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| 1 | Construction of a genetic linkage map for cotton based on SRAP显示文摘A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific cross of Handan208 Pima90. A total of 136 primer pairs were used to detect polymorphisms between the two parents and 76 primer pairs with better polymorphisms were picked out to analyze the F2 population. 285 polymorphic bands were generated in total with an average of 3.75 polymorphic bands per pair of primers. The primer pair showing most polymorphic bands was the combination of me3 and em2, which produced 13 polymorphic bands. The 285 loci were used to construct linkage map with MAPMAKER/EXP3.0 and 237 loci were mapped at a LOD≥3.0 on 39 linkage groups. The total length of the map is 3030.7 cM, covering 65.4% of the whole cotton genome, and the average distance between adjacent markers is 12.79 cM. All the markers are distributed evenly among the linkage groups without clustering of loci. This is the first linkage map of cotton comprised of SRAP markers. | LIN Zhongxu, ZHANG Xianlong, NIE Yichun, HE Daohua & WU Maoqing National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China | 2003 | Chinese Science Bulletin2003，48，19： | 111 |
| 2 | High-density Linkage Map of Cultivated Allotetraploid Cotton Based on SSR, TRAP, SRAP and AFLP Markers显示文摘一张高密度的连锁图为从在 Gossypium hirsutum L 之间的 cultivatedallotetraploid 种类的一个种间的十字导出的一张 F_2 人口被构造。并且 G.barbadense L。从种间的十字的 186 个 F_2 个人的一个总数“ CRI36 x 哈尔 7124' 是在包括一个新奇标记系统的 1 252 多态的 loci 的 genotyped,目标区域扩大多型性(陷井) 。地图由 1 097 个标记组成,包括 697 简单顺序重复(SSR ) , 171 个陷井, 129 顺序相关的放大多型性, 98 放大 fragmentlengthpolymorphisms,和二个词法标记,并且跨越了 4 有 4.1cM 的平均遗传距离的 536.7 厘米每标记。用在染色体之中的 45 复制 SSR loci, 13 同源染色体中的 11 个在四倍体棉花被识别。这张地图将为量的特点 loci 并且在棉花的分子的繁殖印射的高分辨率提供一个必要资源。 | Jiwen Yu Shuxun Yu Cairui Lu Wu Wang Shuli Fan Meizhen Song Zhongxu Lin Xianlong Zhang Jinfa Zhang | 2007 | Journal of Integrative Plant Biology2007，49，5： | 29 |
| 3 | FGF21 treatment ameliorates alcoholic fatty liver through activation of AMPK-SIRT1 pathway显示文摘成纤维细胞生长因素 21 (FGF21 ) , FGF 总科的一个最近识别的成员,主要从肝和脂肪质的纸巾被分泌并且在改进新陈代谢的症候群和动态平衡起一个重要作用。这研究的目的是在含酒精的脂肪肝疾病(AFLD ) 评估 FGF21 的角色并且决定它是否在 AFLD 上有治疗学的效果。在这份报纸,我们在长期的乙醇 gavage 和对待白酒的 HepG2 房间的一个鼠科的模型在导致白酒的肝损害上测试了 FGF21 的效果。男千米;公里老鼠每天收到了 5 g/kg 乙醇 gavage 的单个剂量 6 个星期,它导致了重要脂肪肝和肝损害。导致白酒的脂肪肝房间模型被在 75 公里的最后的集中增加乙醇进 HepG2 房间文化的媒介 9 天完成。结果与 recombinant FGF21 显示出那个处理在长期的乙醇 gavage 和对待白酒的 HepG2 房间的一个鼠科的模型的改善含酒精的脂肪肝和肝损害。另外, FGF21 处理下面调整丰满的酸的肝的表示合成的关键酶,激活的肝的 AMPK-SIRT1 小径和显著地下面调整的肝的氧化压力蛋白质。一起拿, FGF21 由 AMPK-SIRT1 小径的激活在 vitro 并且在 vivo 改正 AFLD 的多重新陈代谢的参数。 | Shenglong Zhu Lei Ma Yunzhou Wu Xianlong Ye Tianyuan Zhang Qingyang Zhang Lubna Muhi Rasoul Yunye Liu MO Guo Bing Zhou Guiping Ren Deshan Li | 2014 | Acta Biochimica et Biophysica Sinica2014，46，12： | 11 |
| 4 | Association between coexisting intracranial artery and extracranial carotid artery atherosclerotic diseases and ipsilateral cerebral infarction: a Chinese Atherosclerosis Risk Evaluation (CARE- II) study显示文摘Background To evaluate the association between coexisting intracranial and extracranial carotid artery atherosclerotic diseases and ipsilateral acute cerebral infarct(ACI)in symptomatic patients by using magnetic resonance(MR)vessel wall imaging.Methods Symptomatic patients were recruited from a cross-sectional,multicentre study of Chinese Atherosclerosis Risk Evaluation(CARE-II).All patients underwent MR imaging for extracranial carotid arterial wall,intracranial artery and brain.Coexisting intracranial stenosis≥50%and extracranial carotid artery mean wall thickness(MWT)≥1 mm and plaque compositions at the same side were evaluated and the ipsilateral ACI was identified.The association between coexisting atherosclerotic diseases and ACI was evaluated using logistic regression.Results 351 patients were recruited.Patients with ipsilateral ACI had significantly greater prevalence of coexisting intracranial stenosis≥50%and carotid MWT≥1 mm(20.5%vs 4.9%,p<0.001),calcification(15.1%vs 4.4%,p=0.001)and lipid-rich necrotic core(LRNC)(19.2%vs 7.8%,p=0.002)compared with those without.Coexisting intracranial artery stenosis≥50%and carotid MWT≥1 mm(OR 5.043,95%CI 2.378 to 10.694;p<0.001),calcification(OR 3.864,95%CI 1.723 to 8.664;p=0.001)and LRNC(OR 2.803,95%CI 1.455 to 5.401;p=0.002)were significantly associated with ipsilateral ACI.After adjusting for confounding factors,the aforementioned associations remained statistically significant(intracranial stenosis≥50%coexisting with carotid MWT≥1 mm:OR 4.313,95%CI 1.937 to 9.601,p<0.001;calcification:OR 3.606,95%CI 1.513 to 8.593,p=0.004;LRNC:OR 2.358,95%CI 1.166 to 4.769,p=0.017).Conclusions Coexistence of intracranial artery severe stenosis and extracranial carotid artery large burden and intraplaque components of calcification and LRNC are independently associated with ipsilateral ACI.Trial registration number http://gffzz1caf53c915c04334su55uk6cqubwc6f9u.ffgz.tsg.suse.edu.cn/.Unique identifier:NCT02017756. | Chunxiu Jiang Jing Zhang Jianbin Zhu Xianlong Wang Zhibo Wen Xihai Zhao Chun Yuan On behalf of CARE-II Investigators | 2021 | Stroke & Vascular Neurology2021，6，4： | 11 |
| 5 | Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis显示文摘The mechanisms of cotton fiber development and somatic embryogenesis have been explored sys-tematically with microarray and suppression subtractive hybridization. Real-time RT-PCR provides the simultaneous measurement of gene expression in many different samples,with which the data from microarray or others can be confirmed in detail. To achieve accurate and reliable gene expression re-sults,normalization of real-time PCR data against one or several internal control genes is required,which should not fluctuate in different tissues during various stages of development. We assessed the gene expression of 7 frequently used housekeeping genes,including 18S rRNA,Histone3,UBQ7,Actin,Cyclophilin,Gbpolyubiquitin-1 and Gbpolyubiquitin-2,in a diverse set of 21 cotton samples. For fiber developmental series the expression of all housekeeping genes had the same down tendency after 17 DPA. But the expression of the AGP gene(arabinogalactan protein) that has high expression level at the later fiber development stage was up-regulated from 15 to 27 DPA. So the relative absolute quanti-fication should be an efficient and convenient method for the fiber developmental series. The expres-sion of nonfiber tissues series varied not so much against the fiber developmental series. And three best control genes Histone3,UBQ7 and Gbpolyubiquitin-1 have to be used in a combinated way to get better normalization. | TU LiLi ZHANG XianLong LIU DiQiu JIN ShuangXia CAO JingLin ZHU LongFu DENG FengLin TAN JiaFu ZHANG CunBin | 2007 | Chinese Science Bulletin2007，52，22： | 10 |
| 6 | Activated carbon coated palygorskite as adsorbent by activation and its adsorption for methylene blue显示文摘An activation process for developing the surface and porous structure of palygorskite/carbon(PG/C) nanocomposite using ZnC l2 as activating agent was investigated. The obtained activated PG/C was characterized by X-ray diffraction(XRD), Fourier transform infrared spectroscopy(FTIR), field-emission scanning electron microscopy(SEM), and Brunauer–Emmett–Teller analysis(BET) techniques. The effects of activation conditions were examined,including activation temperature and impregnation ratio. With increased temperature and impregnation ratio, the collapse of the palygorskite crystal structure was found to accelerate and the carbon coated on the surface underwent further carbonization. XRD and SEM data confirmed that the palygorskite structure was destroyed and the carbon structure was developed during activation. The presence of the characteristic absorption peaks of C_C and C–H vibrations in the FTIR spectra suggested the occurrence of aromatization. The BET surface area improved by more than 11-fold(1201 m2/g for activated PG/C vs. 106 m2/g for PG/C) after activation, and the material appeared to be mainly microporous. The maximum adsorption capacity of methylene blue onto the activated PG/C reached 351 mg/g. The activated PG/C demonstrated better compressive strength than activated carbon without palygorskite clay. | Xianlong Zhang Liping Cheng Xueping Wu Yingzhao Tang Yucheng Wu | 2015 | Journal of Environmental Sciences2015，27，7： | 9 |
| 7 | Design and Fabrication of a Multi-electrode Metal-core Piezoelectric Fiber and Its Application as an Airflow Sensor显示文摘 | Yixiang Bian Yanjun Zhang Xianlong Xia | 2016 | Journal of Bionic Engineering2016，13，3： | 9 |
| 8 | Studies of new EST-SSRs derived from Gossypium barbadense显示文摘Existing cotton EST-SSR markers are mostly derived from Gossypium arboreum and Gossypium hir-sutum, but EST-SSR markers from Gossypium barbadense are scarce. One hundred and nineteen EST-SSRs were developed based on 98 unique ESTs from a cDNA library constructed in our laboratory using developing fibers from G. barbadense cv. Pima3-79. Among the SSRs, trinucleotide AAG appeared at a high frequency of 11.76%. 36 accessions (consisting of 13 diploids of the A genome, 11 diploids of the D genome and 12 allotetraploids of the AD genome) were employed to test new EST-SSRs. 76 EST-SSRs were successfully amplified, and 313 polymorphic fragments were yielded, with an average of 4.11 fragments per primer pair. The PIC ranged from 0.17 to 0.95 with an average of 0.53. Based on Jaccard’s genetic similarity coefficient, these 36 accessions were clustered into three groups. 21 EST-SSRs exhibited polymorphisms in BC1 population ((Emian22 × Pima3-79) × Emian22), 24 polymor- phic loci were generated, while 22 of the 24 polymorphic loci were integrated with our interspecific BC1 backbone genetic linkage map, and anchored in 12 chromosomes. This study effectively proved that EST-SSRs from G. barbadense are valuable for genetic diversity analysis and genetic mapping. | ZHANG YanXin LIN ZhongXu LI Wu TU LiLi NIE YiChun ZHANG XianLong | 2007 | Chinese Science Bulletin2007，52，18： | 7 |
| 9 | Improvement of the activity and SO_(2) tolerance of Sb-modified Mn/PG catalysts for NH_(3)-SCR at a low temperature显示文摘A series of MnM/palygorskite(PG)(M=La,W,Mo,Sb,Mg)catalysts was prepared by the wetness co-impregnation method for low-temperature selective catalytic reduction(SCR)of NO with NH_3.Conversion efficiency followed the order Sb>Mo>La>W>Mg.A combination of various physico-chemical techniques was used to investigate the influence of Sb-modified Mn/PG catalysts.MnSb_(0.156)/PG catalyst showed highest NO conversion at low temperatures in the presence of SO_(2) which reveals that addition of Sb oxides effectively enhances the SCR activity of catalysts.A SO_(2) step-wise study showed that MnSb_(0.156)/PG catalyst displays higher durable resistance to SO_(2) than Mn/PG catalyst,where the sulfating of active phase is greatly inhibited after Sb doping.Scanning electron microscopy and X-ray diffraction results showed that Sb loading enhances the dispersion of Mn oxides on the carrier surface.According to the results of characterization analyses,it is suggested that the main reason for the deactivation of Mn/PG is the formation of manganese sulfates which cause the permanent deactivation of Mn-based catalysts.For Sb-doped Mn/PG catalyst,SO_(x) ad-species formed were mainly combined with SbO_(x) rather than MnO_(x).This preferential interaction between SbO_(x) and SO_(2) effectively shields the MnO_(x) as active species from being sulfated by SO_(2) resulting in the improvement of SO_(2) tolerance on Sb-added catalyst.Multiple information support that,owing to the addition of Sb,original formed MnO_(x) crystallite has been completely transformed into highly dispersed amorphous phase accounting for higher SCR activity. | Xianlong Zhang Shuangshuang Lv Xincheng Zhang Kesong Xiao Xueping Wu | 2021 | Journal of Environmental Sciences2021，33，3： | 6 |
| 10 | Suppression of the homeobox gene HDTF_1 enhances resistance to Verticillium dahliae and Botrytis cinerea in cotton显示文摘Development of pathogen-resistant crops, such as fungus-resistant cotton, has significantly reduced chemical application and improved crop yield and quality. However, the mechanism of resistance to cotton pathogens such as Verticillium dahliae is still poorly understood. In this study,we characterized a cotton gene(HDTF1) that was isolated following transcriptome profiling during the resistance response of cotton to V. dahliae. HDTF1 putatively encodes a homeodomain transcription factor, and its expression was found to be down-regulated in cotton upon inoculation with V. dahliae and Botrytis cinerea. To characterise the involvement of HDTF1 in the response to these pathogens, we used virusinduced gene silencing(VIGS) to generate HDTF1-silenced cotton. VIGS reduction in HDTF1 expression significantly enhanced cotton plant resistance to both pathogens. HDTF1 silencing resulted in activation of jasmonic acid(JA)-mediated signaling and JA accumulation. However, the silenced plants were not altered in the accumulation of salicylic acid(SA) or the expression of marker genes associated with SA signaling. These results suggest that HDTF1 is a negative regulator of the JA pathway, and resistance to V. dahliae and B. cinerea can be engineered by activation of JA signaling. | Wei Gao Lu Long Li Xu Keith Lindsey Xianlong Zhang Longfu Zhu | 2016 | Journal of Integrative Plant Biology2016，58，5： | 5 |
| 11 | SNORD126 promotes HCC and CRC cell growth by activating the PI3K-AKT pathway through FGFR2显示文摘小核仁的 RNA (snoRNA ) 机能障碍与癌症开发被联系了。SNORD126 是在它的主机基因的 introns 5-6 以内被编码的孤儿 C/D 框 snoRNA, cyclin 交往 B1 蛋白质 1 (CCNB1IP1 ) 。SNORD126 触发的联系癌症的分子的机制充分没被理解。这里,我们证明 SNORD126 高度在 hepatocellular 癌(HCC ) 被表示, colorectal 癌症(CRC ) 病人取样。SNORD126 在裸体老鼠增加了 Huh-7 和 SW480 细胞生长和 tumorigenicity。SNORD126 击倒禁止的 HepG2 和 LS174T 房间生长。我们证实 SNORD126 没与 miRNA 活动被处理成小 RNA。而且, SNORD126 没在 HCC 样品与 CCNB1IP1 显示出重要表示关联或调整 CCNB1IP1 表示。我们的基因表示侧面分析显示了基因经常印射到 PI3K-AKT 小径的那 SNORD126-upregulated。SNORD126 overexpression 增加了 phosphorylated AKT, GSK-3,和 p70S6K 的层次并且提高了成纤维细胞生长因素受体 2 (FGFR2 ) 表示。在 SNORD126-overexpressing Huh-7 细胞的 FGFR2 的调停 siRNA 的击倒或调停 AZD4547 的 inactivation 禁止了 AKT phosphorylation 并且压制了细胞生长。这些调查结果在癌症为 SNORD126 显示一个 oncogenic 角色并且作为一个治疗学的目标建议它的潜力。 | Xianlong Fang Dongmei Yang Hongping Luo Shuai Wu Wenjie Dong Jing Xiao Sujing Yuan Aimin Ni Kang-Jian Zhang Xin-Yuan Liu Liang Chu | 2017 | Journal of Molecular Cell Biology2017，9，3： | 5 |
| 12 | A 2-bp insertion(c.6768insCC) in MC1R causes recessive white coat color in Bama miniature pigs显示文摘Coat color is an important characteristic of various breeds of domestic animal species.Variation in farm animal coat color is of considerable interest for concealment,communication and protection against solar radiation(Slominski et al.,2004).It also plays an important role in the regulation of physiological processes(Miyagi and Terai,2013).Bama miniature pigs are a native breed | Qitao Jia Chunwei Cao Hai Tang Ying Zhang Qiantao Zheng Xiao Wang Rui Zhang Xianlong Wang Ailing Luo Hong Wei Anming Meng Qi Zhou Hongmei Wang Jianguo Zhao | 2017 | Journal of Genetics and Genomics2017，44，4： | 5 |
| 13 | Transgenic Bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower Bt toxin accumulation in seeds显示文摘A promoter of the PNZIP(Pharbitis nil leucine zipper)gene(1.459 kb)was cloned from Pharbitis nil and fused to the GUS(b-glucuronidase)and Bacillus thuringiensis endotoxin(Cry9C)genes.Several transgenic PNZIP::GUS and PNZIP::Cry9C cotton lines were developed by Agrobacterium-mediated transformation.Strong GUS staining was detected in the green tissues of the transgenic PNZIP::GUS cotton plants.In contrast,GUS staining in the reproductive structures such as petals,anther,and immature seeds of PNZIP::GUS cotton was very faint.Two transgenic PNZIP::Cry9C lines and one transgenic cauliflower mosaic virus(Ca MV)35S::Cry9C line were selected for enzyme-linked immunosorbent assay(ELISA)and insect bioassays.Expression of the Cry9C protein in the 35S::Cry9C line maintained a high level in most tissues ranging from24.6 to 45.5μg g^(-1) fresh weight.In green tissues such as the leaves,boll rinds,and bracts of the PNZIP::Cry9C line,the Cry9C protein accumulated up to 50.2,39.7,and 48.3μg g^(-1) fresh weight respectively.In contrast,seeds of the PNZIP::Cry9C line(PZ1.3)accumulated only 0.26μg g^(-1) fresh weight of the Cry9C protein,which was 100 times lower than that recorded for the seeds of the Ca MV 35S::Cry9C line.The insect bioassay showed that the transgenic PNZIP::Cry9C cotton plant exhibited strong resistance to both the cotton bollworm and the pink bollworm.The PNZIP promoter could effectively drive Bt toxin expression in green tissues of cotton and lower accumulated levels of the Bt protein in seeds.These features should allay public concerns about the safety of transgenic foods.We propose the future utility of PNZIP as an economical,environmentally friendly promoter in cotton biotechnology. | Qing Wang Yi Zhu Lin Sun Lebin Li Shuangxia Jin Xianlong Zhang | 2016 | Science China(Life Sciences)2016，59，2： | 5 |
| 14 | Pathogenic analysis in different types of orthopedic implant infections显示文摘 | Shen Hao Tang Jin Mao Yanjie Wang Qiaojie Wang Jianqiang Zhang Xianlong Jiang Yao | 2014 | Chinese Medical Journal2014，，15： | 4 |
| 15 | Clonal analysis reveals remarkable functional heterogeneity during hematopoietic stem cell emergence显示文摘 | Hui Ye Xiaobo Wang Zongcheng Li Fan Zhou Xianlong Li Yanli Ni Weijing Zhang Fuchou Tang Bing Liu Yu Lan | 2017 | Cell Research2017，27，8： | 4 |
| 16 | Hepatic encephalopathy complicated with hyponatremia and acid-base disturbance and its prognosis显示文摘Objective:To improve the diagnosis and therapeutic effect of occurrence and development of hyponatremia and disorder of acid-base balance among patients with hepatic encephalopathy(HE) by elucidating the regularity and mechanism,as well as its influence on prognosis.Methods:327 HE patients admitted to our hospital from January 1990 to June 2010 were enrolled.Meanwhile 316 patients hospitalized in the medical department of the same hospital were chosen as the control group.Patients in both groups were given the same methods to measure arterial blood gas parameters(pH value,PaCO2,[HCO3-],TCO2,BE and SaO2),blood biochemistry([Na+],[K+],[Cl-]),liver function,kidney function and blood glucose,serum sodium,and thereupon tocalculate the anion gap(AG) and the potential [HCO3-],and acid-base balance disorder.Results:Among the 327 HE patients,hyponatremia was found in 188 cases(57.4%),of whom 132 patients died(70.2%).While among the 316 patients in control group,68 presented with hyponatremia(21.5%),and 19 died(27.9%).The incidence and mortality were significantly different between the two groups(P<0.001).All the 327 patients presented with different degrees of acid-base balance disorder and 178 died(54.4%),in whom 164(50.2%) belonged to simple acid-base balance disorder and 74(45.1%) died,136(41.6%) were dual acid-base balance disorder and 80(58.8%) died,27(8.2%) were triple acid-base disturbance and 24(88.9%) died.Whereas in the control group only 83 patients(26.2%) were recognized as simple and dual acid-base balance disorder,and 18(21.7%) died.There was higher incidence of acid-base balance disorder and mortality rate in HE group than control one(P<0.001).Conclusion:Hyponatremia is valuable to judge HE patients' prognosis.The key parameters in the judgment and evaluation on acid-base balance disorder among HE patients are the change of pH values and serum electrolyte values.When pH value ≤ 7.30 or > 7.55,it generally suggests a poor prognosis. | Ren Chengshan Wang Lei Zhao Xiaoyan Yang Shiming Zhang Yanqi Wang Xiwen Bo Jianying Zhang Pengbin Guo Hong Ling Xianlong Li Yihui Da Siping Xie Xia Ren Wei Li Chunhua Qian Guisheng | 2012 | Journal of Medical Colleges of PLA(China)2012，27，3： | 3 |
| 17 | Analysis of genes differentially expressed during initial cellular dedifferentiation in cotton显示文摘The early phase of phytohormone induction is a vital stage of somatic embryogenesis. This phase includes a key process for acquiring cellular totipotency through cellular dedifferentiation. To unravel the molecular mechanism of cellular dedifferentiation in cotton, we constructed a cDNA library using the suppression subtractive hybridization method. A total of 286 differential cDNA clones were sequenced and identified. Among these clones, 112 unique ESTs were significantly up-regulated during the early phase of phytohormone induction, and 40.2% of the ESTs were first identified. GST was highly expressed from 6 to 24 h after induction with phytohormone treatment. PRPs were predominantly expressed and exhibited distinct expression patterns in different treatments, suggesting that they are closely related to cellular dedifferentiation in cotton. Putative GhSAMS, GhSAMDC, GhSAHH and GhACO3 involvement in SAM metabolism was identified in this library. The analysis of qRT-PCR showed that two remarkable increased expressions of the four SAM-related genes happened during the early phase of phytohormone induction, and that a highly positive correlation existed between GhSAMS and GhSAHH. The highest expression level of GhSAMS might be associated with its reentry into the cell cycle. The histological observations further showed that some cells accomplished cellular dedifferentiation and division within 72 h in 2,4-D treatment, and that cellular dedifferentiation might be regulated through two alterations in SAM-dependent transmethylation activity in cotton. In addition, the expression patterns of differential genes in different treatments disclosed the complicated interaction between 2, 4-D and kinetin. | ZHU HuaGuo TU LiLi JIN ShuangXia XU Li TAN JiaFu DENG FengLin ZHANG XianLong | 2008 | Chinese Science Bulletin2008，53，23： | 3 |
| 18 | Regeneration and identification of interspecific asymmetric somatic hybrids obtained by donor-recipient fusion in cotton显示文摘Asymmetric hybrids between Gossypium hirsutum (YZ-1) and G. davidsonii were obtained by donor-recipient fusion. YZ-1 was considered the recipient and was pretreated with iodoacetamide (IOA), while G. davidsonii was considered the donor and was irradiated with ultraviolet (UV) before fusion. YZ-1 protoplasts stopped growth when treated with 0.5 mmol/L IOA for 20 min, and G. davidsonii protoplasts stopped growth when irradiated with 38.7 J/cm2 UV for 30 s. Asymmetric somatic hybrids were obtained by electrofusion between the separately treated protoplasts of the 2 species. The regenerated plants were identified by morphological, cytological, and molecular analysis. Most regenerated plants derived from fused protoplasts displayed new morphology; some were intermediate between the two parents and a few displayed recipient-like morphology. Chromosome numbers in these somatic hybrids mostly ranged from 40 to 73. The hybridity was confirmed by random amplified polymorphic DNA and simple sequence repeat analysis. Organelle DNA inheritance of the YZ-1 and G. davidsonii somatic hybrid was investigated by cleaved amplified polymorphism sequence and chloroplast simple sequence repeat analysis, which indicated that recombination and rearrangements might have occurred in some regions of mitochondrial and chloroplastic DNA. This is the first report of completely asymmetric hybrid production via donor–recipient fusion between G. hirsutum and G. davidsonii, which is a novel case in hybrid production following the symmetric fusion and asymmetric fusion based on UV irradiation in cotton. | FU LiLi YANG XiYan ZHANG XianLong WANG ZhiWei FENG ChangHui LIU ChuanXiang JIANG Pei-Yong ZHANG JinLong | 2009 | Chinese Science Bulletin2009，54，17： | 3 |
| 19 | Evaluation of channel-like porous-structured titanium in mechanical properties and osseointegration显示文摘The porous titanium with a channel-like pore structure fabricated by infiltration casting followed by selectively dissolving the precursor woven three dimensional(3D)structure technique was comprehensively investigated by means of mechanical tests,in vitro and in vivo evaluation.Such porous structure exhibited superiority in compressive,tensile strength and osseointegration.At 40%porosity,the average compressive and tensile strength reached about 145MPa and 85 MPa,which was superior to that of other porous titanium,e.g.,Selective Laser Melting or powder sintered ones,and was comparable to that of the human cortical bone.Without any bioactive surface treatment,this porous titanium exhibited good cell adhesion,rapid cell proliferation and excellent osseointegration.Based on the study,the 0.4mm pore size resulted in the most rapid cell proliferation and the maximal BV/TV ratio and trabecular bone number of the new bone that ingrew into the porous titanium.To balance the excellent osseointegration and adequate mechanical properties,the optimal structural parameters were 0.4mmpore size with 40%porosity.This porous titanium is very promising for orthopedic applications where compressive and tensile load-bearing is extremely important. | Dong Wang Guo He Ye Tian Ning Ren Jiahua Ni Wei Liu Xianlong Zhang | 2020 | Journal of Materials Science & Technology2020，44，9： | 2 |
| 20 | PM_(2.5)and water-soluble inorganic ion concentrations decreased faster in urban than rural areas in China显示文摘We investigated variations of PM_(2.5)and water-soluble inorganic ions chemical characteristics at nine urban and rural sites in China using ground-based observations.From 2015 to 2019,mean PM_(2.5)concentration across all sites decreased by 41.9μg/m~3with a decline of 46%at urban sites and 28%at rural sites,where secondary inorganic aerosol(SIAs)contributed to 21%(urban sites)and 17%(rural sites)of the decreased PM_(2.5).SIAs concentrations underwent a decline at urban locations,while sulfate(SO_(4)^(2–)),nitrate(NO_(3)^(–)),and ammonium(NH_(4)^(+))decreased by 49.5%,31.3%and 31.6%,respectively.However,only SO_(4)^(2–)decreased at rural sites,NO_(3)^(–)increased by 21%and NH_(4)^(+)decreased slightly.Those changes contributed to an overall SIAs increase in 2019.Higher molar ratios of NO_(3)^(–)to SO_(4)^(2–)and NH_(4)^(+)to SO_(4)^(2–)were observed at urban sites than rural sites,being highest in the heavily polluted days.Mean molar ratios of NH_(3)/NH_xwere higher in 2019 than 2015 at both urban and rural sites,implying increasing NH_xremained as free NH_(3).Our observations indicated a slower transition from sulfate-driven to nitrate-driven aerosol pollution and less efficient control of NO_(x)than SO_(2)related aerosol formation in rural regions than urban regions.Moreover,the common factor at urban and rural sites appears to be a combination of lower SO_(4)^(2–)levels and an increasing fraction of NO_(3)^(–)to PM_(2.5)under NH_(4)^(+)-rich conditions.Our findings imply that synchronous reduction in NO_(x)and NH_(3)emissions especially rural areas would be effective to mitigate NO_(3)^(–)-driven aerosol pollution. | Yangyang Zhang Aohan Tang Chen Wang Xin Ma Yunzhe Li Wen Xu Xiaoping Xia Aihua Zheng Wenqing Li Zengguo Fang Xiufen Zhao Xianlong Peng Yuping Zhang Jian Han Lijuan Zhang Jeffrey L.Collett Jr Xuejun Liu | 2022 | Journal of Environmental Sciences2022，34，12： | 2 |
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