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| 1 | A microfluidic biosensor for rapid detection of Salmonella typhimurium based on magnetic separation,enzymatic catalysis and electrochemical impedance analysis显示文摘Rapid screening of foodborne pathogens is of great significance to ensure food safety.A microfluidic biosensor based on immunomagnetic separation,enzyme catalysis and electrochemical impedance analysis was developed for rapid and sensitive detection of S.typhimurium.First,the bacterial sample,the magnetic nanoparticles(MNPs)modified with capture antibodies,and the enzymatic probes modified with detection antibodies and glucose oxidase(GOx)were simultaneously injected into the microfluidic chip,followed by mixing and incubation to form MNP-bacteria-probe sandwich complexes.Then,glucose with high impedance was injected into the chip and catalyzed by the GOx on the complexes into hydrogen peroxide with high impedance and gluconic acid with low impedance,which was finally measured using the low-cost interdigitated microelectrode and the electrochemical impedance analyzer to determine the target bacteria.Under the optimal conditions,this biosensor could quantitatively detect S.typhimurium at the concentrations from 1.6×10^(2) CFU/m L to 1.6×10^(6) CFU/m L in 1 h with the low detection limit of 73 CFU/m L.Besides,this biosensor was demonstrated with good feasibility for practical applications by detecting the S.typhimurium spiked chicken meat samples. | Yingjia Liu Dong Jiang Siyuan Wang Gaozhe Cai Li Xue Yanbin Li Ming Liao Jianhan Lin | 2022 | Chinese Chemical Letters2022，33，6： | 3 |
| 2 | Recent progress in porous organic polymers and their application for CO_(2) capture显示文摘Carbon capture,storage,and utilization(CCSU)is recognized as an effective method to reduce the excessive emission of CO_(2).Absorption by amine aqueous solutions is considered highly efficient for CO_(2) capture from the flue gas because of the large CO_(2) capture capacity and high selectivity.However,it is often limited by the equipment corrosion and the high desorption energy consumption,and adsorption of CO_(2) using solid adsorbents has been receiving more attention in recent years due to its simplicity and high efficiency.More recently,a great number of porous organic polymers(POPs)have been designed and constructed for CO_(2) capture,and they are proven promising solid adsorbents for CO_(2) capture due to their high Brunauer-Emmett-Teller(BET)surface area(SBET),adjustable pore size and easy functionalization.In particular,they usually have rigid skeleton,permanent porosity,and good physiochemical stability.In this review,we have a detailed review for the different POPs developed in recent years,not only the design strategy,but also the special structure for CO_(2) capture.The outlook of the opportunities and challenges of the POPs is also proposed. | Jiajia Wang Lizhi Wang You Wang Du Zhang Qin Xiao Jianhan Huang You-Nian Liu | 2022 | Chinese Journal of Chemical Engineering2022，35，2： | 2 |
| 3 | Administration of B7-H3 targeted chimeric antigen receptor-T cells induce regression of glioblastoma显示文摘Dear Editor,Nowadays,glioblastoma(GBM)was the most common and lethal form of primary intracranial tumor.Despite standard-of-care therapy,GBM still exhibited a poor prognosis with 5-years survival rate less than 5%.Recent years,adoptive CAR-T therapy came to be a novel immunotherapy in treating malignant tumors.Great progress has been made by CD19 targeted CAR-T cells against refractory B cell cancers.Recent studies also reported about the clinical potential of CAR-T therapy targeting IL13Ra2 and EGFRvlll in treating GBM.1,2 However,limited numbers of therapeutic targets in GBM may preclude it from progress and being popularized.B7-H3(CD276)has been found to be overexpressed by many tumors and tumor-infiltrating dendritic cell.3 Our previous studies suggested the potent anti-tumor effect of B7-H3 targeted CAR-T cells against GBM in preclinical models. | Xin Tang Yuelong Wang Jianhan Huang Zongliang Zhang Fujun Liu Jianguo Xu Gang Guo Wei Wang Aiping Tong Liangxue Zhou | 2021 | Signal Transduction and Targeted Therapy2021，6，4： | 1 |
| 4 | Tertiary amino groups modi?ed macroporous crosslinked poly(styrene-co-divinylbenzene)and its oxidized adsorbent:Synthesis,characterization,and adsorption behavior显示文摘 | Huang Jianhan Huang Kelong Liu Suqin | 2009 | Journal of Hazardous Materials2009，162，： | 1 |
| 5 | Characterization and high- pressure microfluidization-induced activation of polyphenoloxidase from chinese pear显示文摘 | LIU Wei LIU Jianhan XIE Mingyong | 2009 | Journal of Agricultural and Food Chemistry2009，57，12： | 1 |
| 6 | Colloids and Surfaces A: Physicoehemical and Engineering Aspects 显示文摘 | Huang Jianhan Huang Kelong Liu Suqin Wang A Ting Yan Chen | 2008 | 330(1): 55-612008，330，1： | 1 |
| 7 | Adsorption properties of tea polyphenols onto three polymeric adsorbents with amide group显示文摘 | Jianhan Huang Kelong Huang Suqin Liu Qiong Luo Mancai Xu | 2007 | Journal of Colloid And Interface Science2007，，2： | 1 |
| 8 | Development and performance test of a laser controlled land levelingmachine 显示文摘 | Lin Jianhan Liu Gang Wang Maohua | 2007 | Agricultural Mechanization in Asia Africa and Latin America2007，38，2： | 1 |
| 9 | A lab-on-a-disc platform based on nickel nanowire net and smartphone imaging for rapid and automatic detection of foodborne bacteria显示文摘Foodborne pathogenic bacteria have been considered as a major risk factor for food safety. It is of great significance to carry out in-field screening of pathogenic bacteria to prevent the outbreaks of foodborne diseases. In this study, a portable lab-on-a-disc platform with a microfluidic disc was developed for rapid and automatic detection of Salmonella typhimurium using a nickel nanowire(Ni NW) net for effective separation of target bacteria, horseradish peroxidase nanoflowers(HRP NFs) for efficient amplification of biological signals, and a self-developed smartphone APP for accurate analysis of colorimetric images. First,the microfluidic disc was preloaded with reagents and samples and centrifuged to form one bacterial sample column, one immune Ni NW column, one HRP NF column, two washing buffer columns and one tetramethylbenzidine(TMB) column, which were separated by air gaps. Then, a rotatable magnetic field was specifically developed to assemble the Ni NWs into a net, which was automatically controlled by a stepped motor to successively pass through the sample column for specific capture of target bacteria, the HRP NF column for specific label of target bacteria, the washing columns for effective removal of sample background and non-specific binding NFs, and the TMB column for colorimetric determination of target bacteria. The color change of TMB from colorless to blue was finally analyzed using the smartphone APP to quantitatively determine the target bacteria. This lab-on-a-disc platform could detect Salmonella typhimurium from 5.6 × 10^(1) CFU/20 μL to 5.6 × 10^(5) CFU/20 μL in 1 h with a lower detection limit of 56 CFU/20 μL. The recovery of target bacteria in spiked chicken samples ranged from 97.5% to 101.8%. This portable platform integrating separation, labeling, washing, catalysis and detection onto a single disc is featured with automatic operation, fast reaction, and small size and has shown its potential for in-field detection of foodborne pathogens. | Xiaoting Huo Lei Wang Wuzhen Qi Na Rong Yingjia Liu Siyuan Wang Hong Duan Jianhan Lin | 2022 | Chinese Chemical Letters2022，33，4： | 0 |
| 10 | Research trends of worldwide ophthalmologic randomized controlled trials in the 21st century:A bibliometric study显示文摘Background:Randomized controlled trials(RCTs)are often considered the gold standard and the cornerstone for clinical practice.However,bibliometric studies on worldwide RCTs of ophthalmology published in the 21st century have not been reported in detail yet.This study aims to perform a bibliometric study and visualization analysis of worldwide ophthalmologic RCTs in the 21st century.Methods:Global ophthalmologic RCTs from 2000 to 2022 were searched in the Web of Science Core Collection.The number of publications,country/region,institution,author,journal,and research hotspots of RCTs were analyzed using HistCite,VOSviewer,CiteSpace,and Excel software.Results:2366 institutions and 90 journals from 83 countries/regions participated in the publication of 1769 global ophthalmologic RCTs,with the United States leading in the number of volumes and research field,and the Moorfields Eye Hospital contributing to the most publications.Ophthalmology received the greatest number of publications and co-citations.Jeffrey S.Heier owned the most publications and Jost B.Jonas owned the most cocitations.The knowledge foundations of global ophthalmologic RCTs were mainly retinopathy,glaucoma,dry eye disease(DED),and cataracts,and anti-vascular endothelial growth factor(VEGF)therapy(ranibizumab),topical ocular hypotensive medication,laser trabeculoplasty.Anti-VEGF therapy for age-related macular degeneration(AMD),DME(diabetic macular edema),and DED,the use of new diagnostic tools,and myopia were the hottest research highlights.Anti-VEGF therapy,prompt laser,triamcinolone,and verteporfin photodynamic therapy for AMD,DME,and CNV(choroidal neovascularization),DED,myopia,and open-angle glaucoma were the research hotspots with the longest duration.The future research hotspots might be DED and the prevention and control of myopia.Conclusions:Overall,the number of global ophthalmologic RCTs in the 21st century was keeping growing,there was an imbalance between the regions and institutions,and more efforts are required to raise the quantity,quality,and global impact of high-quality clinical evidence in developing countries/regions. | Hao Wang Qiang Ye Weihe Xu Jing Wang Jianhan Liu Xintong Xu Wenfang Zhang | 2023 | Advances in Ophthalmology Practice and Research2023，3，4： | 0 |
| 11 | Study on Application of Evaluation System to Quinoa显示文摘The study of crop varieties has developed from qualitative research to quantitative research at a deeper level.Analysis and evaluation is a commonly used quantitative research method,and the evaluation index system and its weight are the basis of the evaluation system,which plays an important role in the scientificity of the evaluation results.In this paper,quinoa is taken as the research object.The evaluation index system of quinoa planting is constructed according to its planting characteristics,and the weight of each evaluation index is determined,which could lay a foundation for the evaluation of quinoa planting. | Wei LV Jianhan HE Meiyan PEI Zhimin WEI Qiuhong LIU | 2021 | Meteorological and Environmental Research2021，12，2： | 0 |
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