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| 1 | Role of JNK activation in apoptosis:Adouble-edged sword显示文摘JNK is a key regulator of many cellular events, including programmed cell death (apoptosis). In the absence of NF- κB activation, prolonged JNK activation contributes to TNF-α induced apoptosis. JNK is also essential for UV induced apoptosis. However, recent studies reveal that JNK can suppress apoptosis in IL-3-dependent hematopoietic cells via phosphorylation of the proapoptotic Bcl-2 family protein BAD. Thus, JNK has pro- or antiapoptotic functions, depend- ing on cell type, nature of the death stimulus, duration of its activation and the activity of other signaling pathways. | JingLIU AnningLIN | 2005 | Cell Research2005，15，1： | 74 |
| 2 | Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells显示文摘Aim: To investigate the effects of triptolide, a diterpenoid triepoxide from Tripterygium wilfordii Hook F (TWHF), on the co-stimulatory molecule expression and interleukin- 12 (IL- 12) production from THP- 1 cells. Methods: THP- 1 cells were differentiated into macrophage-like cells by Me2SO, and then cultured with IFN-γ(500 kU/L) and lipopolysaccharide (LPS) (1 mg/L) with or without triptolide. The surface molecule expressions were analyzed on a FACScan flow cytometer. IL- 12p40, IL-12p70 were assayed by ELISA. Results: Tripolide suppressed CD80 and CD86 expressions on IFN-γ(500 kU/L) and LPS (1 mg/L) activated THP- 1 cells at nontoxic dosages of 2.5-0.625 ug/L. Furthermore, the production of IL-12p40 and IL-12p70 were also significantly reduced in THP-1 cells exposed to triptolide. Conclusion: Triptolide impairs the antigen-presenting function by inhibiting CD80 and CD86 expressions and decreased IL-12p40 and IL-12p70 (bioactive form) productions from the activated THP-1 cells. | JingLIU Qing-liWU Yong-hongFENG Yi-fuYANG Xiao-yuLI Jian-pingZUO | 2005 | Acta Pharmacologica Sinica2005，26，2： | 13 |
| 3 | A candidate DNA vaccine elicits HCV specific humoral and cellular immune responses显示文摘AIM: TO investigate the immunogenicity of candidate DNA vaccine against hepatitis C virus (HCV) delivered by two plasmids expressing HCV envelope protein 1 (El) and envelope protein 2 (E2) antigens respectively and to study the effect of CpG adjuvant on this candidate vaccine.METHODS: Recombinant plasmJds expressing HCV EI and E2 antigens respectively were used to simultaneously inoculate mice with or without CpG adjuvant. Antisera were then collected and tJters of antJ-HCV antibodies were analyzed by ELISA. One month after the last injection, animals were sacrificed to prepare single-cell suspension of splenocytes.These cells were subjected to HCV antigen specific proliferaion assays and cytokine secretion assays to evaluate the cellular immune responses of the vaccinated animals.RESULTS: Antibody responses to HCV EI and E2 antigens were detected in vaccinated animals. Animals receiving CpG adjuvant had slightly lower titers of anti-HCV antibodies in the sera, while the splenocytes from these animals showed higher HCV-antigen specific proliferation. Analysis of cytokine secretion from the splenocytes was consistent with the above results. While no antigen-specific IL-4 secretion was detected for all vaccinated animals, HCV antigen-specific INF-γ, secretion was detected for the splenocytes of vaccinated animals. CpG adjuvant enhanced the secretion of INF-γ, but did not change the profile of IL-4 secretion.CONCLUSION: Vaccination of mice with plasmids encoding HCV E1 and E2 antigens induces humoral and cellular immune responses. CpG adjuvant significantly enhances the cellular immune response. | Li-XinZhu JingLiu YeYe You-HuaXie Yu-YingKong Guang-DiLi YuanWang | 2004 | World Journal of Gastroenterology2004，10，17： | 12 |
| 4 | Effect of operation-synchronizing transfusion of apoptotic spleen cells from donor rats on acute rejection of recipient rats after liver transplantation显示文摘AIM: To study effect of operation-synchronizing transfusion of apoptotic spleen cells from donor rats on acute rejection of recipient rats after liver transplantation.METHODS: Two of Wistar rats were chosen randomly for normal liver pathology control and ten of SD rats chosen randomly for liver function control as blank group (no operation). The rest of Wistar and SD rats were divided into four groups: control group (only liver transplantation),Dex group (donors receiving intraperitoneal injection of dexamethasone), SpC group (recipients receiving infusion of spleen cells of donors), Dex-SpC group (recipients receiving infusion of apoptotic spleen cells of donors),with each group except blank group, containing 10 SD rats and 10 Wistar rats, respectively. Wistar rats received liver transplantation from SD rats, in the meantime they received infusion of spleen cells of donors, which were induced by an intraperitoneal injection of dexamethasone The serum alanine transaminase (ALT), total bilirubin (T bili), liver pathological changes and survival time were analysed. Statistical analysis was carried out using SPSS 10.0 for Windows. Differences of the parametric data of ALT in means were examined by one-way ANOVA.Differences of ALT between two groups were examined by LSD. Differences of the nonparametric data of T bili in means and scores of pathology classification for acute rejection were examined by Kruskal-Willis H test. The correlations between ALT and T bili were analysed by Bivariate. Kaplan-Meier curves were used to demonstrate survival distribution. The log-rank test was used to compare the survival data.RESULTS: There were significant differences in ALT of the five groups (F= 23.164 P= 0.000), and ALT in DexSpC group was significantly higher than that in blank control, control, Dex, and SpC groups (P = 0.000), and ALT in SpC group was significantly higher than that in blank control (P = 0.000), control (P = 0.004), and Dex groups (P = 0.02). Results of nonparametric analysis of T bili showed that there were differences in T bili of the five groups (χ2 = 33.265 P= 0.000). T bili in Dex-SpC group was significantly higher than that in blank control, control,Dex, and SpC groups. T bili in SpC group was higher than that in blank control, control, and Dex groups. There were significant differences in scores of pathology classification for acute rejection in each of the groups (χ2 = 25.933,P = 0.000). The pathologically more serious acute rejection was found in Dex-SPC group than in other groups. No sign of acute rejection was observed in the blank control group.Slight acute rejection was observed in the control group.Slight-moderate acute rejection was observed in the Dex group. Moderate-acute rejection was observed in the SpC group. Severe-acute rejection was observed in the DexSpC group. The survival time in Dex-SpC group was shorter than in other groups (statistic = 11.13, P = 0.011). ALT and T bili were positively correlated (r= 0.747, P= 0.000,two-tailed).CONCLUSION: In order to reduce quantity of blood loss from rats after liver transplantation, only one of ALT or T bili is needed for liver function measurement of rats.Simultaneous injection of apoptotic spleen cells from donors induced by dexamethasone to liver transplantation rats aggravates acute rejection. One important mechanism of aggravation of acute rejection may be that apoptotic cells are not removed in time and that dead cells including apoptotic cells release inflammatory factors. | JingLiu ShuanWang Er-WeiSun YuWang ZhiZhang Yi-QiangShan Shi-ZhengZhong | 2005 | World Journal of Gastroenterology2005，11，8： | 7 |
| 5 | Plasma-catalytic Selective Reduction of NO with C_(2)H_(4)in the Presence of Excess Oxygen显示文摘This paper reports observations of significant synergistic effects between dielectric barrier discharge (DBD) plasmas and Cu-ZSM-5 catalysts for C2H4 selective reduction of NOx at 250 °C in the presence of excess oxygen by using a one-stage plasma-over-catalyst (POC) reactor. With the reactant gas mixture of 530 ppm NO, 650 ppm C2H4, 5.8% O2 in N2 and GHSV = 12000 h-1, the pure catalytic, pure plasma-induced (discharges over fused silica pellets) and plasma- catalytic (in the POC reactor) NOx conversion are 39%, 1.5% and 79%, respectively. The in-situ optical emission spectra of the reactive systems imply some short-lived active species formed from plasma-induced and plasma-catalytic processes may be responsible to the observed synergistic effects in this one-stage POC system. | QiSUN AiMinZHU XueFengYANG JinHaiNIU YongXU ZhiMinSONG JingLIU | 2005 | Chinese Chemical Letters2005，16，6： | 7 |
| 6 | Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells显示文摘AIM: To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis. METHODS: A carboxyterminal truncated E2 (E2-661) was transiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary (CliO) cell line. Cell proliferation was assessed by ^3H thymidine uptake. Apoptosis was examined by Hoechst 33258 staining, flow cytometry and DNA fragmentation analysis. RESULTS: Reduced proliferation was readily observed in the E2-661 expressing cells. These cells manifested the typical features of apoptosis, including cell shrinkage, chromatin condensation and hypodiploid genomic DNA content. Similar apoptotic cell death was observed in an E2-661 stably expressing cell line. CONCLUSION: HCV E2 can induce apoptosis in cultured mammalian cells. | Li-XinZhu JingLiu You-HuaXie Yu-YingKong YeYe Chun-LinWang Guang-DiLi YuanWang | 2004 | World Journal of Gastroenterology2004，10，20： | 6 |
| 7 | Distribution of T-DNA carrying a Ds element on rice chromosomes显示文摘Over 3000 rice plants with T-DNA carrying a Ds element were constructed by Agro-bacterium tumefaciens mediation. Using inverse PCR methodology, 590 unique right flanking sequences of T-DNA (Ds) were retrieved from independent transformants and classified into six main types on the basis of the origin of filler DNA between the right border of T-DNA and flanking sequence of rice genome. Type I sequences were the most common and showed canonical in-tegration that T-DNA right border was followed by rice genome sequence with or without filler DNA of no more than 50 bp, while type II sequences displayed a vector-genome combination that T-DNA right border was followed by a vector fragment and then connected with rice genome sequence. The location and distribution of 340 type I and II flanking sequences on the rice chromosome were determined using BLAST analysis. The 340 Ds insertions at an average in-terval of 0.8 megabase (Mb) constructed a basic framework of Ds starter points on whole rice chromosomes. The frequency of T-DNA (Ds) inserted into the exons of predicted genes on chromosome one was 21%. Knowledge of T-DNA (Ds) locations on chromosomes will prove to be a useful resource for isolating rice genes by Ds transposon tagging as these Ds insertions can be used as starting lines for further mutagenesis. | WANG Jiang LI Lin WAN Xinshan AN Linsheng ZHANG Jingliu | 2004 | Science China(Life Sciences)2004，47，4： | 5 |
| 8 | Introduction of antisense waxy gene into main parent lines of indica hybrid rice显示文摘Amylose content in rice endosperm is one of the key determinants of rice eating and cooking quality, and the poor quality of indica hybrid rice is closely related to the high amylose level in rice grains. In order to improve the grain quality of the indica hybrid rice by genetic engineering, an antisense fragment of rice waxy gene, driven by the 5’-franking sequences of the rice waxy gene, was successfully introduced into three major parent lines of indica hybrid rice, all contain a high amylose level in the grains, via Agrobacte-rium, and more than 100 hygromycin-resistant plants were regenerated. The analysis of PCR amplification and Southern blots indicated that the T-DNA containing the antisense waxy gene had been integrated into the genome of transgenic rice plants. Most of the primary transgenic rice plants grew normally, and the mature seeds from these transgenic plants were performed for analysis of the amylose content. The results showed that the amylose content in the endosperm of some grains | CHEN Xiuhua LIU Qiaoquan WANG Zongyang WANG Xingwen CAI Xiuling ZHANG Jingliu GU Minghong | 2002 | Chinese Science Bulletin2002，47，14： | 5 |
| 9 | Gene Identification and Expression Analysis of 86,136 Expressed Sequence Tags(EST)from the Rice Genome显示文摘Expressed Sequence Tag(EST)analysis has pioneered genome-wide gene discovery and expression profiling.In order to establish a gene expression index in the rice cultivar indica,we sequenced and analyzed 86,136 ESTs from nine rice cDNA libraries from the super hybrid cultivar LYP9 and its parental cultivars.We assembled these ESTs into 13,232 contigs and leave 8,976 singletons.Overall,7,497 sequences were found similar to the existing sequences in GenBank and 14,711 are novel.These sequences are classified by molecular function,biological process and pathways according to the Gene Ontology.We compared our sequenced ESTs with the publicly available 95,000 ESTs from japonica,and found little sequence variation,despite the large difference between genome sequences,We then assembled the combined 173,000 rice ESTs for further analysis.Using the pooled ESTs,we compared gene expression in metabolism pathway between rice and Arabidopsis according to KEGG.We further profiled gene expression patterns in different tissues,developmental stages,and in a conditional sterile mutant,after checking the libraries are comparable by means of sequence coverage.We also identified some possible library specific genes and a number of enzymes and transcription factors that contribute to rice development. | YanZhou JiabinTang MichaelG.Walker XiuqingZhang JunWang SongnianHu HuayongXu SigiLiu HuanmingYang JunYu JianWang YajunDeng JianhaiDong LinYe LiLin JunLi XuegangWang HaoHu YibinPan WeiLin WeiTian JingLiu LipingWei | 2003 | Genomics, Proteomics & Bioinformatics2003，1，1： | 4 |
| 10 | Transpositional behaviour of the Ds element in the Ac/Ds system in rice显示文摘Insertional mutagenesis based on maize Activator/Dissociator (Ac/Ds) transposons is becoming a ma- jor approach used to produce a saturated mutant collection in rice. In this research, Ds-T-DNA trans- formed homozygotes were crossed with Ac-T-DNA transformed homozygotes in order to establish an Ac/Ds transposon system in rice. The successive investigation of Ds transposition from F1 to F5 gen- erations indicated that the frequencies of germinal transposition increased over successive genera- tions and reached 54.2% in F3 generation. The Ds transposition pattern revealed that a Ds transposition induced an approximately 170-bp deletion of T-DNA sequence and another Ds transposition carried a 272-bp T-DNA sequence. Using thermal asymmetric interlaced PCR (TAIL-PCR), some flanking se- quences of the Ds element were amplified. Analyses of 17 Ds-flanking sequences showed that five Ds were inserted into gene regions. The Ds could transpose not only to the linked sites but also to the unlinked sites. The frequency of inter-chromosomal transposition of Ds was 33.3%. | LIU Fang ZHANG XiangQian ZHANG ZeMin CHEN ZhaoGui ZHU HaiTao WANG Jiang ZHANG JingLiu ZHANG GuiQuan | 2007 | Chinese Science Bulletin2007，52，20： | 4 |
| 11 | cDNA cloning,sequence analysis,and recombinant expression of akitonin beta,a C-type lectin-like protein from Agkistrodon acutus显示文摘AIM: To clone the cDNA of a new member of snake venom C-type lectin-like proteins, to study its structure- function relationships and to achieve its recombinant production. METHODS: PCR primers were designed based on the homology and cDNA was amplified by RT-PCR using total RNA from snake venom gland as the template. The PCR products were cloned into the plasmid pGEM-T and sequenced. The deduced protein sequence was analyzed with some bioinformatic programs. A recombinant expression plasmid was constructed using pBAD- TOPO as vector and transformed into E.coli TOP10 competent cells. RESULTS: A novel cDNA sequence encod- ing akitonin β was found and accepted by GenBank (accession number AF387100). Akitonin β consists of a typical carbohydrate recognition domain (CRD) of C-type lectins, and it is homologous with other snake venom C-type lectin-like proteins. It was predicted to be a platelet antagonist. Upon induction with arabinose rAkitonin β express- ing in E coli was achieved at a high level (superior to 150 mg/L). The recombinant fusion protein exhibited inhibitory activities on rat platelet aggregation in vitro. CONCLUSION: A new member of snake venom C-type lectin-like proteins was discovered and characterized, and an efficient recombinant expression system was estab- lished for its production. | Xiang-dongZHA JingLIU Kang-senXU | 2004 | Acta Pharmacologica Sinica2004，25，3： | 3 |
| 12 | Expression of hepatitis C virus hypervariable region 1 and its clinical significance显示文摘AIM: To explore the properties of hypervariable region 1(HVR1) in the envelope 2 gene of hepatitis C virus by analyzing the reactivity of HVR1 fusion proteins from different Chinese HCV strains with sera of patients with chronic hepatitis C and by comparing their reactivity between interferon therapy responders and non-responders.METHODS: Gene fragments of HVR1 of four HCV strains (three genotype 1b and one genotype 2a) were amplified from pGEMT-E2 plasmids and sub-cloned into pQE40vectors respectively to construct recombinant expression plasmids which expressed HVR1 fused downstream to DHFR in Escherichia coli strain TG1. The purified DHFRHVR1 proteins were then used to detect the anti-HVR1antibodies in 70 serum samples of patients with chronic hepatitis C.RESULTS: Four DHFR- HVR1 fusion proteins were successfully expressed in E.coli (320-800 ug fusion proteins per 100 ml culture). Each fusion protein (SH1b, BJ1b,SD1b and SD2a) reacted with 72.8 % (51/70), 60 % (42/70), 48.6 % (34/70), and 58.6 % (41/70) of the anti-HCV positive patients' sera respectively by ELISA. 57.1% (4/7) of non responders reacted with all four HVR1 fusion proteins, while only 15.3 % (2/13) of responders reacted with all of them. The O.D. values of sera from IFN therapy responders were significantly higher than those of non responders (P<0.05).CONCLUSION: The selected HVR1 fusion proteins expressed in E. coli can broadly react with HCV-infected patients' sera. The intensity and/or quality of the immune response against HCV may be a critical factor determining the response to interferon treatment. With the evolution of virus strains, anti-HVR1 antibodies can not neutralize all the quasispecies. A polyvalent and high immunogenic vaccine comprising a mixture of several HVR1 sequences that cover the reactivity of most HCV isolates may be useful. | Xin-XinZhang Shen-YigZhang JingLiu Zhi-MengLu YuanWang | 2003 | World Journal of Gastroenterology2003，9，5： | 2 |
| 13 | Expression of Hepatitis C Virus E2 Ectodomain in E.coli and Its Application in the Detection of Anti-E2 Antibodies in Human Sera显示文摘The second envelope glycoprotein (E2) of hepatitis C virus has been shown to bind human target cells and has become a major target for the development of anti-HCV vaccines. Anti-E2 antibodies have been suggested to be of clinical significance in diagnosis, treatment and prognosis of hepatitis C. However,large-scale expression and purification of E2 proteins in mammalian cells is difficult. As an alternative, E2 fragment (aa 385-730) with a four-amino-acid mutation (aa 568-571 PCNI to RVTS) was expressed as hexa-histidine-tagged full length protein [E2N730(m)] in E.coli and purified to over 85% purity. Purified E2N730(m) was specifically recognized by homologous hepatitis C patient serum in Western blot, suggesting that it displayed E2-specific antigenicity. Rabbit antiserum raised against E2N730(m) recognized E2 glycoproteins expressed in mammalian cells in Western blot. Purified E2N730(m) was ttsed to detect anti-E2 antibodies in human sera and showed better specificity and sensitivity than previously reported C-terminally truncated E2 fragment (aa 385-565). Association between anti-E2 antibodies in patient sera and HCV RNA status was also demonstrated using this E.coli-derived protein. E2N730(m) might serve as an inexpensive alternative to mammalian cell-expressed E2 proteins in clinical and research applications. | JingLIU Xin-XinZHANG Shen-YingZHANG MinLU Yu-YingKONG YuanWANG Guang-DiLI | 2004 | Acta Biochimica et Biophysica Sinica2004，36，1： | 2 |
| 14 | Determination of Neutron Irradiation-Induced Phosphorus Segregation on Grain Boundaries in a P-doped 2.25Cr1Mo Steel显示文摘Irradiation-induced impurity segregation to grain boundaries is one of the important radiation effects on materials.For this reason, phosphorus segregation to prior austenite grain boundaries in a P-doped 2.25Cr1Mo steel subjected to neutron irradiation is examined using field emission gun scanning transmission electron microscopy (FEGSTEM) with energy dispersive X-ray microanalysis (EDX). The steel samples are irradiated around 270 and 400℃, respectively.The irradiation dose rate and dose are ~1.05×10-8 dpa/s and ~0.042 dpa respectively for 270℃ irradiation, and 1.7×10-8 dpa/s and 0.13 dpa respectively for 400℃ irradiation. The FEGSTEM results indicate that there is no apparent phosphorus segregation during 270℃ irradiation but there is some during 400℃ irradiation. | ShenhuaSONG R.G.Faulkner P.E.J.Flewitt DongdongSHEN JingLIU ZexiYUAN | 2004 | Journal of Materials Science & Technology2004，20，1： | 1 |
| 15 | Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit显示文摘 | ZhenYing Shi Jiang Wang XinShan Wan GeZhi Shen XinQi Wang JingLiu Zhang | 2007 | Planta2007，，1： | 1 |
| 16 | High pressure effects on the luminescent properties and crystal structure of magnesium 8—hydroxyquinoline complex显示文摘The fluorescence emission and X-ray diffraction of magenesium 8-hydroxyquinoline complex(Mgq2) have been measured at high pressure up to 14 GPa.It has been found that pressure can influence the emission dramatically.At relative lower pressure(less than 2.5GPa) the fluorescence of Mgq2 changes slightly,but the emission drops quickly with increase of the pressure when the pressure gets higher than 2.5GPa.The results of in situ energy dispersive X-ray diffraction at high pressure with synchrotron radiation indicate that there is a phase transition at about 2.5-3GPa for Mgq2 crystal.2001 Elsevier Science B.V.All rights reserved. | XuefangLu HairongZhang YiLi JingLiu GuoqiangYang | 2001 | Beijing Synchrotron Radiation Facility2001，，2： | 1 |
| 17 | Explaining the Increase in Coro nary Heart Disease Mortality in Beijing Between 1984 and 1999 显示文摘 | JuLia JingLiu DonZhao | 2004 | Circulation2004，110，： | 1 |
| 18 | Kinetics of the Phenotype and Function of Murine Peritoneal Macrophages Following Acute Inflammation显示文摘This study was undertaken to have a better understand for the process and the underlying mechanisms to limit macrophage activation and population of activated macrophages. A comprehensive kinetics of cytokine production was performed in murine peritoneal macrophages recovered from Balb/c mice at various time during the course of an intraperitoneal injection with thioglycollate (TG). The expression of cell surface molecules such as MHC-Ⅰ, MHC-Ⅱ, B7-1 and B7-2 of these macrophages were also determined by flow cytometry. The present findings of our research suggested that the population of activated macrophages and the activation of macrophages (including cytokines production and expression of cell surface functional molecules)were strictly controlled during inflammation process. This is one of the important mechanisms to retain the host homeostasis. Cellular & Molecular Immunology. 2004;1(1):57-62. | QingliWu YonghongFeng YifuYang Jingliu WenliangZhou PeilanHe RuZhou XiaoyuLi JianpingZou | 2004 | Cellular & Molecular Immunology2004，1，1： | 1 |
| 19 | Determination of ethyl carbamate in Chinese yellow rice wine using high‐performance liquid chromatography with fluorescence detection显示文摘 | Ming‐liangFu JingLiu Xiao‐jieLiu Guo‐qingHe Ji‐chengChen | 2010 | International Journal of Food Science & Technology2010，，6： | 1 |
| 20 | Biomass equa- tions for four shrub species in subtropical China 显示文摘 | HUI Qingzeng QI Jingliu ZONG Weifeng | 2010 | J For Res2010，15，： | 1 |
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