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| 1 | Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis显示文摘The mechanisms of cotton fiber development and somatic embryogenesis have been explored sys-tematically with microarray and suppression subtractive hybridization. Real-time RT-PCR provides the simultaneous measurement of gene expression in many different samples,with which the data from microarray or others can be confirmed in detail. To achieve accurate and reliable gene expression re-sults,normalization of real-time PCR data against one or several internal control genes is required,which should not fluctuate in different tissues during various stages of development. We assessed the gene expression of 7 frequently used housekeeping genes,including 18S rRNA,Histone3,UBQ7,Actin,Cyclophilin,Gbpolyubiquitin-1 and Gbpolyubiquitin-2,in a diverse set of 21 cotton samples. For fiber developmental series the expression of all housekeeping genes had the same down tendency after 17 DPA. But the expression of the AGP gene(arabinogalactan protein) that has high expression level at the later fiber development stage was up-regulated from 15 to 27 DPA. So the relative absolute quanti-fication should be an efficient and convenient method for the fiber developmental series. The expres-sion of nonfiber tissues series varied not so much against the fiber developmental series. And three best control genes Histone3,UBQ7 and Gbpolyubiquitin-1 have to be used in a combinated way to get better normalization. | TU LiLi ZHANG XianLong LIU DiQiu JIN ShuangXia CAO JingLin ZHU LongFu DENG FengLin TAN JiaFu ZHANG CunBin | 2007 | Chinese Science Bulletin2007，52，22： | 10 |
| 2 | Transgenic Bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower Bt toxin accumulation in seeds显示文摘A promoter of the PNZIP(Pharbitis nil leucine zipper)gene(1.459 kb)was cloned from Pharbitis nil and fused to the GUS(b-glucuronidase)and Bacillus thuringiensis endotoxin(Cry9C)genes.Several transgenic PNZIP::GUS and PNZIP::Cry9C cotton lines were developed by Agrobacterium-mediated transformation.Strong GUS staining was detected in the green tissues of the transgenic PNZIP::GUS cotton plants.In contrast,GUS staining in the reproductive structures such as petals,anther,and immature seeds of PNZIP::GUS cotton was very faint.Two transgenic PNZIP::Cry9C lines and one transgenic cauliflower mosaic virus(Ca MV)35S::Cry9C line were selected for enzyme-linked immunosorbent assay(ELISA)and insect bioassays.Expression of the Cry9C protein in the 35S::Cry9C line maintained a high level in most tissues ranging from24.6 to 45.5μg g^(-1) fresh weight.In green tissues such as the leaves,boll rinds,and bracts of the PNZIP::Cry9C line,the Cry9C protein accumulated up to 50.2,39.7,and 48.3μg g^(-1) fresh weight respectively.In contrast,seeds of the PNZIP::Cry9C line(PZ1.3)accumulated only 0.26μg g^(-1) fresh weight of the Cry9C protein,which was 100 times lower than that recorded for the seeds of the Ca MV 35S::Cry9C line.The insect bioassay showed that the transgenic PNZIP::Cry9C cotton plant exhibited strong resistance to both the cotton bollworm and the pink bollworm.The PNZIP promoter could effectively drive Bt toxin expression in green tissues of cotton and lower accumulated levels of the Bt protein in seeds.These features should allay public concerns about the safety of transgenic foods.We propose the future utility of PNZIP as an economical,environmentally friendly promoter in cotton biotechnology. | Qing Wang Yi Zhu Lin Sun Lebin Li Shuangxia Jin Xianlong Zhang | 2016 | Science China(Life Sciences)2016，59，2： | 5 |
| 3 | Analysis of genes differentially expressed during initial cellular dedifferentiation in cotton显示文摘The early phase of phytohormone induction is a vital stage of somatic embryogenesis. This phase includes a key process for acquiring cellular totipotency through cellular dedifferentiation. To unravel the molecular mechanism of cellular dedifferentiation in cotton, we constructed a cDNA library using the suppression subtractive hybridization method. A total of 286 differential cDNA clones were sequenced and identified. Among these clones, 112 unique ESTs were significantly up-regulated during the early phase of phytohormone induction, and 40.2% of the ESTs were first identified. GST was highly expressed from 6 to 24 h after induction with phytohormone treatment. PRPs were predominantly expressed and exhibited distinct expression patterns in different treatments, suggesting that they are closely related to cellular dedifferentiation in cotton. Putative GhSAMS, GhSAMDC, GhSAHH and GhACO3 involvement in SAM metabolism was identified in this library. The analysis of qRT-PCR showed that two remarkable increased expressions of the four SAM-related genes happened during the early phase of phytohormone induction, and that a highly positive correlation existed between GhSAMS and GhSAHH. The highest expression level of GhSAMS might be associated with its reentry into the cell cycle. The histological observations further showed that some cells accomplished cellular dedifferentiation and division within 72 h in 2,4-D treatment, and that cellular dedifferentiation might be regulated through two alterations in SAM-dependent transmethylation activity in cotton. In addition, the expression patterns of differential genes in different treatments disclosed the complicated interaction between 2, 4-D and kinetin. | ZHU HuaGuo TU LiLi JIN ShuangXia XU Li TAN JiaFu DENG FengLin ZHANG XianLong | 2008 | Chinese Science Bulletin2008，53，23： | 3 |
| 4 | Red fluorescent protein (DsRed2), an ideal reporter for cotton genetic transformation and molecular breeding显示文摘Genes encoding reporter proteins are used as visual marker-assisted tools in genetic transformation as well as plant breeding. In this study, the red fluorescent protein identified in Discosoma sp. coral(DsRed2) was successfully used as a visual marker for cotton genetic engineering. DsRed2 was successfully expressed in two cotton cultivars,JIN668 and YZ1, driven by the Ca MV-35 S promoter via the Agrobacterium-mediated transformation. Our results suggest that DsRed2 expression provides an early-stage selection tool for the transgenic calli via visual observation. Red fluorescence can be detected not only in callus and somatic embryos but also in most tissues and organs of mature plants. The transgenic line Yz-2-DsRed2 was crossed with four different cotton cultivars to assess the transgene heritability and stability in different genetic backgrounds.The heritability of the red color was highly stable when Yz-2-DsRed2 was used as a male parent. The DsRed2 gene expressed 100% in the F_1 hybrids. To investigate the relationship between DsRed2 transcription and DNA methylation, a methylation-specific PCR approach was applied to the Ca MV-35 S promoter region. The results showed a negative association between DNA methylation level in the promoter region and the transgene transcription.Taken together, these findings suggest DsRed2 a visual reporter gene for cotton genetic transformation and molecular breeding programs. | Lin Sun Muna Alariqi Yi Zhu Jianying Li Zelin Li Qing Wang Yajun Li Hangping Rui Xianlong Zhang Shuangxia Jin | 2018 | The Crop Journal2018，6，4： | 2 |
| 5 | A comprehensive overview of cotton genomics,biotechnology and molecular biological studies显示文摘Cotton is an irreplaceable economic crop currently domesticated in the human world for its extremely elongated fiber cells specialized in seed epidermis,which makes it of high research and application value.To date,numerous research on cotton has navigated various aspects,from multi-genome assembly,genome editing,mechanism of fiber development,metabolite biosynthesis,and analysis to genetic breeding.Genomic and 3D genomic studies reveal the origin of cotton species and the spatiotemporal asymmetric chromatin structure in fibers.Mature multiple genome editing systems,such as CRISPR/Cas9,Cas12(Cpf1)and cytidine base editing(CBE),have been widely used in the study of candidate genes affecting fiber development.Based on this,the cotton fiber cell development network has been preliminarily drawn.Among them,the MYB-b HLH-WDR(MBW)transcription factor complex and IAA and BR signaling pathway regulate the initiation;various plant hormones,including ethylene,mediated regulatory network and membrane protein overlap fine-regulate elongation.Multistage transcription factors targeting Ces A 4,7,and 8 specifically dominate the whole process of secondary cell wall thickening.And fluorescently labeled cytoskeletal proteins can observe real-time dynamic changes in fiber development.Furthermore,research on the synthesis of cotton secondary metabolite gossypol,resistance to diseases and insect pests,plant architecture regulation,and seed oil utilization are all conducive to finding more high-quality breeding-related genes and subsequently facilitating the cultivation of better cotton varieties.This review summarizes the paramount research achievements in cotton molecular biology over the last few decades from the above aspects,thereby enabling us to conduct a status review on the current studies of cotton and provide strong theoretical support for the future direction. | Xingpeng Wen Zhiwen Chen Zuoren Yang Maojun Wang Shuangxia Jin Guangda Wang Li Zhang Lingjian Wang Jianying Li Sumbul Saeed Shoupu He Zhi Wang Kun Wang Zhaosheng Kong Fuguang Li Xianlong Zhang Xiaoya Chen Yuxian Zhu | 2023 | Science China(Life Sciences)2023，66，10： | 2 |
| 6 | I- dentification of a novel elite genotype for in vitro cul- ture and genetic transformation of cotton显示文摘 | Jin Shuangxia Zhang Xianlong Nie Yichun | 2006 | Biol Plan- tarum2006，50，4： | 1 |
| 7 | Detection of somaclonal variation of cotton(Gossypium hirsutum) using eytogenetics, Flow Cytometry and Molecular Markers显示文摘 | Shuangxia Jin Ramesh Mushke Huaguo Zhu et d | 2008 | Plant Cell Rep2008，27，： | 1 |
| 8 | Release of Hormones from Conjugates:Chloroplast Expression of -Ghicosidase Results in El- evated Phytohormone Levels Associated with Significant Increase in Biomass and Protection from Aphids or Whiteflies Conferred by Sucrose Esters显示文摘 | Shuangxia Jin Anderson Kanagaraj Dheeraj Verma | 2011 | Plant Physiol2011，155，： | 1 |
| 9 | Somatic embryogenesis and plant regeneration in wild cotton 显示文摘 | Sun Yuqiang Xianlong Jin Shuangxia | 2003 | Plant Cell Tissue and Organ Culture2003，75，： | 1 |
| 10 | Factors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genotypes of Chinese cottons (Gossypium hirsutum L.)显示文摘 | Jiahe Wu Xianlong Zhang Yichun Nie Shuangxia Jin Shaoguang Liang | 2004 | In Vitro Cellular & Developmental Biology - Plant2004，，4： | 1 |
| 11 | Somatic embryogenesis and plant regeneration in wild cotton (Gossypium klotzschianum)显示文摘 | Yuqiang Sun Xianlong Zhang Shuangxia Jin Shaoguang Liang Yichun Nie | 2003 | Plant Cell Tissue and Organ Culture2003，，3： | 1 |
| 12 | An efficient grafting system for transgenic plant recovery in cotton (Gossypium hirsutum L.)显示文摘 | Shuangxia Jin Shaoguang Liang Xianlong Zhang Yichun Nie Xiaoping Guo | 2006 | Plant Cell Tissue and Organ Culture2006，，2： | 1 |
| 13 | Identification and selection of resistance to Bemisia tabaci among 550 cotton genotypes in the field and greenhouse experiments显示文摘Plants have developed sophisticated systems to cope with herbivore challenge, including morphological barriers and secondary metabolites to reduce damage. In this study, 550 Gossypium genotypes were evaluated for whitefly(Bemisia tabaci) resistance in five experiments including two in the field and three in the greenhouse, with23 resistant and 19 susceptible genotypes selected.Whitefly-resistance index determination showed that a leaf having a high density of hairs had resistance to whitfly egg/nymph production. Longer leaf hairs were also important for resistance. This study revealed that okra shaped leaves reduced adult whitefly oviposition preference, while glabrous leaves and high hair density helped not only in the reduction of the adults but also decreased oviposition preference. Gossypol was also observed to be involved in the reduction of adult whitefly development and/or survival. | Lizhen ZHU Jianying LI Zhongping XU Hakim MANGHWAR Sijia LIANG Suli LI Muna ALARIQI Shuangxia JIN Xianlong ZHANG | 2018 | Frontiers of Agricultural Science and Engineering2018，5，2： | 1 |
| 14 | Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion显示文摘 | Yuqiang Sun Xianlong Zhang Yichun Nie Xiaoping Guo Shuangxia Jin Shaoguang Liang | 2004 | Theoretical and Applied Genetics2004，，3： | 1 |
| 15 | Transcriptome and metabolome analysis reveal that oral secretions from Helicoverpa armigera and Spodoptera litura influence wound-induced host response in cotton显示文摘Cotton(Gossypium hirsutum)is an important fiber crop worldwide.Insect attack causes cotton yield and quality losses.However,little is known about the mechanism of cotton response to insect attack.We simulated insect feeding by applying insect oral secretions(OS)to wounds,and combined transcriptome and metabolome analysis to investigate how OS from two major pest species(Helicoverpa armigera and Spodoptera litura)affect cotton defense responses.We found that respectively 12,668 and 13,379 genes were differentially expressed in comparison with wounding alone.On addition of OS,the jasmonic acid signaling pathway was rapidly and strongly induced,whereas genes involved in salicylic acid biosynthesis were downregulated.On constructing a coexpression gene network,we identified a hub gene encoding a leucine-rich repeat receptor kinase that may play an important role in early signal recognition and transduction.OS from the two insect species altered the abundance of flavonoid-related compounds in different patterns.Gossypol remained in lower concentration after OS application than after wounding alone,suggesting a suppressive effect of OS on cotton defense response.This study illustrated transcriptional and metabolic changes of cotton in responding to OS from two chewing insect species,identified potential key response genes,and revealed evidence for OS inhibition of wounding-induced cotton defense response. | Huan Si Hongling Liu Yiwen Sun Zhongping Xu Sijia Liang Bo Li Xiao Ding Jianying Li Qiongqiong Wang Lin Sun Xianlong Zhang Shuangxia Jin | 2020 | The Crop Journal2020，8，6： | 1 |
| 16 | Factors affecting transformation effciency of embryogenic callus of Upland cotton (Gossypium hirsutum) with Agrobacterium tumefaciens 显示文摘 | Shuangxia Jin Xianlong Zhang Shaoguang Liang | 2005 | Plant Cell Tissue and Organ Culture2005，81，： | 1 |
| 17 | Factors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genotypes of Chinese cottons (Gossypium hirsutum L.)显示文摘 | Jiahe Wu Xianlong Zhang Yichun Nie Shuangxia Jin Shaoguang Liang | 2004 | In Vitro Cellular & Developmental Biology - Plant2004，，4： | 1 |
| 18 | Developing an efficient CRISPR-dCas9-TVderived transcriptional activation system to create three novel cotton germplasm materials显示文摘Dear Editor,With the development of CRISPR-Cas technology,an RNAguided CRISPR activation system has been developed in plants,in which a dead Cas9(dCas9)nuclease is fused to transcriptional activators to regulate the transcription of endogenous genes(Li et al.,2017;Liu et al.,2017;Manghwar et al.,2020;Ming et al.,2020;Pan et al.,2021).Precise upregulation of gene transcription is emerging as a promising approach for functional genomics research,molecular breeding,and germplasm innovation.However,there have been no reports on the use of the CRISPR-dCas9 transcriptional activation system in cotton. | Lu Yu Zhanshuai Li Xiao Ding Muna Alariqi Chaojun Zhang Xiangqian Zhu Shuli Fan Longfu Zhu Xianlong Zhang Shuangxia Jin | 2023 | Plant Communications2023，4，4： | 0 |
| 19 | A near-complete genome assembly of Catharanthus roseus and insights into its vinblastine biosynthesis and high susceptibility to the Huanglongbing pathogen显示文摘Dear Editor,Catharanthus roseus,an important medicinal plant native to Madagascar,is the sole source of the two important anticancer drugs vinblastine and vincristine,which are synthesized by the monoterpene-derived indole alkaloid(MIA)pathway(Das et al.,2020).It is also an important model plant for the study of Huanglongbing(HLB),a devastating citrus disease that causes severe economic losses worldwide(Ma et al.,2022).Vincristine is an FDA-approved drug that has been used for acute lymphocytic leukemia,lymphoid blast crisis of chronic myeloid leukemia,and Hodgkin’s/non-Hodgkin’s lymphoma(Below and M Das,2021). | Zhongping Xu Guanying Wang Qiongqiong Wang Xiaoting Li Guangyu Zhang Ali Qurban Can Zhang Yi Zhou Huan Si Lisong Hu Fuqiu Wang Ying Wang Zhitao Tian Wei Chen Shuangxia Jin Fang Ding | 2023 | Plant Communications2023，4，6： | 0 |
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