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| 1 | Computational pharmacological studies on cardiovascular disease by Qishen Yiqi Diwan显示文摘Computational pharmacological methods were used to study the distribution of 1729 compounds contained in a Chinese medicine,Qishen Yiqi Diwan,in chemical space.The results show that most of these compounds have good drug-like properties.Molecular docking was used to study the interactions between 1729 compounds of Qishen Yiqi Diwan and 26 drug targets related to cardiovascular disease and the distribution of 1729 compounds in drug-target space.The results may shed light on the action mechanism and the search for the active compounds in Qishen Yiqi Diwan. | GU JiangYong YUAN Gu ZHU YongHong XU XiaoJie | 2009 | Science China Chemistry2009，52，11： | 9 |
| 2 | Finite element methods on the boundaryproblem of constant differential equations 显示文摘 | GU Jiangyong | 2004 | Journalof Gansu Education College: Natural Science Edition2004，18，4： | 1 |
| 3 | Effect of curcumin on nitric oxide synthase expression in lipopolysaccharide-activated microglia cells and the anti-oxidative effect of curcumin显示文摘BACKGROUND: It has been demonstrated that curcumin can increase the activities of various anti-oxidase in blood and tissue, effectively eliminate various free radicals, reduce the production of peroxisome, and alleviate oxidative stress reaction. Whether it has the same effect on microglia? OBJECTIVE: To observe the effects of curcumin on the expressions of inducible nitric oxide synthase (iNOS), nuclear factor-κB (NF-κB), and superoxide dismutase (SOD) in microglial cell line BV stimulated by lipopolysaccharide (LPS). DESIGN: An observational comparative study. SETTING: Research Room of Biochemistry, Medical College of Nantong University. MATERIALS: Mice microglia cell line BV, iNOS and NF-κB reporter gene plasmids were presented by Dr. Bhat.NR. from the Medical University of South Carolina (USA). Curcumin was produced by the Xi'an Branch of China Chengdu Scholar Bio-Tech. Co.,Ltd.; LPS (E.Coli O26:B6), anti-mice iNOS monoclonal antibody, horseradish peroxidase labeled goat-anti-mice IgG were the products of Sigma Company (USA). METHODS: The experiments were carried out in the Research Room of Biochemistry, Medical College of Nantong University from May 2006 to April 2007. ① Detection of iNOS: The cells were seeded onto 24-well plate at the density of 1×105, After the cells had adhered to the cover glasses, the cells were grouped as negative control group (the primary antibody was replaced by phosphate buffered solution PBS); normal control group (the cells were normally cultured); LPS-treated group (the cells were treated with LPS for 24 hours); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours). The expressions of iNOS protein were detected with immunocytochemical staining. ② Determination of iNOS and NF-κB gene activities: According to the introduction of the kit for transfection, iNOS or NF-κB report gene plasmids were transiently transfected with LipofectamineTM2000 liposomes into the cells in the 24-well plate for 24 hours. The cells were divided into normal control group (the cells were normally cultured after transfected with report gene plasmids); blank plasmid group (the cells were normally cultured after transfected with blank plasmids); LPS-treated group (the cells were treated with LPS for 4 hours after transfected with report gene plasmids); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours after transfected with report gene plasmids). The content of luciferase in the cell lysis buffer was determined after cell lysis. ③ Determination of SOD activity: The cells were seeded into culture bottle at the density of 1×106, and the divided into four groups, including normal control group (the cells were normally cultured); LPS-treated group (the cells were treated with LPS for 24 hours); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours); vitamin C+LPS group (the cells were treated with vitamin C for 1 hour and LPS for 24 hours). The SOD activity was determined with xanthine oxidase and quantitative colorimetric assay. MAIN OUTCOME MEASURES: The expressions of iNOS protein, iNOS and NF-κB, and the activity of SOD were observed. RESULTS: ① Expression of iNOS protein in microglia: The expression of iNOS protein in the LPS-treated group was obviously higher than that in the negative control group (P < 0.01); Those in the curcumin+LPS group were significantly decreased as compared with that in the LPS-treated group (P < 0.01). ② Expressions of iNOS and NF-κB genes: The expressions of iNOS and NF-κB genes in the LPS-treated group were significantly higher than those in the normal control group (P < 0.01); Those in the curcumin+LPS group were significantly lower than those in the LPS-treated group (P < 0.01). ③ SOD activity: The activity of SOD in the LPS-treated group was significantly lower than those in the normal control group (P < 0.01). It in the curcumin+LPS group and vitamin C +LPS group was significantly higher than that in the LPS-treated group (P < 0.01). CONCLUSION: Curcumin could inhibit the expression of iNOS in the activated microglia, and it also has the abilities in eliminating free radicals and antagonizing lipid peroxidation. | Jianlan Gu Kaiyan Yang Jiangyong Yang Jianhua Shi | 2007 | Neural Regeneration Research2007，2，12： | 1 |
| 4 | FREPD: A Robust Federated Learning Framework on Variational Autoencoder显示文摘Federated learning is an ideal solution to the limitation of not preser-ving the users’privacy information in edge computing.In federated learning,the cloud aggregates local model updates from the devices to generate a global model.To protect devices’privacy,the cloud is designed to have no visibility into how these updates are generated,making detecting and defending malicious model updates a challenging task.Unlike existing works that struggle to tolerate adversarial attacks,the paper manages to exclude malicious updates from the glo-bal model’s aggregation.This paper focuses on Byzantine attack and backdoor attack in the federated learning setting.We propose a federated learning frame-work,which we call Federated Reconstruction Error Probability Distribution(FREPD).FREPD uses a VAE model to compute updates’reconstruction errors.Updates with higher reconstruction errors than the average reconstruction error are deemed as malicious updates and removed.Meanwhile,we apply the Kolmogorov-Smirnov test to choose a proper probability distribution function and tune its parameters to fit the distribution of reconstruction errors from observed benign updates.We then use the distribution function to estimate the probability that an unseen reconstruction error belongs to the benign reconstruction error distribution.Based on the probability,we classify the model updates as benign or malicious.Only benign updates are used to aggregate the global model.FREPD is tested with extensive experiments on independent and identically distributed(IID)and non-IID federated benchmarks,showing a competitive performance over existing aggregation methods under Byzantine attack and backdoor attack. | Zhipin Gu Liangzhong He Peiyan Li Peng Sun Jiangyong Shi Yuexiang Yang | 2021 | Computer Systems Science & Engineering2021，39，12： | 0 |
| 5 | Computational methods and applications for quantitative systems pharmacology显示文摘Background: Quantitative systems pharmacology (QSP) is an emerging discipline that integrates diverse data to quantitatively explore the interactions between drugs and multi-scale systems including small compounds, nucleic acids, proteins, pathways, cells, organs and disease processes. Results: Various computational methods such as ADME/T evaluation, molecular modeling, logical modeling, network modeling, pathway analysis, multi-scale systems pharmacology platforms and virtual patient for QSP have been developed. We reviewed the major progresses and broad applications in medical guidance, drug discovery and exploration of pharmacodynamic material basis and mechanism of traditional Chinese medicine. Conclusion: QSP has significant achievements in recent years and is a promising approach for quantitative evaluation of drug efficacy and systematic exploration of mechanisms of action of drugs. | Fuda Xie Jiangyong Gu | 2019 | Frontiers of Electrical and Electronic Engineering in China2019，7，1： | 0 |
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