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| 1 | Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis显示文摘The mechanisms of cotton fiber development and somatic embryogenesis have been explored sys-tematically with microarray and suppression subtractive hybridization. Real-time RT-PCR provides the simultaneous measurement of gene expression in many different samples,with which the data from microarray or others can be confirmed in detail. To achieve accurate and reliable gene expression re-sults,normalization of real-time PCR data against one or several internal control genes is required,which should not fluctuate in different tissues during various stages of development. We assessed the gene expression of 7 frequently used housekeeping genes,including 18S rRNA,Histone3,UBQ7,Actin,Cyclophilin,Gbpolyubiquitin-1 and Gbpolyubiquitin-2,in a diverse set of 21 cotton samples. For fiber developmental series the expression of all housekeeping genes had the same down tendency after 17 DPA. But the expression of the AGP gene(arabinogalactan protein) that has high expression level at the later fiber development stage was up-regulated from 15 to 27 DPA. So the relative absolute quanti-fication should be an efficient and convenient method for the fiber developmental series. The expres-sion of nonfiber tissues series varied not so much against the fiber developmental series. And three best control genes Histone3,UBQ7 and Gbpolyubiquitin-1 have to be used in a combinated way to get better normalization. | TU LiLi ZHANG XianLong LIU DiQiu JIN ShuangXia CAO JingLin ZHU LongFu DENG FengLin TAN JiaFu ZHANG CunBin | 2007 | Chinese Science Bulletin2007，52，22： | 10 |
| 2 | Gene characterization and molecular pathway analysis of reverse thermosensitive genic male sterility in eggplant(Solanum melongena L.)显示文摘The naturally occurring mutant eggplant line 05ms was identified with reverse thermosensitive genic male sterility(rTGMS),but its temperature-responsive fertility mechanisms remain largely unknown.Here,we studied the flower morphology,anther cellular structure,and genome-wide gene expression of this rTGMS line.Candidate genes for thermosensitive male sterility during the microspore development of 05ms and the temperature-insensitive line S63 under low-temperature(LT)and high-temperature(HT)conditions were identified.Under LT,tapetum cells were vacuolated and had delayed disintegration in 05ms.RNA-seq analysis indicated that DEGs were enriched in the KEGG pathways‘plant hormone signal transduction’,‘starch and sucrose metabolism’,and‘phenylpropanoid biosynthesis’.We identified two genes,4CLL1(Sme2.5_00368.1_g00010.1)and CKI1(Sme2.5_10056.1_g00002.1),which could potentially regulate eggplant anther development and may be candidate genes for rTGMS.Finally,we propose a working model of anther abortion for rTGMS in eggplant.CKI1 responds to LT stress and causes expression changes in genes related to anther development,such as 4CLL1,and the cellular structure of the tapetum becomes abnormal,causing male sterility.The findings of this study explain the underlying molecular mechanisms of male sterility in eggplant rTGMS lines. | Bing Li Xueping Chen Yanrong Wu Aixia Gu Jingjing Zhang Shuangxia Luo Xiurui Gao Jianjun Zhao Xiuqing Pan Shuxing Shen | 2019 | Horticulture Research2019，6，1： | 5 |
| 3 | Transgenic Bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower Bt toxin accumulation in seeds显示文摘A promoter of the PNZIP(Pharbitis nil leucine zipper)gene(1.459 kb)was cloned from Pharbitis nil and fused to the GUS(b-glucuronidase)and Bacillus thuringiensis endotoxin(Cry9C)genes.Several transgenic PNZIP::GUS and PNZIP::Cry9C cotton lines were developed by Agrobacterium-mediated transformation.Strong GUS staining was detected in the green tissues of the transgenic PNZIP::GUS cotton plants.In contrast,GUS staining in the reproductive structures such as petals,anther,and immature seeds of PNZIP::GUS cotton was very faint.Two transgenic PNZIP::Cry9C lines and one transgenic cauliflower mosaic virus(Ca MV)35S::Cry9C line were selected for enzyme-linked immunosorbent assay(ELISA)and insect bioassays.Expression of the Cry9C protein in the 35S::Cry9C line maintained a high level in most tissues ranging from24.6 to 45.5μg g^(-1) fresh weight.In green tissues such as the leaves,boll rinds,and bracts of the PNZIP::Cry9C line,the Cry9C protein accumulated up to 50.2,39.7,and 48.3μg g^(-1) fresh weight respectively.In contrast,seeds of the PNZIP::Cry9C line(PZ1.3)accumulated only 0.26μg g^(-1) fresh weight of the Cry9C protein,which was 100 times lower than that recorded for the seeds of the Ca MV 35S::Cry9C line.The insect bioassay showed that the transgenic PNZIP::Cry9C cotton plant exhibited strong resistance to both the cotton bollworm and the pink bollworm.The PNZIP promoter could effectively drive Bt toxin expression in green tissues of cotton and lower accumulated levels of the Bt protein in seeds.These features should allay public concerns about the safety of transgenic foods.We propose the future utility of PNZIP as an economical,environmentally friendly promoter in cotton biotechnology. | Qing Wang Yi Zhu Lin Sun Lebin Li Shuangxia Jin Xianlong Zhang | 2016 | Science China(Life Sciences)2016，59，2： | 5 |
| 4 | EMBRITTLEMENT OF Fe-Cr-Al ALLOYS AND EFFECT OF Y ADDITION显示文摘A high temperature embrittlement occures in Fe-15Cr-4AI and Fe-20Cr-4Al alloys treatedover 1000 C.It is caused mainly by 475℃ brittleness during cooling.When a secondarytreatment at 500℃ was conducted,the 475℃ brittleness developed very rapidly.After only15 rain of the treating,little plasticity was remained for all samples pretreuted at the temper-atures over 1000℃ and cooled in water.However,no 475℃ brittleness was generated whenpretreating temperature was below 1000℃,Addition of Yttrium can inhibit not only graingrowth,but also 475℃ brittleness.For the alloys with 0.2—0.4 wt-%Y,after treating at800—1300℃,the plasticity did not decrease and the properties of furnace cooled sampleswere not lower than those of water cooled samples. | LI Bei WU Shuangxia LI Dianchen Baotou Research Institute of Rare Earth Ministry of Metallurgical Industry,Baotou,ChinaMA Ruzhang University Science and Technology Beijing,Beijing,China Baotou Research Institute of Rare Earth,Ministry of Metallurgical Industry,Baotou 014010,China | 1993 | Acta Metallurgica Sinica(English Letters)1993，6，2： | 4 |
| 5 | UAV Maneuvering Decision-Making Algorithm Based on Twin Delayed Deep Deterministic Policy Gradient Algorithm显示文摘Aiming at intelligent decision-making of unmanned aerial vehicle(UAV)based on situation information in air combat,a novelmaneuvering decision method based on deep reinforcement learning is proposed in this paper.The autonomous maneuvering model ofUAV is established byMarkovDecision Process.The Twin DelayedDeep Deterministic Policy Gradient(TD3)algorithm and the Deep Deterministic Policy Gradient(DDPG)algorithm in deep reinforcement learning are used to train the model,and the experimental results of the two algorithms are analyzed and compared.The simulation experiment results show that compared with the DDPG algorithm,the TD3 algorithm has stronger decision-making performance and faster convergence speed and is more suitable for solving combat problems.The algorithm proposed in this paper enables UAVs to autonomously make maneuvering decisions based on situation information such as position,speed,and relative azimuth,adjust their actions to approach,and successfully strike the enemy,providing a new method for UAVs to make intelligent maneuvering decisions during air combat. | Bai Shuangxia Song Shaomei Liang Shiyang Wang Jianmei Li Bo Neretin Evgeny | 2022 | Journal of Artificial Intelligence and Technology2022，2，1： | 3 |
| 6 | Analysis of genes differentially expressed during initial cellular dedifferentiation in cotton显示文摘The early phase of phytohormone induction is a vital stage of somatic embryogenesis. This phase includes a key process for acquiring cellular totipotency through cellular dedifferentiation. To unravel the molecular mechanism of cellular dedifferentiation in cotton, we constructed a cDNA library using the suppression subtractive hybridization method. A total of 286 differential cDNA clones were sequenced and identified. Among these clones, 112 unique ESTs were significantly up-regulated during the early phase of phytohormone induction, and 40.2% of the ESTs were first identified. GST was highly expressed from 6 to 24 h after induction with phytohormone treatment. PRPs were predominantly expressed and exhibited distinct expression patterns in different treatments, suggesting that they are closely related to cellular dedifferentiation in cotton. Putative GhSAMS, GhSAMDC, GhSAHH and GhACO3 involvement in SAM metabolism was identified in this library. The analysis of qRT-PCR showed that two remarkable increased expressions of the four SAM-related genes happened during the early phase of phytohormone induction, and that a highly positive correlation existed between GhSAMS and GhSAHH. The highest expression level of GhSAMS might be associated with its reentry into the cell cycle. The histological observations further showed that some cells accomplished cellular dedifferentiation and division within 72 h in 2,4-D treatment, and that cellular dedifferentiation might be regulated through two alterations in SAM-dependent transmethylation activity in cotton. In addition, the expression patterns of differential genes in different treatments disclosed the complicated interaction between 2, 4-D and kinetin. | ZHU HuaGuo TU LiLi JIN ShuangXia XU Li TAN JiaFu DENG FengLin ZHANG XianLong | 2008 | Chinese Science Bulletin2008，53，23： | 3 |
| 7 | Exogenous melatonin improves the chilling tolerance and preharvest fruit shelf life in eggplant by affecting ROS-and senescence-related processes显示文摘Low temperature is the most common abiotic stress factors during the eggplant cultivation in solar greenhouses.Melatonin plays important roles in plant resistance to low temperature.However,the role of melatonin in regulating chilling tolerance and extending the preharvest shelf life of eggplant fruits is still unknown.In this study,we investigated the effects of exogenous melatonin on eggplant plants and fruits in response to low temperature.Under simulated low-temperature conditions,exogenous melatonin significantly relieved the chilling symptoms of seedlings by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels and relative leakage rates.These reductions were caused by higher superoxide dismutase (SOD) and catalase (CAT) activities and increased endogenous polyamine and melatonin levels compared with those in untreated seedlings.Notably,the expression levels of SOD,CAT1/2,and polyamine synthesis genes (ADC and ODC) were also increased by 100μmol·L~(-1)melatonin,as well as those of genes involved in melatonin synthesis (TDC,T5H,SNAT,ASMT,and COMT) and cold regulation (COR1,CBFa/b,and ZAT2/6/12).To further investigate the effects of melatonin on eggplant leaves and fruits under natural low temperature conditions,100μmol·L~(-1)melatonin was sprayed on the functional leaves at three days before commodity maturation.Melatonin significantly alleviated chilling injury in the leaves and pericarp and extended the preharvest shelf life of the fruit by increasing the expression of COR1,CBF,ZAT2/6/12,and API5 and decreasing the expression of senescence-related genes (NCED1/2 and SAG12).Therefore,100μmol·L~(-1)melatonin improved chilling tolerance and fruit shelf life by upregulating ZAT2/6/12 to affect ROS-and senescence-related processes,which provides a reference for alleviating cold stress and extending the preharvest fruit shelf life in eggplant. | Lijun Song Zhuo Tan Weiwei Zhang Qiang Li Zhaoxiang Jiang Shuxing Shen Shuangxia Luo Xueping Chen | 2023 | Horticultural Plant Journal2023，9，3： | 2 |
| 8 | Red fluorescent protein (DsRed2), an ideal reporter for cotton genetic transformation and molecular breeding显示文摘Genes encoding reporter proteins are used as visual marker-assisted tools in genetic transformation as well as plant breeding. In this study, the red fluorescent protein identified in Discosoma sp. coral(DsRed2) was successfully used as a visual marker for cotton genetic engineering. DsRed2 was successfully expressed in two cotton cultivars,JIN668 and YZ1, driven by the Ca MV-35 S promoter via the Agrobacterium-mediated transformation. Our results suggest that DsRed2 expression provides an early-stage selection tool for the transgenic calli via visual observation. Red fluorescence can be detected not only in callus and somatic embryos but also in most tissues and organs of mature plants. The transgenic line Yz-2-DsRed2 was crossed with four different cotton cultivars to assess the transgene heritability and stability in different genetic backgrounds.The heritability of the red color was highly stable when Yz-2-DsRed2 was used as a male parent. The DsRed2 gene expressed 100% in the F_1 hybrids. To investigate the relationship between DsRed2 transcription and DNA methylation, a methylation-specific PCR approach was applied to the Ca MV-35 S promoter region. The results showed a negative association between DNA methylation level in the promoter region and the transgene transcription.Taken together, these findings suggest DsRed2 a visual reporter gene for cotton genetic transformation and molecular breeding programs. | Lin Sun Muna Alariqi Yi Zhu Jianying Li Zelin Li Qing Wang Yajun Li Hangping Rui Xianlong Zhang Shuangxia Jin | 2018 | The Crop Journal2018，6，4： | 2 |
| 9 | Singular Assembly Configurations and Configuration Bifurcation Characteristics of the Semi-regular Hexagons 6-6 Gough-Stewart Manipulator显示文摘It is well known that singular configurations are inherent to parallel manipulators and have serious influences on their properties. Therefore, these singular configurations should be avoided in the design and application of mechanisms. The researches on the singularity identification and distribution have revealed the relations among the six configuration parameters at singular points. Few works have dealt with the relation between the singularity and the input parameters, as well as the properties of the manipulator nearby the singularity. In this paper, taking the semi-regular hexagons 6-6 Gough-Stewart manipulator (SRHGSMP) as an example, the configuration bifurcation characteristics going with the input parameters, the assembly configurations at singular points, and the reasons to cause the singularity are analyzed. The research reveals that the number and the combination of the input parameters have great influences on the complexity of the singularity and the curvature radiuses of the configuration curves. Under different number of input parameters, the dimensional-utmost singularity, line vectors correlation singularity and Jacobian matrix correlation singularity can occur individually or jointly. Choosing the adjacent input parameters, the simple singularity and the large singularity-free input parameters zones can be obtained. And selecting multiple input parameters, the self-motion regions and the singularity avoidance errors can be reduced. These new discoveries are valuable and of significance for the trajectory design, the singularity avoidance, and the self-motion control of the parallel manipulator. | LI Yutong WANG Yuxin HUANG Zhen PAN Shuangxia | 2009 | Chinese Journal of Mechanical Engineering2009，22，6： | 2 |
| 10 | A comprehensive overview of cotton genomics,biotechnology and molecular biological studies显示文摘Cotton is an irreplaceable economic crop currently domesticated in the human world for its extremely elongated fiber cells specialized in seed epidermis,which makes it of high research and application value.To date,numerous research on cotton has navigated various aspects,from multi-genome assembly,genome editing,mechanism of fiber development,metabolite biosynthesis,and analysis to genetic breeding.Genomic and 3D genomic studies reveal the origin of cotton species and the spatiotemporal asymmetric chromatin structure in fibers.Mature multiple genome editing systems,such as CRISPR/Cas9,Cas12(Cpf1)and cytidine base editing(CBE),have been widely used in the study of candidate genes affecting fiber development.Based on this,the cotton fiber cell development network has been preliminarily drawn.Among them,the MYB-b HLH-WDR(MBW)transcription factor complex and IAA and BR signaling pathway regulate the initiation;various plant hormones,including ethylene,mediated regulatory network and membrane protein overlap fine-regulate elongation.Multistage transcription factors targeting Ces A 4,7,and 8 specifically dominate the whole process of secondary cell wall thickening.And fluorescently labeled cytoskeletal proteins can observe real-time dynamic changes in fiber development.Furthermore,research on the synthesis of cotton secondary metabolite gossypol,resistance to diseases and insect pests,plant architecture regulation,and seed oil utilization are all conducive to finding more high-quality breeding-related genes and subsequently facilitating the cultivation of better cotton varieties.This review summarizes the paramount research achievements in cotton molecular biology over the last few decades from the above aspects,thereby enabling us to conduct a status review on the current studies of cotton and provide strong theoretical support for the future direction. | Xingpeng Wen Zhiwen Chen Zuoren Yang Maojun Wang Shuangxia Jin Guangda Wang Li Zhang Lingjian Wang Jianying Li Sumbul Saeed Shoupu He Zhi Wang Kun Wang Zhaosheng Kong Fuguang Li Xianlong Zhang Xiaoya Chen Yuxian Zhu | 2023 | Science China(Life Sciences)2023，66，10： | 2 |
| 11 | I- dentification of a novel elite genotype for in vitro cul- ture and genetic transformation of cotton显示文摘 | Jin Shuangxia Zhang Xianlong Nie Yichun | 2006 | Biol Plan- tarum2006，50，4： | 1 |
| 12 | Detection of somaclonal variation of cotton(Gossypium hirsutum) using eytogenetics, Flow Cytometry and Molecular Markers显示文摘 | Shuangxia Jin Ramesh Mushke Huaguo Zhu et d | 2008 | Plant Cell Rep2008，27，： | 1 |
| 13 | Development of a new brushless doubly fed doubly salient machine for wind power generation 显示文摘 | FAN Ying CHAU K T NIU Shuangxia | 2006 | IEEE Transactions on Magnetics2006，42，10： | 1 |
| 14 | AGENT based structural static and dynamic collaborative optimization显示文摘 | FENG PEIEN QIAN ZHONGYAN PAN SHUANGXIA el al | 2001 | Science in China (Series E)2001，44，5： | 1 |
| 15 | Nonlinear adaptive robust control of single-rod electro-hydraulic actuator with unknown nonlinear parameters显示文摘 | GUAN Cheng PAN Shuangxia | 2009 | IEEE Transactions on Control System Technolo- gy2009，16，3： | 1 |
| 16 | Nonlinear Adaptive Robust Control of Single- rod Electro - hydraulic Actuator with Unknown Nonlinear Parameters显示文摘 | Guan Cheng Pan Shuangxia | | 0，，03： | 1 |
| 17 | Nonlinear adaptive robust control of single-rod electro-hydraulic actuator with unknown nonlinear parameters显示文摘 | GUAN Cheng PAN Shuangxia | 2009 | IEEE Transactions on Control System Technology2009，16，3： | 1 |
| 18 | Release of Hormones from Conjugates:Chloroplast Expression of -Ghicosidase Results in El- evated Phytohormone Levels Associated with Significant Increase in Biomass and Protection from Aphids or Whiteflies Conferred by Sucrose Esters显示文摘 | Shuangxia Jin Anderson Kanagaraj Dheeraj Verma | 2011 | Plant Physiol2011，155，： | 1 |
| 19 | Somatic embryogenesis and plant regeneration in wild cotton 显示文摘 | Sun Yuqiang Xianlong Jin Shuangxia | 2003 | Plant Cell Tissue and Organ Culture2003，75，： | 1 |
| 20 | A novel solid-rotor inductionmotor with skewed slits in radial and axial directions and its per-formance analysis using finite element method 显示文摘 | HO S L NIU Shuangxia FU W N | 2010 | IEEE Transac-tions on Applied Superconductivity2010，20，3： | 1 |
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