|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer显示文摘MicroRNAs (miRNAs ) 玩在在各种各样的癌症的发展和前进的关键角色。不正常的 miR-9 表示仍然保持模糊,并且卵巢的癌症的变形前进上的一致都没被到达。在这研究,从生物信息学分析的结果证明 E-cadherin mRNA 的 3-UTR 被 miR-9 直接调整。酶记者试金结果证实 miR-9 能直接指向这 3-UTR。在卵巢的癌症织物的 miR-9 和 E-cadherin 表示被 qRT-PCR 确定。移植和侵略被在 SKOV3 和 A2780 愈合弯屈和 Transwell 系统试金检测。qRT-PCR 和西方的污点被执行检测上皮 ? 间充质的联系转变的 mRNA 和蛋白质。Immunofluorescence 技术被用来分析表示和 E-cadherin, N-cadherin,和 vimentin 的 subcellular 本地化。结果证明 miR-9 经常与配对的主要的相比是在变形浆液的卵巢的癌症织物的 upregulated。miR-9 的 Upregulation 能 downregulate E-cadherin 的表示但是 upregulate 间充质的标记(N-cadherin 和 vimentin ) 的表示。miR-9 的 Overexpression 能在卵巢的癌症支持房间移植和侵略,并且这些进程能有效地经由 miR-9 禁止者被禁止。因此,我们的学习证明 miR-9 可以经由指向 E-cadherin 和一条新奇潜在的治疗学的途径控制卵巢的癌症的转移支持卵巢的癌症转移。 | Bo Zhou Hongbin Xu Meng Xia Chaoyang Sun Na Li Ensong Guo Lili Guo Wanying Shan Hao Lu Yifan Wu Yuan Li Degui Yang Danhui Weng Li Meng Junbo Hu Ding Ma Gang Chen Kezhen Li | 2017 | Frontiers of Medicine2017，11，2： | 10 |
| 2 | Genetic characterization and linkage disequilibrium mapping of resistance to gray leaf spot in maize(Zea mays L.)显示文摘Gray leaf spot(GLS),caused by Cercospora zeae-maydis,is an important foliar disease of maize(Zea mays L.)worldwide,resistance to which is controlled by multiple quantitative trait loci(QTL).To gain insights into the genetic architecture underlying the resistance to this disease,an association mapping population consisting of 161 inbred lines was evaluated for resistance to GLS in a plant pathology nursery at Shenyang in 2010 and 2011.Subsequently,a genome-wide association study,using 41,101 single-nucleotide polymorphisms(SNPs),identified 51 SNPs significantly(P<0.001)associated with GLS resistance,which could be converted into 31 QTL.In addition,three candidate genes related to plant defense were identified,including nucleotidebinding-site/leucine-rich repeat,receptor-like kinase genes similar to those involved in basal defense.Two genic SNPs,PZE-103142893 and PZE-109119001,associated with GLS resistance in chromosome bins 3.07 and 9.07,can be used for marker-assisted selection(MAS)of GLS resistance.These results provide an important resource for developing molecular markers closely linked with the target trait,enhancing breeding efficiency. | Liyu Shi Xiangling Lv Jianfeng Weng Hanyong Zhu Changlin Liu Zhuanfang Hao Yu Zhou Degui Zhang Mingshun Li Xiaoke Ci Xinhai Li Shihuang Zhang | 2014 | The Crop Journal2014，2，Z1： | 9 |
| 3 | A sequence of 28S rRNA-derived small RNAs is enriched in mature sperm and various somatic tissues and possibly associates with inflammation显示文摘 | Chu, Chen Yu, Lu Wu, Bin Ma, Li Gou, Lan-Tao He, Miao Guo, Yunli Li, Zhi-Tong Gao, Wei Shi, Huijuan Liu, Mo-Fang Wang, Hongyan Chen, Charlie Degui Drevet, Joel R. Zhou, Yuchuan Zhang, Yonglian | 2017 | Journal of Molecular Cell Biology2017，9，3： | 6 |
| 4 | Prevalence and phylogenetic characterization of canine coronavirus from diseased pet dogs in Beijing,China显示文摘Dear Editor,Canine coronavirus including canine coronavirus(CCoV)and canine respiratory coronavirus(CRCoV)have been recognized as pathogenic viruses to dogs worldwide,causing enteric and respiratory issues(Buonavoglia et al.,2006).Zoonotic transfer of the viruses from the animal kingdom to humans has been repeatedly observed over the past decade, | Shuai Lu Di Zhang Jianfang Zhou Zhaofei Xia Degui Lin Yongliang Lou Wenjie Tan Kun Qin | 2016 | Science China(Life Sciences)2016，59，8： | 5 |
| 5 | PhieCBEs:Plant High-Efficiency Cytidine Base Editors with Expanded Target Range显示文摘Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-mediated genome editing can efficiently produce gene-knockout mutants.On the other hand,CRISPR/Cas-derived base editors offer the ability to induce precise nucleotide substitutions(Komor et al.,2016).Cytidine base editors(CBEs)consist of a cytidine deaminase fused with a Cas9-nickase variant(Cas9n,with a D10A substitu-tion)and can achieve site-specific C-to-T substitution.Similarly,adenine base editors use an adenine deaminase forA-to-G substi-tution.These systems have been used in various organisms(Mishra et al.,2019).However,the Cas9 complex requires target sites containing NGG protospacer adjacent motifs(PAMs),thus restricting selection of potential targets.A number of CBEs have been developed using Cas9 variants(mostly Cas9n),cytidine deaminases(such as rAPOBEC1 and PmCDA1),and uracil glycosylase inhibitor(UGI)domains.These CBEs of the first generation(BE1,rAPOBEC1-dCas9),second generation(BE2,rAPOBEC1-dCas9-UGI),and third generation(BE3,rAPOBEC1-Cas9n-UGI)have moderate editing efficiencies in mammalians(Komor etal.,2016). | Dongchang Zeng Taoli Liu Jiantao Tan Yaling Zhang Zhiye Zheng Bin Wang Degui Zhou Xianrong Xie Menghui Guo Yao-Guang Liu Qinlong Zhu | 2020 | Molecular Plant2020，13，12： | 5 |
| 6 | Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘Histone 离氨酸 methylation 能被 JmjC 包含域的蛋白质在 sequence-state-specific 和 methylation-state-specific 举止移开。然而,底层特性怎么样,决定,酶怎么被调整,大部分是未知的。我们最近发现了那 ceKDM7A,一个哲学博士 -- 并且 JmjC 包含域的蛋白质,是为 H3K9me2 特定的 histone demethylase, H3K27me2,和 PHD 摸绑定到 H3K4me3 指南在 vivo 的 demethylation 活动。为了为酶的活动和 PHD 的功能提供结构的卓见进分子的机制,摸,我们与包含 H3K4me3, H3K9me2,和 H3K27me2 修正的各种各样的联合的单身者或二肽一起在 apo 形式并且在建筑群解决了酶的六水晶结构。结构显示 H3K9me2 和 H3K27me2 以一种类似的方式与 ceKDM7A 交往,并且肽绑定特性被特定的相互作用的一个网络决定。结构的几何测量也揭示了与 PHD 手指和 H3K9me2 界限联系到 JmjC 领域的那 H3K4me3 从二个分开的分子,建议 trans-histone 肽绑定机制。因此,我们的全身的结构的研究重要地由催化领域而且更多揭示底层识别不仅,为 H3K9me2 和 H3K27me2 的 ceDKM7A 的双特性的分子的机制。 | Ying Yang Lulu Hu Ping Wang Haifeng Hou Yan Lin Yi Liu Ze Li Rui Gong Xiang Feng Lu Zhou Wen Zhang Yuhui Dong Huirong Yang Hanqing Lin Yiqin Wang Charlie Degui Chen Yanhui Xu | 2010 | Cell Research2010，20，8： | 3 |
| 7 | Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice显示文摘The mammalian epididymis not only plays a fun dame ntal role in the maturati on of spermatozoa,but also provides protecti on agai nst various stressors.The foremost among these is the threat posed by oxidative stress,which arises from an imbalance in reactive oxygen species and can elicit damage to cellular lipids,proteins,and nucleic acids.In mice,the risk of oxidative damage to spermatozoa is mitigated through the expression and secretion of glutathione peroxidase 5(GPX5)as a major luminal scavenger in the proximal caput epididymidal segment.Accordingly,the loss of GPX5^-/-mediated protection leads to impaired DNA integrity in the spermatozoa of aged Gpx57 mice.To explore the underlying mechanism,we have conducted transcriptomic analysis of caput epididymidal epithelial cells from aged(13 months old)Gpx5^-/-m mice.This analysis revealed the dysregulation of several thousand epididymal mRNA transcripts,in eluding the downregulation of a subgroup of piRNA pathway gen es,in aged Gpx5^-/-mice.In agreeme nt with these fin dings,we also observed the loss of piRNAs,which potentially bind to the P-element-induced wimpy testis(PlWI)-like proteins PIWIL1 and PIWIL2.The absence of these piRNAs was correlated with the elevated mRNA levels of their putative gene targets in the caput epididymidis of Gpx5^-/-mice.Importantly,the oxidative stress response genes tend to have more targeting piRNAs,and many of them were among the top increased genes upon the loss of GPX5^-/-.Taken together,our findings suggest the existence of a previously uncharacterized somatic piRNA pathway in the mammalian epididymis and its possible invoIvement in the aging and oxidative stress-mediated responses. | Chen Chu Lu Yu Joelle Henry-Berger Yan-Fei Ru Ayhan Kocer Alexandre Champroux Zhi-Tong Li Miao He Sheng-Song Xie Wu-Bin Ma Min-Jie Ni Zi-Mei Ni Yun-Li Guo Zhao-Liang Fei Lan-Tao Gou Qiang Liu Samanta Sharma Yu Zhou Mo-Fang Liu Charlie Degui Chen Andrew L Eamens Brett Nixon Yu-Chuan Zhou Joel R Drevet Yong-Lian Zhang | 2020 | Asian Journal of Andrology2020，22，6： | 2 |
| 8 | Comparisons of Metabolic Profiles for Carbohydrates,Amino Acids,Lipids,Fragrance and Flavones During Grain Development in indica Rice Cultivars显示文摘We used a widely targeted metabolomics approach to examine the metabolic changes in three pedigreed indica cultivars(Meixiangzhan 2,Meisizhan and Qixinzhan),with different eating qualities,at 8,15 and 30 d after flowering(DAF)to explore the formation mechanism of rice eating and nutritional qualities at a global metabolic level.A total of 623 metabolites were identified,and results showed that metabolic variations among rice cultivars decreased with grain developmental stage,suggesting that sufficient carbohydrate and amino acid supply during grain development may contribute to excellent rice eating and nutritional quality formation.Lysophosphatidylcholines 19:0 and 16:1 were beneficial for excellent eating quality formation during grain development.Rice fragrance was attributed mainly to spermidine andγ-aminobutyric acid.Rice cultivars with excellent eating quality at 15-30 DAF had relatively higher flavone content,possibly because they had adequate carbohydrate and amino acid contents during grain development.These results provided insight into the mechanisms for establishing rice eating and nutritional qualities during grain formation at a global metabolic level,and can be applied towards improving rice quality. | CHEN Yibo WANG Zhidong WANG Chongrong LI Hong HUANG Daoqiang ZHOU Degui ZHAO Lei PAN Yangyang GONG Rong ZHOU Shaochuan | 2022 | Rice science2022，29，2： | 0 |
| 9 | Design and mechanism of agglomeration of aspirin crystals in pure solvents显示文摘Agglomeration with improved flowability for platy crystals is desirable in pharmaceutical downstream processing. The formation of agglomerates in pure solvents without the aid of bridging liquids is a convenient and low-cost method compared with complex spherical crystallization. In this work, the adhesion free energies between aspirin crystals in six solvents were calculated using Lifshitz-van der Waals acid-base theory to screen suitable solvents for agglomeration. The maximum stirring rate for agglomeration was determined by adhesion forces and dispersion forces. Then the agglomerates of plate-shaped aspirin were successfully prepared in acetone, methanol, ethanol, 2-propanol, and ethylene glycol without additives by simple cooling crystallization. The interactions between solvent and crystal surfaces were also used to explain the outcomes. A feasible mechanism for the agglomeration process of platy crystals was elucidated, involving the adhesion of dominant crystal facets at the beginning. The effect of stirring rate, cooling rate, and initial supersaturation on agglomeration degree and particle size of aspirin agglomerates were studied. The obtained aspirin agglomerates under the optimal conditions exhibited a uniform particle size distribution, a high agglomeration degree, and superior flowability. | Lihong Jia Degui Wu Pingping Cui Ling Zhou Qiuxiang Yin | 2023 | Particuology2023，，11： | 0 |
| 10 | Genomic prediction of yield performance among single-cross maize hybrids using a partial diallel cross design显示文摘Genomic prediction(GP)in plant breeding has the potential to predict and identify the best-performing hybrids based on the genotypes of their parental lines.In a GP experiment,34 elite inbred lines were selected to make 285 single-cross hybrids in a partial-diallel cross design.These lines represented a mini-core collection of Chinese maize germplasm and comprised 18 inbred lines from the Stiff Stalk heterotic group and 16 inbred lines from the Non-Stiff Stalk heterotic group.The parents were genotyped by sequencing and the 285 hybrids were phenotyped for nine yield and yield-related traits at two locations in the summer sowing area(SUS)and three locations in the spring sowing area(SPS)in the main maizeproducing regions of China.Multiple GP models were employed to assess the accuracy of trait prediction in the hybrids.By ten-fold cross-validation,the prediction accuracies of yield performance of the hybrids estimated by the genomic best linear unbiased prediction(GBLUP)model in SUS and SPS were 0.51 and 0.46,respectively.The prediction accuracies of the remaining yield-related traits estimated with GBLUP ranged from 0.49 to 0.86 and from 0.53 to 0.89 in SUS and SPS,respectively.When additive,dominance,epistasis effects,genotype-by-environment interaction,and multi-trait effects were incorporated into the prediction model,the prediction accuracy of hybrid yield performance was improved.The ratio of training to testing population and size of training population optimal for yield prediction were determined.Multiple prediction models can improve prediction accuracy in hybrid breeding. | Ping Luo Houwen Wang Zhiyong Ni Ruisi Yang Fei Wang Hongjun Yong Lin Zhang Zhiqiang Zhou Wei Song Mingshun Li Jie Yang Jianfeng Weng Zhaodong Meng Degui Zhang Jienan Han Yong Chen Runze Zhang Liwei Wang Meng Zhao Wenwei Gao Xiaoyu Chen Wenjie Li Zhuanfang Hao Junjie Fu Xuecai Zhang Xinhai Li | 2023 | The Crop Journal2023，11，6： | 0 |
| 11 | Breeding Effects and Genetic Compositions of a Backbone Parent (Fengbazhan) of Modern indica Rice in China显示文摘Fengbazhan(FBZ)and its derived varieties have been widely cultivated in China,accounting for 22%of China’s paddy fields.Therefore,evaluating the breeding.Effects of FBZ and elucidating its genetic compositions are effective strategies for interpreting the history of modern rice breeding.Using integrative bioinformatics analysis and population validation,we found that the expression of favorable genes on chromosome 6 was important for the breeding of FBZ-derived varieties and these favorable genes were gradually optimized during the breeding process,which was consistent with the conjecture of the‘Rice Core Germplasm Breeding Theory’.For several decades,tremendous efforts have been made by Chinese scientists in rice breeding to improve grain yield,nutritional quality and environmental performance,thereby achieving substantial progress in global food security.Several rice breeding technologies have been developed,including semi-dwarf breeding,utilization of heterosis,launching the‘Super Rice Project’,development of green super rice and molecular design breeding(Qian et al,2016;Bai et al,2018;Tang and Cheng,2018;Yu et al,2022).In southern China,rice can be divided into the early,middle and late rice,and the cultivation area in southern China accounts for approximately 80%of China’s rice cultivation area.Currently,several distinct rice varieties are widely cultivated in southern China. | ZHAO Lei ZHOU Shaochuan WANG Chongrong LI Hong HUANG Daoqiang WANG Zhidong ZHOU Degui CHEN Yibo GONG Rong PAN Yangyang | 2022 | Rice science2022，29，5： | 0 |
| 12 | Stay-Green Panicle Branches Improve Processing Quality of Elite Rice Cultivars显示文摘Rice panicle branches are key routes for transportation of photo synthetic products to grains.Branch senescence is an important trait that the breeders considered when breeding elite rice cultivars in South China.However,little is known about the mechanisms underlying the contributions of stay-green panicle branches to rice yield and quality.Here,we reported stay-green branches that continuously maintained transportation function and provided carbohydrates for basal grain development at the late stages of rice development.This ensured the coordination of development between apical and basal grains,and further improved the processing quality of elite rice.These findings provided strategies for improving the fullness and quality of rice grains and presented an important basis for cultivating elite rice. | CHEN Yibo ZHAO Lei WANG Chongrong LI Hong HUANG Daoqiang WANG Zhidong ZHOU Degui PAN Yangyang GONG Rong ZHOU Shaochuan | 2023 | Rice science2023，30，1： | 0 |
| 13 | OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice显示文摘In plants,lipid transfer proteins(LTPs)transport pollen wall constituents from the tapetum to the exine,a process essential for pollen wall development.However,the functional cooperation of different LTPs in pollen wall development is not well understood.In this study,we have identified and characterized a grassspecific LTP gene,Os LTP47,an important regulator of pollen wall formation in rice(Oryza sativa).Os LTP47 encodes a membrane-localized LTP and in vitro lipid-binding assays confirms that Os LTP47 has lipidbinding activity.Dysfunction of Os LTP47 causes disordered lipid metabolism and defective pollen walls,leading to male sterility.Yeast two-hybrid and pull-down assays reveal that Os LTP47 physically interacts with another LTP,Os C6.These findings suggest that the plasma membrane-localized Os LTP47 may function as a mediator in a lipid transfer relay through association with cytosolic and/or locular Os C6 for pollen wall development and that various LTPs may function in a coordinated manner to transport lipid molecules during pollen wall development. | Libin Chen Chonghui Ji Degui Zhou Xin Gou Jianian Tang Yongjie Jiang Jingluan Han Yao-Guang Liu Letian Chen Yongyao Xie | 2022 | Journal of Genetics and Genomics2022，49，5： | 0 |
| 14 | Bimetallic selenide heterostructure with directional built-in electricfield confined in N-doped carbon nanofibers for superior sodium storage with ultralong lifespan显示文摘Constructing heterostructure is considered as an effective strategy to address the sluggish electronic and ionic kinetics of anode materials for sodium ion batteries(SIBs).However,realizing the orientated growth and uniform distribution of the heterostructure is still a great challenge.Herein,the regulated novel CoSe_(2)/NiSe_(2)heterostructure confined in N-doped carbon nanofibers(CoSe_(2)/NiSe_(2)@N-C)are prepared by using Co/Ni-ZIF template,in which,the CoSe_(2)/NiSe_(2)heterostructures realize uniform distribution on a micro level.Benefiting from the unique heterostructure and N-doped carbon nanofibers,the CoSe_(2)/NiSe_(2)@N-C deliveries superior rate capability and durable cycle lifespan with a reversible capacity of 400.5 mA h g^(-1)after 5000 cycles at 2 A g^(-1).The Na-ion full battery with CoSe_(2)/NiSe_(2)@N-C anode and layered oxide cathode displays a remarkable energy density of 563 W h kg^(-1)with 241.1 W kg^(-1)at 0.1 A g^(-1).The theoretical calculations disclose that the periodic and directional built-in electric-field along with the heterointerfaces of CoSe_(2)/NiSe_(2)@N-C can accelerate electrochemical reaction kinetics.The in(ex)situ experimental measurements reveal the reversible conversion reaction and stable structure of CoSe_(2)/NiSe_(2)@N-C during Na+insertion/extraction.The study highlights the potential ability of precisely controlled heterostructure to stimulate the electrochemical performances of advanced anode for SIBs. | Junying Weng Degui Zou Wenyong Yuan Pengfei Zhou Minghui Ding Jin Zhou Hailin Cong Fangyi Cheng | 2024 | Journal of Energy Chemistry2024，91，4： | 0 |
| 15 | A differentially methylated region of the ZmCCT10 promoter affects flowering time in hybrid maize显示文摘Flowering time(FT) is a key maize domestication trait, variation in which allows maize to grow in a wide range of latitudes. Although previous studies have investigated the genetic control of FT-related traits per se, few studies of FT hybrid performance have been published. We characterized the genomic architecture associated with hybrid performance for FT in a hybrid panel by testcrossing Chang 7–2 with 328Ye478 × Qi319 recombinant inbred lines(RILs). We identified 11 quantitative trait loci(QTL) for hybrid performance in FT-related traits, including a major QTL qFH10 that controls hybrid performance and heterosis in a summer maize-growing region. However, this locus acts in regulating FT traits per se only in a spring maize-growing region. We validated ZmCCT10 as a candidate gene for qFH10 and found that differences between hybrids and their parental lines in DNA methylation in the differentially methylated region(DMR, –700 to –1520) of the ZmCCT10 promoter affected gene expression pattern and thereby FT in the summer maize-growing region. | Zhiqiang Zhou Xin Lu Chaoshu Zhang Mingshun Li Zhuanfang Hao Degui Zhang Hongjun Yong Jienan Han Xinhai Li Jianfeng Weng | 2023 | The Crop Journal2023，11，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员