进程工作。在植物 terpenoids 的生合成,短链的 prenyltransferases (SC 磅) 和萜烯 synthases (TPS ) ,在决定 terpenoid 的 playcritical 角色差异。有不同的链长度的 SC 磅 biosynthesize prenyl 焦磷酸盐,和这些混合物是 terpenoids 的直接先锋。Arabidopsis thaliana 拥有其功能清楚地不被知道的 SC 磅的亚群。在这研究,我们集中于 10 geranylgeranyl 焦磷酸盐象 synthase 一样[GGPPSL ] 蛋白质,它通常被认为生产 GGPP [C20 ] 。我们发现 Arabidopsis GGPPSL 基因家庭的成员的一个子集经历了 neo-functionalization:GGPPSL6, 7, 9,和 10 主要举办 geranylfarnesyl 焦磷酸盐 synthase 活动(C25;renamedAtGFPPS1, 2, 3,和 4 ) ,并且 GGPPSL8 生产甚至更长的链 prenyl 焦磷酸盐(≥C30;renamedpolyprenyl 焦磷酸盐 synthase 2, AtPPPS2 ) 。由解决 AtGFPPS2, AtPPPS2,和 AtGGPPS11 的水晶结构,我们揭示 SC 磅的产品链长度决心机制并且作为 “three floors” 解释它当模特儿。用这个模型,我们识别了在 Brassicaceae 植物散布并且发现 GFPPS 基因典型地与编码 TPS 的基因一起发生的新奇 GFPPS clade,形成 GFPPS-TPS 基因簇。 | ChengyuanWang Qingwen Chen Dongjie Fan Jianxu Li Guodong Wang Peng Zhang | 2016 | Molecular Plant2016，9，2： | 19 |
| 2 | Signal pathways involved in emodin-induced contraction of smooth muscle cells from rat colon显示文摘AIM: To investigate the effects induced by emodin on single smooth muscle cells from rat colon in vitro, and to determine the signal pathways involved.METHODS: Cells were isolated from the muscle layers of Wistar rat colon by enzymatic digestion. Cell length was measured by computerized image micrometry. Intracellular Ca^2+ ([Ca^2+]i) signals were studied using the fluorescent Ca^2+ indicator fluo-3 and confocal microscopy. PKCα distribution at rest state or after stimulation was measured with immunofluorescence confocal microscopy.RESULTS: (1) Emodin dose-dependently caused colonic smooth muscle cells contraction, (2) emodin induced an increase in intracellular Ca^2+ concentration; (3) the contractile responses induced by emodin were respectively inhibited by preincubation of the cells with ML-7 (an inhibitor of MLCK) and calphostin C (an inhibitor of PKC), (4) Incubation of cells with emodin caused translocation of PKCα from cytosolic area to the membrane.CONCLUSION: Emodin has a direct contractile effect on colonic smooth muscle cell. This signal cascade induced by emodin is initiated by increased [Ca^2+]i and PKCα translocation,which in turn lead to the activation of MLCK and the suppression of MLCP. Both of them contribute to the emodininduced contraction. | TaoMa Qing-HuiQi JianXu Zuo-LiangDong Wen-XiuYang | 2004 | World Journal of Gastroenterology2004，10，10： | 11 |
| 3 | Heavy metal(loid) pollution in mine wastes of a Carlin-type gold mine in southwestern Guizhou, China and its environmental impacts显示文摘Mercury pollution resulting from artisanal gold mining is a serious environmental problem in many developing countries. In this study, we analyzed heavy metal(loid) contamination in mine wastes from a Carlin-type gold mine in southwestern Guizhou, China. Highly elevated levels of As, Hg, Tl, Sb, and Cd—up to 5844, 28,29, 581 and 3.0 mg/kg, respectively—were observed in the mine wastes, but no significant accumulation of Cr, Ni, Cu,Zn, and Pb was found. The smelting process of gold ores had significant impacts on the enrichment of As, Tl, and Hg in the mine wastes. Due to the significant metal(loid)pollution in this gold mine, the ecological risks are classified as ‘‘very high.'' Hg and As are the major pollutants with a relative contribution of 55.9 % and 24.2 % to the risk index, respectively. Phytoremediation could be used to reduce heavy metal contamination and recycle the gold simultaneously. Hg–As–Tl pollution should be considered in gold extraction at Carlin-type gold mines. | Ping Li Junfang Zhang Jianxu Wang Zhonggen Li | 2015 | Chinese Journal Of Geochemistry2015，34，3： | 9 |
| 4 | Contractile effects and intracellular Ca^(2+)signalling induced by emodin in circular smooth muscle cells of rat colon显示文摘AIM: To investigate whether emodin has any effects on circular smooth muscle cells of rat colon and to examine the mechanism underlying its effect.METHODS: Smooth muscle cells were isolated from the circular muscle layer of Wistar rat colon and the cell length was measured by computerized image micrometry.Intracellular Ca2+([Ca2+]i) signalling was studied in smooth muscle cells using Ca2+ indicator Fluo-3 AM on a laserscanning confocal microscope.RESULTS: Emodin dose-dependently induced smooth muscle cells contraction. The contractile responses induced by emodin were inhibited by preincubation of the cells with ML-7 (an inhibitor of MLCK). Emodin caused a large, transient increase in [Ca2+]i followed by a sustained elevation in [Ca2+]i.The emodin -induced increase in [Ca2+]i was unaffected by nifedipine, a voltage-gated Ca2+-channel antagonist, and the sustained phase of the rising of [Ca2+]i was attenuated by extracellular Ca2+ removal with EGTA solution. Inhibiting Ca2+release from ryanodine-sensitive intracellular stores by ryanodine reduced the peak increasein [ca2+]i.Using heparin, an antagonist of IP3R, almost abolished the peak increase in [Ca2+]i.CONCLUSION: Emodin has a direct excitatory effect on circular smooth muscle cells in rat colon mediated via Ca2+/CaM dependent pathways. Furthermore, emodin-induced peak [Ca2+]i increase may be attributable to the Ca2+release from IP3 sensitive stores, which further promote Ca2+ release from ryanodine-sensitive stores through CICR mechanism.Additionally, Ca2+ influx from extracellular medium contributes to the sustained increase in[Ca2+]i. | TaoMa Qing-HuiQi Wen-XiuYang JianXu Zuo-LiangDong | 2003 | World Journal of Gastroenterology2003，9，8： | 7 |
| 5 | Diterpenoid UDP-Glycosyltransferases from Chinese Sweet Tea and Ashitaba Complete the Biosynthesis of Rubusoside显示文摘 | Yuwei Sun Zhuo Chen Jianxu Li Jianhua Li Huajun LV Jingya Yang Weiwei Li Dingan Xie Zhiqiang Xiong Peng Zhang Yong Wang | 2018 | Molecular Plant2018，11，10： | 7 |
| 6 | Male panda (Ailuropoda melanoleuca) urine contains kinship information显示文摘Chemical communication plays an important role in kin selection and mate choice in mammals. The covariance of odor-genes of rodents has been documented and kinship odor has been proposed and termed, yet little is known of the relationship between genetic relatedness and chemical composition of kinship odors. Giant pandas (Ailuropoda melanoleuca) rely substantially on chemical communication to mediate their social interactions. To examine the relationship between genetic relatedness and compounds in the urine/anogenital gland secretions, we compared the similarities between genetic relatedness and the chemical profiles of anogenital gland secretions and urine via lineage construction and GC-MS (gas chromatography and mass spectrometry). We found that information about kinship odors was present only in the urine of male adults in the mating season but absent in the non-mating season. Adult females and all sub-adults did not have such kinship odors in either mating or non-mating season. Therefore, kinship odor in the panda was contingent on age, sex, and season. This is the first report about the condition-dependent expression of kinship odor, which may have a sig- nificant implication in the practice of panda conservation in relation to chemical communication and sexual selection. | LIU DingZhen WEI RongPing ZHANG GuiQuan YUAN Hong WANG ZhiPeng SUN Lixing ZHANG Jianxu ZHANG HeMin | 2008 | Chinese Science Bulletin2008，53，18： | 6 |
| 7 | Design and Analysis of a Multi-Legged Robot with Pitch Adjustive Units显示文摘The paper proposes a novel multi-legged robot with pitch adjustive units aiming at obstacle surmounting.With only 6 degrees of freedom,the robot with 16 mechanical legs walks steadily and surmounts the obstacles on the complex terrain.The leg unit with adjustive pitch provides a large workspace and empowers the legs to climb up obstacles in large sizes,which enhances the obstacle surmounting capability.The pitch adjustment in leg unit requires as few independent adjusting actuators as possible.Based on the kinematic analysis of the mechanical leg,the biped and quadruped leg units with adjustive pitch are analyzed and compared.The configuration of the robot is designed to obtain a compact structure and pragmatic performance.The uncertainty of the obstacle size and position in the surmounting process is taken into consideration and the parameters of the adjustments and the feasible strategies for obstacle surmounting are presented.Then the 3D virtual model and the robot prototype are built and the multi-body dynamic simulations and prototype experiments are carried out.The results from the simulations and the experiments show that the robot possesses good obstacle surmounting capabilities. | Qiang Ruan Jianxu Wu Yan-an Yao | 2021 | Chinese Journal of Mechanical Engineering2021，34，3： | 5 |
| 8 | Multi-functional polyethersulfone nanofibrous membranes with ultra-high adsorption capacity and ultra-fast removal rates for dyes and bacteria显示文摘The adsorption technology has been widely applied in water remediation for contamination removal of dyes and bacteria,by virtue of the advantages of adsorption technology including high efficiency,energy conservation and ease of operation.Simultaneous removal of dyes and bacteria has been realized by some reported materials,but to achieve satisfactory adsorption amounts and rates remain an unmet goal for decades.Herein,a poly(methacrylatoethyl trimethyl ammonium chloride-co-methyl methacrylate)copolymer was synthesized,and then blended with polyethersulfone for the fabrication of nanofibrous membranes via electrospinning for the use of fast and massive removal of dyes and bacteria.Owing to the introduction of abundant quaternary ammonium groups,the maximum adsorption amount for methyl orange was up to 909.8 mg g^(-1).In addition,the modified nanofibrous membranes showed good recyclability,broad applications in severe environments,selective adsorption ability,and excellent dynamic removal performance.Especially,thanks to the abundant functional groups,the membranes showed fast adsorption ability for bacteria through electrostatic interaction.It should be noted that the clearance ratio for Staphylococcus aureus or Escherichia coli by 6 min of static adsorption could reach 93%or 90%for each.Additionally,dynamic removal ratio via filtration with the nanofibrous membranes could reach 99.7%for Staphylococcus aureus or 98.7%for Escherichia coli in 90 s.Therefore,the proposed approach towards the quaternary ammonium modified polyethersulfone nanofibrous membranes creates a new route for ultra-high adsorption capacity and ultra-fast removal rates for dyes and bacteria in water remediation. | Jianxu Bao Hang Li Yuanting Xu Shengqiu Chen Zhoujun Wang Chunji Jiang Huilin Li Zhiwei Wei Shudong Sun Weifeng Zhao Changsheng Zhao | 2021 | Journal of Materials Science & Technology2021，，19： | 4 |
| 9 | Microbionic and peristaltic robots in a pipe显示文摘A microbionic and peristaltic robot that simulates the motion of an earthworm to move within a micropipe is proposed. The robot consists of three flexible units. Each unit is composed of two plates connected with three shape memory alloys (SMA) 120° apart, the rubber gasbag around the SMA wires inflated with air inside. Each unit corresponds to a segment of an earthworm, and the SMA and rubber gasbag have the same functions as the cricoid and longitudinal muscles of the earthworm. A control system is designed to fulfill the control of the three flexible units motions, such as stretching, shrinking and bending, so the microrobot can walk forward and backward, and choose the direction. | MA Jianxu LIU Xiang MA Jianhua LI Mingdong MA Peisun | 2000 | Chinese Science Bulletin2000，45，11： | 4 |
| 10 | Novel application of bergapten and quercetin with anti-bacterial,osteogenesis-potentiating,and anti-inflammation tri-effects显示文摘The bacteria-mediated inflammatory conditions adversely affect the osseointegration process of endosseous implants,which can even lead to implant malfunction or failure.Local drug delivery has been designed to exert anti-inflammatory and antibacterial activities,but whether this strategy has an effect on the compromised osseointegration under inflammation has rarely been studied.The present study focused on the osteoinductive efficacy of two known phytoestrogens[bergapten(BP)and quercetin(QE)]on implant sites under multiple bacteria-infected conditions in situ.Furthermore,the gene expression profiles of rat bone mesenchymal stem cells(rBMSCs)treated with BP and QE in the presence of Porphyromonas gingivalis-derived lipopolysaccharide were identified.The results showed that both drugs,especially QE,had significant potentiating effects on promoting osteogenic differentiation of rBMSCs,resisting multiple pathogens,and reducing inflammatory activity.Meanwhile,RNA sequencing analysis highlighted the enriched gene ontology terms and the differentially expressed genes(Vps25,Il1r2,Csf3,Efemp1,and Ccl20)that might play essential roles in regulating the above tri-effects,which provided the basis for the drug delivery system to be used as a novel therapeutic strategy for integrating peri-implant health.Overall,our study confirmed that QE appeared to outperform BP in osteogenesis and bacterial killing but not in anti-inflammation.Moreover,both drugs possess favorable tri-effects and can serve as the pivotal agents for the drug delivery system to boost osseointegration at inflammatory implant sites. | Jianxu Wei Xiaomeng Zhang Yuan Li Xinxin Ding Yi Zhang Xue Jiang Hongchang Lai Junyu Shi | 2021 | Acta Biochimica et Biophysica Sinica2021，53，6： | 4 |
| 11 | Locating Malleable Bulk Metallic Glasses in Zr-Ti-Cu-Al Alloys with Calorimetric Glass Transition Temperature as an Indicator显示文摘We defined the plastic deformability under constrained loading conditions as malleability for bulk metallic glass (BMG) materials. Quaternary Zr-Ti-Cu-Al alloys in the Zr-rich composition range are selected to investigate the compositional dependence of malleability assessed by bending testing and glass transition temperature (Tg ). As indicated, increasing the Al or Cu concentration in the alloys leads to the rise of T g . The Zr(61)Ti2Cu(25)Al(12) (ZT1) and Zr(61.6)Ti(4.4)Cu(24)Al(10) (ZT3) alloys exhibit an optimal combination of lower T g and higher glass-forming ability. The malleable BMGs such as ZT1 manifests two characters during deformation, the stable propagation of a single shear band indicated by large shear offsets and easy proliferation of shear bands. With increasing the T g of BMG, the yield strength σy,Young's modulus and shear modulus simultaneously increase as well, while the Poisson s ratio decreases. The σy of ZT1 BMG is about 1680 MPa in compression and 1600 MPa in tension. In tensile loading, no any visible plasticity appears even when the strain rate increases up to the order of magnitude of 10(-1)s(-1). In consistent with the T g , malleability of Zr-Ti-Cu-Al BMGs manifests significant compositional dependence. The malleable BMG is associated with lower Tg , as well as lower shear modulus or higher Poisson s ratio, which can be understood on the basis of the correlation of Tg with shear energy barrier in metallic glass. Thus, the calorimetric Tg can be used as an indicator to screen malleable BMG-forming composition, with advantage of experimental accessibility. | Qiang He Jian Xu jianxu@imr.ac.cn | 2012 | Journal of Materials Science & Technology2012，28，12： | 4 |
| 12 | STOCHASTIC RESONANCE MEASURED BY THEMUTUAL INFORMATION IN THE NEURONSTHAT TRANSMIT CHAOTIC SPIKE TRAINS显示文摘The spike trains generated by a neuron model are studied by the methods of nonlinear time series analysis. The results show that the spike trains are chaotic. To investigate effect of noise on transmission of chaotic spike trains, this chaotic spike trains are used as a discrete subthreshold input signal to the integrate-and-fire neuronal model and the FitzHugh-Nagumo(FHN) neuronal model working in noisy environment. The mutual information between the input spike trains and the output spike trains is calculated, the result shows that the transformation of information encoded by the chaotic spike trains is optimized by some level of noise, and stochastic resonance(SR) measured by mutual information is a property available for neurons to transmit chaotic spike trains. | Gong Pulin Xu Jianxue Hu Sanjue(Institution of Nonlinear dynamics, School of Civil Engineering and Mechanics,Xi’an Jiaotong University, Xi’an 710049, China)(Department of Physiology, Fourth Military Medical University, Xi’an, 710032, China) | 1999 | 生物物理学报1999，15，4： | 3 |
| 13 | Three-dimensional Construction and Omni-directional Rolling Analysis of a Novel Frame-like Lattice Modular Robot显示文摘Lattice modular robots possess diversity actuation methods, such as electric telescopic rod, gear rack, magnet, robot arm, etc. The researches on lattice modular robots mainly focus on their hardware descriptions and reconfiguration algorithms. Meanwhile, their design architectures and actuation methods perform slow telescopic and moving speeds, relative low actuation force verse weight ratio, and without internal space to carry objects. To improve the mechanical performance and reveal the locomotion and reconfiguration binary essences of the lattice modular robots, a novel cube-shaped, frame-like, pneumatic-based reconfigurable robot module called pneumatic expandable cube(PE-Cube) is proposed. The three-dimensional(3D) expanding construction and omni-directional rolling analysis of the constructed robots are the main focuses. The PE-Cube with three degrees of freedom(Do Fs) is assembled by replacing the twelve edges of a cube with pneumatic cylinders. The proposed symmetric construction condition makes the constructed robots possess the same properties in each supporting state, and a binary control strategy cooperated with binary actuator(pneumatic cylinder) is directly adopted to control the PE-Cube. Taking an eight PE-Cube modules' construction as example, its dynamic rolling simulation, static rolling condition, and turning gait are illustrated and discussed. To testify telescopic synchronization, respond speed, locomotion feasibility, and repeatability and reliability of hardware system, an experimental pneumatic-based robotic system is built and the rolling and turning experiments of the eight PE-Cube modules' construction are carried out. As an extension, the locomotion feasibility of a thirty-two PE-Cube modules' construction is analyzed and proved, including dynamic rolling simulation, static rolling condition, and dynamic analysis in free tipping process. The proposed PE-Cube module, construction method, and locomotion analysis enrich the family of the lattice modular robot and provide the instruction to design the lattice modular robot. | DING Wan WU Jianxu YAO Yan'an | 2015 | Chinese Journal of Mechanical Engineering2015，28，4： | 3 |
| 14 | Structural Insights into the CatalyticMechanism of a Plant Diterpene Glycosyltransferase SrUGT76G1显示文摘Diterpene glycosyltransferase UGT76G1 from Stevia rebaudiana (SrUGT76G1) is key to the generation ofeconomically important steviol glycosides (SGs), a group of natural sweeteners with high-intensity sweetness. SrUGT76G1 accommodates a wide range of steviol-derived substrates and many other small molecules. We report here the crystal structures of SrUGT76G1 in complex with multiple ligands to answer howthis enzyme recognizes diterpenoid aglycones and catalyzes the 1,3-sugar chain branching. A spaciouspocket for sugar-acceptor binding was observed from the determined SrUGT76G1 structures, which canexplain its broad substrate spectrum. Residues Gly87 and Leu204 lining the pocket play key roles in switching between diterpenoid and flavonoid glucosylation. An engineered mutant of SrUGT76G1, T284S, couldcatalyze a selectively increased production of next-generation sweetener rebaudioside M, with diminishedside product of rebaudioside I. Taken together, these resutls provide significant insights into molecularbasis of the substrate specificity of scarcely documented diterpenoid glycosyltransferases and wouldfacilitate the structure-guided glycoengineering to produce diversified diterpenoids with new activities. | Zhifeng Liu Jianxu Li Yuwei Sun Peng Zhang Yong Wang | 2020 | Plant Communications2020，1，1： | 3 |
| 15 | Crystal structure of plant PLDα1 reveals catalytic and regulatory mechanisms of eukaryotic phospholipase D显示文摘Phospholipase D(PLD)hydrolyzes the phosphodiester bond of glycerophospholipids and produces phosphatidic acid(PA),which acts as a second messenger in many living organisms.A large number of PLDs have been identified in eukaryotes,and are viewed as promising targets for drug design because these enzymes are known to be tightly regulated and to function in the pathophysiology of many human diseases.However,the underlying molecular mechanisms of catalysis and regulation of eukaryotic PLD remain elusive.Here,we determined the crystal structure of full-length plant PLDα1 in the apo state and in complex with PA.The structure shows that the N-terminal C2 domain hydrophobically interacts with the C-terminal catalytic domain that features two HKD motifs.Our analysis reveals the catalytic site,substrate-binding mechanism,and a new Ca2+-binding site that is required for the activation of PLD.In addition,we tested several efficient small-molecule inhibitors against PLDα1,and suggested a possible competitive inhibition mechanism according to structure-based docking analysis.This study explains many long-standing questions about PLDs and provides structural insights into PLD-targeted inhibitor/drug design. | Jianxu Li Fang Yu Hui Guo Renxue Xiong Wenjing Zhang Fangyuan He Minhua Zhang Peng Zhang | 2020 | Cell Research2020，30，1： | 3 |
| 16 | A Fast Calculation Method for Long-term Security-constrained Unit Commitment of Large-scale Power Systems with Renewable Energy显示文摘With the increase in the penetration rate of renewable energy, the planning and operation of power systems will face huge challenges. To ensure the sufficient utilization of renewable energy, the reasonable arrangement for the long-term power generation plan has become more crucial. Security-constrained unit commitment(SCUC) is a critical technical means to optimize the long-term power generation plan. However, the plentiful power sources and the complex grid structure in largescale power systems will bring great difficulties to long-term SCUC. In this paper, we propose a fast calculation method for long-term SCUC of large-scale power systems with renewable energy. First, a method for unit status reduction based on temporal decomposition is proposed, which will reduce plenty of binary variables and intertemporal constraints in SCUC. Then,an efficient redundant constraint identification(RCI) method is developed to reduce the number of network constraints. Furthermore, a joint accelerated calculation framework for status reduction and RCI is formed, which can reduce the complexity of long-term SCUC while ensuring a high-precision feasible solution. In case studies, numerical results based on two test systems ROTS2017 and NREL-118 are analyzed, which verify the effectiveness and scalability of the proposed calculation method. | Qian Yang Jianxue Wang Hongrui Yin Qingtao Li | 2022 | Journal of Modern Power Systems and Clean Energy2022，10，5： | 3 |
| 17 | Decentralized Demand Management Based on Alternating Direction Method of Multipliers Algorithm for Industrial Park with CHP Units and Thermal Storage显示文摘This paper proposes a decentralized demand management approach to reduce the energy bill of industrial park and improve its economic gains.A demand management model for industrial park considering the integrated demand response of combined heat and power(CHP)units and thermal storage is firstly proposed.Specifically,by increasing the electricity outputs of CHP units during peak-load periods,not only the peak demand charge but also the energy charge can be reduced.The thermal storage can efficiently utilize the waste heat provided by CHP units and further increase the flexibility of CHP units.The heat dissipation of thermal storage,thermal delay effect,and heat losses of heat pipelines are considered for ensuring reliable solutions to the industrial park.The proposed model is formulated as a multi-period alternating current(AC)optimal power flow problem via the second-order conic programming formulation.The alternating direction method of multipliers(ADMM)algorithm is used to compute the proposed demand management model in a distributed manner,which can protect private data of all participants while achieving solutions with high quality.Numerical case studies validate the effectiveness of the proposed demand management approach in reducing peak demand charge,and the performance of the ADMM-based decentralized computation algorithm in deriving the same optimal results of demand management as the centralized approach is also validated. | Jingdong Wei Yao Zhang Jianxue Wang Lei Wu Peiqi Zhao Zhengting Jiang | 2022 | Journal of Modern Power Systems and Clean Energy2022，10，1： | 3 |
| 18 | Effects of salinity on embryonic and larval development of Chinese mitten crab Eriocheir sinensis(Decapoda: Brachyura) and salinity-induced physiological changes显示文摘To investigate the effects of salinity on early development of Chinese mitten crab ( Eriocheir sinensis ), and the salinity tolerance mechanism of embryos, different developmental stages of embryos (gastrula, eyespot and pre-hatching stage), and hatched stage I zoea and megalopa, were exposed to a range of salinities (1, 5, 10, 15 (control), 20, 25, 30, 35 and 40). Hatching, survival and molting were monitored. Effects of 24-hour hypersaline (35) and hyposaline (1) stress on egg diameter, water content, Na +/K +-ATPase (NKA) activity, and crustacean hyperglycemic hormone (CHH) gene mRNA expression in embryos and megalopa, are reported. Embryos are more tolerant of low (≤ 5) than high (≥25) salinities, with optimum ranges for gastrula and pre-hatching stage embryos being 5-20, and for eyespot embryo and stage I zoea, 10-20. Most megalopa can molt to the first juvenile instar by day 5 at salinities between 1 and 40, whereas molting of megalopa stages was delayed at 40. Hypersaline conditions resulted in a loss of moisture, reduction of egg volume, and a signifi cant increase in NKA activity and CHH mRNA expression at some developmental stages. Hyposaline conditions did not affect moisture content or egg volume, but resulted in decreased NKA activity and CHH mRNA expression in embryos. For megalopa stages, NKA activity was significantly upregulated following both hypo- and hypersaline stress. Our results suggest high salinity will inhibit development and hatching of E. sinensis embryos, and low salinity will affect the survival of their stage I zoea. Increased NKA activity in embryos and megalopa stages might indicate a hyporegulation response under hypersaline conditions. These findings provide useful information for spawning ground protection of indigenous E . sinensis and enrich the knowledge of embryonic tolerance mechanisms of hyperregulating crustaceans following osmotic stress. | WANG Ruifang HUANG Xiaorong WANG Haihua LU Jianxue SHI Xiaotao FENG Guangpeng ZHUANG Ping | 2019 | Journal of Oceanology and Limnology2019，37，5： | 2 |
| 19 | Adaptive neural control for pure-feedback nonlinear time-delay systems with unknown dead-zone: a Lyapunov-Razumikhin method显示文摘This paper addresses the problem of adaptive neural control for a class of uncertain pure-feedback nonlinear systems with multiple unknown state time-varying delays and unknown dead-zone. Based on a novel combination of the Razumikhin functional method, the backstepping technique and the neural network parameterization, an adaptive neural control scheme is developed for such systems. All closed-loop signals are shown to be semiglobally uniformly ultimately bounded, and the tracking error remains in a small neighborhood of the origin. Finally, a simulation example is given to demonstrate the effectiveness of the proposed control schemes. | Zhaoxu YU Jianxu LUO Ji LIU | 2013 | 控制理论与应用(英文版)2013，11，1： | 2 |
| 20 | Model-free Demand Response Scheduling Strategy for Virtual Power Plants Considering Risk Attitude of Consumers显示文摘Driven by modern advanced information and communication technologies,distributed energy resources have great potential for energy supply within the framework of the virtual power plant(VPP).Meanwhile,demand response(DR)is becoming increasingly important for enhancing the VPP operation and mitigating the risks associated with the fluctuation of renewable energy resources(RESs).In this paper,we propose an incentivebased DR program for the VPP to minimize the deviation penalty from participating in the power market.The Markov decision process(MDP)with unknown transition probability is constructed from the VPP’s prospective to formulate an incentivebased DR program,in which the randomness of consumer behavior and RES generation are taken into consideration.Furthermore,a value function of prospect theory(PT)is developed to characterize consumer’s risk attitude and describe the psychological factors.A model-free deep reinforcement learning(DRL)-based approach is proposed to deal with the randomness existing in the model and adaptively determine the optimal DR pricing strategy for the VPP,without requiring any system model information.Finally,the results of cases tested demonstrate the effectiveness of the proposed approach. | Yi Kuang Xiuli Wang Hongyang Zhao Tao Qian Nailiang Li Jianxue Wang Xifan Wang | 2023 | CSEE Journal of Power and Energy Systems2023，9，2： | 2 |
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