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| 1 | Cd uptake in rice cultivars treated with organic acids and EDTA显示文摘A pot experiment was conducted to examine the activity of antioxidant enzymes, the content of malondialdehyde (MDA), proline and protein, and Cd uptake in different rice cultivars exposed to Cd (0, 1 and 5 mg/kg) in the presence of organic acids and ethylenediamine tetraacetic acid (EDTA). The results showed the increase in activity of dismutase (SOD), contents of proline and protein but a decline in activities of peroxidase (POD) and catalase (CAT), and MDA content for cultivars Xiushui63 and IIyou527. The resistance to Cd was higher in Xiushui63 than that in IIyou527 under the same Cd treatment. Cadmium contents in grain, straw and roots of both cultivars were markedly reduced in the presence of organic acids and EDTA. Grain Cd contents was the highest for plants treated with organic acids, followed by organic acids + 1/2EDTA, and the lowest with EDTA; Cd contents in straw and root were the lowest for plants treated with organic acids, followed by organic acids + 1/2EDTA, and the highest with EDTA treatment when exposed to Cd. | Weihong Xu Yangrui Li Jianping He Qifu Ma Xiaojing Zhang Guiqing Chen Huixian Wang Haibo Zhang | 2010 | Journal of Environmental Sciences2010，22，3： | 9 |
| 2 | Influence of leptin on luteinizing hormone and follicle stimulating hormone secreted from cultured rat anterior pituitary cells显示文摘BACKGROUND: Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE: To detect the effect of different dosages of leptin on luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion from in vitro cultured rat anterior pituitary cells. DESIGN: Contrast study based on cells. SETTING: This study was performed in the Basic Institute of Chengde Medical College, Chengde City, Hebei Province, China from March to June 2007. MATERIALS: Eighteen female Wistar rats of three months of age, weighing 200-220 g, and of clean grade were used. Leptin was provided by Peprotech Company, DMEM culture medium by Invitrogen Company, and the radioimmunological kit by Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. METHODS: Three glandular organs were regarded as one group for culture of anterior pituitary cells. In the control group, saline was added to the culture medium instead of leptin. In the leptin group, leptin was prepared into different concentrations of 1×10-12, 1×10-11, 1×10-9, 1×10-7, and 1×10-6 mol/L for stimulation of cultured cells. The culture supernatant was obtained at three hours after additional of saline/leptin. MAIN OUTCOME MEASURES: Contents of LH and FSH were detected by radioimmunology. RESULTS: Following leptin stimulation, LH release increased with increasing concentrations of leptin up to 1×10-9 mol/L, where LH release peaked. LH release then progressively decreased with increasing leptin concentrations (P < 0.01). LH release in the leptin (1×10-12, 1×10-11, 1×10-7, and 1×10-6 mol/L) groups was significantly higher than that in the control group (P < 0.01). FSH content in the leptin (1×10-11, 1×10-9, and 1×10-7 mol/L) groups was significantly higher than that in the control group (P < 0.01). CONCLUSION: Leptin can directly affect pituitary tissue to promote the secretion of LH and FSH in a dose-dependent manner. | Yuebing Qiao Xiuyan Ma Huixian Cui | 2008 | Neural Regeneration Research2008，3，6： | 2 |
| 3 | hiPSC-derived NSCs effectively promote the functional recovery of acute spinal cord injury in mice显示文摘Spinal cord injury(SCI)is a common disease that results in motor and sensory disorders and even lifelong paralysis..The transplantation of stem cells,such as embryonic stem cells(ESCs),induced pluripotent stem cells(iPSCs),mesenchymal stem cells(MSCs),or subsequently generated stem/progenitor cells,was predicted to be a promising treatment for SCI.In this study,we aimed to investigate effect of human iPSC-derived neural stem cells(hiPSC-NSCs)and umbilical cord-derived MSCs(huMSCs)in a mouse model of acute SCI.Acute SCI mice model was established and were randomly treated as phosphate-buffered saline(PBS)(control group),repaired with 1×10^(5)hiPSC-NSCs(NSC group),and 1×10^(5)huMSCs(MSC group),respectively,in a total of 54 mice.Hind limb motor function was evaluated in open-field tests using the Basso Mouse Scale(BMS)at days post operation(dpo)1,3,5,and 7 after spinal cord injury,and weekly thereafter.Spinal cord and serum samples were harvested at dpo 7,14,and 21. | Ma Jun Kong Desheng Cui Huixian | 2021 | 解剖学杂志2021，44，S01： | 1 |
| 4 | Transplanted iPSC-derived neural stem cells for spinal cord injury显示文摘Spinal cord injury(SCI),one of the most devastating central nervous system(CNS)disorders,can lead to severe neurological disabilities,the most severe forms of which include persistent paraplegia or quadriplegia.The efficacy of induced pluripotent stem cell(iPSC)derived neural stem cells(NSC)transplantation on functional recovery following acute spinal cord injury(SCI)was detected.NSC derived from iPSC was characterized by expression of specific markers(SOX2,nestin and PAX6). | Kong Desheng Feng Baofeng Guo Ruigyun Ernest Asiamah He Jingjing Zhang Shuhan Lyu Fei Liu Xin Ma Jun Huixian Cui | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 5 | Testosterone improves spatial cognitive dysfunction via androgen non-genomic effeet in Tfm mice显示文摘The non-genomic actions of androgen on cognitive function have been extensively studied.However,the underlying mechanisms remain controversial.In this study,we used testicular feminized(Tfm)mouse as a cell research model,which had androgen receptor deficiency and low circulating testosterone level,to investigate whether testosterone improves spatial cognitive dysfunction via its androgen non-genomic actions.We found that testosterone supplementation can improve the spatial cognitive function,hippocampal neuron danage and dendritic spine density in Tfm mice,but no statistical difference was found in fear memory function. | Chen Meiqin Li Sha Zhang Yizhou Mi Shixiong Du Juan Wang Chang Ma Min Cui Huixian | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 6 | Redox-dependent regulation of end-binding protein 1 activity by glutathionylation显示文摘Cytoskeletal proteins are susceptible to glutathionylation under oxidizing conditions,and oxidative damage has been implicated in several neurodegenerative diseases.End-binding protein 1(EB1)is a master regulator of microtubule plus-end tracking proteins(+TIPs)and is critically involved in the control of microtubule dynamics and cellular processes.However,the impact of glutathionylation on EB1 functions remains unknown.Here we reveal that glutathionylation is important for controlling EB1 activity and protecting EB1 from irreversible oxidation.In vitro biochemical and cellular assays reveal that EB1 is glutathionylated.Diamide,a mild oxidizing reagent,reduces EB1 comet number and length in cells,indicating the impairment of microtubule dynamics.Three cysteine residues of EB1 are glutathionylated,with mutations of these three cysteines to serines attenuating microtubule dynamics but buffering diamide-induced decrease in microtubule dynamics.In addition,glutaredoxin 1(Grx1)deglutathionylates EB1,and Grx1 depletion suppresses microtubule dynamics and leads to defects in cell division orientation and cell migration,suggesting a critical role of Grx1-mediated deglutathionylation in maintaining EB1 activity.Collectively,these data reveal that EB1 glutathionylation is an important protective mechanism for the regulation of microtubule dynamics and microtubule-based cellular activities. | Miao Chen Jian Wang Yang Yang Tao Zhong Peng Zhou Huixian Ma Jingrui Li Dengwen Li Jun Zhou Songbo Xie Min Liu | 2021 | Science China(Life Sciences)2021，64，4： | 0 |
| 7 | Fragmentation of brain apolipoprotein E(ApoE)and its relevance in late onset Alzheimer's disease显示文摘Late-onset Alzheimer's disease(LOAD)is the most common cause of dementia in the elderly.Polymorphism in apolipoprotein E(ApoE)gene(ε2,ε3,and ε4)is the greatest LOAD risk factor.The translation products are ApoE2,ApoE3,and ApoE4,respectively.ApoE fragments are present in senile plaques and neurofibillary tangles but their contributions to AD pathogenesis are not clear. | Ernest Asiamah Jingjing He Ruiyun Guo Baofeng Feng Desheng Kong Xin Liu Jun Ma Huixian Cui | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 8 | NuMA forms condensates through phase separation to drive spindle pole assembly显示文摘Dear Editor,Chromosome movement in mitosis is orchestrated by a microtubule-based protein machinery called the mitotic spindle(Hyman and Karsenti,1996;Compton,2000).The bipolar organization of the mitotic spindle is essential for the accurate segregation of chromosomes into daughter cells.Defects in bipolar spindle assembly can cause chromosome instability and aneuploidy,which are frequently observed in malignant tumors(Silkworth et al.,2009;McGranahan et al.,2012).The nuclear mitotic apparatus(NuMA)is a protein critical for bipolar spindle organization primarily due to its functions in the formation of spindle poles.Dysregulation of NuMA has been reported in a number of cancer types and is associated with cancer development(Bruning-Richardson et al.,2012).Therefore,it is of great significance to understand the molecular mechanisms by which NuMA contributes to spindle pole assembly. | Huixian Ma Feifei Qi Li Ji Songbo Xie Jie Ran Min Liu Jinmin Gao Jun Zhou | 2022 | Journal of Molecular Cell Biology2022，14，1： | 0 |
| 9 | THE CHEMICAL MODIFICATION OF E. Coli L-ASPARAGINASE WITH O - CARBOXYMETHYLATED CHITOSAN显示文摘Escherichia colt L - asparaginase was modified with O - carboxymethylated chitosan using glutaraldehyde as a coupling agent. The resulting coujugate retained more than 50% of its original enzyme activity under theprotection of its normal substrate or product and shoWed marked resistance toproteolysis by trypsin and chymotrypsin. | Li Minqin Ma JianbiaoPan Zaiqun Xu Huixian Wang Daobin He Binglin(The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation,Institute of Polymer Chemistry, Nankai University, Tianjin 300071, P. R. China) | 1998 | Chinese Journal of Reactive Polymers1998，7，1： | 0 |
| 10 | Clinical efficacy and safety of mesenchymal stem cells in patients with diabetes mellitus显示文摘Diabetes mellitus(DM)as a chronic metabolic disease is threatening human health seriously.The aim of this study was to systematically review the evidence from RCTs and conduct comprehensive assessment of therapeutic efficacy and safety of mesenchymal stem cells(MSCs)in DM.PubMed,Web of Science,Ovid,the Cochrane Library and CNKI were searched until April 30,2020.Seven RCTs were eligible,including 413 participants. | He Jingjing Kong Desheng Asiamah Ernest Am ponsah Guo Ruiyun Feng Baofeng Ma Jun Timothy O'Brien Cui Huixian | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 11 | Generation of a human iPSC line from a sporadic amyotrophic lateral sclerosis patient and motor neuron differentiation显示文摘Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disease that affects motor neurons in the central nervous system,which leads to muscle.atrophy and progressive paralysis.W hile mutations in several genes responsible for familial ALS have been identified,the pathogenesis of sporadic ALS,which accounts for approximately 90%of all ALS cases,remains unclear. | Feng Baofeng Asiamah Ernest Am ponsah Guo Ruiyun Kong Desheng He Jingjing Ma Jun Cui Huixian | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 12 | Establishment of amyotrophic lateral sclerosis induced pluripotent stem cells and neuronal differentiation显示文摘Amyotrophic lateral sclerosis(ALS)is a motor neuron disease that seriously threatens the health of patients.The mechanism is unclear.Most ALS disease models cannot accurately reflect the pathological characteristics of ALS.Induced pluripotent stem cells(iPSC)can best mimic lesions and is used to reveal the mechanism of ALS and develop therapeutic drugs.In the process of iPSC reprogramming,the selection of somatic cells and the optimization of conditions are critical to the progress of iPSC culture.Skin biopsies were used in this study to optimize the isolation and culture method of fibroblasts,and established a optimized somatic cell culture process with high tissue utilization rate,short culture period and high success rate. | Guo Ruiyun Feng Baofeng Kong Desheng Ernest Asiamah He Jingjing Lv Fei Ma Jun Huixian Cui | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 13 | Boosting electrocatalytic selectivity in carbon dioxide reduction:The fundamental role of dispersing gold nanoparticles on silicon nanowires显示文摘Carbon dioxide electrochemical reduction(CO_(2)RR)has been recognized as an efficient way to mitigate CO_(2)emissions and alleviate the pressure on global warming and associated environmental consequences.Gold(Au)is reported as stable and active electrocatalysts to convert CO_(2)to CO at low overpotential due to its moderate adsorption strength of^(*)COOH and^(*)CO.The request for improved catalytic performance,however,is motivated by current unsatisfied catalytic selectivity because of the side hydrogen evolution reaction.In this context,the design of Au based binary catalysts that can boost CO selectivity is of great interest.In the present work,we report that Au nanoparticles can be feasibly dispersed and anchored on silicon nanowires to form Au-Si binary nanomaterials.The Au-Si may stably drive CO_(2)RR with a CO Faraday efficiency of 95.6%at−0.6 V vs.RHE in 0.5 mol/L KHCO_(3)solution.Such selectivity outperforms Au particles by up to 61%.Controlled experiments illustrate that such catalytic enhancement can chiefly be ascribed to electronic effects of binary catalysts.Theoretical calculations reveal that spontaneously produced silicon oxide may not only inhibit hydrogen evolution reaction,but also stabilize the key intermediate^(*)COOH in CO formation. | Fan Liao Xing Fan Huixian Shi Qing Li Mengjie Ma Wenxiang Zhu Haiping Lin Youyong Li Mingwang Shao | 2022 | Chinese Chemical Letters2022，33，9： | 0 |
| 14 | Exploration of Hanshi Zufei prescription for treatment of COVID-19 based on network pharmacology显示文摘Objective:Network pharmacology combines drug and disease targets with biological information networks based on the integrity and systematicness of the interactions between drugs and disease targets.This study aims to explore the molecular basis of Hanshi Zufei formula for treatment of COVID-19 based on network pharmacology and molecular docking techniques.Methods:Using TCMSP,the chemical constituents and molecular targets of Atractylodis Rhizoma,Citri Reticulatae Pericarpium,Magnoliae Officinalis Cortex,Pogostemonis Herba,Tsaoko Fructus,Ephedrae Herba,Notopterygii Rhizoma et Radix,Zingiberis Rhizoma Recens,and Arecae Semen were investigated.The predicted targets of novel coronavirus were screened using the NCBI and Gene Cards databases.To further screen the drug-disease core targets network,the corresponding target proteins were queried using multiple databases(Biogrid,DIP,and HPRD),a protein interaction network graph was constructed,and the network topology was analyzed.The molecular docking studies were also performed between the network’s top 15 compounds and the coronavirus(SARS-Co V-2)3 CL hydrolytic enzyme and angiotensin conversion enzymeⅡ(ACE2).Results:The herb-active ingredient-target network contained nine drugs,86 compounds,and 49 drugdisease targets.Gene ontology(GO)enrichment analysis resulted in 1566 GO items(P<0.05),among which 1438 were biological process items,35 were cell composition items,and 93 were molecular function items.Fourteen signal pathways were obtained by enrichment screening of the KEGG pathway database(P<0.05).The molecular docking results showed that the affinity of the core active compounds with the SARS-CoV-23 CL hydrolase was better than for the other compounds.Conclusion:Several core compounds can regulate multiple signaling pathways by binding with 3 CL hydrolase and ACE2,which might contribute to the treatment of COVID-19. | Xinrui Li Zishuai Wen Mingdong Si Yuxin Jia Huixian Liu Yuguang Zheng Donglai Ma | 2022 | Chinese Herbal Medicines2022，14，2： | 0 |
| 15 | Rapid actions of androgen on PSD95 expression mediated by Ca^(2+)/CaMKI pathway in HT22 cells显示文摘The rapid actions of androgen on synaptic plasticity have been extensively studied.However,the underlying mechanisms remain controversial.In this study,we used mouse hippocampal neuron cell line HT22 cells as a cell research model to investigate whether Ca^(2+)/CaMKII mediates androgen rapid actions on synaptic plasticity,especially for synaptic protein PSD95 expression.Using calcium imaging,we verified incubation with 100 nmol/L testosterone for 20 min had the most significant effect on intracellular Ca^(2+)level.In addition,the depolarization of resting membrane potential after testosterone stimulation was observed by patch clamp.We found that the depolarization of resting membrane potential was to-40 mV. | Mi Shixiong Li Sha Zhang Yizhou Chen Meiqin Du J uan Wang Chang Ma Min Cui Huixian | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 16 | Protective effect of testosterone on endogenous apoptosis induced by Aβ42 in HT22 cells显示文摘Aβhas attracted a great deal of interest in the field of Alzheimer'diseases(AD)research,and its influence on the cells and the neuronal system,has been a pressing issue to clarify with a goal of diagnostic and therapeutic development.In our study,we determine whether mitochondrial impairment by Aβis fatal enough to cause neuronal death in AD conditions.Using Western blot to detect the expression of Bax and Bel-2,we verified incubatina HT22cells with 10 nmol/L Aβfor 24 h had the significant effect on cell apoptosis. | Muhammad Abbas Zhang Yizhou Li Sha Chen Meiqin Mi Shixiong Du Juan Wang Chang Ma Min Cui Huixian | 2021 | 解剖学杂志2021，44，S01： | 0 |
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