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| 1 | A structural view of the antibiotic degradation enzyme NDM-1 from a superbug显示文摘Gram-negative Enterobacteriaceae with resistance to carbapenem conferred by New Delhi metallo-β-lactamase 1(NDM-1)are a type of newly discovered antibioticresistant bacteria.The rapid pandemic spread of NDM-1 bacteria worldwide(spreading to India,Pakistan,Europe,America,and Chinese Taiwan)in less than 2 months characterizes these microbes as a potentially major global health problem.The drug resistance of NDM-1 bacteria is largely due to plasmids containing the blaNDM-1 gene shuttling through bacterial populations.The NDM-1 enzyme encoded by the blaNDM-1 gene hydrolyzes β-lactam antibiotics,allowing the bacteria to escape the action of antibiotics.Although the biological functions and structural features of NDM-1 have been proposed according to results from functional and structural investigation of its homologues,the precise molecular characteristics and mechanism of action of NDM-1 have not been clarified.Here,we report the threedimensional structure of NDM-1 with two catalytic zinc ions in its active site.Biological and mass spectroscopy results revealed that D-captopril can effectively inhibit the enzymatic activity of NDM-1 by binding to its active site with high binding affinity.The unique features concerning the primary sequence and structural conformation of the active site distinguish NDM-1 from other reported metallo-β-lactamases(MBLs)and implicate its role in wide spectrum drug resistance.We also discuss the molecular mechanism of NDM-1 action and its essential role in the pandemic of drug-resistant NDM-1 bacteria.Our results will provide helpful information for future drug discovery targeting drug resistance caused by NDM-1 and related metallo-β-lactamases. | Yu Guo Jing Wang Guojun Niu Wenqing Shui Yuna Sun Honggang Zhou Yaozhou Zhang Cheng Yang Zhiyong Lou Zihe Rao | 2011 | Protein & Cell2011，2，5： | 16 |
| 2 | Contrasting Effects of Ethylene Biosynthesis on Induced Plant Resistance against a Chewing and a Piercing-Sucking Herbivore in Rice显示文摘乙烯是有在草食动物抵抗上对比效果的压力荷尔蒙。然而,这些差别是否植物特定或草食动物特定,仍然保持未知。我们克隆米饭 1-aminocyclopropane-1-carboxylic 酸(ACC ) synthase 基因, OsACS2,其抄本响应由二个重要害虫的机械伤害和群袭是很快起来调整的:有斑纹的茎 borer (SSB ) Chilo suppressalis 和棕色的 planthopper (BPH ) Nilaparvata lugens。OsACS2 (当交流) 的 Antisense 表示减少了得到的乙烯排放,得到 SSB 的胰岛素朊酶禁止者(TrypPI ) 活动,导致 SSB 的不稳定的版本,和 SSB 抵抗。ACC 的外长的申请恢复了 TrypPI 活动和 SSB 抵抗。与 SSB 相对照, BPH 群袭在当交流线增加了不稳定的排放。因此, BPH 喜欢喂并且在上产卵野类型(WT ) 能被归因于二讨厌的 volatiles, 2-heptanone 和 2-heptanol 的 plantsan 效果,那被当交流植物在更高的数量射出。BPH 蜜露排泄被减少,生来的敌人吸引力在当交流线被提高,导致到 BPH 的更高全面的抵抗。这些结果证明发信号的乙烯对咀嚼和一只穿透吸昆虫在米饭防卫回答和电阻上有形成对照的、草食动物特定的效果,并且可以调停在不同的喂的草食动物之间的抵抗交易在米饭的行业协会。 | Jing Lu Jiancai Li Hongping Ju Xiaoli Liu Matthias Erb Xia Wang Yonggen Lou | 2014 | Molecular Plant2014，7，11： | 15 |
| 3 | Cloud-resolving model for weather modification in China显示文摘Weather modification is widely carried out in China.It is important to develop an operational model for weather modification to predict the microphysical features of cloud and precipitation and to help locate possible seeding areas.A two-moment microphysics scheme is developed using a quasi-implicit calculation method.The scheme predicts the evolution of mass as well as the number densities of the five hydrometeor types.Some microphysical processes are specified.The scheme is implemented with mesoscale models that have been run operationally for weather modification in China. | LOU XiaoFeng SHI YueQin SUN Jing XUE LuLin HU ZhiJin FANG Wen LIU WeiGuo | 2012 | Chinese Science Bulletin2012，57，9： | 12 |
| 4 | Microglial migration mediated by ATP-induced ATP release from lysosomes显示文摘Microglia 是在中央神经系统充当活跃有免疫力的防卫的主要形式的高度能动的房间。由免除损坏房间的因素吸引了, microglia 向损坏或感染的地点被招募,在他们涉及退化、再生的回答和房间碎片的 phagocytotic 清理的地方。从损坏神经纸巾的 ATP 版本被建议了调停向损害的地点的 microglial 过程的快速的延期。然而, microglia 的远程的迁居的机制尚待被澄清。这里,我们发现在 microglia 的 lysosomes 响应各种各样的刺激包含丰富的 ATP 和展览 Ca 2+-dependent exocytosis。由建立一在 vitro 趋化性试金有效,我们证明从 microglia 的 释放endogenously 的 ATP 由 ATPγ 的本地 microinjection 被触发; S 为 microglia 的远程的趋化性是批评的,显著地在与导致 lysosome osmodialysis 的一个代理人或在房间对待的 microglia 被禁止的回答源于在 Rab 27a (灰老鼠)缺乏的老鼠,为能分泌的 lysosomes 的 trafficking 和 exocytosis 要求的小 GTPase 。这些结果建议 microglia 由通过 lysosomal exocytosis 自己释放 ATP 对细胞外的 ATP 作出回应,从而提供积极反馈机制为吸引远 microglia 移居向并且在损害的地点积累产生一个远程的细胞外的信号。 | Ying Dou Hang-jun wu Hui-quan Li Song Qin Yin-er Wang Jing Li Hui-fang LOU Zhong Chew Xiao-ming Li Qing-ming Luo Shumin Duan | 2012 | Cell Research2012，22，6： | 10 |
| 5 | Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice显示文摘Jasmonic acid(JA) and related metabolites play a key role in plant defense and growth. JA carboxyl methyltransferase(JMT) may be involved in plant defense and development by methylating JA to methyl jasmonate(Me JA) and thus influencing the concentrations of JA and related metabolites. However, no JMT gene has been well characterized in monocotyledon defense and development at the molecular level. After we cloned a rice JMT gene,Os JMT1, whose encoding protein was localized in the cytosol, we found that the recombinant Os JMT1 protein catalyzed JA to Me JA. Os JMT1 is up-regulated in response to infestation with the brown planthopper(BPH; Nilaparvata lugens). Plants in which Os JMT1 had been overexpressed(oeJMT plants) showed reduced height and yield. These oe-JMT plants also exhibited increased Me JA levels but reduced levels of herbivore-induced JA and jasmonoyl-isoleucine(JAIle). The oe-JMT plants were more attractive to BPH female adults but showed increased resistance to BPH nymphs,probably owing to the different responses of BPH female adults and nymphs to the changes in levels of H_2O_2 and Me JA in oe-JMT plants. These results indicate that Os JMT1,by altering levels of JA and related metabolites, plays a role in regulating plant development and herbivore-induced defense responses in rice. | Jinfeng Qi Jiancai Li Xiu Han Ran Li Jianqiang Wu Haixin Yu Lingfei Hu Yutao Xiao Jing Lu Yonggen Lou | 2016 | Journal of Integrative Plant Biology2016，58，6： | 10 |
| 6 | Interventional endoscopic ultrasound for a symptomatic pseudocyst secondary to gastric heterotopic pancreas显示文摘Heterotopic pancreas(HP) is a relatively uncommon entity that is defined as pancreatic tissue without a true anatomical or vascular connection to the pancreas. HP does not cause symptoms in most cases but can occasionally produce various manifestations, including nausea, vomiting, abdominal pain, and even heterotopic pancreatitis. Here, we report an unusual case in which heterotopic pancreatitis complicated by the formation of a pseudocyst that caused gastric outlet obstruction was diagnosed based on serum hyperamylasemia and findings from endoscopic ultrasonography(EUS)-guided fine needle aspiration(EUS-FNA) cytology. EUS-guided single pigtail stent insertion was successfully performed for recurrent gastric outlet obstruction. The patient has remained healthy and symptom-free during 4 years of surveillance. In the context of the relevant literature, the described case is a rare case of HP complicated by a pseudocyst treated via EUS-FNA and stent insertion. | hang-bin jin lei lu jian-feng yang qi-feng lou jing yang hong-zhang shen xiao-wei tang xiao-feng zhang | 2017 | World Journal of Gastroenterology2017，23，34： | 9 |
| 7 | Genome-wide variants of Eurasian facial shape differentiation and a prospective model of DNA based face prediction显示文摘It is a long-standing question as to which genes define the characteristic facial features among different ethnic groups. In this study, we use Uyghurs, an ancient admixed population to query the genetic bases why Europeans and Han Chinese look different. Facial traits were analyzed based on high-dense 3D facial images; numerous biometric spaces were examined for divergent facial features between European and Han Chinese, ranging from inter-landmark distances to dense shape geometrics. Genome-wide association studies(GWAS) were conducted on a discovery panel of Uyghurs. Six significant loci were identified, four of which, rs1868752, rs118078182, rs60159418 at or near UBASH3B, COL23A1, PCDH7 and rs17868256 were replicated in independent cohorts of Uyghurs or Southern Han Chinese. A prospective model was also developed to predict 3D faces based on top GWAS signals and tested in hypothetic forensic scenarios. | Lu Qiao Yajun Yang Pengcheng Fu Sile Hu Hang Zhou Shouneng Peng Jingze Tan Yan Lu Haiyi Lou Dongsheng Lu Sijie Wu Jing Guo Li Jin Yaqun Guan Sijia Wang Shuhua Xu Kun Tang | 2018 | Journal of Genetics and Genomics2018，45，8： | 7 |
| 8 | Medical services for sports injuries and illnesses in the Beijing 2022 Olympic Winter Games显示文摘BACKGROUND:Beijing 2022 Olympic Winter Games was the second Games held amid the COVID-19 pandemic.To a certain extent,it has altered the way sporting activities operate.There is a lack of knowledge on injury risk and illness occurrence in elite winter sport athletes amid the COVID-19 pandemic.This study aimed to describe the incidence of injuries and illnesses sustained during the XXIV Olympic Winter Games in Beijing from February 4 to 20,2022.METHODS:We recorded the daily number of injuries and illnesses among athletes reported by Beijing 2022 medical staff in the polyclinic,medical venues,and ambulance.We calculated injury and illness incidence as the number of injuries or illnesses occurring during competition or training,respectively,with incidence presented as injuries/illnesses per 100 athlete-days.RESULTS:In total,2,897 athletes from 91 nations experienced injury or illness.Beijing 2022 medical staff reported 326 injuries and 80 illnesses,equaling 11.3 injuries and 2.8 illnesses per 100 athletes over the 17-day period.Altogether,11%of the athletes incurred at least one injury and nearly 3%incurred at least one illness.The number of injured athletes was highest in the skating sports(n=104),followed by alpine skiing(n=53),ice track(n=37),freestyle skiing(n=36),and ice hockey(n=35),and was the lowest in the Nordic skiing disciplines(n=20).Of the 326 injuries,14(4.3%)led to an estimated absence from training or competition of more than 1 week.A total of 52 injured athletes were transferred to hospitals for further care.The number of athletes with illness(n=80)was the highest for skating(n=33)and Nordic skiing(n=22).A total of 50 illnesses(62.5%)were admitted to the department of dentistry/ophthalmology/otolaryngology,and the most common cause of illness was other causes,including preexisting illness and medicine(n=52,65%).CONCLUSION:Overall,11%of athletes incurred at least one injury during the Games,which is similar to the findings during the Olympic Winter Games in 2014 and 2018.Regarding illness,2%of athletes were affected,which is approximately one-third of the number affected in the 2018 Olympic Winter Games. | Peng-da Han Ding Gao Jie Liu Jing Lou Si-jia Tian Hui-xin Lian Sheng-mei Niu Lu-xi Zhang Yong Wang Jin-jun Zhang | 2022 | World Journal of Emergency Medicine2022，13，6： | 7 |
| 9 | A PKS gene, pks-1, is involved in chaetoglobosin biosynthesis, pigmentation and sporulation in Chaetomium globosum显示文摘Chaetomium globosum is one of the most common fungi in nature. It is best known for producing chaetoglobosins; however, the molecular basis of chaetoglobosin biosynthesis is poorly understood in this fungus. In this study, we utilized RNA interference (RNAi) to characterize a polyketide synthase gene, pks-1, in C. globosum that is involved in the production of chaetoglobosin A. When pks-1 was knocked down by RNAi, the production of chaetoglobosin A dramatically decreased. Knock-down mutants also displayed a pigment-deficient phenotype. These results suggest that the two polyketides, melanin and chaetoglobosin, are likely to share common biosynthetic steps. Most importantly, we found that pks-1 also plays a critical role in sporulation. The silenced mutants of pks-1 lost the ability to produce spores. We propose that polyketides may modulate cellular development via an unidentified action. We also suggest that C. globosum pks-1 is unique because of its triple role in melanin formation, chaetoglobosin biosynthesis and sporulation. This work may shed light on chaetoglobosin biosynthesis and indicates a relationship between secondary metabolism and fungal morphogenesis. | HU Yang HAO XiaoRan LOU Jing ZHANG Ping PAN Jiao ZHU XuDong | 2012 | Science China(Life Sciences)2012，55，12： | 7 |
| 10 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders:executive summary and 2019 update on organizational stroke management显示文摘Aim Stroke is characterised by high morbidity,mortality and disability,which seriously affects the health and safety of the people.Stroke has become a serious public health problem in China.Organisational stroke management can significantly reduce the mortality and disability rates of patients with stroke.We provide this evidence-based guideline to present current and comprehensive recommendations for organisational stroke management.Methods A formal literature search of MEDLINE(1 January 1997 through 30 September 2019)was performed.Data were synthesised with the use of evidence tables.Writing group members met by teleconference to discuss data-derived recommendations.The Chinese Stroke Association’s Levels of Evidence grading algorithm was used to grade each recommendation.results Evidence-based guidelines are presented for the organisational management of patients presenting with stroke.The focus of the guideline was subdivided into prehospital first aid system of stroke,rapid diagnosis and treatment of emergency in stroke centre,organisational management of stroke unit and stroke clinic,construction of regional collaborative network among stroke centres and evaluation and continuous improvement of stroke medical quality.Conclusions The guidelines offer an organisational stroke management model for patients with stroke which might help dramatically. | Min Lou Jing Ding Bo Hu Yusheng Zhang Hao Li ZeFeng Tan Yan Wan An-Ding Xu Chinese Stroke Association Stroke Council Guideline Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，3： | 7 |
| 11 | 15-PGDH is reduced and induces apoptosis and cell cycle arrest in gastric carcinoma显示文摘AIM: To investigate the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) in human gastric cancer and it's mechanism in apoptosis and cell cycle arrest. METHODS: Expression of 15-PGDH mRNA and protein was examined by immunohistochemistry, immunocytochemistry, reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting in tissue from human gastric cancer, gastric precancerous state (gastric polyps and atrophic gastritis), normal stomach, and gastric cancer cell lines. The relationship between gastric cancer, gastric precancerous state and 15-PGDH expression was determined. The association between expression of 15-PGDH and various clinicopathological parameters in gastric cancer was evaluated. Human gastric cancer cell line SGC-7901 was transfected with 15-PGDH expression plasmids. The effect of 15-PGDH on the cell cycle was examined by flow cytometry. The effect of 15-PGDH on apoptosis was examined by transmission electron microscopy, flow cytometry and transferasemediated nick end labeling (TUNEL) assay. Expression of cell cycle (p21, p27, p16 and p53) and apoptosis (Survivin, BCL-2, BCL-XL, BAK and BAX) genes was analyzed by RT-PCR. RESULTS: Expression of 15-PGDH mRNA and protein in human gastric cancer tissues was significantly lower than in normal gastric tissues (P < 0.01). Expression in human gastric cancer cell lines MKN-28 and MKN-45 was reduced, and absent in SGC-7901 cells (P < 0.05). Reduction of 15-PGDH expression was also found in precancerous tissues, such as gastric polyps and atrophic gastritis (P < 0.01). There was a significant difference in expression of 15-PGDH among various gastric cancer pathological types (P < 0.05), with or without distant metastasis (P < 0.05) and different TNM stage (P < 0.01). Flow cytometry demonstrated a significant increase in apoptotic cells in SGC-7901 cells transfected with pcDNA3/15-PGDH plasmid for 24 and 48 h (P < 0.01), and an increased fraction of sub-G1 phase after transfection (P < 0.05). TUNEL assay showed an increased Apoptotic Index in cells overexpressing 15-PGDH (P < 0.01). After transfection, expression of proapoptotic genes, such as BAK (P < 0.05), BAX and p53 (P < 0.01), was increased. Expression of antiapoptotic genes was decreased, such as Survivin, BCL-2 and BCL-XL (P < 0.01). Expression of cyclin-dependent kinase inhibitors p21 and p16 (P < 0.01) was significantly upregulated in cells overexpressing 15-PGDH. CONCLUSION: Reduction of 15-PGDH is associated with carcinogenesis and development of gastric carcinoma. 15-PGDH induces apoptosis and cell cycle arrest in SGC-7901 cells. | Li-Hong Lou Da-Dao Jing Yue-Xing Lai Ying-Ying Lu Ji-Kun Li Kai Wu | 2012 | World Journal of Gastroenterology2012，18，10： | 6 |
| 12 | Efficacy of structured education in patients with type 2 diabetes mellitus receiving insulin treatment (结构化教育对接受胰岛素治疗的2型糖尿病患者的效果)显示文摘 | Xiao Hui Guo Li Nong Ji Ju Ming Lu Jie Liu Qing Qing Lou Jing Liu Li Shen Ming Xia Zhang Xiao Feng Lv Ming Jun Gu | 2014 | Journal of Diabetes2014，，4： | 5 |
| 13 | Rod-shaped thiocyanate-induced abnormal band gap broadening in SCN^- doped CsPbBr3 perovskite nanocrystals显示文摘 | Yongbing Lou Yandan Niu Dongwen Yang Qiaoling Xu Yuhang Hu Ying Shen Jing Ming Jinxi Chen Lijun Zhang Yixin Zhao | 2018 | Nano Research2018，11，5： | 5 |
| 14 | Neocartilage formation from predifferentiated human adipose derived stem cells in vivo显示文摘目的:在 vitro 由人的转变生长因素 beta2 ( hTGF beta2 )导致了检验人的脂肪质的导出的干细胞( hASC )的 chondrogenic 潜力,并且到 predifferentiated hASC 能生产的 investigateif ,在 vivo.Methods:hASC 的 neocartilage 是孤立的 fromsubcutaneous 脂肪质的织物并且在有 hTGF beta2.Chondrogenicdifferentiation 的增加的小团有教养是由 RT-PCR 的 assayed ,西方的弄污,染色的 toluidine 蓝色,为骨胶原类型 II.For 染色的andimmuno组织化学在里面 vivo | Xiao-bing JIN Yong-sheng SUN Ke ZHANG Jing WANG Xiao-dong JU Si-quan LOU | 2007 | Acta Pharmacologica Sinica2007，28，5： | 5 |
| 15 | Acrylamide neurotoxicity on the cerebrum of weaning rats显示文摘The mechanism underlying acrylamide-induced neurotoxicity remains controversial. Previous studies have focused on acrylamide-induced toxicity in adult rodents, but neurotoxicity in weaning rats has not been investigated. To explore the neurotoxic effect of acrylamide on the developing brain, weaning rats were gavaged with 0, 5, 15, and 30 mg/kg acrylamide for 4 consecutive weeks. No obvious neurotoxicity was observed in weaning rats in the low-dose acrylamide group(5 mg/kg). However, rats from the moderate- and high-dose acrylamide groups(15 and 30 mg/kg) had an abnormal gait. Furthermore, biochemical tests in these rats demonstrated that glutamate concentration was significantly reduced, and γ-aminobutyric acid content was significantly increased and was dependent on acrylamide dose. Immunohistochemical staining showed that in the cerebral cortex, γ-aminobutyric acid, glutamic acid decarboxylase and glial fibrillary acidic protein expression increased remarkably in the moderate- and high-dose acrylamide groups. These results indicate that in weaning rats, acrylamide is positively associated with neurotoxicity in a dose-dependent manner, which may correlate with upregulation of γ-aminobutyric acid and subsequent neuronal degeneration after the initial acrylamide exposure. | Su-min Tian Yu-xin Ma Jing Shi Ting-ye Lou Shuai-shuai Liu Guo-ying Li | 2015 | Neural Regeneration Research2015，10，6： | 4 |
| 16 | Long-term variations of major atmospheric compositions observed at the background stations in three key areas of China显示文摘Atmospheric compositions and acid rain are closely related to atmospheric environment,weather,and climate change.In this study,the concentration levels and long-term trends of CO_(2),CH_(4),O_(3),PM_(2.5),PM_(10),and acid rain were presented at the Mt.Waliguan global baseline station in Qinghai,Shangdianzi and Lin'an regional background stations in the North China Plain(NCP)and the Yangtze River Delta region(YRD)of China based on the data observed until 2018.Significant increasing trends of CO_(2) were found at all of these stations.At Mt.Waliguan,the concentration levels and growth rate were consistent with the average values in the Northern Hemisphere(NH)and those observed at the Mauna Loa station in Hawaii.The CH_(4) also showed a upward tendency similar to that observed in NH.Compared with the observations at Mt.Waliguan global baseline station,the CO_(2) and CH_(4) mole fractions were higher at Shangdianzi and Lin'an regional background stations,indicating the important contributions of anthropogenic activities to the regional background level of CO_(2) and CH_(4).The annual averaged surface ozone showed increasing trends at Mt.Waliguan and Shangdianzi stations,whereas no clear trend was found at Lin'an station.Similar variations in the patterns of aerosol concentrations at Shangdianzi and Lin'an were found according to the PM_(2.5) or PM_(10) observations.Overall,the aerosol concentrations at both stations shown declining trends since the observations.However,two periods of high particle concentrations occurred in 2006-2007 and 2013-2014.The annual mean aerosol concentrations have continuously decreased after 2013-2014,indicating the effectiveness of pollution control in the NCP and YRD,especially since the implementation of the‘Action Plan for Prevention and Control of Air Pollution’in September 2013.Comparative analysis of aerosol with acid rain showed that the period with high concentrations of aerosols in the early years was consistent with that of severe acid rain pollution,suggesting that both aerosol pollution and severe acid rain are closely related to coal combustion emissions in China.As a result of the control of coal combustion emissions for acid and SO_(2),NO_(x) emissions have contributed more substantially to aerosols due to the rapid growth of vehicle ownership.A comparison of the trends of aerosols with the maximum daily 8-h average of surface ozone(MDA8)at Shangdianzi and Lin'an stations revealed the complicated relationship between aerosol reduction and ozone production,i.e.,together with the continuous improvement of particulate pollution in the NCP and YRD,the MDA8 at both stations showed a decreasing trend at first but subsequently increased after 2014 with aerosols remaining reduced.Such different variation patterns of the MDA8 with PM_(2.5)(or PM_(10))indicated the high challenge of synergic control of aerosol and ozone pollution in China. | ZHANG Yong JIN Jun-Li YAN Peng TANG Jie FANG Shuang-Xi LIN Wei-Li LOU Meng-Yun LIANG Miao ZHOU Qing JING Jun-Shan LI Ya-Nan JIA Xiao-Fang LYU Shan-Shan | 2020 | Advances in Climate Change Research2020，11，4： | 4 |
| 17 | Exercise promotes angiogenesis by enhancing endothelial cell fatty acid utilization via liver-derived extracellular vesicle miR-122-5p显示文摘Background:Angiogenesis constitutes a major mechanism responsible for exercise-induced beneficial effects.Our previous study identified a cluster of differentially expressed extracellular vesicle microRNAs(miRNAs)after exercise and found that some of them act as exerkines.However,whether these extracellular vesicle miRNAs mediate the exercise-induced angiogenesis remains unknown.Methods:A 9-day treadmill training was used as an exercise model in C57BL/6 mice.Liver-specific adeno-associated virus 8 was used to knock down microRNA-122-5p(miR-122-5p).Human umbilical vein endothelial cells were used in vitro.Results:Among these differentially expressed extracellular vesicle miRNAs,miR-122-5p was identified as a potent pro-angiogenic factor that activated vascular endothelial growth factor signaling and promoted angiogenesis both in vivo and in vitro.Exercise increased circulating levels of miR-122-5p,which was produced mainly by the liver and shuttled by extracellular vesicles in mice.Inhibition of circulating miR-122-5p or liver-specific knockdown of miR-122-5p significantly abolished the exercise-induced pro-angiogenic effect in skeletal muscles,and exerciseimproved muscle performance in mice.Mechanistically,miR-122-5p promoted angiogenesis through shifting substrate preference to fatty acids in endothelial cells,and miR-122-5p upregulated endothelial cell fatty-acid utilization by targeting 1-acyl-sn-glycerol-3-phosphate acyltransferase(AGPAT1).In addition,miR-122-5p increased capillary density in perilesional skin tissues and accelerated wound healing in mice.Conclusion:These findings demonstrated that exercise promotes angiogenesis through upregulation of liver-derived extracellular vesicle miR-122-5p,which enhances fatty acid utilization by targeting AGPAT1 in endothelial cells,highlighting the therapeutic potential of miR-122-5p in tissue repair. | Jing Lou Jie Wu Mengya Feng Xue Dang Guiling Wu Hongyan Yang Yan Wang Jia Li Yong Zhao Changhong Shi Jiankang Liu Lin Zhao Xing Zhang Feng Gao | 2022 | Journal of Sport and Health Science2022，11，4： | 4 |
| 18 | Risk management strategies for the 2022 Olympic Winter Games:The Beijing scheme显示文摘As the first international sporting event scheduled since the outbreak of the novel coronavirus disease 2019(COVID-19)pandemic,the Beijing XXIV Olympic Winter Games were officially held in China between the 4th and 20th of February 2022.1 According to the International Olympic Committee(IOC),there were approximately 2900 elite athletes from 91 National Olympic Committees competing in 109 events during the 16 days of the Games.2 In fact,under the conditions of a global pandemic,mass gatherings during large-scale sporting events represent a conducive environment for viral transmission given the close,prolonged,and frequent interactions between participants;this includes an increased risk of transmission of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). | Jie Liu Jing Lou Yong Wang Jinjun Zhang | 2022 | Journal of Sport and Health Science2022，11，5： | 4 |
| 19 | A leaf shape mutant provides insight into PINOID Serine/Threonine Kinase function in cucumber(Cucumis sativus L.)显示文摘Optimizing leaf shape is a major challenge in efforts to develop an ideal plant type. Cucumber leaf shapes are diverse;however, the molecular regulatory mechanisms underlying leaf shape formation are unknown. In this study, we obtained a round leaf mutant(rl) from an ethyl methanesulfonate-induced mutagenesis population. Genetic analysis revealed that a single recessive gene, rl, is responsible for this mutation. A modified Mut Map analysis combined linkage mapping identified a single nucleotide polymorphism within a candidate gene,Csa1 M537400, as the mutation underlying the trait.Csa1 M537400 encodes a PINOID kinase protein involved in auxin transport. Expression of Csa1 M537400 was significantly lower in the rl mutant than in wild type, and it displayed higher levels of IAA(indole-3-acetic acid) in several tissues. Treatment of wild-type plants with an auxin transport inhibitor induced the formation of round leaves,similar to those in the rl mutant. Altered expression patterns of several auxin-related genes in the rl mutant suggest that rl plays a key role in auxin biosynthesis,transport, and response in cucumber. These findings provide insight into the molecular mechanism underlying the regulation of auxin signaling pathways in cucumber,and will be valuable in the development of an ideal plant type. | Mengfei Song Feng Cheng Jing Wang Qingzhen Wei Wenyuan Fu Xiaqing Yu Ji Li Jinfeng Chen Qunfeng Lou | 2019 | Journal of Integrative Plant Biology2019，61，9： | 3 |
| 20 | Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea显示文摘Appressorium is an infection structure of the phytopathogenic fungus Magnaporthe grisea. Analysis of gene ex-pression profiles of appressorium development provides insight into the molecular basis of pathogenicity and control of this fungal plant disease. A cDNA array representing 2927 unique genes based on a large EST (expressed sequence tag) database of M. grisea strain Y34 was constructed and used to profile the gene expression patterns at mycelium and appressorium maturation stages. Compared with mycelia, 55 up-regulated and 22 down-regulated genes were identified in mature appressoria. Among 77 genes, 16 genes showed no similarity to the genome sequences of M. grisea. A novel homologue of peptidyl-prolyl cis-trans isomerase was found to be expressed at low-level in mature appressoria of M. grisea. The results indicated that the genes such as pyruvate car-boxylase, phospholipid metabolism-related protein and glyceraldehyde 3-phosphate dehydrogenase involved in gluconeogenesis, lipid metabolism and glycolysis, showed differential expression in mature appressoria. Furthermore, genes such as PTH11, beta subunit of G protein and SGT1 involved in cell signalling, were expressed differentially in mature appressoria. Northern blot analysis was used to confirm the cDNA array results. | JIN Qing-chao DONG Hai-tao PENG You-liang CHEN Bao-shan SHAO Jing DENG Ye DAI Cheng-en FANG Yong-qi LOU Yi-chun LI You-zhi LI De-bao | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，2： | 3 |
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